Abstract:
:The old Annurca apple cultivar (Malus domestica), particularly appreciated for its peculiar flavor and crispy flesh, was studied in order to preserve its ancient germplasm. Twelve clones of Annurca were analyzed using random amplified polymorphic DNA (RAPD) and simple-sequence repeat (SSR) markers. Two out of 30 RAPD primers and nine out of ten SSR primers were able to discriminate all the clones analyzed. Data were confirmed by measuring DNA content using flow cytometry. The results provide a good procedure to improve germplasm field management, in order to removing redundant material in the Annurca collection. This represents an efficient way to create a data bank in order to preserve the genetic variability of the Annurca cultivar.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Iannaccone M,Palumbo D,Ventimiglia I,Patocchi A,Spigno P,Capparelli Rdoi
10.1007/s10529-006-9234-0subject
Has Abstractpub_date
2007-02-01 00:00:00pages
279-84issue
2eissn
0141-5492issn
1573-6776journal_volume
29pub_type
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