Improved hydrolase activity in barley and reduced malting time by adding phytase as an activator during malting steeping.

abstract：:Eugenitin, a chromone derivative and a metabolite of the endophyte Mycoleptodiscus indicus, at 5 mM activated a recombinant GH11 endo-xylanase by 40 %. The in silico prediction of ligand-binding sites on the three-dimensional structure of the endo-xylanase revealed that eugenitin interacts mainly by a hydrogen bond wi...

journal_title：Biotechnology letters

authors： Andrioli WJ,Damásio AR,Silva TM,da Silva VB,Maller A,Nanayakkara NP,Silva CH,Polizeli ML,Bastos JK

更新日期：2012-08-01 00:00:00

abstract：:The extracellular domains (ECD) of epidermal growth factor receptors, ErbB1, 2, 3 and 4, were designed as soluble dimeric forms. Each ECD was fused to a short hinge region derived from IgG, such that the stable dimer could be formed with disulfide bridges. This hinge-tagged design minimized the molecular weight to app...

journal_title：Biotechnology letters

authors： Otani T,Hashizume T,Nagaoka T,Fukuda T,Tang CK,Salomon DS,Seno M

更新日期：2010-03-01 00:00:00

abstract：:Fluctuating light intensity had a more significant impact on growth of gametophytes of transgenic Laminaria japonica in a 2500 ml bubble-column bioreactor than constant light intensity. A fluctuating light intensity between 10 and 110 microE m(-2) s(-1), with a photoperiod of 14 h:10 h light:dark, was the best regime ...

journal_title：Biotechnology letters

authors： Gao J,Zhang Y,Zhang W,Wu S,Qin S,Zhang W,Yu X

更新日期：2005-09-01 00:00:00

abstract：OBJECTIVES:The promoter of HpMET3, encoding an ATP sulfurylase, was evaluated for its potential as a repressible promoter to downregulate the expression of target genes in the thermotolerant, methylotrophic yeast Hansenula polymorpha. RESULTS:The expression of lacZ under the control of the 0.6 kb HpMET3 promoter was e...

journal_title：Biotechnology letters

authors： Yoo SJ,Chung SY,Lee DJ,Kim H,Cheon SA,Kang HA

更新日期：2015-11-01 00:00:00

abstract：OBJECTIVES:To develop a colorimetric assay for ATP based on the blue-pigment synthesising non-ribosomal peptide synthetase (NRPS) BpsA, and to demonstrate its utility in defining the substrate specificity of other NRPS enzymes. RESULTS:BpsA is able to convert two molecules of L-glutamine into the readily-detected blue...

journal_title：Biotechnology letters

authors： Brown AS,Calcott MJ,Collins VM,Owen JG,Ackerley DF

更新日期：2020-12-01 00:00:00

abstract：:High concentrations of 2,4,6-trinitrotoluene (TNT) and related nitroaromatic compounds are commonly found in soil and groundwater at former explosive plants. The bacterium, Raoultella terrigena strain HB, isolated from a contaminated site, converts TNT into the corresponding amino products. Radio-HPLC analysis with [(...

journal_title：Biotechnology letters

authors： Claus H,Perret N,Bausinger T,Fels G,Preuss J,König H

更新日期：2007-03-01 00:00:00

abstract：:The recent progress in knowledge on biochemical properties and functions of phospholipases A(2) in plants paved the way for approving the suitability of these enzymes for commercial use now. The secreted phospholipases A(2), representing one type of phospholipases A(2) occurring in plants, show distinct differences in...

journal_title：Biotechnology letters

authors： Mansfeld J

更新日期：2009-09-01 00:00:00

abstract：:Quercetin was methylated with two O-methyltransferases (OMTs) expressed in E. coli. A construct (RSOMT) was designed to express two OMTs: ROMT-9, which methylates specifically at the 3'-hydroxyl group of quercetin and SOMT-2, which methylates at the 4'-hydroxyl group. Both OMT genes were driven by T7 promoters and had...

journal_title：Biotechnology letters

authors： Kim BG,Shin KH,Lee Y,Hur HG,Lim Y,Ahn JH

更新日期：2005-12-01 00:00:00

abstract：:Virus-like particles (VLPs) are a promising and developing option for vaccination and gene therapy. They are also interesting as shuttles for drug targeting. Currently, several different gene expression systems are available, among which the silkworm expression system is known for its mass production capacity. However...

journal_title：Biotechnology letters

authors： Minkner R,Park EY

更新日期：2018-04-01 00:00:00

abstract：:Brevibacterium linens forms hydrolytic enzymes which can be used to accelerate the ripening of cheese without causing bitterness. B. linens ATCC 9172 was grown to a high cell density (50 g dry wt l-1 after 60 h) in a mineral medium containing lactic acid, soy-peptone and ammonium sulphate by applying a continuous feed...

journal_title：Biotechnology letters

authors： Adamitsch BF,Karner F,Hampel WA

更新日期：2003-05-01 00:00:00

abstract：:Bioreactors are essential in tissue engineering, not only because they provide an in vitro environment mimicking in vivo conditions for the growth of tissue substitutes, but also because they enable systematic studies of the responses of living tissues to various mechanical and biochemical cues. The basic principles o...

journal_title：Biotechnology letters

authors： Chen HC,Hu YC

更新日期：2006-09-01 00:00:00

abstract：:A new irreversible esterification method for carboxylic acids catalyzed by a lipase from Candida antarctica (Novozyme 435) in organic solvents has been developed. The water produced during the process is chemically destroyed by a corresponding ester of acetoacetate, which acts as a sacrificial substrate in this reacti...

journal_title：Biotechnology letters

authors： Jeromin GE,Zoor A

更新日期：2008-05-01 00:00:00

abstract：OBJECTIVE:To develop a peptide probe that could be used for gastric cancer detection via binding to CD44 protein with specificity and affinity. RESULTS:A 12-mer phage peptide library was screened against immobilized CD44 protein. Bound phage counts using ELISA were performed to identify phage clones carrying the most ...

journal_title：Biotechnology letters

authors： Zhang D,Jia H,Wang Y,Li WM,Hou YC,Yin SW,Wang TD,He SX,Lu SY

更新日期：2015-11-01 00:00:00

abstract：:Genetically-coded, fluorescence resonance energy transfer (FRET) biosensors are widely used to study molecular events from single cells to whole organisms. They are unique among biosensors because of their spontaneous fluorescence and targeting specificity to both organelles and tissues. In this review, we discuss the...

journal_title：Biotechnology letters

authors： Li IT,Pham E,Truong K

更新日期：2006-12-01 00:00:00

abstract：:A carboxylesterase gene from thermophilic bacterium, Alicyclobacillus tengchongensis, was cloned and expressed in Escherichia coli BL21 (DE3). The gene coded for a 513 amino acid protein with a calculated molecular mass of 57.82 kDa. The deduced amino acid sequence had structural features highly conserved among serine...

journal_title：Biotechnology letters

authors： Xie Z,Xu B,Ding J,Liu L,Zhang X,Li J,Huang Z

更新日期：2013-08-01 00:00:00

abstract：:A novel cDNA for the flavonoid-3',5'-hydroxylase (F35H) gene was cloned from petals of Phalaenopsis, and designated as Phf35h (accession number DQ148458 in GenBank/EMBL/DDBJ). The genomic clone of Phf35h was isolated by a PCR-based strategy. Nucleotide sequence analysis revealed that its genomic clone contains one int...

journal_title：Biotechnology letters

authors： Wang J,Ming F,Han Y,Shen D

更新日期：2006-03-01 00:00:00

abstract：:Biodesulfurization (BDS) in a bioreactor packed with a catalytic bed of silica containing immobilized Rhodococcus rhodochrous was studied. Various bed lengths and support particle sizes were evaluated for BDS of dibenzothiophene (DBT) and gas oil. The sulfur-containing substrates were introduced separately into the bi...

journal_title：Biotechnology letters

authors： Alejandro Dinamarca M,Orellana L,Aguirre J,Baeza P,Espinoza G,Canales C,Ojeda J

更新日期：2014-08-01 00:00:00

abstract：OBJECTIVE:Microalgae gained interest for potential use as biodiesel producers, since they synthesize and accumulate significant quantities of lipids. The aim of this work was to isolate indigenous microalgae strains from Greek habitats, study their physicochemical growth conditions and finally select the best ones with...

journal_title：Biotechnology letters

authors： Savvides AL,Moisi K,Katsifas EA,Karagouni AD,Hatzinikolaou DG

更新日期：2019-05-01 00:00:00

abstract：:Chloroperoxidase from Caldariomyces fumago catalyses the oxidation of 1,2-dihydronaphthalene to (1R,2R)-(+)-dihydroxytetrahydronaphthalene in homogenous citrate buffer/ionic liquid mixtures, using t-butyl hydroperoxide as O2 donor. It tolerates up to 30 (v/v) 1,3-dimethylimidazolium methylsulfate or 1-butyl-3-methylim...

journal_title：Biotechnology letters

authors： Sanfilippo C,D'Antona N,Nicolosi G

更新日期：2004-12-01 00:00:00

abstract：:Ultraviolet-B (UV-B) radiation has significantly negative effect on cell survival rate (P < 0.01) and positive effect on astaxanthin accumulation (P < 0.01) of Haematococcus pluvialis. H. pluvialis accumulated 3.2 mg/g of astaxanthin when being exposed to 5 W/m(2) of UV-B for 60 min prior to 72 h of high light treatme...

journal_title：Biotechnology letters

authors： Wu Z,Chen G,Chong S,Mak NK,Chen F,Jiang Y

更新日期：2010-12-01 00:00:00

abstract：:ECO-orf27 associated with the cluster of ECO-0501 (LW01) from Amycolatopsis orientalis is deduced to encode a type II thioesterase. Disruption of ECO-orf27 reduced LW01 production by 95 %. Complementation of the disrupted mutant with intact ECO-orf27 restored the production of LW01 suggesting that ECO-orf27 is crucial...

journal_title：Biotechnology letters

authors： Shen Y,Huang H,Zhu L,Luo M,Chen D

更新日期：2012-11-01 00:00:00

abstract：OBJECTIVE:To evaluate the quantity of Spirulina cultured in seawater, salt-tolerant strains were screened out and their growth and antioxidant accumulation were studied in different salt concentrations RESULTS:Salt tolerance of five Spirulina strains were investigated with modified Zarrouk medium (with 200-800 mM NaCl...

journal_title：Biotechnology letters

authors： Liu C,Li LJ,Wu CY,Guo KN,Li JH

更新日期：2016-07-01 00:00:00

abstract：:Mucor circinelloides and Mortierella isabellina hydroxylated dehydroabietic acid (DehA). DehA was converted regio- and stereo-selectively by whole cells of Mr. circinelloides to give 2alpha-hydroxydehydroabietic acid in a 75% molar conversion yield (11 mM from 14.7 mM DehA) after 72 h in the cultivation medium contain...

journal_title：Biotechnology letters

authors： Mitsukura K,Imoto T,Nagaoka H,Yoshida T,Nagasawa T

更新日期：2005-09-01 00:00:00

abstract：PURPOSE OF WORK:To increase the bioproduction of L-phenylacetylcarbinol (PAC), a precursor molecule in the synthesis of the decongestants ephedrine and pseudoephedrine and which suffers from substrate, product, and by-product inhibition, by ensuring that the delivery of the substrate, benzaldehyde, is maintained within...

journal_title：Biotechnology letters

authors： Khan TR,Daugulis AJ

更新日期：2011-01-01 00:00:00

abstract：:Two versions of an optimized gene that encodes human papilloma virus type 16 major protein L1 were designed according to the codon usage frequency of Pichia pastoris. Y16 was highly expressed in both P. pastoris and Hansenula polymorpha. M16 expression was as efficient as that of Y16 in P. pastoris, but merely detecta...

journal_title：Biotechnology letters

authors： Liu C,Yang X,Yao Y,Huang W,Sun W,Ma Y

更新日期：2014-05-01 00:00:00

abstract：:Ochratoxin A is a mycotoxin produced by several Aspergillus and some Penicillium species which may be present in food and feed products. It can be enzymatically hydrolyzed into ochratoxin alpha and L: -beta-phenylalanine, thereby decreasing its toxicity. The ochratoxin A degradation capacity of Aspergillus niger is we...

journal_title：Biotechnology letters

authors： Abrunhosa L,Venâncio A

更新日期：2007-12-01 00:00:00

abstract：:An efficient method for creating a DNA library is presented in which gene mutagenesis and recombination can be introduced by integrating error-prone PCR with a staggered extension process in one test tube. In this process, less than 15 cycles of error-prone PCR are used to introduce random mutations. After precipitate...

journal_title：Biotechnology letters

authors： An Y,Ji J,Wu W,Huang R,Wei Y,Xiu Z

更新日期：2008-07-01 00:00:00

abstract：PURPOSE OF WORK:Thymidine is an important precursor in antiviral drugs. We have enhanced thymidine production in E. coli by eliminating the repressors in the transcription of the gene coding for carbamoyl phosphate synthetase. The operon for carbamoyl phosphate synthetase (CarAB) in the thymidine biosynthesis regulator...

journal_title：Biotechnology letters

authors： Koo BS,Hyun HH,Kim SY,Kim CH,Lee HC

更新日期：2011-01-01 00:00:00

abstract：:The open reading frame of the Δ(6)-desaturase gene was isolated from Mortierella alpina W15 and the gene was cloned into a pPIC3.5K vector. The vector was transformed into Pichia pastoris GS115 and expression was induced with methanol. The Δ(6)-desaturase expressed in P. pastoris GS115 catalyzed the conversion of lino...

journal_title：Biotechnology letters

authors： Liu J,Li D,Yin Y,Wang H,Li M,Yu L

更新日期：2011-10-01 00:00:00

abstract：:Genomic DNA was isolated from as little as 2 mg dry biomass of Magnaporthe grisea by microwave treatment within 30 s. The quantity of DNA was good enough for PCR analysis and Dot blot hybridization. This technique can be used for various studies, such as DNA fingerprinting to study the population structure of the phyt...

journal_title：Biotechnology letters

authors： Tendulkar SR,Gupta A,Chattoo BB

更新日期：2003-11-01 00:00:00