Lipid production from indigenous Greek microalgae: a possible biodiesel source.

abstract：:A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amy...

journal_title：Biotechnology letters

authors： Ge B,Tang Z,Lin L,Ren Y,Yang Y,Qin S

更新日期：2005-06-01 00:00:00

abstract：:The volumetric productivity of the beer fermentation process can be increased by using a higher pitching rate (i.e. higher inoculum size). However, the decreased yeast net growth observed in these high cell density brewery fermentations can adversely affect the physiological stability throughout subsequent yeast gener...

journal_title：Biotechnology letters

authors： Verbelen PJ,Dekoninck TM,Van Mulders SE,Saerens SM,Delvaux F,Delvaux FR

更新日期：2009-11-01 00:00:00

abstract：:An alginate lyase gene, algA, encoding a new poly β-D-mannuronate (polyM)-specific alginate lyase AlgA, was cloned from Pseudomonas sp. E03. The recombinant AlgA with (His)6-tag, consisting of 364 amino acids (40.4 kDa),was purified using Ni-NTA Sepharose. The purified lyase had maximal activity (222 EU/mg) at pH 8 an...

journal_title：Biotechnology letters

authors： Zhu BW,Huang LS,Tan HD,Qin YQ,Du YG,Yin H

更新日期：2015-02-01 00:00:00

abstract：:Environmental stresses are the major cause of crop loss worldwide. Polyamines are involved in plant stress responses. However, the precise role(s) of polyamine metabolism in these processes remain ill-defined. Transgenic approaches demonstrate that polyamines play essential roles in stress tolerance and open up the po...

journal_title：Biotechnology letters

authors： Alcázar R,Marco F,Cuevas JC,Patron M,Ferrando A,Carrasco P,Tiburcio AF,Altabella T

更新日期：2006-12-01 00:00:00

abstract：:A morphological parameter of polygonal index was defined as the ratio of cell adhesion area versus the square of the major cell axis, and cells that had an adhesion area larger than 4000 mum(2) and a polygonal index larger than 0.3 were considered large polygonal cells. Cell morphology tended to change from fibroblast...

journal_title：Biotechnology letters

authors： Takagi M,Kitabayashi T,Koizumi S,Hirose H,Kondo S,Fujiwara M,Ueno K,Hiroaki M,Hosokawa Y,Masuhara H,Wakitani S

更新日期：2008-07-01 00:00:00

abstract：:The current study aimed to assess the inhibitory effect of a DNA aptamer (Apt17) which targeted Salmonella invasion proteinA (SipA). The effect of Apt17, on biofilm formation by two Salmonella enteritidis strains, was tested either separately or in combination with ampicillin at different Sub MIC concentrations. Maxim...

journal_title：Biotechnology letters

authors： Shatila F,Yaşa İ,Yalçın HT

更新日期：2020-10-01 00:00:00

abstract：:A coupled in silico thermodynamic and probabilistic metabolic control analysis methodology was verified by applying it to the glycerol biosynthetic pathway in Saccharomyces cerevisiae. The methodology allows predictions even when detailed knowledge of the enzyme kinetics is lacking. In a metabolic steady state, we fou...

journal_title：Biotechnology letters

authors： Birkenmeier M,Mack M,Röder T

更新日期：2015-02-01 00:00:00

abstract：:The substrate specificity of alpha-chymotrypsin and other serine proteases, trypsin, elastase, proteinase K and subtilisin, towards hydrolysis of various polyesters was examined using poly(L-lactide) (PLA), poly(beta-hydroxybutyrate) (PHB), poly(ethylene succinate) (PES), poly(ethylene adipate) (PEA), poly(butylene su...

journal_title：Biotechnology letters

authors： Lim HA,Raku T,Tokiwa Y

更新日期：2005-04-01 00:00:00

abstract：:The gene for beta-agarase of an Agarivorans sp. JA-1 was expressed in Bacillus subtilis strain DB104 for efficient and economical mass-production of the enzyme. We isolated 360 mg protein with a specific activity of 201 U/mg from the culture broth. The efficiency of production was approximately 130-fold higher than th...

journal_title：Biotechnology letters

authors： Lee DG,Jang MK,Lee OH,Kim NY,Ju SA,Lee SH

更新日期：2008-05-01 00:00:00

abstract：:Lipase-catalyzed synthesis of potential multifunctional ribavirin derivatives was performed in acetone. Divinyl dicarboxylates with different chain lengths (C4, C6, C9, C10) were used as acyl donors and the reactions were catalyzed by lipase immobilized on acrylic resin from Candida antarctica (CAL-B). Ribavirin was r...

journal_title：Biotechnology letters

authors： Liu BK,Wang N,Wu Q,Xie CY,Lin XF

更新日期：2005-05-01 00:00:00

abstract：OBJECTIVES:To investigate the effect of full-length fragment of DNA topoisomerase I gene (TOP1) matrix attachment regions (MARs) originating from the human genome on transgene expression in Chinese hamster ovary (CHO) cells and explore the underlying mechanisms. RESULTS:Results showed that TOP1 MAR cannot only enhance...

journal_title：Biotechnology letters

authors： Jia YL,Guo X,Wang XC,Wang TY

更新日期：2019-07-01 00:00:00

abstract：:An extracellular chitinase from Streptomyces halstedii AJ-7, a broad spectrum antifungal biocontrol agent, was characterized and purified. The apparent molecular weight of the purified protein was 55 kDa, Km value and Vmax of the protein for colloidal chitin were 3.2 mg ml(-1) and 118 micromol h(-1), respectively. The...

journal_title：Biotechnology letters

authors： Joo GJ

更新日期：2005-10-01 00:00:00

abstract：:Fermentation kinetics of Penicillium aculeatum ATCC 10409 demonstrated that fungal growth and dextranase release are decoupled. Inoculation by conidia or mycelia resulted in identical kinetics. Two new isoenzymes of the dextranase were characterized regarding their kinetic constants, pI, MW, activation energy and stab...

journal_title：Biotechnology letters

authors： Erhardt FA,Stammen S,Jördening HJ

更新日期：2008-06-01 00:00:00

abstract：:A very high gravity (VHG) repeated-batch fermentation system using an industrial strain of Saccharomyces cerevisiae PE-2 (isolated from sugarcane-to-ethanol distillery in Brazil) and mimicking industrially relevant conditions (high inoculation rates and low O(2) availability) was successfully operated during fifteen c...

journal_title：Biotechnology letters

authors： Pereira FB,Gomes DG,Guimarães PM,Teixeira JA,Domingues L

更新日期：2012-01-01 00:00:00

abstract：OBJECTIVES:To identify a new member of serine proteases from Deinagkistrodon acutus via phage display technique and appraise its biocatalytic activities. RESULTS:A novel thrombin-like enzyme gene was cloned by screening the phage display library of D. acutus venom gland. The gene has a 783 bp ORF encoding 260 amino ac...

journal_title：Biotechnology letters

authors： Li A,Zhang C,Wang J,Wang J,Jiang H,Li J,Ma X,Zhang W,Lu Y

更新日期：2018-01-01 00:00:00

abstract：:Non-viral vector, pCMV-Pth, with full-length human parathyroid hormone (prepro-hPTH) cDNA was constructed and delivered into the ovariectomized mouse quadriceps to explore the effects of electroporational gene transfer of PTH on the bone. The expression of hPTH in the transfected mice was detected by RT-PCR and radioi...

journal_title：Biotechnology letters

authors： Liu B,Tang J,Ji J,Gu J

更新日期：2003-12-01 00:00:00

abstract：OBJECTIVE:To construct a highly expressed and active MEK1R4F (a constitutively active form of mitogen-activated protein kinase kinase 1) by fusion of soluble adenylate kinase (Adk) tag, resulting in Adk-MEK1R4F protein suitable for preparation of phosphorylated ERK. RESULTS:We fused the Adk to the N-terminus of MEK1R4...

journal_title：Biotechnology letters

authors： Yang F,Li H

更新日期：2017-10-01 00:00:00

abstract：:Two isoforms of laccase produced from the culture supernatant of Pycnoporus sanguineus were partially purified by phenyl-Sepharose chromatography. Molecular masses of the enzymes were 80 kDa (Lac I) and 68 kDa (Lac II). Optimum activity of Lac I was at pH 4.8 and 30 degrees C, and Lac II was at pH 4.2 and 50 degrees C...

journal_title：Biotechnology letters

authors： Garcia TA,Santiago MF,Ulhoa CJ

更新日期：2006-05-01 00:00:00

abstract：OBJECTIVE:Heterotrophic manganese (Mn)-oxidizing microorganisms responsible for biogenic manganese oxide (Bio-MnOx) production are fastidious. Their enrichment is not easily accomplished by merely adding a soluble organic substrate to non-sterile mixed cultures. The objective of this study was to evaluate polycaprolact...

journal_title：Biotechnology letters

authors： Aoki M,Miyashita Y,Tran PT,Okuno Y,Watari T,Yamaguchi T

更新日期：2021-01-26 00:00:00

abstract：:The peptide, Ala-Pro-Ala-Arg (APAR), was selected from the screening of a tetrapeptide combinatorial synthetic library as the ligand for affinity purification of an anti-Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) monoclonal antibody (Mab) developed in mouse ascitis. The affinity chromatographic matrix o...

journal_title：Biotechnology letters

authors： Camperi SA,Iannucci NB,Albanesi GJ,Oggero Eberhardt M,Etcheverrigaray M,Messeguer A,Albericio F,Cascone O

更新日期：2003-09-01 00:00:00

abstract：:To control the genetic quality during the whole process of tissue culture of the traditional Chinese medicinal plant, Saussurea involucrate Kar. et Kir., DNA polymorphisms and genetic variations were investigated using randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers. The gene...

journal_title：Biotechnology letters

authors： Yuan XF,Dai ZH,Wang XD,Zhao B

更新日期：2009-08-01 00:00:00

abstract：:Pleurotus eryngii was transformed using a polyethylene glycol-mediated method. A plasmid, pEPUGH, containing a reporter gene (enhanced green fluorescent protein gene, egfp) and a positive selectable marker gene (hygromycin phosphotransferase gene, hph) was constructed. The fused egfp-hph gene was placed under the cont...

journal_title：Biotechnology letters

authors： Yin Y,Liu Y,Jin H,Wang S,Zhao S,Geng X,Li M,Xu F

更新日期：2012-10-01 00:00:00

abstract：OBJECTIVES:Catalytic efficiency of a nitrogen-doped, mesoporous carbon aerogel cathode catalyst was investigated in a two-chambered microbial fuel cell (MFC) applying graphite felt as base material for cathode and anode, utilizing peptone as carbon source. RESULTS:This mesoporous carbon aerogel containing catalyst lay...

journal_title：Biotechnology letters

authors： Tardy GM,Lóránt B,Lóka M,Nagy B,László K

更新日期：2017-07-01 00:00:00

abstract：:Over-expression of the gene, mshA, coding for mycothiol glycosyl transferase improved the robustness of Corynebacterium glutamicum to various stresses. Intracellular mycothiol (MSH) content was increased by 114 % in WT(pXMJ19-mshA) compared to WT(pXMJ19). Survival rates increased by 44, 39, 90, 77, 131, 87, 52, 47, 57...

journal_title：Biotechnology letters

authors： Liu YB,Chen C,Chaudhry MT,Si MR,Zhang L,Wang Y,Shen XH

更新日期：2014-07-01 00:00:00

abstract：:A domain of epiglucan was synthesized by beta-glucosidases. Two beta-glucosidases, an extracellular beta-glucosidase derived from Sclerotinia sclerotiorum grown on xylose, and a commercial lyophilized preparation of beta-glucosidase from Aspergillus niger, were used to synthesize gluco-oligosaccharides from cellobiose...

journal_title：Biotechnology letters

authors： Smaali MI,Michaud N,Marzouki N,Legoy MD,Maugard T

更新日期：2004-04-01 00:00:00

abstract：OBJECTIVES:Breast cancer is a popular fatal malignant tumor for women with high of rates incidence and mortality. Development of the new approaches for breast cancer targeted diagnosis and chemotherapy is emergently needed by the current clinical practice, the important first step is finding a breast cancer specificall...

journal_title：Biotechnology letters

authors： Jin H,Gao X,Xiao L,He H,Cheng S,Zhang C,Hou Y,Song F,Su X,Gao Q,Lu Z,Yang R,Song X,Yang J,Duan W,Hou Y

更新日期：2021-01-01 00:00:00

abstract：:The inhibition of protein-tyrosine phosphatase 1B (PTP1B) is a potential target for treatment of type 2 diabetes. Vanadium and zinc metal coordinated complexes have insulin-enhancing activities, and while vanadium compounds inhibit PTP1B, little is known on the mode of action of zinc compounds. In this study we develo...

journal_title：Biotechnology letters

authors： Seale AP,de Jesus LA,Kim SY,Choi YH,Lim HB,Hwang CS,Kim YS

更新日期：2005-02-01 00:00:00

abstract：:A new method for fast transformation of competent bacterial cells has been developed. The transformation is induced with cholic acid analogues or saponins which cause reversible disruption of the bacterial membrane. This method shortens the time of transformation without significant loss of transformation efficiency i...

journal_title：Biotechnology letters

authors： Ravnikar M,Irman A,Radić N,Lunder M,Strukelj B

更新日期：2009-12-01 00:00:00

abstract：OBJECTIVE:To construct effective genetic expression parts controlling transcription and translation initiation for synthetic biology and heterologous expression in Corynebacterium glutamicum. RESULT:Twelve highly expressed genes were identified from the proteomic data of C. glutamicum. Their related sequences were use...

journal_title：Biotechnology letters

authors： Zhao Z,Liu X,Zhang W,Yang Y,Dai X,Bai Z

更新日期：2016-12-01 00:00:00

abstract：:A new technique for the direct production of recombinant baculovirus in the silkworm larvae is described. To assess the utility of this method, a combination of Bombyx mori nucleopolyhedroviral genome, transfer vector and Lipofectin was co-injected directly into newly ecdysed fifth instar, silkworm larvae. The recombi...

journal_title：Biotechnology letters

authors： Lee KS,Kim BY,Je YH,Woo SD,Sohn HD,Jin BR

更新日期：2007-01-01 00:00:00