Abstract:
:The inhibition of protein-tyrosine phosphatase 1B (PTP1B) is a potential target for treatment of type 2 diabetes. Vanadium and zinc metal coordinated complexes have insulin-enhancing activities, and while vanadium compounds inhibit PTP1B, little is known on the mode of action of zinc compounds. In this study we developed an automated PTP1B inhibition assay that allows for a rapid assessment of the PTP1B inhibition strength of candidate compounds. Synthetic vanadium(IV) and zinc(II) complexes were evaluated: IC50 values for vanadium complexes ranged from 0.06 to 0.8 microM whereas for zinc compounds, values were above 10 microM. Vanadium sulfate, a non-conjugated inorganic salt, had stronger inhibition activity than any of the conjugated metal complexes.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Seale AP,de Jesus LA,Kim SY,Choi YH,Lim HB,Hwang CS,Kim YSdoi
10.1007/s10529-004-7855-8keywords:
subject
Has Abstractpub_date
2005-02-01 00:00:00pages
221-5issue
4eissn
0141-5492issn
1573-6776journal_volume
27pub_type
杂志文章abstract::The peptide, Ala-Pro-Ala-Arg (APAR), was selected from the screening of a tetrapeptide combinatorial synthetic library as the ligand for affinity purification of an anti-Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) monoclonal antibody (Mab) developed in mouse ascitis. The affinity chromatographic matrix o...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025464918453
更新日期:2003-09-01 00:00:00
abstract::Fermentation kinetics of Penicillium aculeatum ATCC 10409 demonstrated that fungal growth and dextranase release are decoupled. Inoculation by conidia or mycelia resulted in identical kinetics. Two new isoenzymes of the dextranase were characterized regarding their kinetic constants, pI, MW, activation energy and stab...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9659-8
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVES:Enhancement of the potential ability of biomacromolecules to cross cell membranes is a critical step for development of effective therapeutic vaccine especially DNA vaccine against human immunodeficiency virus-1 (HIV-1) infection. The supercharged proteins were known as powerful weapons for delivery of diffe...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-02918-w
更新日期:2020-10-01 00:00:00
abstract:OBJECTIVE:To construct a highly expressed and active MEK1R4F (a constitutively active form of mitogen-activated protein kinase kinase 1) by fusion of soluble adenylate kinase (Adk) tag, resulting in Adk-MEK1R4F protein suitable for preparation of phosphorylated ERK. RESULTS:We fused the Adk to the N-terminus of MEK1R4...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2385-3
更新日期:2017-10-01 00:00:00
abstract::Targeted gene replacement in the thermotolerant yeast Kluyveromyces marxianus KCTC 17555 has been hampered by its propensity to non-homologous end joining (NHEJ). To enhance homologous recombination (HR) by blocking NHEJ, we identified and disrupted the K. marxianus KU80 gene. The ku80 deletion mutant strain (Kmku80∆)...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1576-4
更新日期:2014-10-01 00:00:00
abstract::Cellobiose is a major intermediate from cellulase hydrolysis of pretreated plant biomass. Engineering biocatalysts for direct use of cellobiose could eliminate the need for exogenous β-glucosidase. Additionally, rapid removal of cellobiose in a simultaneous saccharification and fermentation facilitates enzymatic hydro...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1355-7
更新日期:2014-02-01 00:00:00
abstract::Tributyl phosphate (TBP) is widely used in nuclear fuel processing and other waste generating chemical industries. Although TBP is bacteriostatic, some microbes are resistant to it and may degrade it. Under dark aerobiosis, purple non-sulfur photosynthetic bacteria degraded up to 0.6 mM TBP, initially present at 2 mM,...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2882-7
更新日期:2005-04-01 00:00:00
abstract::A purified recombinant enzyme from Spirochaeta thermophila, that is suggested to be a cellobiose 2-epimerase, was a 47 kDa monomer with a specific activity of 29.2 U min(-1) for mannobiose. The epimerization activity of the recombinant enzyme for mannobiose was maximal at pH 7.0 and 60 °C with a half-life of 124 h. Th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1267-6
更新日期:2013-11-01 00:00:00
abstract::Seventeen independent transgenic rice plants with the maize anthocyanin regulatory gene Lc under control of the CaMV 35S promoter were obtained and verified by molecular identification. Ten plants showed red spikelets during early development of florets, and the degenerate florets were still red after heading. Additio...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1046-9
更新日期:2013-01-01 00:00:00
abstract::Two ketoreductases from Candida glabrata were used for the asymmetric reduction of prochiral substituted acetophenones displayed different enantiopreference toward para-, meta-substituted and ortho-halogen substituted acetophenones with excellent enantioselectivity. Homology modeling and docking analysis were in confo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1228-0
更新日期:2013-09-01 00:00:00
abstract::A new ribonuclease (RNase) with tobacco mosaic virus inhibition was isolated and purified from Bacillus cereus ZH14 through ammonium sulfate precipitation, ultrafiltration, ion-exchange chromatography of DEAE-Sephadex A-50 column, and gel chromatography of Sephacryl S-200HR column. The enzyme was purified approximatel...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9831-1
更新日期:2009-01-01 00:00:00
abstract::Human hemangioblasts exist only during the early embryonic developmental stage thereby limiting the adult cellular source from which to obtain such cells for study. To overcome this, hemangioblast studies have focused on utilizing human embryonic stem cell (hESC) derivatives but current methods are cell-line dependent...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1786-4
更新日期:2015-06-01 00:00:00
abstract::Continuous production of fructooligosaccharides (FOS) by Aureobasidium pullulans immobilized on calcium alginate beads with a packed bed was investigated at a plant scale reactor. Optimum conditions were with 770 g sucrose/l, being fed at 200 l/h at 50°C which gave a productivity of 180 g FOS/l h. Initial activity was...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0606-8
更新日期:2011-08-01 00:00:00
abstract::Trichodesmium sp., isolated from the Great Barrier Reef lagoon, was cultured in artificial seawater media containing a range of Fe concentration. Fe additions stimulated growth, N2 fixation, cellular chlorophyll a content, light-saturated chlorophyll a-specific gross photosynthetic capacity (Pmchl a) and the dark resp...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1023068232375
更新日期:2003-04-01 00:00:00
abstract::The Bacillus pumilus SG2 chitinase gene (ChiS) and its truncated form lacking chitin binding (ChBD) and fibronectin type III (FnIII) domains were transformed to Arabidopsis plants and the expression, functionality and antifungal activity of the recombinant proteins were investigated. Results showed that while the two ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0192-1
更新日期:2010-04-01 00:00:00
abstract::Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9898-8
更新日期:2009-04-01 00:00:00
abstract:PURPOSE OF WORK:Thymidine is an important precursor in antiviral drugs. We have enhanced thymidine production in E. coli by eliminating the repressors in the transcription of the gene coding for carbamoyl phosphate synthetase. The operon for carbamoyl phosphate synthetase (CarAB) in the thymidine biosynthesis regulator...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0413-7
更新日期:2011-01-01 00:00:00
abstract::Ramoplanin is a lipopeptide antibiotic active against multi-drug-resistant, Gram-positive pathogens. Structurally, it contains a di-mannose moiety attached to the peptide core at Hpg(11). The biosynthetic gene cluster of ramoplanin has already been reported and the assembly of the depsipeptide has been elucidated but ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1233-3
更新日期:2013-09-01 00:00:00
abstract::Decrystallized chitosan was produced from shrimp shells with a low degree of crystallinity (10%) and a high anionic dye binding capacity. Raw, mixed dye wastewater from a textile factory was efficiently decolorized using decrystallized chitosan that was more efficient than using normal chitosan and activated carbon. D...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024562900548
更新日期:2003-07-01 00:00:00
abstract::Virus-induced gene silencing is based on the sequence-specific degradation of RNA. Here, a gene silencing vector derived from EuMV-YP, named pEuMV-YP:ΔAV1, was used to silence ChlI and NPR1 genes in Nicotiana benthamiana. The silencing of the ChlI transcripts was efficient in the stems, petioles and leaves as reflecte...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1146-1
更新日期:2013-05-01 00:00:00
abstract:OBJECTIVE:To produce (S)-3-hydroxy-1-(3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl]-4-(2,4,5-trifluorophenyl)butan-1-one (S)-1 from 4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro [1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl)-1-(2,4,5-trifluorophenyl)butan-2-one (2) by microbial bioreduction. RESULTS:A new...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2051-1
更新日期:2016-05-01 00:00:00
abstract::In the industrial production of bioethanol from lignocellulosic biomass, a strain of Saccharomyces cerevisiae that can ferment xylose in the presence of inhibitors is of utmost importance. The recombinant, industrial-flocculating S. cerevisiae strain NAPX37, which can ferment xylose, was used as the parent to delete t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1581-7
更新日期:2014-10-01 00:00:00
abstract::C3H/HeJ mice were sensitized with ovalbumin (OVA) and choleratoxin (CT) for 5 weeks, and then Bifidobacterium bifidum BGN4 was administered continuously for 7 weeks, starting 2 weeks before (pre-treatment group) and 2 weeks after (post-treatment group) the initial sensitization. After sensitization, the OVA-induced (s...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3682-9
更新日期:2005-09-01 00:00:00
abstract:OBJECTIVES:To optimize the production of active inclusion bodies (IBs) containing human D-amino acid oxidase (hDAAO) in Escherichia coli. RESULTS:The optimized initial codon region combined with the coexpressed rare tRNAs, fusion of each of the N-terminal partners including cellulose-binding module, thioredoxin, gluta...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2413-3
更新日期:2017-11-01 00:00:00
abstract::Bacterial and fungal contaminants of enset (Ensete ventricosum) cultures and microbes associated with surface-sterilized field material were identified by 16S/26S rDNA sequencing. Ten bacterial species were identified in 16 isolates from in vitro cultures and seven in 10 isolates from field clones. Three yeast species...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-6032-4
更新日期:2004-12-01 00:00:00
abstract::The extracellular domains (ECD) of epidermal growth factor receptors, ErbB1, 2, 3 and 4, were designed as soluble dimeric forms. Each ECD was fused to a short hinge region derived from IgG, such that the stable dimer could be formed with disulfide bridges. This hinge-tagged design minimized the molecular weight to app...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0160-9
更新日期:2010-03-01 00:00:00
abstract::The genus Ajuga L., belonging to Lamiaceae family, is widespread. The demand for Ajuga species has risen sharply because of their medicinal, ornamental, and pharmacological properties. These wide-ranging plants are being rapidly depleted due to over-collection for ornamental and medicinal purposes, as well as by habit...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-017-2376-4
更新日期:2017-09-01 00:00:00
abstract::Kluyveromyces marxianus was grown in submerged culture in a complex medium with several potential inducers of lipolytic activity (triacylglycerols, fatty acids). The highest extracellular lipolytic enzyme production (about 80 U ml(-1) in 3 d) was obtained when the medium was supplemented with 2 g urea l(-1) plus 5 g t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025049825720
更新日期:2003-09-01 00:00:00
abstract::Metabolic regulation in Escherichia coli was studied in terms of the changes in the expression of the global regulatory genes rpoD, rpoS, soxRS, cra, fadR, iclR and arcA at three different growth phases, in batch culture. The expression of rpoS and several rpoS-dependent metabolic pathway genes, such as tktB, talA, fu...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9621-1
更新日期:2008-05-01 00:00:00
abstract::A single-compartmented microbial fuel cell composed of a graphite felt anode modified with Neutral Red (NR-anode) and a porous Fe(II)-carbon cathode (FeC-cathode) were compared for electricity generation from Microbacterium sp. and Pseudomonas sp. under identical conditions. Pseudomonas sp. was more than four times th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0184-1
更新日期:2010-04-01 00:00:00