Highly enantioselective production of a chiral intermediate of sitagliptin by a novel isolate of Pseudomonas pseudoalcaligenes.

abstract：:Gluconobacter oxydans LMG 1489 was selected as the best strain for NAD(P)-dependent polyol dehydrogenase production. The highest enzyme activities were obtained when this strain was cultivated on a medium consisting of 30 g glycerol l(-1), 7.2 g peptone l(-1) and 1.8 g yeast extract l(-1). Two D-fructose reducing, NAD...

journal_title：Biotechnology letters

authors： Parmentier S,Beauprez J,Arnaut F,Soetaert W,Vandamme EJ

更新日期：2005-03-01 00:00:00

abstract：:The production of a low-temperature alkalophilic lipase from Acinetobacter johnsonii was improved using genome shuffling. The starting populations, obtained by UV irradiation and diethyl sulfate mutagenesis, were subjected to recursive protoplast fusion. The optimal conditions for protoplast formation and regeneration...

journal_title：Biotechnology letters

authors： Wang H,Zhang J,Wang X,Qi W,Dai Y

更新日期：2012-01-01 00:00:00

abstract：:Long-term cell recycle fermentations of Candida tropicalis were performed over 14 rounds of fermentation. The average xylitol concentrations, fermentation times, volumetric productivities and product yields for 14 rounds were 105 g l(-1), 333 h, 4.4 g l(-1) h(-1) and 78%, respectively, in complex medium; and 110 g l(-...

journal_title：Biotechnology letters

authors： Kim TB,Lee YJ,Kim P,Kim CS,Oh DK

更新日期：2004-04-01 00:00:00

abstract：:Deacetoxy/deacetylcephalosporin C synthase (acDAOC/DACS) from Acremonium chrysogenum is a bifunctional enzyme that catalyzes both the ring-expansion of penicillin N to deacetoxycephalosporin C and the hydroxylation of the latter to deacetylcephalosporin C. The R308 residue located in close proximity to the C-terminus ...

journal_title：Biotechnology letters

authors： Wu XB,Tian XY,Ji JJ,Wu WB,Fan KQ,Yang KQ

更新日期：2011-04-01 00:00:00

abstract：:The genus Ajuga L., belonging to Lamiaceae family, is widespread. The demand for Ajuga species has risen sharply because of their medicinal, ornamental, and pharmacological properties. These wide-ranging plants are being rapidly depleted due to over-collection for ornamental and medicinal purposes, as well as by habit...

journal_title：Biotechnology letters

authors： Park HY,Kim DH,Sivanesan I

更新日期：2017-09-01 00:00:00

abstract：:The astaxanthin biosynthesis gene cluster in Paracoccus haeundaensis consists of six genes: crtW, crtZ, crtY, crtI, crtB, and crtE contain 726, 486, 1158, 1503, 912, and 879 base pairs, respectively. Individual carotenoid biosynthesis genes of P. haeundaensis have now been expressed in E. coli and each gene product ha...

journal_title：Biotechnology letters

authors： Lee JH,Kim YT

更新日期：2006-08-01 00:00:00

abstract：:The penicillin G amidase (PGA) from Alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in Escherichia coli. The corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter....

journal_title：Biotechnology letters

authors： Deak PM,Lutz-Wahl S,Bothe H,Fischer L

更新日期：2003-03-01 00:00:00

abstract：:The Rho1 protein is a GTPase that participates in cell wall biogenesis. We analyzed the transcript levels of laccase genes (lccl, lcc2, lcc3, lcc4, lcc5, and lcc9), and a xylanase gene (xyl3) in Fusarium oxysporum f. sp. lycopersici strain 4287 (wild type) and two mutant strains; rhol::hyg that lacks a functional Rho1...

journal_title：Biotechnology letters

authors： Reyes-Medina MA,Macías-Sánchez KL

更新日期：2015-03-01 00:00:00

abstract：:Different strains of Micrococcus luteus, isolated from high-altitude Argentinean wetlands, were recently reported to harbour the linear plasmids pLMA1, pLMH5 and pLMV7, all of which with 5'-covalently attached terminal proteins. The link between pLMA1 and the host's erythromycin resistance as well as further presumpti...

journal_title：Biotechnology letters

authors： Wagenknecht M,Dib JR,Thürmer A,Daniel R,Farías ME,Meinhardt F

更新日期：2010-12-01 00:00:00

abstract：:The genetic relationship among commercial cultivars of Citrus limon (lemon) was analysed by inter-simple sequence repeats (ISSR) and flow cytometry techniques. Two cultivars with a close germplasm were distinguished by screening 10 SSR primers and by measuring DNA content of prestained nuclei. ...

journal_title：Biotechnology letters

authors： Capparelli R,Viscardi M,Amoroso MG,Blaiotta G,Bianco M

更新日期：2004-08-01 00:00:00

abstract：:The biomacromolecule, gelatin, has increasingly been used in biomedicine-beyond its traditional use in food and cosmetics. The appealing advantages of gelatin, such as its cell-adhesive structure, low cost, off-the-shelf availability, high biocompatibility, biodegradability and low immunogenicity, among others, have m...

journal_title：Biotechnology letters

authors： Su K,Wang C

更新日期：2015-11-01 00:00:00

abstract：:Fermentation kinetics of Penicillium aculeatum ATCC 10409 demonstrated that fungal growth and dextranase release are decoupled. Inoculation by conidia or mycelia resulted in identical kinetics. Two new isoenzymes of the dextranase were characterized regarding their kinetic constants, pI, MW, activation energy and stab...

journal_title：Biotechnology letters

authors： Erhardt FA,Stammen S,Jördening HJ

更新日期：2008-06-01 00:00:00

abstract：:Chlorella protothecoides, a lutein-producing microalga, was grown aerobically in a mineral medium prepared with 70% (v/v) deuterated water. HPLC/atmospheric pressure chemical ionization-mass spectrometry (HPLC/APCI-MS) analysis revealed approximately 58% replacement of hydrogen by deuterium atoms as indicated by the m...

journal_title：Biotechnology letters

authors： Bhosale P,Serban B,Bernstein PS

更新日期：2006-09-01 00:00:00

abstract：:Virus-induced gene silencing is based on the sequence-specific degradation of RNA. Here, a gene silencing vector derived from EuMV-YP, named pEuMV-YP:ΔAV1, was used to silence ChlI and NPR1 genes in Nicotiana benthamiana. The silencing of the ChlI transcripts was efficient in the stems, petioles and leaves as reflecte...

journal_title：Biotechnology letters

authors： Villanueva-Alonzo HJ,Us-Camas RY,López-Ochoa LA,Robertson D,Guerra-Peraza O,Minero-García Y,Moreno-Valenzuela OA

更新日期：2013-05-01 00:00:00

abstract：:The ion-exchange chromatography behavior of recombinant glucose dehydrogenase harboring pyrroloquinoline quinone (PQQGDH) was modified to greatly simplify its purification. The surface charge of PQQGDH was engineered by either fusing a three-arginine tail to the C-terminus of PQQGDH (PQQGDH+Arg3) or by substituting th...

journal_title：Biotechnology letters

authors： Koh H,Igarashi S,Sode K

更新日期：2003-10-01 00:00:00

abstract：:Human hemangioblasts exist only during the early embryonic developmental stage thereby limiting the adult cellular source from which to obtain such cells for study. To overcome this, hemangioblast studies have focused on utilizing human embryonic stem cell (hESC) derivatives but current methods are cell-line dependent...

journal_title：Biotechnology letters

authors： Gil CH,Lee JH,Seo J,Park SJ,Park Z,Kim J,Jung AR,Lee WY,Kim JS,Moon SH,Lee HT,Chung HM

更新日期：2015-06-01 00:00:00

abstract：:We report a novel and convenient method for the determination of glycoproteins, especially antibodies, using galactose oxidase (GAO) on the basis of the contents of galactosyl and N-acetylgalactosaminyl residues in carbohydrate chains of glycoproteins. GAO converts galactose residues to their corresponding aldehyde an...

journal_title：Biotechnology letters

authors： Jeon SI,Hong JW,Yoon HC

更新日期：2006-09-01 00:00:00

abstract：:Avian myeloblastosis virus reverse transcriptase (AMV RT) is a heterodimer consisting of a 63 kDa α-subunit and a 95 kDa β subunit. Moloney murine leukaemia virus reverse transcriptase (MMLV RT) is a 75 kDa monomer. These two RTs are the most extensively used for conversion of RNA to DNA. We previously developed sever...

journal_title：Biotechnology letters

authors： Konishi A,Yasukawa K,Inouye K

更新日期：2012-07-01 00:00:00

abstract：:Our current knowledge of protein unfolding is overwhelmingly related to reversible denaturation. However, to engineer thermostable enzymes for industrial applications and medical diagnostics, it is necessary to consider irreversible denaturation processes and/or the entire quaternary structure. In this study we have u...

journal_title：Biotechnology letters

authors： Kaneko H,Minagawa H,Shimada J

更新日期：2005-11-01 00:00:00

abstract：:An open reading frame (ORF) encoding chitin oligosaccharide deacetylase (Pa-COD) gene and its signal sequence was cloned from the Vibrio parahaemolyticus KN1699 genome and its sequence was analyzed. The ORF encoded a 427 amino acid protein, including the 22 amino acid signal sequence. The deduced amino acid sequence w...

journal_title：Biotechnology letters

authors： Kadokura K,Sakamoto Y,Saito K,Ikegami T,Hirano T,Hakamata W,Oku T,Nishio T

更新日期：2007-08-01 00:00:00

abstract：:We proposed a novel combined gene therapy of human vascular endothelial growth factor 165 gene (hVEGF165) and human bone morphogenetic protein 2 gene (hBMP2) for bone regeneration by lentivirus-mediated co-transfection of both genes into rat bone marrow-derived mesenchymal stromal cells (MSCs). Both genes were success...

journal_title：Biotechnology letters

authors： Jiang J,Fan CY,Zeng BF

更新日期：2008-02-01 00:00:00

abstract：:A novel two-step fermentation process was developed to enhance arachidonic acid (ARA) production by Mortierella alpina ME-1 in a 5 l fermentor. Agitation speed and aeration rate were adjusted from 180 to 40 rpm and from 0.6 to 1 vvm, respectively, after 5 days cultivation, to decrease physical damage to the mycelia an...

journal_title：Biotechnology letters

authors： Jin MJ,Huang H,Xiao AH,Zhang K,Liu X,Li S,Peng C

更新日期：2008-06-01 00:00:00

abstract：:The aim of this study was to characterize the growth of the fungus Leucoagaricus gongylophorus LEU18496, isolated from the fungus garden of the nest of leaf cutter ants Atta mexicana. The fungus garden was cultivated in an artificial laboratory nest and the fungus further grown in submerged (SmC) and solid state (SSC)...

journal_title：Biotechnology letters

authors： Maya-Yescas ME,Revah S,Le Borgne S,Valenzuela J,Palacios-González E,Terrés-Rojas E,Vigueras-Ramírez G

更新日期：2021-01-02 00:00:00

abstract：:Baker's yeast suspensions were incubated at different pressures (from 1 bar to 6 bar) and different gases [air, O(2) and a mixture of 8% (v/v) CO(2), 21% O(2) and N(2)]. Raising the air pressure from 1 bar to 6 bar stimulated cell growth but had no effect on leavening ability or viability of the cells. A 50% reduction...

journal_title：Biotechnology letters

authors： Campelo AF,Belo I

更新日期：2004-08-01 00:00:00

abstract：OBJECTIVES:To design novel 3D in vitro co-culture models based on the RGD-peptide-induced cell self-assembly technique. RESULTS:Multicellular spheroids from M-3 murine melanoma cells and L-929 murine fibroblasts were obtained directly from monolayer culture by addition of culture medium containing cyclic RGD-peptide. ...

journal_title：Biotechnology letters

authors： Akasov R,Gileva A,Zaytseva-Zotova D,Burov S,Chevalot I,Guedon E,Markvicheva E

更新日期：2017-01-01 00:00:00

abstract：:Two genes, MGFc (40) and MGFc (24), encoding different E peptides of mechano-growth factor (MGF), were obtained by a four-step PCR strategy and subcloned into pRSETC and pGEX-6p-1. Recombinant MGFc(40) protein (4 mg l(-1)) was expressed and purified by affinity chromatog...

journal_title：Biotechnology letters

authors： Song L,Zhu WS,Dong JL,Li ZY,Liu XH

更新日期：2012-02-01 00:00:00

abstract：:Alginate production by Azotobacter vinelandii growing in chemostat cultures was evaluated under different O(2) transfer rates (OTR). As a result of modifying the culture's agitation rate from 300 to 500 rpm, the OTR increased from 9 to 15.1 mmol l(-1) h(-1) and a slight variation in the alginate production (1.7-2.2 g ...

journal_title：Biotechnology letters

authors： Díaz-Barrera A,Silva P,Avalos R,Acevedo F

更新日期：2009-06-01 00:00:00

abstract：:We truncated the VP2 protein of infectious bursal disease virus into five fragments: V1-5. All fragments were displayed on the inner membrane of the Escherichia coli periplasm. After disruption of the outer membrane, spheroplasts that had anchored with the VP2 fragment were incubated with an anti-VP2 polyclonal antibo...

journal_title：Biotechnology letters

authors： Guo M,Xu LM,Zhou B,Yin JC,Ye XL,Ren GP,Li DS

更新日期：2014-03-01 00:00:00

abstract：:Solubilization of lignin and carbohydrates from the lignin-holocellulose structure of cotton seed-coat fragments was investigated by UV/VIS spectrometry. Xylanase (Pulpzyme HC) pre-treatment partially destroyed the lignocellulosic structure of the seed-coat fragments, producing reducing sugars and soluble lignin in th...

journal_title：Biotechnology letters

authors： Csiszár E,Losonczi A,Koczka B,Szakács G,Pomlényi A

更新日期：2006-05-01 00:00:00

abstract：:Lipase-catalyzed synthesis of potential multifunctional ribavirin derivatives was performed in acetone. Divinyl dicarboxylates with different chain lengths (C4, C6, C9, C10) were used as acyl donors and the reactions were catalyzed by lipase immobilized on acrylic resin from Candida antarctica (CAL-B). Ribavirin was r...

journal_title：Biotechnology letters

authors： Liu BK,Wang N,Wu Q,Xie CY,Lin XF

更新日期：2005-05-01 00:00:00