Abstract:
:The biomacromolecule, gelatin, has increasingly been used in biomedicine-beyond its traditional use in food and cosmetics. The appealing advantages of gelatin, such as its cell-adhesive structure, low cost, off-the-shelf availability, high biocompatibility, biodegradability and low immunogenicity, among others, have made it a desirable candidate for the development of biomaterials for tissue engineering and drug delivery. Gelatin can be formulated in the form of nanoparticles, employed as size-controllable porogen, adopted as surface coating agent and mixed with synthetic or natural biopolymers forming composite scaffolds. In this article, we review recent advances in the versatile applications of gelatin within biomedical context and attempt to draw upon its advantages and potential challenges.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Su K,Wang Cdoi
10.1007/s10529-015-1907-0subject
Has Abstractpub_date
2015-11-01 00:00:00pages
2139-45issue
11eissn
0141-5492issn
1573-6776pii
10.1007/s10529-015-1907-0journal_volume
37pub_type
杂志文章,评审abstract::A coupled in silico thermodynamic and probabilistic metabolic control analysis methodology was verified by applying it to the glycerol biosynthetic pathway in Saccharomyces cerevisiae. The methodology allows predictions even when detailed knowledge of the enzyme kinetics is lacking. In a metabolic steady state, we fou...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1675-2
更新日期:2015-02-01 00:00:00
abstract::In the industrial production of bioethanol from lignocellulosic biomass, a strain of Saccharomyces cerevisiae that can ferment xylose in the presence of inhibitors is of utmost importance. The recombinant, industrial-flocculating S. cerevisiae strain NAPX37, which can ferment xylose, was used as the parent to delete t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1581-7
更新日期:2014-10-01 00:00:00
abstract:OBJECTIVES:For the stress from fermenters, downstream processing equipment, and wastewater treatment to be alleviated, lowering salt-dependence in the ectoine synthesis process is of great significance in the moderately halotolerant Halomonas hydrothermalis Y2. RESULTS:In H. hydrothermalis Y2, the σ70- and σ38-control...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-021-03084-3
更新日期:2021-01-27 00:00:00
abstract::An automated sequential injection analysis (SIA) system using stop-flow technique was developed to determine methanol concentration by means of the enzymatic reactions of alcohol oxidase and peroxidase. Its application as an on-line device for monitoring Pichia pastoris fermentations producing an heterologous protein ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1026228518418
更新日期:2003-11-01 00:00:00
abstract::N(omega)-2,2,4,6,7-Pentamethyldihydrobenzofuran-5-sulfonyl (N(omega)-Pbf)-protected oligoarginine was directly conjugated to poly(amino acid) derivatives modified with a long alkyl chain. The final concentration of conjugated peptides was easily controlled by the feed ratio of oligoarginine to polymer backbone and a f...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-7838-4
更新日期:2005-07-01 00:00:00
abstract:OBJECTIVES:We examined the importance of aptamer usage under the same condition as the selection process by employing the previously selected aptamers for calmodulin (CaM) which includes a non-natural fluorogenic amino acid, 7-nitro-2,1,3-benzoxadiazole. RESULTS:We added five amino acids at the N-terminus which was em...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2257-2
更新日期:2017-03-01 00:00:00
abstract::The shape of the hydrophobic tunnel leading to the active site of Penicillium expansum lipase (PEL) was redesigned by single-point mutations, in order to better understand enzyme enantioselectivity towards naproxen. A variant with a valine-to-glycine substitution at residue 237 exhibited almost no enantioselectivity (...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1405-1
更新日期:2014-03-01 00:00:00
abstract::Neural cortical cells, isolated from prenatal rat cerebra, were grown on surface-modified poly(lactic-co-glycolic acid, 65:35) (PLGA) films coated with poly-D: -lysine (PDL) with either laminin (LN), fibronectin (FN) or collagen (CN). Immunocytochemistry showed that the isolated cells were highly immunopositive for bo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-6585-2
更新日期:2005-01-01 00:00:00
abstract::Biodesulfurization (BDS) in a bioreactor packed with a catalytic bed of silica containing immobilized Rhodococcus rhodochrous was studied. Various bed lengths and support particle sizes were evaluated for BDS of dibenzothiophene (DBT) and gas oil. The sulfur-containing substrates were introduced separately into the bi...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1529-y
更新日期:2014-08-01 00:00:00
abstract::A brief historical perspective on metagenomics is given followed by a discussion of the rapid progress in this field largely defined by transition to the next generation sequencing technologies. Problems and challenges connected to this transition are also addressed. The review focuses on recent literature describing ...
journal_title:Biotechnology letters
pub_type: 历史文章,杂志文章,评审
doi:10.1007/s10529-010-0306-9
更新日期:2010-10-01 00:00:00
abstract:OBJECTIVE:To examine the role of miR-124a in LPS-induced septic cardiac insufficiency where underlying mechanism is unclear. RESULTS:Expression of miR-124a was decreased in myocardium of LPS-induced septic cardiac dysfunction model. miR-124a antagomiR or agomiR were injected via tail vein to induce miR-124a-dysregulat...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2368-4
更新日期:2017-09-01 00:00:00
abstract::Brevibacterium epidermis DSM 20659 is a halotolerant Gram-positive bacterium which can synthesize the osmolyte, ectoine, but prefers to take it up from its environment. The present study revealed that B. epidermis is equipped with at least one transport system for ectoine, with a maximal transport velocity of 15.7 +/-...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9149-9
更新日期:2006-11-01 00:00:00
abstract::The extracellular domains (ECD) of epidermal growth factor receptors, ErbB1, 2, 3 and 4, were designed as soluble dimeric forms. Each ECD was fused to a short hinge region derived from IgG, such that the stable dimer could be formed with disulfide bridges. This hinge-tagged design minimized the molecular weight to app...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0160-9
更新日期:2010-03-01 00:00:00
abstract::Genetically-coded, fluorescence resonance energy transfer (FRET) biosensors are widely used to study molecular events from single cells to whole organisms. They are unique among biosensors because of their spontaneous fluorescence and targeting specificity to both organelles and tissues. In this review, we discuss the...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-006-9193-5
更新日期:2006-12-01 00:00:00
abstract::Biotransformation of 1,3-dichloro-2-propanol (DCP) to epichlorohydrin (ECH) by the whole cells of recombinant Escherichia coli expressing halohydrin dehalogenase was limited by product inhibition. To solve this problem and improve the ECH yield, a biotransformation strategy using resin-based in situ product removal (I...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1165-y
更新日期:2013-06-01 00:00:00
abstract::Monascus aurantiacus produces high amounts of citrinin which is a mycotoxin with nephrotoxic activity. Six putative citrinin biosynthesis genes have been discovered in M. purpureus and at least 10 genes are responsible for its biosynthesis. However, the sequence of citrinin pathway gene cluster in M. aurantiacus has n...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0745-y
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVE:The effects of a brief (3 days) and prolonged (6 days) period of incubation in darkness and light on the biomass content, lipid content and fatty acid profile in Chlorella vulgaris UMT-M1 were determined. RESULTS:Three days of incubation in darkness increased saturated fatty acid (SFA) content from 34.0 to 4...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-021-03077-2
更新日期:2021-01-12 00:00:00
abstract::1,3-Propanediol production by Klebsiella pneumoniae was studied in batch cultures under N2 flow and four airflow systems. Different byproducts were formed under different aeration conditions. An anaerobic/aerobic combined fed-batch culture was developed giving 70 g 1,3-propanediol l(-1) and 16 g 2,3-butanediol l(-1) w...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000025902.44458.f4
更新日期:2004-06-01 00:00:00
abstract:OBJECTIVES:Developing an effective HIV vaccine that stimulates the humoral and cellular immune responses is still challenging because of the diversity of HIV-1 virus, polymorphism of human HLA and lack of a suitable delivery system. RESULTS:Using bioinformatics tools, we designed a DNA construct encoding multiple epit...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-019-02734-x
更新日期:2019-11-01 00:00:00
abstract::The inhibition of protein-tyrosine phosphatase 1B (PTP1B) is a potential target for treatment of type 2 diabetes. Vanadium and zinc metal coordinated complexes have insulin-enhancing activities, and while vanadium compounds inhibit PTP1B, little is known on the mode of action of zinc compounds. In this study we develo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-7855-8
更新日期:2005-02-01 00:00:00
abstract::In order to treat the kitchen wastes and produce hydrogen, anaerobic fermentation technology was used in this experiment. The results showed that the fermentation type changed from mixed acid fermentation to ethanol fermentation in a continuous stirred tank reactor (CSTR) 22 days after start-up. The maximum efficiency...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0024-3
更新日期:2009-09-01 00:00:00
abstract::A microtiter plate-based assay was developed to evaluate the ability of lipases to perform transesterifications when employed in different organic solvents. A 4-nitrophenol assay was carried out employing seven different lipase formulations and two fatty acid methyl esters with different chain lengths in a total of si...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1721-0
更新日期:2015-03-01 00:00:00
abstract::Extracts of Salvia species are used in traditional medicine to treat various diseases. The economic importance of this genus has increased in recent years due to evidence that some of its secondary metabolites have valuable pharmaceutical and nutraceutical properties.The bioactivity of sage extracts is mainly due to t...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-013-1350-z
更新日期:2014-02-01 00:00:00
abstract::Electroporation of Litopenaeus schmitti embryos was used to transfer the pE300tiGH15 plasmid that contains the tilapia growth hormone gene (tiGH) complexed with a nuclear localization signal peptide into the zygotes. The gene construct was detected in 35 (36%) of the 98 larvae screened by PCR and Southern blot analyse...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9636-2
更新日期:2008-05-01 00:00:00
abstract::Anthocyanic vacuolar inclusions (AVIs) appear as dark red-to-purple spheres of various sizes in vacuoles of grapevine (Vitis vinifera L.) cell suspension culture due to their interaction with anthocyanins. AVIs were purified and the bound anthocyanins extracted and analysed by HPLC from two lines of V. vinifera isolat...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024028603089
更新日期:2003-06-01 00:00:00
abstract:OBJECTIVES:A potent HIV vaccine should overcome some limitations such as polymorphism of human HLA, the diversity of HIV-1 virus, and the lack of an effective delivery system. In this study, a DNA construct encoding Nef60-84, Nef126-144, Vpr34-47, Vpr60-75, Gp16030-53, Gp160308-323, and P248-151 epitopes was designed u...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03060-3
更新日期:2021-01-01 00:00:00
abstract::Human granulocyte-macrophage colony stimulating factor (hGM-CSF) is used clinically to treat leucopenia typically caused by cancer chemotherapy or radiotherapy. This study used multiple strategies to obtain very high expression levels of OsrhGM-CSF (14 microg/seed) in rice endosperm. Electron micrographs of immunogold...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9717-2
更新日期:2008-09-01 00:00:00
abstract::Ramoplanin is a lipopeptide antibiotic active against multi-drug-resistant, Gram-positive pathogens. Structurally, it contains a di-mannose moiety attached to the peptide core at Hpg(11). The biosynthetic gene cluster of ramoplanin has already been reported and the assembly of the depsipeptide has been elucidated but ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1233-3
更新日期:2013-09-01 00:00:00
abstract::In batch culture for glutathione production with Candida utilis, a higher temperature (30 degrees C) was required to hasten cell growth while a lower temperature (26 degrees C) was needed to increase the production of glutathione. A two-stage temperature control strategy was used to enhance both the yield and the prod...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024034508136
更新日期:2003-06-01 00:00:00
abstract::At the core of a biomanufacturing process for recombinant proteins is the production cell line. It influences the productivity and product quality. Its characteristics also dictate process development, as the process is optimized to complement the producing cell to achieve the target productivity and quality. Advances...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-015-1843-z
更新日期:2015-08-01 00:00:00