Abstract：
:Monascus aurantiacus produces high amounts of citrinin which is a mycotoxin with nephrotoxic activity. Six putative citrinin biosynthesis genes have been discovered in M. purpureus and at least 10 genes are responsible for its biosynthesis. However, the sequence of citrinin pathway gene cluster in M. aurantiacus has not been reported. Here, the putative sequence of citrinin biosynthetic gene cluster was obtained by a PCR-based strategy for screening a genome fosmid library of M. aurantiacus. A sequence of 43 kb revealed 16 ORFs including the six putative biosynthetic genes reported previous. The putative gene cluster consists of a polytekide synthetase encoding one PKS module, an oxidoreductase gene, three dehydrogenase genes, an acyl-coenzyme A synthetase gene, a membrane transport protein gene, a transcriptional activator gene as well as genes encoding proteins of undefined function.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Li YP,Xu Y,Huang ZBdoi
10.1007/s10529-011-0745-ysubject
Has Abstractpub_date
2012-01-01 00:00:00pages
131-6issue
1eissn
0141-5492issn
1573-6776journal_volume
34pub_type
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journal_title：Biotechnology letters
pub_type： 杂志文章
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journal_title：Biotechnology letters
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doi：10.1007/s10529-006-0003-x
更新日期：2006-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：2004-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s10529-015-1786-4
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journal_title：Biotechnology letters
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pub_type： 杂志文章
doi：10.1007/s10529-007-9403-9
更新日期：2007-09-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 历史文章,杂志文章,评审
doi：10.1007/s10529-010-0306-9
更新日期：2010-10-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-007-9532-1
更新日期：2008-02-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-006-9146-z
更新日期：2006-11-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-008-9841-z
更新日期：2009-01-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-004-7855-8
更新日期：2005-02-01 00:00:00
abstract：:An optimal pooling system, called Accurate and Fast Target Screening, has been developed for high-throughput identifying the rare marker-free transformants. This system can identify targets between 10- and 100-fold more efficiently than analysis of individual samples. By calculating the efficiency for different propor...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-006-9129-0
更新日期：2006-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s10529-008-9634-4
更新日期：2008-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s10529-013-1195-5
更新日期：2013-08-01 00:00:00
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更新日期：2015-08-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-007-9561-9
更新日期：2008-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s10529-016-2051-1
更新日期：2016-05-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1023/a:1026281627466
更新日期：2003-11-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-007-9583-3
更新日期：2008-04-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-007-9496-1
更新日期：2008-01-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-005-5718-6
更新日期：2006-03-01 00:00:00
abstract：:An endo-beta-(1-->3),(1-->4)-glucanase gene (bglBC1) from Bacillus circulans ATCC21367 was modified by substituting its native promoter with a strong promoter, BJ27X, to increase expression of the gene when cloned into B. subtilis RM125 and B. megaterium ATCC14945. A 771-bp endo-beta-(1-->3),(1-->4)-glucanase open rea...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-004-4581-1
更新日期：2004-11-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-009-0060-z
更新日期：2009-11-01 00:00:00
abstract：:A 4 kb BamHI-HindIlI fragment, corresponding to the cry2A operon of Bacillus thuringiensis subsp. kurstaki strain BNS3, was cloned. The sequencing of the corresponding cry2Aa-type gene, termed crybns3-4, revealed an open reading frame of 1902 bp, encoding a protein of 633 amino-acid residues. Both nucleotide and amino...
journal_title：Biotechnology letters
pub_type： 杂志文章
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更新日期：2003-08-01 00:00:00
