Abstract:
:A new full-length beta-1,3-glucanase cDNA, MpGlu, was isolated from a plantain (Musa paradisica) by the rapid amplification of cDNA ends (RACE) technique. Recombinant GST-MpGlu protein, expressed in E. coli, hydrolyzed (1-->3),(1-->6)-beta-glucan of Laminaria digitata and inhibited the growth of Fusarium oxysporum fsp. cubense (race 4) suggesting that it is a beta-1,3-glucanase. Southern blot analysis indicated that there is one copy of MpGlu in the plantain genome. MpGlu gene expression was detected in plantain leaves, peel, and pulp by RT-PCR. Northern blot analysis revealed that the expression of MpGlu was up-regulated by Fusarium infection. Subcellular localization analysis indicated that 28 residues at the N-terminal end are necessary for extracellular secretion, while 32 residues at the C-terminal end are necessary to target the protein into vacuoles.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Jin X,Feng D,Wang H,Wang Jdoi
10.1007/s10529-007-9403-9subject
Has Abstractpub_date
2007-09-01 00:00:00pages
1431-7issue
9eissn
0141-5492issn
1573-6776journal_volume
29pub_type
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