Inhibition of berberine hydrochloride on Candida albicans biofilm formation.

abstract：:An endo-beta-(1-->3),(1-->4)-glucanase gene (bglBC1) from Bacillus circulans ATCC21367 was modified by substituting its native promoter with a strong promoter, BJ27X, to increase expression of the gene when cloned into B. subtilis RM125 and B. megaterium ATCC14945. A 771-bp endo-beta-(1-->3),(1-->4)-glucanase open rea...

journal_title：Biotechnology letters

authors： Kim KH,Kim YO,Ko BS,Youn HJ,Lee DS

更新日期：2004-11-01 00:00:00

abstract：OBJECTIVES:Breast cancer is a popular fatal malignant tumor for women with high of rates incidence and mortality. Development of the new approaches for breast cancer targeted diagnosis and chemotherapy is emergently needed by the current clinical practice, the important first step is finding a breast cancer specificall...

journal_title：Biotechnology letters

authors： Jin H,Gao X,Xiao L,He H,Cheng S,Zhang C,Hou Y,Song F,Su X,Gao Q,Lu Z,Yang R,Song X,Yang J,Duan W,Hou Y

更新日期：2021-01-01 00:00:00

abstract：:Quercetin was methylated with two O-methyltransferases (OMTs) expressed in E. coli. A construct (RSOMT) was designed to express two OMTs: ROMT-9, which methylates specifically at the 3'-hydroxyl group of quercetin and SOMT-2, which methylates at the 4'-hydroxyl group. Both OMT genes were driven by T7 promoters and had...

journal_title：Biotechnology letters

authors： Kim BG,Shin KH,Lee Y,Hur HG,Lim Y,Ahn JH

更新日期：2005-12-01 00:00:00

abstract：OBJECTIVE:The dye-decolorizing peroxidase from Bacillus amyloliquefaciens, BaDyP, was identified to be an efficient catalyst for the degradation of phenolic β-ether lignin model dimer guaiacylglycerol-β-guaiacyl ether (GGE) and dyes. RESULTS:Efeb gene encoding BaDyP from B. amyloliquefaciens MN-13 consisted of 1257 bp...

journal_title：Biotechnology letters

authors： Yang J,Gao T,Zhang Y,Wang S,Li H,Li S,Wang S

更新日期：2019-09-01 00:00:00

abstract：:The addresses given for the authors of this paper were incorrect. The correct assignments of the authors are given in this erratum. ...

journal_title：Biotechnology letters

authors： Li W,Chen Y,Sheng Y,Xie Z,Tang Y

更新日期：2018-01-01 00:00:00

abstract：OBJECTIVES:An easy-to-operate method of using R-ω-transaminase has been developed by fusing it to an elastin-like polypeptide and forming a complex with D-amino acid oxidase. RESULTS:R-ω-Transaminase (R-ω-TA) was fused to an elastin-like polypeptide (ELP) through genetic engineering of the enzyme. The enzyme was purif...

journal_title：Biotechnology letters

authors： Gao Q,Fu Y,Peng Y,Liu W,Feng W

更新日期：2016-03-01 00:00:00

abstract：:The developmental ability among embryos produced by three different techniques were examined: there were no significant differences in the developmental rate in porcine embryos produced by in vitro fertilization (IVF) and first generation of somatic cell nucleus transfer (SCNT), but the developmental rate dropped shar...

journal_title：Biotechnology letters

authors： Huang Y,Xie W,Yao C,Han Y,Tan G,Zhou Y,Zhu J,Pang D,Li Z,Tang X

更新日期：2014-06-01 00:00:00

abstract：:Microbial solar biofuels offer great promise for future sustainable food, fuels and chemicals but are limited by low productivities and a requirement for large land areas to harvest sunlight. A 71 % increase in combined photosynthetic activity was achieved by illuminating both Rhodobacter sphaeroides and Arthrospira (...

journal_title：Biotechnology letters

authors： Redwood MD,Dhillon R,Orozco RL,Zhang X,Binks DJ,Dickinson M,Macaskie LE

更新日期：2012-12-01 00:00:00

abstract：:A very high gravity (VHG) repeated-batch fermentation system using an industrial strain of Saccharomyces cerevisiae PE-2 (isolated from sugarcane-to-ethanol distillery in Brazil) and mimicking industrially relevant conditions (high inoculation rates and low O(2) availability) was successfully operated during fifteen c...

journal_title：Biotechnology letters

authors： Pereira FB,Gomes DG,Guimarães PM,Teixeira JA,Domingues L

更新日期：2012-01-01 00:00:00

abstract：:Previously, we constructed a genetically modified luciferase of Photinus pyralis that generates more than 10-fold higher luminescence intensity than the wild-type enzyme. In this study, we demonstrate that this modified luciferase enables us to detect ATP at 10(-18) mol, which is almost equal to the quantity contained...

journal_title：Biotechnology letters

authors： Noda K,Matsuno T,Fujii H,Kogure T,Urata M,Asami Y,Kuroda A

更新日期：2008-06-01 00:00:00

abstract：:A new technique for the direct production of recombinant baculovirus in the silkworm larvae is described. To assess the utility of this method, a combination of Bombyx mori nucleopolyhedroviral genome, transfer vector and Lipofectin was co-injected directly into newly ecdysed fifth instar, silkworm larvae. The recombi...

journal_title：Biotechnology letters

authors： Lee KS,Kim BY,Je YH,Woo SD,Sohn HD,Jin BR

更新日期：2007-01-01 00:00:00

abstract：OBJECTIVES:To improve heterologous proteins production, we constructed a maltose-inducible expression system in Bacillus subtilis. RESULTS:An expression system based on the promoter for maltose utilization constructed in B. subtilis. Successively, to improve the performance of the P malA -derived system, mutagenesis w...

journal_title：Biotechnology letters

authors： Yue J,Fu G,Zhang D,Wen J

更新日期：2017-08-01 00:00:00

abstract：OBJECTIVE:To construct a highly expressed and active MEK1R4F (a constitutively active form of mitogen-activated protein kinase kinase 1) by fusion of soluble adenylate kinase (Adk) tag, resulting in Adk-MEK1R4F protein suitable for preparation of phosphorylated ERK. RESULTS:We fused the Adk to the N-terminus of MEK1R4...

journal_title：Biotechnology letters

authors： Yang F,Li H

更新日期：2017-10-01 00:00:00

abstract：:Gluconobacter oxydans LMG 1489 was selected as the best strain for NAD(P)-dependent polyol dehydrogenase production. The highest enzyme activities were obtained when this strain was cultivated on a medium consisting of 30 g glycerol l(-1), 7.2 g peptone l(-1) and 1.8 g yeast extract l(-1). Two D-fructose reducing, NAD...

journal_title：Biotechnology letters

authors： Parmentier S,Beauprez J,Arnaut F,Soetaert W,Vandamme EJ

更新日期：2005-03-01 00:00:00

abstract：:The expression of secreted proteins in filamentous fungi is down-regulated by a transcriptional feedback mechanism under endoplasmic reticulum stress, termed repression under secretion stress (RESS). To investigate the RESS mechanism, we analyzed the expression of the Taka-amylase A gene (amyB) in Aspergillus oryzae, ...

journal_title：Biotechnology letters

authors： Zhou B,Wang C,Wang B,Li X,Xiao J,Pan L

更新日期：2015-02-01 00:00:00

abstract：:miR-21 is highly expressed in a variety of cancers, suggesting that it might play a role in the process of oncogenesis, as supported by it directly causing pre-B cell lymphomas in transgenic mice. Rm21LG transgenic mice for the conditional co-expression of miR-21 and luciferase (Luc) mediated by Cre/lox P system were ...

journal_title：Biotechnology letters

authors： Du T,Jia J,Lin X,Xie R,Li J,Xiao D,Xu K

更新日期：2014-01-01 00:00:00

abstract：:The Rho1 protein is a GTPase that participates in cell wall biogenesis. We analyzed the transcript levels of laccase genes (lccl, lcc2, lcc3, lcc4, lcc5, and lcc9), and a xylanase gene (xyl3) in Fusarium oxysporum f. sp. lycopersici strain 4287 (wild type) and two mutant strains; rhol::hyg that lacks a functional Rho1...

journal_title：Biotechnology letters

authors： Reyes-Medina MA,Macías-Sánchez KL

更新日期：2015-03-01 00:00:00

abstract：:Streptomyces is an attractive host for heterologous protein secretion. To further optimize its expression capacity, better expression vectors will be helpful. Here, based on pSGL1, a high copy number plasmid present in Streptomyces globisporus C-1027, we constructed a series of novel E. coli-Streptomyces shuttle expre...

journal_title：Biotechnology letters

authors： Zhu Y,Wang L,Du Y,Wang S,Yu T,Hong B

更新日期：2011-02-01 00:00:00

abstract：:An open reading frame (ORF) encoding chitin oligosaccharide deacetylase (Pa-COD) gene and its signal sequence was cloned from the Vibrio parahaemolyticus KN1699 genome and its sequence was analyzed. The ORF encoded a 427 amino acid protein, including the 22 amino acid signal sequence. The deduced amino acid sequence w...

journal_title：Biotechnology letters

authors： Kadokura K,Sakamoto Y,Saito K,Ikegami T,Hirano T,Hakamata W,Oku T,Nishio T

更新日期：2007-08-01 00:00:00

abstract：:Three chloroplast genes, chlL, chlN and chlB, encoding the light-independent protochlorophyllide oxidoreductase (LIPOR) in Chlorella protothecoides CS-41 growing either photoautotrophically, mixotrophically or heterotrophically, were all transcribed constitutively independent of illumination and presence of glucose. S...

journal_title：Biotechnology letters

authors： Shi C,Shi X

更新日期：2006-02-01 00:00:00

abstract：:We truncated the VP2 protein of infectious bursal disease virus into five fragments: V1-5. All fragments were displayed on the inner membrane of the Escherichia coli periplasm. After disruption of the outer membrane, spheroplasts that had anchored with the VP2 fragment were incubated with an anti-VP2 polyclonal antibo...

journal_title：Biotechnology letters

authors： Guo M,Xu LM,Zhou B,Yin JC,Ye XL,Ren GP,Li DS

更新日期：2014-03-01 00:00:00

abstract：:A polysaccharide from the traditional Chinese medicinal herb, Bletilla striata (Thunb.) Reichb. f., was isolated, purified and characterized. It induced the proliferation of human umbilical vascular endothelial cells and the expression of vascular endothelial growth factor up to 156% and 147% of control after 72 h, re...

journal_title：Biotechnology letters

authors： Wang C,Sun J,Luo Y,Xue W,Diao H,Dong L,Chen J,Zhang J

更新日期：2006-04-01 00:00:00

abstract：OBJECTIVES:Establish a method to indirectly measure evaporation in microwell-based cell culture systems and show that the proposed method allows compensating for liquid losses in fed-batch processes. RESULTS:A correlation between evaporation and the concentration of Na+ was found (R2 = 0.95) when using the 24-well-bas...

journal_title：Biotechnology letters

authors： Wiegmann V,Martinez CB,Baganz F

更新日期：2018-07-01 00:00:00

abstract：:Fungal diseases damage crop plants and affect agricultural production. Transgenic plants have been produced by inserting antifungal genes to confer resistance against fungal pathogens. Genes of fungal cell wall-degrading enzymes, such as chitinase and glucanase, are frequently used to produce fungal-resistant transgen...

journal_title：Biotechnology letters

authors： Ceasar SA,Ignacimuthu S

更新日期：2012-06-01 00:00:00

abstract：:The aim of this study was to characterize the growth of the fungus Leucoagaricus gongylophorus LEU18496, isolated from the fungus garden of the nest of leaf cutter ants Atta mexicana. The fungus garden was cultivated in an artificial laboratory nest and the fungus further grown in submerged (SmC) and solid state (SSC)...

journal_title：Biotechnology letters

authors： Maya-Yescas ME,Revah S,Le Borgne S,Valenzuela J,Palacios-González E,Terrés-Rojas E,Vigueras-Ramírez G

更新日期：2021-01-02 00:00:00

abstract：:Bacterial and fungal contaminants of enset (Ensete ventricosum) cultures and microbes associated with surface-sterilized field material were identified by 16S/26S rDNA sequencing. Ten bacterial species were identified in 16 isolates from in vitro cultures and seven in 10 isolates from field clones. Three yeast species...

journal_title：Biotechnology letters

authors： Birmeta G,Passoth V,Roos S,Welander M

更新日期：2004-12-01 00:00:00

abstract：:For most cultivated mammalian cells, glutamine is an essential medium component. However, glutamine consumption results in the production of ammonia, a cytotoxic byproduct. Here we investigated the effect of glutamine reduction on recombinant protein production and ammonia accumulation in transiently transfected CHO a...

journal_title：Biotechnology letters

authors： Rajendra Y,Kiseljak D,Baldi L,Hacker DL,Wurm FM

更新日期：2012-04-01 00:00:00

abstract：:Solubilization of lignin and carbohydrates from the lignin-holocellulose structure of cotton seed-coat fragments was investigated by UV/VIS spectrometry. Xylanase (Pulpzyme HC) pre-treatment partially destroyed the lignocellulosic structure of the seed-coat fragments, producing reducing sugars and soluble lignin in th...

journal_title：Biotechnology letters

authors： Csiszár E,Losonczi A,Koczka B,Szakács G,Pomlényi A

更新日期：2006-05-01 00:00:00

abstract：:Methods for the preparation of carrier-free insoluble enzymes are reviewed. The technology of cross-linked enzyme aggregates has now been applied to a range of synthetically useful activities. Fusion proteins are also gaining momentum because they allow a relatively selective aggregation or even a specific self-assemb...

journal_title：Biotechnology letters

authors： Roessl U,Nahálka J,Nidetzky B

更新日期：2010-03-01 00:00:00

abstract：:A brief historical perspective on metagenomics is given followed by a discussion of the rapid progress in this field largely defined by transition to the next generation sequencing technologies. Problems and challenges connected to this transition are also addressed. The review focuses on recent literature describing ...

journal_title：Biotechnology letters

authors： Chistoserdova L

更新日期：2010-10-01 00:00:00