Abstract:
OBJECTIVES:Establish a method to indirectly measure evaporation in microwell-based cell culture systems and show that the proposed method allows compensating for liquid losses in fed-batch processes. RESULTS:A correlation between evaporation and the concentration of Na+ was found (R2 = 0.95) when using the 24-well-based miniature bioreactor system (micro-Matrix) for a batch culture with GS-CHO. Based on these results, a method was developed to counteract evaporation with periodic water additions based on measurements of the Na+ concentration. Implementation of this method resulted in a reduction of the relative liquid loss after 15 days of a fed-batch cultivation from 36.7 ± 6.7% without volume corrections to 6.9 ± 6.5% with volume corrections. CONCLUSION:A procedure was established to indirectly measure evaporation through a correlation with the level of Na+ ions in solution and deriving a simple formula to account for liquid losses.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Wiegmann V,Martinez CB,Baganz Fdoi
10.1007/s10529-018-2556-xsubject
Has Abstractpub_date
2018-07-01 00:00:00pages
1029-1036issue
7eissn
0141-5492issn
1573-6776pii
10.1007/s10529-018-2556-xjournal_volume
40pub_type
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1501-x
更新日期:2014-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2016-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
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更新日期:2006-09-01 00:00:00
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