Abstract:
:Bioreactors are essential in tissue engineering, not only because they provide an in vitro environment mimicking in vivo conditions for the growth of tissue substitutes, but also because they enable systematic studies of the responses of living tissues to various mechanical and biochemical cues. The basic principles of bioreactor design are reviewed, the bioreactors commonly used for the tissue engineering of cartilage, bone and cardiovascular systems are assessed in terms of their performance and usefulness. Several novel bioreactor types are also reviewed.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Chen HC,Hu YCdoi
10.1007/s10529-006-9111-xsubject
Has Abstractpub_date
2006-09-01 00:00:00pages
1415-23issue
18eissn
0141-5492issn
1573-6776journal_volume
28pub_type
杂志文章,评审abstract::Genes coding for Vitreoscilla hemoglobin (VHb) with peroxisome targeting signal (PTS1) tag and beta-galactosidase were co-expressed in Pichia pastoris under the alcohol oxidase1 (AOX1) promoter. The expression of VHb-PTS1 had no positive effect on cell growth but significantly enhanced the whole cell beta-galactosidas...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025072020791
更新日期:2003-09-01 00:00:00
abstract::Error-prone PCR was used to create more thermoactive and/or thermostable variants of thermoalkalophilic lipases. A variant of the α6 helix (lid domain), with an 189E to V substitution at residue 189, lost its thermostability but exhibited higher activity than that of its wild-type predecessor (r03Lip). Site-saturation...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0635-3
更新日期:2011-09-01 00:00:00
abstract:OBJECTIVES:To find new metabolic engineering strategies to improve the yield of acetone in Escherichia coli. RESULTS:Results of flux balance analysis from a modified Escherichia coli genome-scale metabolic network suggested that the introduction of a non-oxidative glycolysis (NOG) pathway would improve the theoretical...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2115-2
更新日期:2016-08-01 00:00:00
abstract::Virus-like particles (VLPs) are a promising and developing option for vaccination and gene therapy. They are also interesting as shuttles for drug targeting. Currently, several different gene expression systems are available, among which the silkworm expression system is known for its mass production capacity. However...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-018-2516-5
更新日期:2018-04-01 00:00:00
abstract::The use of microRNAs (miRNAs) for improving the efficiency of recombinant protein production by CHO cells is gaining considerable interest for their ability to regulate entire molecular networks. Differential miRNA expression profiling and large-scale transient screening have been the prerequisite for the selection of...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1751-7
更新日期:2015-04-01 00:00:00
abstract::The maltose-binding protein (MBP), which possesses a large number of exposed hydrophobic zones, can be used as a link for the immobilization of growth factors. The amount of immobilized MBP-vascular endothelial growth factors (VEGFs) for polystyrene surface was increased with respect to increasing protein, showing 101...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0060-z
更新日期:2009-11-01 00:00:00
abstract::Phanerochaete chrysoporium morphology and manganese peroxidase (MnP) productivity was characterised in a scalable, modularised 1145 cm(3) membrane gradostat reactor in response to switching between an enhanced production medium and a nutrient limited feed (50% C and N reduction). Irrespective of the feed composition u...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0443-1
更新日期:2011-02-01 00:00:00
abstract::The lipase-catalyzed reaction is useful to obtain sugar esters with chemically defined structures and will contribute to the synthesis of sugar-based compounds by a chemo-enzymatic pathway. The synthesis of sugar esters in nonaqueous media has been attempted for a quarter century. To facilitate the reactions, they hav...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-011-0663-z
更新日期:2011-10-01 00:00:00
abstract::Cotton fabric samples were treated with a formulation of a total crude Trichoderma reesei cellulase in a two-step procedure. In the first step, samples were treated at a low liquor ratio by padding through the enzyme formulation at 21 degrees C and 55 degrees C with a wet pickup of 100% and batched for 12 h. The sampl...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9569-1
更新日期:2008-03-01 00:00:00
abstract::Using the optimal concentrations of octanoic acid (0.75 mM) and ethyl mercaptan (2 mM), as the most effective sulfur donor, Pseudomonas reptilivora produced 74 microg lipoic acid per dry cell weight at pH 7.5 and 30 degrees C in a fermenter over 9 h. The dry cell weight was 13.9 g l(-1). ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9769-3
更新日期:2008-10-01 00:00:00
abstract::Microgravity can influence cell growth and function. A transfected Sp2/0 myeloma cell line P3A2 producing a human IgG1 anti-TNFa monoclonal antibody was cultivated in static culture, spinner flasks and simulated microgravity using a rotating wall vessel bioreactor. Microgravity significantly decreased cell growth (fro...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025003800414
更新日期:2003-08-01 00:00:00
abstract::Crude rapeseed oil and post-refining fatty acids were used as substrates for oxalic acid production by a mutant of Aspergillus niger. Both the final concentration and the yield of the product were highest at pH 4 to 5. With a medium containing 50 g lipids l(-1), production reached a maximum of 68 g oxalic acid l(-1) a...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024082130677
更新日期:2003-06-01 00:00:00
abstract::Recombinant Mycobacterium sp. strain MR65 carrying dszABCD genes was used for desulfurization of 10-methylbenzo[b]naphtho[2,1-d]thiophene (10-methyl BNT) in the hexadecane phase. The specific activity was 25% of that of dibenzothiophene (DBT). One of two major metabolites of 10-methyl BNT produced by strain MR65 was i...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1023584805911
更新日期:2003-05-01 00:00:00
abstract::The protective effects of polymer additives, including a group of viscosity-enhancing polymer poly-γ-glutamic acid (γPGA; 10, 50, and 500 kDa) and surface-active polymer Pluronic F68, on Chinese hamster ovary cells against damage due to shear stress were investigated in shake-flask cultures. The level of protection wa...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0982-8
更新日期:2012-10-01 00:00:00
abstract::Electroporation of Litopenaeus schmitti embryos was used to transfer the pE300tiGH15 plasmid that contains the tilapia growth hormone gene (tiGH) complexed with a nuclear localization signal peptide into the zygotes. The gene construct was detected in 35 (36%) of the 98 larvae screened by PCR and Southern blot analyse...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9636-2
更新日期:2008-05-01 00:00:00
abstract::Pancreatic islet transplantation is used to treat diabetes mellitus that has minimal complications and avoids hypoglycemic shock. Conformal microencapsulation of pancreatic islets improves their function by blocking immunogenic molecules while protecting fragile islets. However, production of empty alginate capsules d...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1300-9
更新日期:2013-12-01 00:00:00
abstract::A novel and simple method for detection of mutations in DNA oligonucleotides using a double-stranded DNA specific dye (SYBR Green I) is reported. The SYBR Green I is bound specifically with a duplex DNA oligonucleotide (it tercalation). This intercalation induces fluorescent emission at 525 nm with excitation at 494 n...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025661730518
更新日期:2003-10-01 00:00:00
abstract:OBJECTIVES:To uncover key genes and pathways regulated by SCL3, a GRAS transcription factor, in the context of gibberellin (GA) in the roots of the model plant Arabidopsis thaliana. RESULTS:Gene expression profiles of ga1-3 mutant and ga1-3 and scl3 double mutant are considerably similar to each other, revealed by Pri...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-02850-z
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVES:To study the roles and mechanisms of HuR in cancer stem cell maintenance of lung cancer. RESULTS:HuR expression was increased in tumor spheres of lung cancer cells. Knockdown of HuR suppressed spheroid formation and size, inhibited the expression of stemness-related marker, Oct4, Nanog and ALDH in lung canc...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2512-9
更新日期:2018-04-01 00:00:00
abstract::A successful nerve regeneration process was achieved with nerve repair tubes made up of 1-ethyl-3(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) cross-linked carboxymethyl chitosan (CM-chitosan) with improved biodegradability. Chitosan has a very slow degradation rate, while the EDC cross-linked CM-chitosan t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0123-1
更新日期:2010-01-01 00:00:00
abstract::The gene, xynB, from Streptomyces olivaceoviridis A1 encoding xylanase, XYNB, with a high specific activity for xylan, was transformed into potato (Solanum tuberosum L.) by Agrobacterium tumefaciens. The integration of xynB into genomic DNA was confirmed by PCR and reverse transcriptase-PCR. The gene was expressed und...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9280-7
更新日期:2007-04-01 00:00:00
abstract::Glycine soja is a species of soybean that survives in adverse environments including high salt and drought conditions. We constructed a cDNA library from G. soja seedlings treated with NaCl and isolated a glutathione S-transferase gene (GsGST: GQ265911) from the library. The cDNA encoding GsGST contains an open readin...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0269-x
更新日期:2010-08-01 00:00:00
abstract::The profiles of carotenoids and production of β-carotene by six eustigmatophytes, Eustigmatos magnus, Eustigmatos polyphem, Eustigmatos vischeri, Vischeria helvetica, Vischeria punctata and Vischeria stellata, grown in a bubble column photobioreactor were measured. All eustigmatophytes contained β-carotene, violaxanth...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0996-2
更新日期:2012-11-01 00:00:00
abstract::Acetic acid is by-product from fermentation processes for producing succinic acid using Mannheimia succiniciproducens . To obtain pure succinic acid from the final fermentation broth, acetic acid was selectively removed based on the different extractability of succinic acid and acetic acid with pH using tri-n-octylami...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/B:BILE.0000045656.00138.93
更新日期:2004-10-01 00:00:00
abstract:OBJECTIVES:To provide a simple method to make a stable ΔF508-CFTR-expressing T84 cell line that can be used as an efficient screening model system for ΔF508-CFTR rescue. RESULTS:CFTR knockout cell lines were generated by Cas9 with a single-guide RNA (sgRNA) targeting exon 1 of the CFTR genome, which produced indels th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2190-4
更新日期:2016-12-01 00:00:00
abstract:OBJECTIVE:Microalgae gained interest for potential use as biodiesel producers, since they synthesize and accumulate significant quantities of lipids. The aim of this work was to isolate indigenous microalgae strains from Greek habitats, study their physicochemical growth conditions and finally select the best ones with...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-019-02658-6
更新日期:2019-05-01 00:00:00
abstract::A new ribonuclease (RNase) with tobacco mosaic virus inhibition was isolated and purified from Bacillus cereus ZH14 through ammonium sulfate precipitation, ultrafiltration, ion-exchange chromatography of DEAE-Sephadex A-50 column, and gel chromatography of Sephacryl S-200HR column. The enzyme was purified approximatel...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9831-1
更新日期:2009-01-01 00:00:00
abstract::A morphological parameter of polygonal index was defined as the ratio of cell adhesion area versus the square of the major cell axis, and cells that had an adhesion area larger than 4000 mum(2) and a polygonal index larger than 0.3 were considered large polygonal cells. Cell morphology tended to change from fibroblast...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9683-8
更新日期:2008-07-01 00:00:00
abstract::A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min(-1) (mg powder)(-1) in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4 degrees C. Solid-state buffers (bases and their HCl salts) suspended in the organic medium d...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000012891.13904.62
更新日期:2004-01-01 00:00:00
abstract:OBJECTIVES:Catalytic efficiency of a nitrogen-doped, mesoporous carbon aerogel cathode catalyst was investigated in a two-chambered microbial fuel cell (MFC) applying graphite felt as base material for cathode and anode, utilizing peptone as carbon source. RESULTS:This mesoporous carbon aerogel containing catalyst lay...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2338-x
更新日期:2017-07-01 00:00:00