Abstract:
:The gene, xynB, from Streptomyces olivaceoviridis A1 encoding xylanase, XYNB, with a high specific activity for xylan, was transformed into potato (Solanum tuberosum L.) by Agrobacterium tumefaciens. The integration of xynB into genomic DNA was confirmed by PCR and reverse transcriptase-PCR. The gene was expressed under the control of a constitutive double cauliflower mosaic virus (CaMV) 35S promoter. Both SDS-PAGE and western blot analysis showed high levels of expression of the 21 kDa and 31 kDa XYNB proteins in transgenic potato plants transformed by the binary vectors pBinXy and signal peptide contained pBinSPXy, respectively. The recombinant XYNB protein was present at up to 5% of total soluble leaf protein in the cytoplasm. In transgenic leaf and tuber extracts, xylanase activity was up to 87 micromol min(-1) g(-1) fresh leaf (9.7 micromol min(-1) mg(-1) total soluble protein). The xylanase was stable at 60 degrees C and 70 degrees C in buffers (pH 5.2) for 5 min. Furthermore, the xylanase enzymatic activity remained virtually unchanged over several generations of potato. These results demonstrate that the transgenic potato can be used to produce recombinant xylanase with high specific enzyme activity and can potentially be an alternative to present-day xylanase additives to animal feed.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Yang P,Wang Y,Bai Y,Meng K,Luo H,Yuan T,Fan Y,Yao Bdoi
10.1007/s10529-006-9280-7subject
Has Abstractpub_date
2007-04-01 00:00:00pages
659-67issue
4eissn
0141-5492issn
1573-6776journal_volume
29pub_type
杂志文章abstract::Although resistance of microorganisms to Hg(II) salts has been widely investigated and resistant strains have been reported from many eubacterial genera, there are few reports of mercuric ion resistance in extremophilic microorganisms. Moderately thermophilic mercury resistant bacteria were selected by growth at 62 de...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2723-8
更新日期:2005-11-01 00:00:00
abstract:OBJECTIVES:To investigated the potential of a novel dendrosomal nanoformulation of curcumin (DNC) in blocking radiation-induced changes in irradiated human umbilical vein endothelial cells (HUVECs), and their adhesion to human THP-1 monocytoid cells. RESULTS:Co60 gamma rays reduced viability, raised the expression of ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2189-x
更新日期:2016-12-01 00:00:00
abstract::Clostridium spp. produce n-butanol in the acetone/butanol/ethanol process. For sustainable industrial scale butanol production, a number of obstacles need to be addressed including choice of feedstock, the low product yield, toxicity to production strain, multiple-end products and downstream processing of alcohol mixt...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-012-0926-3
更新日期:2012-08-01 00:00:00
abstract::The lipase-catalyzed reaction is useful to obtain sugar esters with chemically defined structures and will contribute to the synthesis of sugar-based compounds by a chemo-enzymatic pathway. The synthesis of sugar esters in nonaqueous media has been attempted for a quarter century. To facilitate the reactions, they hav...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-011-0663-z
更新日期:2011-10-01 00:00:00
abstract::The genetic relationship among commercial cultivars of Citrus limon (lemon) was analysed by inter-simple sequence repeats (ISSR) and flow cytometry techniques. Two cultivars with a close germplasm were distinguished by screening 10 SSR primers and by measuring DNA content of prestained nuclei. ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/B:BILE.0000044920.36497.fc
更新日期:2004-08-01 00:00:00
abstract::Many enzymes used as digestive aids exhibit, at best, moderate stability when incubated under gastrointestinal conditions. A supplemental β-galactosidase administered orally to treat lactose intolerance was conjugated to 40 kDa, branched polyethylene glycol (PEG). PEGylation increased the enzyme's relative activity at...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0474-7
更新日期:2011-03-01 00:00:00
abstract::A plasmid, pBGFP, carrying green fluorescent protein (gfp) and benomyl-resistance genes was constructed and transformed into Metarhizium anisopliae. The transformants grew normally and GFP fluorescence was detected. No change was found in virulence for the transformants. Fluorescence was detected in hyphae from the ha...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9332-7
更新日期:2007-06-01 00:00:00
abstract::Flavonoids have been proposed to play diverse roles in plant growth and development, including defense, symbiosis, pollen development and male fertility, polar auxin transport, and protection against ultraviolet radiation. Recently, a new role in cell cycle regulation has emerged. Genetic alteration of glucuronide met...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-005-1521-7
更新日期:2005-03-01 00:00:00
abstract::General methods for identification of usual carotenoids including C18-HPLC retention time and spectroscopic methods, such as absorption spectra, mass spectra, NMR spectra, are briefly summarized. ...
journal_title:Biotechnology letters
pub_type: 信件
doi:10.1007/s10529-014-1479-4
更新日期:2014-06-01 00:00:00
abstract::A recombinant gene coding for an antibody-targeted urokinase-type plasminogen activator was constructed for the purpose of enhancing the thrombolytic specificity of urokinase. The recombinant gene was cloned into prokaryotic expression vector pTrcHisA, and transformed into Escherichia coli strain Rosetta (DE3). Less t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-3185-0
更新日期:2004-11-01 00:00:00
abstract:OBJECTIVE:To evaluate the quantity of Spirulina cultured in seawater, salt-tolerant strains were screened out and their growth and antioxidant accumulation were studied in different salt concentrations RESULTS:Salt tolerance of five Spirulina strains were investigated with modified Zarrouk medium (with 200-800 mM NaCl...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2087-2
更新日期:2016-07-01 00:00:00
abstract::Environmental factors, such as drought, salinity, extreme temperature, ozone poisoning, metal toxicity etc., significantly affect crops. To study these factors and to design a possible remedy, biological experimental data concerning these crops requires the quantification of gene expression and comparative analyses at...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-015-2010-2
更新日期:2016-04-01 00:00:00
abstract::The genus Ajuga L., belonging to Lamiaceae family, is widespread. The demand for Ajuga species has risen sharply because of their medicinal, ornamental, and pharmacological properties. These wide-ranging plants are being rapidly depleted due to over-collection for ornamental and medicinal purposes, as well as by habit...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-017-2376-4
更新日期:2017-09-01 00:00:00
abstract::A purified recombinant enzyme from Spirochaeta thermophila, that is suggested to be a cellobiose 2-epimerase, was a 47 kDa monomer with a specific activity of 29.2 U min(-1) for mannobiose. The epimerization activity of the recombinant enzyme for mannobiose was maximal at pH 7.0 and 60 °C with a half-life of 124 h. Th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1267-6
更新日期:2013-11-01 00:00:00
abstract:OBJECTIVES:To find new metabolic engineering strategies to improve the yield of acetone in Escherichia coli. RESULTS:Results of flux balance analysis from a modified Escherichia coli genome-scale metabolic network suggested that the introduction of a non-oxidative glycolysis (NOG) pathway would improve the theoretical...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2115-2
更新日期:2016-08-01 00:00:00
abstract::Cell-free extracts of Streptomyces sp. DRS I converted ampicillin to cephalexin, presumably due to the activity of the enzyme, expandase. The extract was fractionated and characterized by colorimetric and chromatographic measurements coupled with disc-agar diffusion bioassay against an ampicillin-resistant, cephalexin...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-9973-9
更新日期:2009-07-01 00:00:00
abstract::C3H/HeJ mice were sensitized with ovalbumin (OVA) and choleratoxin (CT) for 5 weeks, and then Bifidobacterium bifidum BGN4 was administered continuously for 7 weeks, starting 2 weeks before (pre-treatment group) and 2 weeks after (post-treatment group) the initial sensitization. After sensitization, the OVA-induced (s...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3682-9
更新日期:2005-09-01 00:00:00
abstract::Human DNA polymerase iota (Pol ι) is a Y-family DNA polymerase with unusual biochemical properties and not fully understood functions. Pol ι preferentially incorporates dGTP opposite template thymine. This property can be used to monitor Pol ι activity in the presence of other DNA polymerases, e.g. in cell extracts of...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0764-8
更新日期:2012-02-01 00:00:00
abstract::At the core of a biomanufacturing process for recombinant proteins is the production cell line. It influences the productivity and product quality. Its characteristics also dictate process development, as the process is optimized to complement the producing cell to achieve the target productivity and quality. Advances...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-015-1843-z
更新日期:2015-08-01 00:00:00
abstract::Recombinant Mycobacterium sp. strain MR65 carrying dszABCD genes was used for desulfurization of 10-methylbenzo[b]naphtho[2,1-d]thiophene (10-methyl BNT) in the hexadecane phase. The specific activity was 25% of that of dibenzothiophene (DBT). One of two major metabolites of 10-methyl BNT produced by strain MR65 was i...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1023584805911
更新日期:2003-05-01 00:00:00
abstract::Quercetin was methylated with two O-methyltransferases (OMTs) expressed in E. coli. A construct (RSOMT) was designed to express two OMTs: ROMT-9, which methylates specifically at the 3'-hydroxyl group of quercetin and SOMT-2, which methylates at the 4'-hydroxyl group. Both OMT genes were driven by T7 promoters and had...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3893-0
更新日期:2005-12-01 00:00:00
abstract:PURPOSE OF WORK:To apply a fluorescent dye as an alternative technique to evaluate the survival of potentially probiotic lactobacilli to bile acids (BA) as first step in the design of probiotic functional foods. The use of lactic acid bacteria (LAB) in the functional food design depends on their ability to survive in t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0564-1
更新日期:2011-07-01 00:00:00
abstract:OBJECTIVES:The purpose of this study was to develop a facile and efficient method to enhance the stability and activity of lactoperoxidase (LPO) by using its immobilization on graphene oxide nanosheets (GO-NS). METHODS:Following the LPO purification from bovine whey, it was immobilized onto functionalized GO-NS using ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2583-7
更新日期:2018-10-01 00:00:00
abstract::Therapies for high-grade gliomas (HHG) that have strong tendency of infiltration and resistance to chemotherapies are currently unavailable. Here, we report that lower-dose combination therapy of Buthus martensii Karsch (BmK) CT, a type of scorpion toxin peptide, and LiCl, clinically used as mo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0741-2
更新日期:2012-01-01 00:00:00
abstract::A number of biosensors have been developed for phosphate analysis particularly, concerning its negative impact within the environmental and biological systems. Enzymatic biosensors comprising either a single or multiple enzymatic system have been extensively used for the direct and indirect analysis of phosphate ions....
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-015-1823-3
更新日期:2015-07-01 00:00:00
abstract::Expression of the desulfurization genes (dsz) in Mycobacterium sp. G3 is repressed by sulfate, which is the product of biodesulfurization. An expression clone, pSMTABC, was constructed by placing the dsz genes downstream of the hsp60 promoter and the constructed plasmid was electroporated into G3. The recombinant stra...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-6721-7
更新日期:2005-06-01 00:00:00
abstract::A dicistronic expression vector was constructed for Chinese hamster ovary (CHO) cells that produce both selectable marker-DHFR (dihydrofolate reductase) gene and recombinant antibody cDNA from a single primary transcript via differential splicing. The vector was derived from a pDHL vector and contained the human const...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2736-3
更新日期:2005-11-01 00:00:00
abstract::A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amy...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5794-7
更新日期:2005-06-01 00:00:00
abstract::Error-prone PCR was used to create more thermoactive and/or thermostable variants of thermoalkalophilic lipases. A variant of the α6 helix (lid domain), with an 189E to V substitution at residue 189, lost its thermostability but exhibited higher activity than that of its wild-type predecessor (r03Lip). Site-saturation...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0635-3
更新日期:2011-09-01 00:00:00
abstract:OBJECTIVE:To evaluate the therapeutic potential of human umbilical cord blood mesenchymal stem cells (hUCBMSCs) on promoting erectile function in a rat model of bilateral cavernous nerve (CN) crush injury. RESULTS:Fifty male Sprague-Dawley rats were randomly assigned to sham + PBS group (n = 10), BCNI (bilateral caver...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1816-2
更新日期:2015-07-01 00:00:00