Abstract:
:Expression of the desulfurization genes (dsz) in Mycobacterium sp. G3 is repressed by sulfate, which is the product of biodesulfurization. An expression clone, pSMTABC, was constructed by placing the dsz genes downstream of the hsp60 promoter and the constructed plasmid was electroporated into G3. The recombinant strain G3-1 desulfurized dibenzothiophene in the presence of 0.5 mM: sulfate while the Dsz phenotype was completely repressed in the wild-type strain. However, there was no significant increase in the amount of desulfurization enzymes in G3-1. In addition, G3 had superior separation of diesel oil-water separation activity compared to E. coli, which is superior to desulfurizing rhodococci.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Takada M,Nomura N,Okada H,Nakajima-Kambe T,Nakahara T,Uchiyama Hdoi
10.1007/s10529-005-6721-7keywords:
subject
Has Abstractpub_date
2005-06-01 00:00:00pages
871-4issue
12eissn
0141-5492issn
1573-6776journal_volume
27pub_type
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/B:BILE.0000044920.36497.fc
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journal_title:Biotechnology letters
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journal_title:Biotechnology letters
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pub_type: 杂志文章
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journal_title:Biotechnology letters
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更新日期:2003-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2004-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2003-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2004-04-01 00:00:00
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pub_type: 杂志文章
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journal_title:Biotechnology letters
pub_type: 杂志文章
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
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更新日期:2019-11-01 00:00:00