Abstract:
OBJECTIVES:To screen the phylogenetically-nearest members of Cellulosimicrobium cellulans for the production of cellulosome-like multienzyme complexes and extracellular β-xylosidase activity against 7-xylosyltaxanes and to get corresponding molecular insights. RESULTS:Cellulosimicrobium (family Promicromonosporaceae) and all genera of the family Cellulomonadeceaec produced both cellulosome-like multienzyme complexes and extracellular β-xylosidase activity, while the other genera of the family Promicromonosporaceae did not. Multiple sequence alignments further indicated that hypothetic protein M768_06655 might be a possible key subunit. CONCLUSION:This is the first report that many actinobacteria species can produce cellulosome-like multienzyme complexes. The production of cellulosome-like complexes and the extracellular β-xylosidase activity against 7-xylosyltaxanes might be used to differentiate the genus Cellulosimicrobium from other genera of the family Promicromonosporaceae.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Wang W,Yu Y,Dou TY,Wang JY,Sun Cdoi
10.1007/s10529-017-2469-0subject
Has Abstractpub_date
2018-02-01 00:00:00pages
335-341issue
2eissn
0141-5492issn
1573-6776pii
10.1007/s10529-017-2469-0journal_volume
40pub_type
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