High-level expression of non-glycosylated and active staphylokinase from Pichia pastoris.

abstract：OBJECTIVES:Exogenous phytase improved the activity of hydrolases to decrease the malting time. RESULTS:Treatment with phytase during barley steeping increased activity of hydrolases (α-/β-amylase, proteinase, β-glucanase and xylanase) in green malt. Maximal activity was observed for α-/β-amylase, β-glucanase and xylan...

journal_title：Biotechnology letters

authors： Qiu R,Lu J

更新日期：2017-12-01 00:00:00

abstract：:Microbial fuel cells can be designed to remove nitrogenous compounds out of wastewater, but their performance is at present limited to 0.33 kg NO(3) (-)-Nm(-3) net cathode compartment (NCC) d(-1). By maintaining the pH in the cathode at 7.2, nitrogen removal was increased from 0.22 to 0.50 kg NO(3) (-)-Nm(-3) NCC d(-1...

journal_title：Biotechnology letters

authors： Clauwaert P,Desloover J,Shea C,Nerenberg R,Boon N,Verstraete W

更新日期：2009-10-01 00:00:00

abstract：:Three biological activities of 12 soy isoflavones were investigated in terms of the structure-activity relationship. Seven molecular descriptors and one absorption predictor were significantly related to the inhibition of cytochrome P4501 activity in HepG2 cells by soy isoflavones, yielding water solubility [correlati...

journal_title：Biotechnology letters

authors： Chun HS,Chang HJ,Choi EH,Kim HJ,Ku KH

更新日期：2005-08-01 00:00:00

abstract：:L-Lactic acid was produced from raw cassava starch, by simultaneous enzyme production, starch saccharification and fermentation in a circulating loop bioreactor with Aspergillus awamori and Lactococcus lactis spp. lactis immobilized in loofa sponge. A. awamori was immobilized directly in cylindrical loofa sponge while...

journal_title：Biotechnology letters

authors： Roble ND,Ogbonna JC,Tanaka H

更新日期：2003-07-01 00:00:00

abstract：:L-threo-3,4-Dihydroxyphenylserine (DOPS) is a chiral unnatural beta-hydroxy amino acid used for the treatment of Parkinson disease. We developed a continuous bioconversion system for DOPS production that uses whole-cell biocatalyst of recombinant Escherichia coli expressing L-threonine aldolase (L-TA) genes cloned fro...

journal_title：Biotechnology letters

authors： Baik SH,Yoshioka H

更新日期：2009-03-01 00:00:00

abstract：:A new full-length beta-1,3-glucanase cDNA, MpGlu, was isolated from a plantain (Musa paradisica) by the rapid amplification of cDNA ends (RACE) technique. Recombinant GST-MpGlu protein, expressed in E. coli, hydrolyzed (1-->3),(1-->6)-beta-glucan of Laminaria digitata and inhibited the growth of Fusarium oxysporum fsp...

journal_title：Biotechnology letters

authors： Jin X,Feng D,Wang H,Wang J

更新日期：2007-09-01 00:00:00

abstract：:Gluconobacter oxydans LMG 1489 was selected as the best strain for NAD(P)-dependent polyol dehydrogenase production. The highest enzyme activities were obtained when this strain was cultivated on a medium consisting of 30 g glycerol l(-1), 7.2 g peptone l(-1) and 1.8 g yeast extract l(-1). Two D-fructose reducing, NAD...

journal_title：Biotechnology letters

authors： Parmentier S,Beauprez J,Arnaut F,Soetaert W,Vandamme EJ

更新日期：2005-03-01 00:00:00

abstract：OBJECTIVES:To investigate antioxidative and biotransformation enzyme responses in Mucor hiemalis towards cyanotoxins considering its use in mycoremediation applications. RESULTS:Catalase (CAT), glutathione reductase (GR), and glutathione peroxidase (GPx) in M. hiemalis maintained their activities at all tested microcy...

journal_title：Biotechnology letters

authors： Balsano E,Esterhuizen-Londt M,Hoque E,Lima SP

更新日期：2017-08-01 00:00:00

abstract：:We prepared ATP photosynthetic vesicles from inside-out membranes of Escherichia coli cells that express delta-rhodopsin (a novel light-driven H(+) transporter) and TF(0)F(1)-ATP synthase (a thermo-stable ATP synthase). These vesicles showed light-dependent ATP synthesis. Furthermore, coupling the ATP photosynthetic v...

journal_title：Biotechnology letters

authors： Hara KY,Suzuki R,Suzuki T,Yoshida M,Kino K

更新日期：2011-06-01 00:00:00

abstract：:Treatment of a hyperthermophilic enzyme, alkaline phosphatase from Pyrococcus furiosus (PfuAP), with EDTA completely deactivated PfuAP, indicating that the presence of one or more divalent metal ions is essential for its catalytic activity. Subsequent addition of various divalent metal ions to the apoprotein recovered...

journal_title：Biotechnology letters

authors： Minamihata K,Goto M,Kamiya N

更新日期：2012-11-01 00:00:00

abstract：OBJECTIVES:To investigate the effect of the linker region (LR) on the enzymatic activity, stability, and flexibility of Bacillus subtilis cellulase CelI15, six mutants were constructed that contained increasing numbers of the LR. RESULTS:The CelI15 mutant with three copies of the LR (approx. 57 amino acids) showed the...

journal_title：Biotechnology letters

authors： Li P,Zhou Y,Li Q,Zhang C,Sun Z,Tian L,Weng H

更新日期：2016-09-01 00:00:00

abstract：OBJECTIVES:To improve target protein production by manipulating expression levels of alanine racemase in Bacillus licheniformis. RESULTS:The gene of dal was identified to be responsible for alanine racemase function. Based on the selection marker of dal, a food-grade expression system was constructed in B. licheniform...

journal_title：Biotechnology letters

authors： He P,Zhang Z,Cai D,Chen Y,Wang H,Wei X,Li S,Chen S

更新日期：2017-09-01 00:00:00

abstract：:Human granulocyte-macrophage colony stimulating factor (hGM-CSF) is used clinically to treat leucopenia typically caused by cancer chemotherapy or radiotherapy. This study used multiple strategies to obtain very high expression levels of OsrhGM-CSF (14 microg/seed) in rice endosperm. Electron micrographs of immunogold...

journal_title：Biotechnology letters

authors： Ning T,Xie T,Qiu Q,Yang W,Zhou S,Zhou L,Zheng C,Zhu Y,Yang D

更新日期：2008-09-01 00:00:00

abstract：:A sensitive and convenient "visible SYPRO" staining protocol was developed for visualizing proteins after SDS-PAGE. Gels were sensitized with SYPRO Ruby and then stained with the Coomassie Brilliant Blue G-250 protocol (Blue Silver). This combined protocol had similar or better linearity than staining with only SYPRO ...

journal_title：Biotechnology letters

authors： Yang Y,Wang J,Bu D,Zhang L,Li S,Zhou L,Wei H

更新日期：2011-01-01 00:00:00

abstract：:The optimal process for the polysialylation reaction was as follows: polysialicacid (PSA) was activated by periodate oxidation, then coupled to CuZn superoxide dismutase (SOD) with a PSA:SOD molar ratio of 40:1 for 24 h. The resulting polysialylated protein contained 3.9 ± 0.3 mol PSA per mol SOD. SDS-PAGE and atomic ...

journal_title：Biotechnology letters

authors： Wu JR,Lin Y,Zheng ZY,Lin CC,Zhan XB,Shen YQ

更新日期：2010-12-01 00:00:00

abstract：:Washed cells (62 mg) of Kitasatospora sp. GF12 in 4 ml buffer (pH 7) catalyzed the regioselective hydroxylation of 60 mM 1,3-adamantanediol [1,3-ad(OH)(2)] to 30.9 mM 1,3,5-adamantanetriol [1,3,5-ad(OH)(3)] over 120 h at 24 °C. Glycerol at 400 mM was added to the reaction mixture to recycle the intracellular NADH/NADP...

journal_title：Biotechnology letters

authors： Mitsukura K,Yamanaka N,Yoshida T,Nagasawa T

更新日期：2012-09-01 00:00:00

abstract：:Electro-acupuncture (EA) at Fenglong acupoint (ST40) can lower the levels of serum cholesterol and triacylglycerols. To study the hepatic genes responsible for the cholesterol-lowering effect of EA, suppression subtractive hybridization combined with the switch mechanism at the 5'-end of RNA template cDNA synthesis an...

journal_title：Biotechnology letters

authors： Li L,Tan GH,Zhang YZ

更新日期：2012-07-01 00:00:00

abstract：:Growth of Streptococcus zooepidemicus in a 10 l bioreactor with 50 g sucrose/l and 10 g casein hydrolysate/l gave 5-6 g hyaluronic acid/l after 24-28 h. Purification of hyaluronic acid gave a recovery of 65% with the final material having an Mr of approximately 4 x 10(6) Da with less than 0.1% protein. ...

journal_title：Biotechnology letters

authors： Rangaswamy V,Jain D

更新日期：2008-03-01 00:00:00

abstract：:The rpiB gene, encoding ribose-5-phosphate isomerase (RpiB) from Clostridium thermocellum, was cloned and expressed in Escherichia coli. RpiB converted D-psicose into D-allose but it did not convert D-xylose, L-rhamnose, D-altrose or D-galactose. The production of D-allose by RpiB was maximal at pH 7.5 and 65 degrees ...

journal_title：Biotechnology letters

authors： Park CS,Yeom SJ,Kim HJ,Lee SH,Lee JK,Kim SW,Oh DK

更新日期：2007-09-01 00:00:00

abstract：OBJECTIVE:Heterotrophic manganese (Mn)-oxidizing microorganisms responsible for biogenic manganese oxide (Bio-MnOx) production are fastidious. Their enrichment is not easily accomplished by merely adding a soluble organic substrate to non-sterile mixed cultures. The objective of this study was to evaluate polycaprolact...

journal_title：Biotechnology letters

authors： Aoki M,Miyashita Y,Tran PT,Okuno Y,Watari T,Yamaguchi T

更新日期：2021-01-26 00:00:00

abstract：:Leymus chinensis is an important grassland perennial grass. However, its drought tolerance requires to be improved. LEA (late embryogenesis abundant) genes are believed to confer resistance to drought and water deficiency. Using Agrobacterium-mediated transformation, a wheat LEA gene, TaLEA(3), was integrated into L. ...

journal_title：Biotechnology letters

authors： Wang L,Li X,Chen S,Liu G

更新日期：2009-02-01 00:00:00

abstract：:Trichodesmium sp., isolated from the Great Barrier Reef lagoon, was cultured in artificial seawater media containing a range of Fe concentration. Fe additions stimulated growth, N2 fixation, cellular chlorophyll a content, light-saturated chlorophyll a-specific gross photosynthetic capacity (Pmchl a) and the dark resp...

journal_title：Biotechnology letters

authors： Fu FX,Bell PR

更新日期：2003-04-01 00:00:00

abstract：OBJECTIVES:To improve the potential value of feather, which is a valuable protein resource, we have separated and identified antioxidant peptide(s) from feather hydrolysate. RESULTS:Feather hydrolysate was prepared by fermentation with Bacillus subtilis S1-4. Antioxidative peptides were separated by sequential acid pr...

journal_title：Biotechnology letters

authors： Wan MY,Dong G,Yang BQ,Feng H

更新日期：2016-04-01 00:00:00

abstract：:The biomacromolecule, gelatin, has increasingly been used in biomedicine-beyond its traditional use in food and cosmetics. The appealing advantages of gelatin, such as its cell-adhesive structure, low cost, off-the-shelf availability, high biocompatibility, biodegradability and low immunogenicity, among others, have m...

journal_title：Biotechnology letters

authors： Su K,Wang C

更新日期：2015-11-01 00:00:00

abstract：:Promoters belong to the most important regulatory domains in genomic sequences. A novel cloning method is described to produce various DNA fragments with the average size about 100 bp, which differ by 1 bp, for screening of the osmotic pressure-inducible promoters from the genome of Escherichia coli. An osmotic pressu...

journal_title：Biotechnology letters

authors： Wang Z,Jin L,Wegrzyn G,Wegrzyn A

更新日期：2008-04-01 00:00:00

abstract：:The ability of Aspergillus strains to utilize thin stillage to produce malic acid was compared. The highest malic acid was produced by Aspergillus niger ATCC 9142 at 17 g l(-1). Biomass production from thin stillage was similar with all strains but ATCC 10577 was the highest at 19 g l(-1). The highest malic acid yield...

journal_title：Biotechnology letters

authors： West TP

更新日期：2011-12-01 00:00:00

abstract：:Hydrophobin-fused domain III of dengue envelope proteins serotypes 1 and 2 were expressed in Rachiplusia nu larvae and purified by aqueous two-phase system. This biotechnological approach of hydrophobin-fused proteins, which allowed obtaining 97.7 µg/larva of fusion protein DomIII serotype 1 and 61.4 µg/larva of fusio...

journal_title：Biotechnology letters

authors： Cerezo J,Targovnik AM,Smith ME,González Maglio D,Luppo VC,Morales MA,Miranda MV,Rodríguez Talou J

更新日期：2020-03-01 00:00:00

abstract：:The shuttle vector pGYC4α (6,157 bp) was constructed based on the sigma-replicon plasmid pYC2 from Lactobacillus sakei BM5 isolated from kimchi. The vector contained inserts of the ColE1 replicon, α-amylase gene from Bacillus licheniformis containing its own signal peptide, and lactococcal promoter P32. Transformation...

journal_title：Biotechnology letters

authors： Yang EJ,Chang HC

更新日期：2011-03-01 00:00:00

abstract：OBJECTIVES:To develop a rapid, dual-parameter, plate-based screening process to improve production and secretion rate of glucose oxidase simultaneously in Aspergillus niger. RESULTS:A morphology engineering based on CaCO3 was implemented, where the yield of GOD by A. niger was increased by up to 50%. Analysis of extra...

journal_title：Biotechnology letters

authors： Zhu X,Sun J,Chu J

更新日期：2018-01-01 00:00:00

abstract：:Biodesulfurization (BDS) in a bioreactor packed with a catalytic bed of silica containing immobilized Rhodococcus rhodochrous was studied. Various bed lengths and support particle sizes were evaluated for BDS of dibenzothiophene (DBT) and gas oil. The sulfur-containing substrates were introduced separately into the bi...

journal_title：Biotechnology letters

authors： Alejandro Dinamarca M,Orellana L,Aguirre J,Baeza P,Espinoza G,Canales C,Ojeda J

更新日期：2014-08-01 00:00:00