听力与言语-语言病理学

行为科学

医学伦理学

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  • Establishment and optimization of a high-throughput mimic perfusion model in ambr® 15.

    abstract:OBJECTIVES:To establish an automated high-throughput mimic perfusion scale-down model (SDM) in ambr® 15 system. RESULTS:An optimized SDM for mimic perfusion was developed in ambr® 15 system. Cell retention in ambr® 15 was realized by sedimentation and supernatant removal with a retention rate > 95%. Although the SDM c...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-03026-5

    authors: Jin L,Wang ZS,Cao Y,Sun RQ,Zhou H,Cao RY

    更新日期:2021-02-01 00:00:00

  • Improvement of glutathione production by a metabolically engineered Yarrowia lipolytica strain using a small-scale optimization approach.

    abstract:OBJECTIVE:In this study, we aimed to maximize glutathione (GSH) production by a metabolically engineered Yarrowia lipolytica strain using a small-scale optimization approach. RESULTS:A three levels four factorial Box-Behnken Design was used to assess the effect of pH, inulin extract, yeast extract and ammonium sulfate...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-03039-0

    authors: Do DTH,Fickers P,Ben Tahar I

    更新日期:2021-02-01 00:00:00

  • Promoter engineering for high ectoine production in a lower saline medium by Halomonas hydrothermalis Y2.

    abstract:OBJECTIVES:For the stress from fermenters, downstream processing equipment, and wastewater treatment to be alleviated, lowering salt-dependence in the ectoine synthesis process is of great significance in the moderately halotolerant Halomonas hydrothermalis Y2. RESULTS:In H. hydrothermalis Y2, the σ70- and σ38-control...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-021-03084-3

    authors: Li S,Shang Y,Zhao Q,Liu Y,Dong X,Wang W,Yang C

    更新日期:2021-01-27 00:00:00

  • Enrichment of marine manganese-oxidizing microorganisms using polycaprolactone as a solid organic substrate.

    abstract:OBJECTIVE:Heterotrophic manganese (Mn)-oxidizing microorganisms responsible for biogenic manganese oxide (Bio-MnOx) production are fastidious. Their enrichment is not easily accomplished by merely adding a soluble organic substrate to non-sterile mixed cultures. The objective of this study was to evaluate polycaprolact...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-021-03088-z

    authors: Aoki M,Miyashita Y,Tran PT,Okuno Y,Watari T,Yamaguchi T

    更新日期:2021-01-26 00:00:00

  • A brief period of darkness induces changes in fatty acid biosynthesis towards accumulation of saturated fatty acids in Chlorella vulgaris UMT-M1 at stationary growth phase.

    abstract:OBJECTIVE:The effects of a brief (3 days) and prolonged (6 days) period of incubation in darkness and light on the biomass content, lipid content and fatty acid profile in Chlorella vulgaris UMT-M1 were determined. RESULTS:Three days of incubation in darkness increased saturated fatty acid (SFA) content from 34.0 to 4...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-021-03077-2

    authors: Cha TS,Yee W,Phua PSP,Loh SH,Aziz A

    更新日期:2021-01-12 00:00:00

  • Growth of Leucoagaricus gongylophorus Möller (Singer) and production of key enzymes in submerged and solid-state cultures with lignocellulosic substrates.

    abstract::The aim of this study was to characterize the growth of the fungus Leucoagaricus gongylophorus LEU18496, isolated from the fungus garden of the nest of leaf cutter ants Atta mexicana. The fungus garden was cultivated in an artificial laboratory nest and the fungus further grown in submerged (SmC) and solid state (SSC)...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-03057-y

    authors: Maya-Yescas ME,Revah S,Le Borgne S,Valenzuela J,Palacios-González E,Terrés-Rojas E,Vigueras-Ramírez G

    更新日期:2021-01-02 00:00:00

  • In vivo delivery of a multiepitope peptide and Nef protein using novel cell-penetrating peptides for development of HIV-1 vaccine candidate.

    abstract:OBJECTIVES:A potent HIV vaccine should overcome some limitations such as polymorphism of human HLA, the diversity of HIV-1 virus, and the lack of an effective delivery system. In this study, a DNA construct encoding Nef60-84, Nef126-144, Vpr34-47, Vpr60-75, Gp16030-53, Gp160308-323, and P248-151 epitopes was designed u...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-03060-3

    authors: Davoodi S,Bolhassani A,Namazi F

    更新日期:2021-01-01 00:00:00

  • Enrichment and detection of circulating tumor cells by immunomagnetic beads and flow cytometry.

    abstract:OBJECTIVE:The purpose of the article is to establish a quick enrichment and detection method using immunomagnetic beads and flow cytometry to analyze circulating tumor cells (CTCs) in the peripheral blood. RESULTS:After incubation with CD326-PE and CD45-APC antibodies, more than 60% MCF7 cells in M-Buffer could be det...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-03007-8

    authors: Hu L,Chen X,Chen M,Fang J,Nie J,Dai H

    更新日期:2021-01-01 00:00:00

  • Screening and identification of a specific peptide binding to breast cancer cells from a phage-displayed peptide library.

    abstract:OBJECTIVES:Breast cancer is a popular fatal malignant tumor for women with high of rates incidence and mortality. Development of the new approaches for breast cancer targeted diagnosis and chemotherapy is emergently needed by the current clinical practice, the important first step is finding a breast cancer specificall...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-03044-3

    authors: Jin H,Gao X,Xiao L,He H,Cheng S,Zhang C,Hou Y,Song F,Su X,Gao Q,Lu Z,Yang R,Song X,Yang J,Duan W,Hou Y

    更新日期:2021-01-01 00:00:00

  • Systematically gap-filling the genome-scale metabolic model of CHO cells.

    abstract:OBJECTIVE:Chinese hamster ovary (CHO) cells are the leading cell factories for producing recombinant proteins in the biopharmaceutical industry. In this regard, constraint-based metabolic models are useful platforms to perform computational analysis of cell metabolism. These models need to be regularly updated in order...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-03021-w

    authors: Fouladiha H,Marashi SA,Li S,Li Z,Masson HO,Vaziri B,Lewis NE

    更新日期:2021-01-01 00:00:00

  • Generation of hyperfunctional recombinant human factor IX variants expressed in human cell line SK-Hep-1.

    abstract:OBJECTIVE:To develop recombinant factor IX (FIX) variants with augmented clotting activity. RESULTS:We generated three new variants, FIX-YKALW, FIX-ALL and FIX-LLW, expressed in SK-Hep-1 cells and characterized in vitro and in vivo. FIX-YKALW showed the highest antigen expression level among the variants (2.17 µg-mL),...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-03040-7

    authors: Sousa Bomfim A,Corrêa de Freitas MC,Picanço Castro V,Abreu Soares Neto M,Pádua R,Covas DT,Sousa Russo EM

    更新日期:2021-01-01 00:00:00

  • Simultaneous detection of multiple mRNAs and proteins in bovine IVD cells and tissue with single cell resolution.

    abstract:OBJECTIVES:Interactions of cells with their neighbors and influences by the surrounding extracellular matrix (ECM) is reflected in a cells transcriptome and proteome. In tissues comprised of heterogeneous cell populations or cells depending on ECM signalling cues such as those of the intervertebral disc (IVD), this inf...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-02997-9

    authors: Li K,Varden L,Henderson A,Lufkin T,Kraus P

    更新日期:2021-01-01 00:00:00

  • Identification of key pathways and differentially expressed genes in bronchopulmonary dysplasia using bioinformatics analysis.

    abstract:OBJECTIVES:The objective of this study was to discover unknown differentially expressed genes (DEGs) associated with bronchopulmonary dysplasia (BPD), analyze their functions and enriched signaling pathways, and identify hub genes correlating with BPD incidence and evolvement. RESULTS:Of 1289 DEGs identified, 568 were...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-02986-y

    authors: Yan W,Jiang M,Zheng J

    更新日期:2020-12-01 00:00:00

  • Enzymatic hydroxylation of L-pipecolic acid by L-proline cis-4-hydroxylases and isomers separation.

    abstract:OBJECTIVES:Establish a complete and efficient method for the preparation of cis-5-hydroxy-L-pipecolic acids (cis-5HPA), including biotransformation and isomers separation and purification. RESULTS:For non-heme Fe(II)/α-KG-dependent dioxygenases, α-ketoglutarate (α-KG) has great influence on the stability of Fe(II) ion...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-03002-z

    authors: Lu F,Chen J,Ye H,Wu H,Sha F,Huang F,Cao F,Wei P

    更新日期:2020-12-01 00:00:00

  • The indigoidine synthetase BpsA provides a colorimetric ATP assay that can be adapted to quantify the substrate preferences of other NRPS enzymes.

    abstract:OBJECTIVES:To develop a colorimetric assay for ATP based on the blue-pigment synthesising non-ribosomal peptide synthetase (NRPS) BpsA, and to demonstrate its utility in defining the substrate specificity of other NRPS enzymes. RESULTS:BpsA is able to convert two molecules of L-glutamine into the readily-detected blue...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-02972-4

    authors: Brown AS,Calcott MJ,Collins VM,Owen JG,Ackerley DF

    更新日期:2020-12-01 00:00:00

  • Augmenting the expression of accD and rbcL genes using optimized iron concentration to achieve higher biomass and biodiesel in Chlorella vulgaris.

    abstract::Chlorella vulgaris is a form of microalgae commonly employed as a biological source of oil for biodiesel production. Major algal cultivation strategies are focused on stimulating growth rate and lipid content. In the present study, the algal growth media was supplemented with iron (III) chloride (FeCl3), as a stimulat...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-02973-3

    authors: Khamoushi A,Tafakori V,Zahed MA,Gayglou SE,Angaji SA

    更新日期:2020-12-01 00:00:00

  • Alginate oligosaccharide protects endothelial cells against oxidative stress injury via integrin-α/FAK/PI3K signaling.

    abstract::Alginate oligosaccharide (AOS) was reported to possess antioxidant and free radical scavenging activities, but the specific effects and mechanisms remain unclear. We investigated the effects of AOS on H2O2-induced oxidative stress and apoptosis in human umbilical vein endothelial cells (HUVECs) and the associated mech...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-03010-z

    authors: Zhao J,Han Y,Wang Z,Zhang R,Wang G,Mao Y

    更新日期:2020-12-01 00:00:00

  • Inhibition of berberine hydrochloride on Candida albicans biofilm formation.

    abstract:OBJECTIVE:This study aimed to investigate the inhibitory effect of berberine hydrochloride (BH) on Candida albicans (C.albicans) ATCC10231 biofilm formation. RESULTS:This paper found a positive correlation between the concentration of BH and its inhibitory effect on the cellular activity of early biofilms because we f...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-02938-6

    authors: Huang X,Zheng M,Yi Y,Patel A,Song Z,Li Y

    更新日期:2020-11-01 00:00:00

  • B1 protein: a novel cell penetrating protein for in vitro and in vivo delivery of HIV-1 multi-epitope DNA constructs.

    abstract:OBJECTIVES:Enhancement of the potential ability of biomacromolecules to cross cell membranes is a critical step for development of effective therapeutic vaccine especially DNA vaccine against human immunodeficiency virus-1 (HIV-1) infection. The supercharged proteins were known as powerful weapons for delivery of diffe...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-02918-w

    authors: Kardani K,Bolhassani A,Agi E,Hashemi A

    更新日期:2020-10-01 00:00:00

  • Characterization of the hydrolysate and catalytic cavity of α-agarase AgaD.

    abstract:OBJECTIVE:To characterize the hydrolysis product and the substrate binding in the catalytic cavity of α-agarase AgaD. RESULTS:The time course curve showed that AgaD degraded agarose by the endo-type cleavage. AgaD did not degrade agarobiose (A2) and agarotetraose (A4). The minimum-length substrate was agarohexaose (A6...

    journal_title:Biotechnology letters

    pub_type: 杂志文章,评审

    doi:10.1007/s10529-020-02901-5

    authors: Wang H,Zhang W,Cui Z,Lu Z,Lu X

    更新日期:2020-10-01 00:00:00

  • Inhibition of Salmonella enteritidis biofilms by Salmonella invasion protein-targeting aptamer.

    abstract::The current study aimed to assess the inhibitory effect of a DNA aptamer (Apt17) which targeted Salmonella invasion proteinA (SipA). The effect of Apt17, on biofilm formation by two Salmonella enteritidis strains, was tested either separately or in combination with ampicillin at different Sub MIC concentrations. Maxim...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-02920-2

    authors: Shatila F,Yaşa İ,Yalçın HT

    更新日期:2020-10-01 00:00:00

  • Recombinant expression of sericin-cecropin fusion protein and its functional activity.

    abstract:OBJECTIVE:Silk sericin is a natural polymer with potential utility in biomedical and biotechnological applications. Recombinantly expressed sericin ensures a source of pure protein with no contamination and with multiple properties when expressed as a fusion protein. Hence, the present paper aims to recombinantly expre...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-02911-3

    authors: Thomas DS,Manoharan C,Rasalkar S,Mishra RK,Gopalapillai R

    更新日期:2020-09-01 00:00:00

  • Reporter gene knock-in into Marc-145 cells using CRISPR/Cas9-mediated homologous recombination.

    abstract:OBJECTIVES:Marc-145 cells (monkey embryonic kidney epithelial cells) play a critical role in the biotechnology industry as certain virus host cells. To investigate the expression of enhanced green fluorescent protein (eGFP) gene as a foreign gene in Marc-145 cells, which we developed an approach of foreign gene site-sp...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-02860-x

    authors: Chang Y,Shao J,Gao Y,Liu W,Gao Z,Hu Y,Chang H

    更新日期:2020-08-01 00:00:00

  • A classical swine fever virus E2 fusion protein produced in plants elicits a neutralizing humoral immune response in mice and pigs.

    abstract::Classical swine fever (CSF) is one of the most important viral diseases of swine worldwide. Although live or attenuated virus vaccines have been used to control CSFV, it is difficult to distinguish vaccinated pigs from infected pigs; this leads to restrictions on import and export. Subunit vaccines based on the CSFV E...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-02892-3

    authors: Park Y,Lee S,Kang H,Park M,Min K,Kim NH,Gu S,Kim JK,An DJ,Choe S,Sohn EJ

    更新日期:2020-07-01 00:00:00

  • Integrated bioinformatics analyses identified SCL3-induced regulatory network in Arabidopsis thaliana roots.

    abstract:OBJECTIVES:To uncover key genes and pathways regulated by SCL3, a GRAS transcription factor, in the context of gibberellin (GA) in the roots of the model plant Arabidopsis thaliana. RESULTS:Gene expression profiles of ga1-3 mutant and ga1-3 and scl3 double mutant are considerably similar to each other, revealed by Pri...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-02850-z

    authors: Weng CY,Zhu MH,Liu ZQ,Zheng YG

    更新日期:2020-06-01 00:00:00

  • A novel Xist RNA-mediated chromosome inactivation model using a mouse artificial chromosome.

    abstract:OBJECTIVE:To develop a mouse artificial chromosome (MAC) carrying the mouse Xist gene (X-inactive specific transcript; Xist-MAC) as a systematic in vitro approach for investigating Xist RNA-mediated chromosome inactivation. RESULTS:Ectopic expression of the Xist gene in CHO cells led to the accumulation of Xist RNA in...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-020-02826-z

    authors: Inaoka D,Sunamura N,Ohira T,Nakayama Y,Kugoh H

    更新日期:2020-05-01 00:00:00

  • The era of 'omics' technologies in the study of microbiologically influenced corrosion.

    abstract::Efforts to elucidate the relationships between microorganisms and metal corrosion were mainly directed to understanding the formation of biofilm structures grown on corroded surfaces. The emergence of high throughput DNA sequencing techniques has helped in the description of microbial species involved directly and ind...

    journal_title:Biotechnology letters

    pub_type: 杂志文章,评审

    doi:10.1007/s10529-019-02789-w

    authors: Procópio L

    更新日期:2020-03-01 00:00:00

  • Simple production of hydrophobin-fused domain III of dengue envelope protein and induction of neutralizing antibodies against the homotypic serotype of dengue virus.

    abstract::Hydrophobin-fused domain III of dengue envelope proteins serotypes 1 and 2 were expressed in Rachiplusia nu larvae and purified by aqueous two-phase system. This biotechnological approach of hydrophobin-fused proteins, which allowed obtaining 97.7 µg/larva of fusion protein DomIII serotype 1 and 61.4 µg/larva of fusio...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-019-02767-2

    authors: Cerezo J,Targovnik AM,Smith ME,González Maglio D,Luppo VC,Morales MA,Miranda MV,Rodríguez Talou J

    更新日期:2020-03-01 00:00:00

  • Violacein@Biogenic Ag system: synergistic antibacterial activity against Staphylococcus aureus.

    abstract:OBJECTIVES:To examine the synergistic antibacterial activity of violacein and silver nanoparticles (AgNPs) against ATCC bacteria, Staphylococcus aureus, Escherichia coli and two bacteria isolated from bovine mastitis. METHODS:Violacein from Chromobacterium violaceum and biogenic AgNPs from Fusarium oxysporum were eval...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-019-02745-8

    authors: Nakazato G,Gonçalves MC,da Silva das Neves M,Kobayashi RKT,Brocchi M,Durán N

    更新日期:2019-12-01 00:00:00

  • Automation in cell and gene therapy manufacturing: from past to future.

    abstract::As more and more cell and gene therapies are being developed and with the increasing number of regulatory approvals being obtained, there is an emerging and pressing need for industrial translation. Process efficiency, associated cost drivers and regulatory requirements are issues that need to be addressed before indu...

    journal_title:Biotechnology letters

    pub_type: 杂志文章,评审

    doi:10.1007/s10529-019-02732-z

    authors: Moutsatsou P,Ochs J,Schmitt RH,Hewitt CJ,Hanga MP

    更新日期:2019-11-01 00:00:00

  • Design and in vitro delivery of HIV-1 multi-epitope DNA and peptide constructs using novel cell-penetrating peptides.

    abstract:OBJECTIVES:Developing an effective HIV vaccine that stimulates the humoral and cellular immune responses is still challenging because of the diversity of HIV-1 virus, polymorphism of human HLA and lack of a suitable delivery system. RESULTS:Using bioinformatics tools, we designed a DNA construct encoding multiple epit...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-019-02734-x

    authors: Davoodi S,Bolhassani A,Sadat SM,Irani S

    更新日期:2019-11-01 00:00:00

  • Degradation of the phenolic β-ether lignin model dimer and dyes by dye-decolorizing peroxidase from Bacillus amyloliquefaciens.

    abstract:OBJECTIVE:The dye-decolorizing peroxidase from Bacillus amyloliquefaciens, BaDyP, was identified to be an efficient catalyst for the degradation of phenolic β-ether lignin model dimer guaiacylglycerol-β-guaiacyl ether (GGE) and dyes. RESULTS:Efeb gene encoding BaDyP from B. amyloliquefaciens MN-13 consisted of 1257 bp...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-019-02696-0

    authors: Yang J,Gao T,Zhang Y,Wang S,Li H,Li S,Wang S

    更新日期:2019-09-01 00:00:00

  • Human genome-derived TOP1 matrix attachment region enhances transgene expression in the transfected CHO cells.

    abstract:OBJECTIVES:To investigate the effect of full-length fragment of DNA topoisomerase I gene (TOP1) matrix attachment regions (MARs) originating from the human genome on transgene expression in Chinese hamster ovary (CHO) cells and explore the underlying mechanisms. RESULTS:Results showed that TOP1 MAR cannot only enhance...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-019-02673-7

    authors: Jia YL,Guo X,Wang XC,Wang TY

    更新日期:2019-07-01 00:00:00

  • Lipid production from indigenous Greek microalgae: a possible biodiesel source.

    abstract:OBJECTIVE:Microalgae gained interest for potential use as biodiesel producers, since they synthesize and accumulate significant quantities of lipids. The aim of this work was to isolate indigenous microalgae strains from Greek habitats, study their physicochemical growth conditions and finally select the best ones with...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-019-02658-6

    authors: Savvides AL,Moisi K,Katsifas EA,Karagouni AD,Hatzinikolaou DG

    更新日期:2019-05-01 00:00:00

  • Plasma nitriding under low temperature improves the endothelial cell biocompatibility of 316L stainless steel.

    abstract:OBJECTIVES:To evaluate the effects of the surface modification of 316L stainless steel (SS) by low-temperature plasma nitriding on endothelial cells for stent applications. RESULTS:X-ray diffraction (XRD) confirmed the incorporation of nitrogen into the treated steel. The surface treatment significantly increased SS r...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-019-02657-7

    authors: Braz JKFS,Martins GM,Sabino V,Vitoriano JO,Barboza CAG,Soares AKMC,Rocha HAO,Oliveira MF,Alves Júnior C,Moura CEB

    更新日期:2019-05-01 00:00:00

  • Green asymmetric reduction of acetophenone derivatives: Saccharomyces cerevisiae and aqueous natural deep eutectic solvent.

    abstract:OBJECTIVE:Chiral building blocks [(S)-1-(3-methylphenyl)ethanol, (S)-1-(3,4-dimethylphenyl)ethanol and (S)-1-(2,4,6-trimethylphenyl)ethanol] for drug synthesis were prepared using two green approaches: (1) the yeast Saccharomyces cerevisiae as the biocatalyst and (2) the natural deep eutectic solvents (NADES) as the al...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-018-2631-3

    authors: Panić M,Delač D,Roje M,Radojčić Redovniković I,Cvjetko Bubalo M

    更新日期:2019-02-01 00:00:00

  • Immobilization of lactoperoxidase on graphene oxide nanosheets with improved activity and stability.

    abstract:OBJECTIVES:The purpose of this study was to develop a facile and efficient method to enhance the stability and activity of lactoperoxidase (LPO) by using its immobilization on graphene oxide nanosheets (GO-NS). METHODS:Following the LPO purification from bovine whey, it was immobilized onto functionalized GO-NS using ...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-018-2583-7

    authors: Shariat SZAS,Borzouee F,Mofid MR,Varshosaz J

    更新日期:2018-10-01 00:00:00

  • The response of transgenic Brassica species to salt stress: a review.

    abstract::Salt stress is considered one of the main abiotic factors to limit crop growth and productivity by affecting morpho-physiological and biochemical processes. Genetically, a number of salt tolerant Brassica varieties have been developed and introduced, but breeding of such varieties is time consuming. Therefore, current...

    journal_title:Biotechnology letters

    pub_type: 杂志文章,评审

    doi:10.1007/s10529-018-2570-z

    authors: Shah N,Anwar S,Xu J,Hou Z,Salah A,Khan S,Gong J,Shang Z,Qian L,Zhang C

    更新日期:2018-08-01 00:00:00

  • Directed evolution and site-specific mutagenesis of L-isoleucine dioxygenase derived from Bacillus weihenstephanensis.

    abstract:OBJECTIVES:L-isoleucine dioxygenase (IDO) specifically transforms L-isoleucine (Ile) to 4-hydroxyisoleucine (4-HIL), and 4-HIL is a promising drug for diabetes. To enhance the activity and catalytic efficiency of IDO, we used directed evolution and site-specific mutagenesis. RESULTS:The IDO gene (ido) derived from Bac...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-018-2566-8

    authors: Huang S,Shi F

    更新日期:2018-08-01 00:00:00

  • A simple method to determine evaporation and compensate for liquid losses in small-scale cell culture systems.

    abstract:OBJECTIVES:Establish a method to indirectly measure evaporation in microwell-based cell culture systems and show that the proposed method allows compensating for liquid losses in fed-batch processes. RESULTS:A correlation between evaporation and the concentration of Na+ was found (R2 = 0.95) when using the 24-well-bas...

    journal_title:Biotechnology letters

    pub_type: 杂志文章

    doi:10.1007/s10529-018-2556-x

    authors: Wiegmann V,Martinez CB,Baganz F

    更新日期:2018-07-01 00:00:00

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