A polysaccharide isolated from the medicinal herb Bletilla striata induces endothelial cells proliferation and vascular endothelial growth factor expression in vitro.

abstract：OBJECTIVES:Establish a complete and efficient method for the preparation of cis-5-hydroxy-L-pipecolic acids (cis-5HPA), including biotransformation and isomers separation and purification. RESULTS:For non-heme Fe(II)/α-KG-dependent dioxygenases, α-ketoglutarate (α-KG) has great influence on the stability of Fe(II) ion...

journal_title：Biotechnology letters

authors： Lu F,Chen J,Ye H,Wu H,Sha F,Huang F,Cao F,Wei P

更新日期：2020-12-01 00:00:00

abstract：:Ethanol, added as a de-emulsifier to separate oil and biocatalyst (or bacterial cells) from a three-phase (oil/biocatalyst/aqueous phase) emulsion, formed in diesel biodesulfurization employing Gordonia nitida, improved oil recovery by centrifugation from about 50% in its absence to almost 100% at 3% (v/v). The biocat...

journal_title：Biotechnology letters

authors： Choi OK,Choi KS,Ryu HW,Chang YK

更新日期：2003-01-01 00:00:00

abstract：:Two new strains of Escherichia coli B were engineered for the production of lactate with no detectable chiral impurity. All chiral impurities were eliminated by deleting the synthase gene (msgA) that converts dihydroxyacetone-phosphate to methylglyoxal, a precursor for both L: (+)- and D: (-)-lactate. Strain TG113 con...

journal_title：Biotechnology letters

authors： Grabar TB,Zhou S,Shanmugam KT,Yomano LP,Ingram LO

更新日期：2006-10-01 00:00:00

abstract：:Promoters belong to the most important regulatory domains in genomic sequences. A novel cloning method is described to produce various DNA fragments with the average size about 100 bp, which differ by 1 bp, for screening of the osmotic pressure-inducible promoters from the genome of Escherichia coli. An osmotic pressu...

journal_title：Biotechnology letters

authors： Wang Z,Jin L,Wegrzyn G,Wegrzyn A

更新日期：2008-04-01 00:00:00

abstract：:The lipase of Pseudomonas cepacia was immobilized onto electrospun polyacrylonitrile (PAN) fibers and used for the conversion of (S)-glycidol with vinyl n-butyrate to glycidyl n-butyrate in isooctane. The rate of reaction with the adsorbed lipase was 23-fold higher than the initial material. After 10 recyclings, the i...

journal_title：Biotechnology letters

authors： Sakai S,Liu Y,Yamaguchi T,Watanabe R,Kawabe M,Kawakami K

更新日期：2010-08-01 00:00:00

abstract：:1-Propyl laurate synthesis should not be used as standard reaction test of immobilized enzymes in microemulsion-based organogels (MBGs) prepared using lecithin/1-propanol as surfactant when extremely active enzymes with high load are used. In these cases, an anomalous kinetic reaction constant value is observed over s...

journal_title：Biotechnology letters

authors： Domínguez de María P,Xenakis A,Stamatis H,Sinisterra JV

更新日期：2004-10-01 00:00:00

abstract：:Arylomycins are type I signal peptidase inhibitors and have a potential as a new type of antibiotics. They were identified from the broth of Streptomyces sp. HCCB10043. The arylomycin biosynthetic gene cluster in this strain was identical to that in S. roseosporus. Within the gene cluster, aryC, encoding a P450 enzyme...

journal_title：Biotechnology letters

authors： Jin X,Rao M,Wei W,Ge M,Liu J,Chen D,Liang Y

更新日期：2012-12-01 00:00:00

abstract：:A novel gene encoding the α-chain of goose immunoglobulin heavy-chain (Igα) was cloned by reverse transcription-PCR. The cDNA had 1,760 bp and encompassed a partial V-D-J region of the heavy chain, a constant region (Cα) and 3'-untranslated region of α-chain. The Cα gene contains four constant region domains (CH1-CH4)...

journal_title：Biotechnology letters

authors： Guo Y,Gao M,Luo X,Sheng Q,Ma B,Wang J

更新日期：2014-04-01 00:00:00

abstract：:Error-prone PCR was used to create more thermoactive and/or thermostable variants of thermoalkalophilic lipases. A variant of the α6 helix (lid domain), with an 189E to V substitution at residue 189, lost its thermostability but exhibited higher activity than that of its wild-type predecessor (r03Lip). Site-saturation...

journal_title：Biotechnology letters

authors： Shih TW,Pan TM

更新日期：2011-09-01 00:00:00

abstract：:The Rho1 protein is a GTPase that participates in cell wall biogenesis. We analyzed the transcript levels of laccase genes (lccl, lcc2, lcc3, lcc4, lcc5, and lcc9), and a xylanase gene (xyl3) in Fusarium oxysporum f. sp. lycopersici strain 4287 (wild type) and two mutant strains; rhol::hyg that lacks a functional Rho1...

journal_title：Biotechnology letters

authors： Reyes-Medina MA,Macías-Sánchez KL

更新日期：2015-03-01 00:00:00

abstract：:C3H/HeJ mice were sensitized with ovalbumin (OVA) and choleratoxin (CT) for 5 weeks, and then Bifidobacterium bifidum BGN4 was administered continuously for 7 weeks, starting 2 weeks before (pre-treatment group) and 2 weeks after (post-treatment group) the initial sensitization. After sensitization, the OVA-induced (s...

journal_title：Biotechnology letters

authors： Kim H,Lee SY,Ji GE

更新日期：2005-09-01 00:00:00

abstract：:The effects of glycerol and the oxygen transfer rate on the xylitol production rate by a xylitol dehydrogenase gene (XYL2)-disrupted mutant of Candida tropicalis were investigated. The mutant produced xylitol near the almost yield of 100% from D: -xylose using glycerol as a co-substrate for cell growth and NADPH regen...

journal_title：Biotechnology letters

authors： Ko BS,Rhee CH,Kim JH

更新日期：2006-08-01 00:00:00

abstract：:Gluconobacter oxydans LMG 1489 was selected as the best strain for NAD(P)-dependent polyol dehydrogenase production. The highest enzyme activities were obtained when this strain was cultivated on a medium consisting of 30 g glycerol l(-1), 7.2 g peptone l(-1) and 1.8 g yeast extract l(-1). Two D-fructose reducing, NAD...

journal_title：Biotechnology letters

authors： Parmentier S,Beauprez J,Arnaut F,Soetaert W,Vandamme EJ

更新日期：2005-03-01 00:00:00

abstract：:Cell division of the wild type strain Corynebacterium (formerly Brevibacterium) ammoniagenes ATCC 6872 which requires 1 microM Mn2+ for balanced growth was inhibited by addition of 20 mM hydroxyurea (HU) or 10 mM p-methoxyphenol (MP) to a Mn2+-supplemented fermentation medium at an appropriate time. Scanning electron ...

journal_title：Biotechnology letters

authors： Abbouni B,Elhariry HM,Auling G

更新日期：2003-01-01 00:00:00

abstract：:Environmental factors, such as drought, salinity, extreme temperature, ozone poisoning, metal toxicity etc., significantly affect crops. To study these factors and to design a possible remedy, biological experimental data concerning these crops requires the quantification of gene expression and comparative analyses at...

journal_title：Biotechnology letters

authors： Gul A,Ahad A,Akhtar S,Ahmad Z,Rashid B,Husnain T

更新日期：2016-04-01 00:00:00

abstract：OBJECTIVES:To analyze the mechanisms underlying the impact of recombinant Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV)-mediated BmK IT expression on the function of baculovirus GP64 envelope fusion protein and progeny virus production. RESULTS:Viral propagation assay indicated that overexpression G...

journal_title：Biotechnology letters

authors： Fu Y,Miao Y,Zheng S,Du J,Liang A

更新日期：2016-10-01 00:00:00

abstract：:Baker's yeast suspensions were incubated at different pressures (from 1 bar to 6 bar) and different gases [air, O(2) and a mixture of 8% (v/v) CO(2), 21% O(2) and N(2)]. Raising the air pressure from 1 bar to 6 bar stimulated cell growth but had no effect on leavening ability or viability of the cells. A 50% reduction...

journal_title：Biotechnology letters

authors： Campelo AF,Belo I

更新日期：2004-08-01 00:00:00

abstract：:In the industrial production of bioethanol from lignocellulosic biomass, a strain of Saccharomyces cerevisiae that can ferment xylose in the presence of inhibitors is of utmost importance. The recombinant, industrial-flocculating S. cerevisiae strain NAPX37, which can ferment xylose, was used as the parent to delete t...

journal_title：Biotechnology letters

authors： Li YC,Gou ZX,Liu ZS,Tang YQ,Akamatsu T,Kida K

更新日期：2014-10-01 00:00:00

abstract：OBJECTIVES:To rationally identify targets for enhancing adenosine production, transcription level of genes involved in adenosine synthesis of Bacillus subtilis XGL was detected during the fermentation process, complemented with metabolite pool analysis. RESULTS:PurR-regulated genes (pur operon and purA) and prs were d...

journal_title：Biotechnology letters

authors： Zhang C,Du S,Liu Y,Xie X,Xu Q,Chen N

更新日期：2015-07-01 00:00:00

abstract：OBJECTIVE:To construct a highly expressed and active MEK1R4F (a constitutively active form of mitogen-activated protein kinase kinase 1) by fusion of soluble adenylate kinase (Adk) tag, resulting in Adk-MEK1R4F protein suitable for preparation of phosphorylated ERK. RESULTS:We fused the Adk to the N-terminus of MEK1R4...

journal_title：Biotechnology letters

authors： Yang F,Li H

更新日期：2017-10-01 00:00:00

abstract：:Clostridium spp. produce n-butanol in the acetone/butanol/ethanol process. For sustainable industrial scale butanol production, a number of obstacles need to be addressed including choice of feedstock, the low product yield, toxicity to production strain, multiple-end products and downstream processing of alcohol mixt...

journal_title：Biotechnology letters

authors： Jurgens G,Survase S,Berezina O,Sklavounos E,Linnekoski J,Kurkijärvi A,Väkevä M,van Heiningen A,Granström T

更新日期：2012-08-01 00:00:00

abstract：:3,4-Dihydroxybenzoate decarboxylase in Enterobacter cloacae P241 was induced by adding 3,4-dihydroxybenzoic acid, 3-hydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid or 4-acetamidobenzoic acid to the culture medium. After stabilizing the enzyme activity by adding 5 mM dithiothreitol and 20 mM Na(2)S(2)O(3) to a cell-f...

journal_title：Biotechnology letters

authors： Yoshida T,Inami Y,Matsui T,Nagasawa T

更新日期：2010-05-01 00:00:00

abstract：:Ganoderic acid, from Ganoderma lucidum, at 8 microg/ml inhibited replication of hepatitis B virus (HBV) in HepG2215 cells over 8 days. Production of HBV surface antigen and HBV e antigen were 20 and 44% of controls without ganoderic acid. Male KM mice were significantly protected from liver injury, induced with carbon...

journal_title：Biotechnology letters

authors： Li YQ,Wang SF

更新日期：2006-06-01 00:00:00

abstract：:Therapies for high-grade gliomas (HHG) that have strong tendency of infiltration and resistance to chemotherapies are currently unavailable. Here, we report that lower-dose combination therapy of Buthus martensii Karsch (BmK) CT, a type of scorpion toxin peptide, and LiCl, clinically used as mo...

journal_title：Biotechnology letters

authors： Fu Y,Zheng S,Huang R,An N,Zheng Y,Zhang Z,Liang A

更新日期：2012-01-01 00:00:00

abstract：:Dielectric barrier discharge plasma was used to generate a stable strain of Klebsiella pneumoniae (designated to as Kp-M2) with improved 1,3-propanediol production. The specific activities of glycerol dehydrogenase, glycerol dehydratase and 1,3-propanediol oxidoreductase in the crude cell extract increased from 0.11, ...

journal_title：Biotechnology letters

authors： Dong XY,Xiu ZL,Li S,Hou YM,Zhang DJ,Ren CS

更新日期：2010-09-01 00:00:00

abstract：:L-threo-3,4-Dihydroxyphenylserine (DOPS) is a chiral unnatural beta-hydroxy amino acid used for the treatment of Parkinson disease. We developed a continuous bioconversion system for DOPS production that uses whole-cell biocatalyst of recombinant Escherichia coli expressing L-threonine aldolase (L-TA) genes cloned fro...

journal_title：Biotechnology letters

authors： Baik SH,Yoshioka H

更新日期：2009-03-01 00:00:00

abstract：:A strain of Serratia sp. showed intracellular electron-transparent inclusion bodies when incubated in the presence of citrate and glycerol 2-phosphate without nitrogen source following pre-growth under carbon-limitation in continuous culture. About 1.3 mmol citrate were consumed per 450 mg biomass, giving a calculated...

journal_title：Biotechnology letters

authors： Lugg H,Sammons RL,Marquis PM,Hewitt CJ,Yong P,Paterson-Beedle M,Redwood MD,Stamboulis A,Kashani M,Jenkins M,Macaskie LE

更新日期：2008-03-01 00:00:00

abstract：:For most cultivated mammalian cells, glutamine is an essential medium component. However, glutamine consumption results in the production of ammonia, a cytotoxic byproduct. Here we investigated the effect of glutamine reduction on recombinant protein production and ammonia accumulation in transiently transfected CHO a...

journal_title：Biotechnology letters

authors： Rajendra Y,Kiseljak D,Baldi L,Hacker DL,Wurm FM

更新日期：2012-04-01 00:00:00

abstract：OBJECTIVE:Chiral building blocks [(S)-1-(3-methylphenyl)ethanol, (S)-1-(3,4-dimethylphenyl)ethanol and (S)-1-(2,4,6-trimethylphenyl)ethanol] for drug synthesis were prepared using two green approaches: (1) the yeast Saccharomyces cerevisiae as the biocatalyst and (2) the natural deep eutectic solvents (NADES) as the al...

journal_title：Biotechnology letters

authors： Panić M,Delač D,Roje M,Radojčić Redovniković I,Cvjetko Bubalo M

更新日期：2019-02-01 00:00:00

abstract：:Streptomyces is an attractive host for heterologous protein secretion. To further optimize its expression capacity, better expression vectors will be helpful. Here, based on pSGL1, a high copy number plasmid present in Streptomyces globisporus C-1027, we constructed a series of novel E. coli-Streptomyces shuttle expre...

journal_title：Biotechnology letters

authors： Zhu Y,Wang L,Du Y,Wang S,Yu T,Hong B

更新日期：2011-02-01 00:00:00