Screening of the osmotic pressure-inducible promoter regions from the whole genome of Escherichia coli by using a novel cloning method.

abstract：:Tandem affinity purification (TAP) is a methodology for the isolation of protein complexes from endogenous sources. It involves incorporation of a dual-affinity tag into the protein of interest and introduction of the construct into desired cell lines or organisms. Using the two affinity handles, the protein complex a...

journal_title：Biotechnology letters

authors： Li Y

更新日期：2011-08-01 00:00:00

abstract：:The industrial-scale production of phenylalanine ammonia-lyase (PAL) mainly uses strains of Rhodotorula. However, the PAL gene from Rhodotorula has not been cloned. Here, the full-length gene of PAL from Rhodotorula glutinis was isolated. It was 2,121 bp, encoding a polypeptide with 706 amino acids and a calculated MW...

journal_title：Biotechnology letters

authors： Zhu L,Cui W,Fang Y,Liu Y,Gao X,Zhou Z

更新日期：2013-05-01 00:00:00

abstract：OBJECTIVE:To improve the production of trans-10,cis-12-conjugated linoleic acid (t10,c12-CLA) from linoleic acid in recombinant Yarrowia lipolytica. RESULTS:Cells of the yeast were permeabilized by freeze/thawing. The optimal conditions for t10,c12-CLA production by the permeabilized cells were at 28 °C, pH 7, 200 rpm...

journal_title：Biotechnology letters

authors： Zhang B,Song Y,Chen H,Zhang H,Chen W

更新日期：2016-11-01 00:00:00

abstract：:The old Annurca apple cultivar (Malus domestica), particularly appreciated for its peculiar flavor and crispy flesh, was studied in order to preserve its ancient germplasm. Twelve clones of Annurca were analyzed using random amplified polymorphic DNA (RAPD) and simple-sequence repeat (SSR) markers. Two out of 30 RAPD ...

journal_title：Biotechnology letters

authors： Iannaccone M,Palumbo D,Ventimiglia I,Patocchi A,Spigno P,Capparelli R

更新日期：2007-02-01 00:00:00

abstract：:Acetic acid is by-product from fermentation processes for producing succinic acid using Mannheimia succiniciproducens . To obtain pure succinic acid from the final fermentation broth, acetic acid was selectively removed based on the different extractability of succinic acid and acetic acid with pH using tri-n-octylami...

journal_title：Biotechnology letters

authors： Huh YS,Hong YK,Hong WH,Chang HN

更新日期：2004-10-01 00:00:00

abstract：OBJECTIVE:The dye-decolorizing peroxidase from Bacillus amyloliquefaciens, BaDyP, was identified to be an efficient catalyst for the degradation of phenolic β-ether lignin model dimer guaiacylglycerol-β-guaiacyl ether (GGE) and dyes. RESULTS:Efeb gene encoding BaDyP from B. amyloliquefaciens MN-13 consisted of 1257 bp...

journal_title：Biotechnology letters

authors： Yang J,Gao T,Zhang Y,Wang S,Li H,Li S,Wang S

更新日期：2019-09-01 00:00:00

abstract：:To explore the feasibility of larch (Larix decidua Mill.) embryogenic cell culture as alternative plant expression system, protein stability in fresh and conditioned medium was characterized in this study and compared to tobacco BY2 suspension culture. Fresh and conditioned media were spiked with 1 microg human IgG an...

journal_title：Biotechnology letters

authors： Calinski A,Classen B,Zoglauer K,Boehm R

更新日期：2009-05-01 00:00:00

abstract：OBJECTIVES:Enhancement of the potential ability of biomacromolecules to cross cell membranes is a critical step for development of effective therapeutic vaccine especially DNA vaccine against human immunodeficiency virus-1 (HIV-1) infection. The supercharged proteins were known as powerful weapons for delivery of diffe...

journal_title：Biotechnology letters

authors： Kardani K,Bolhassani A,Agi E,Hashemi A

更新日期：2020-10-01 00:00:00

abstract：:A purified recombinant enzyme from Spirochaeta thermophila, that is suggested to be a cellobiose 2-epimerase, was a 47 kDa monomer with a specific activity of 29.2 U min(-1) for mannobiose. The epimerization activity of the recombinant enzyme for mannobiose was maximal at pH 7.0 and 60 °C with a half-life of 124 h. Th...

journal_title：Biotechnology letters

authors： Park CS,Kim JE,Lee SH,Kim YS,Kang LW,Oh DK

更新日期：2013-11-01 00:00:00

abstract：:To understand how molecular damage under harsh environmental conditions can be controlled, we investigated the properties of ATPase activity of the chaperonin molecular machinery from the hyperthermophilic archaeon Pyrococcus furiosus (PfCPN). PfCPN ATPase activity depended on K(+) and Mg(2+) and its optimal pH was 7....

journal_title：Biotechnology letters

authors： Chen HY,Tan XL,Lu J,Zhang CX,Zhang Y,Yang SL

更新日期：2009-11-01 00:00:00

abstract：:Targeted gene replacement in the thermotolerant yeast Kluyveromyces marxianus KCTC 17555 has been hampered by its propensity to non-homologous end joining (NHEJ). To enhance homologous recombination (HR) by blocking NHEJ, we identified and disrupted the K. marxianus KU80 gene. The ku80 deletion mutant strain (Kmku80∆)...

journal_title：Biotechnology letters

authors： Choo JH,Han C,Kim JY,Kang HA

更新日期：2014-10-01 00:00:00

abstract：OBJECTIVE:To evaluate the role and the molecular mechanism of miR-30d in non-small cell lung cancer (NSCLC). RESULTS:qRT-PCR was used to detect miR-30d expression in NSCLC tissues and cell lines. miR-30d was frequently down-regulated in NSCLC and its expression was associated with clinicopathological features of NSCLC...

journal_title：Biotechnology letters

authors： Wu Y,Zhang J,Hou S,Cheng Z,Yuan M

更新日期：2017-12-01 00:00:00

abstract：OBJECTIVES:To display a recombinant avidin fused to the autotransporter ShdA to bind biotinylated molecules on the surface of Escherichia coli. RESULTS:Two chimeric protein constructs containing avidin fused to the autotransporter ShdA were expressed on the surface of Escherichia coli DH5α. One fusion protein containe...

journal_title：Biotechnology letters

authors： Pardavé-Alejandre HD,Alvarado-Yaah JE,Pompa-Mera EN,Muñoz-Medina JE,Sárquiz-Martínez B,Santacruz-Tinoco CE,Manning-Cela RG,Ortíz-Navarrete V,López-Macías C,González-Bonilla CR

更新日期：2018-03-01 00:00:00

abstract：:1,3-Propanediol production by Klebsiella pneumoniae was studied in batch cultures under N2 flow and four airflow systems. Different byproducts were formed under different aeration conditions. An anaerobic/aerobic combined fed-batch culture was developed giving 70 g 1,3-propanediol l(-1) and 16 g 2,3-butanediol l(-1) w...

journal_title：Biotechnology letters

authors： Cheng KK,Liu DH,Sun Y,Liu WB

更新日期：2004-06-01 00:00:00

abstract：:Streptomyces is an attractive host for heterologous protein secretion. To further optimize its expression capacity, better expression vectors will be helpful. Here, based on pSGL1, a high copy number plasmid present in Streptomyces globisporus C-1027, we constructed a series of novel E. coli-Streptomyces shuttle expre...

journal_title：Biotechnology letters

authors： Zhu Y,Wang L,Du Y,Wang S,Yu T,Hong B

更新日期：2011-02-01 00:00:00

abstract：:Microbial solar biofuels offer great promise for future sustainable food, fuels and chemicals but are limited by low productivities and a requirement for large land areas to harvest sunlight. A 71 % increase in combined photosynthetic activity was achieved by illuminating both Rhodobacter sphaeroides and Arthrospira (...

journal_title：Biotechnology letters

authors： Redwood MD,Dhillon R,Orozco RL,Zhang X,Binks DJ,Dickinson M,Macaskie LE

更新日期：2012-12-01 00:00:00

abstract：:The gene for beta-agarase of an Agarivorans sp. JA-1 was expressed in Bacillus subtilis strain DB104 for efficient and economical mass-production of the enzyme. We isolated 360 mg protein with a specific activity of 201 U/mg from the culture broth. The efficiency of production was approximately 130-fold higher than th...

journal_title：Biotechnology letters

authors： Lee DG,Jang MK,Lee OH,Kim NY,Ju SA,Lee SH

更新日期：2008-05-01 00:00:00

abstract：:Electroporation of Litopenaeus schmitti embryos was used to transfer the pE300tiGH15 plasmid that contains the tilapia growth hormone gene (tiGH) complexed with a nuclear localization signal peptide into the zygotes. The gene construct was detected in 35 (36%) of the 98 larvae screened by PCR and Southern blot analyse...

journal_title：Biotechnology letters

authors： Arenal A,Pimentel R,Pimentel E,Martín L,Santiesteban D,Franco R,Aleström P

更新日期：2008-05-01 00:00:00

abstract：OBJECTIVES:For the stress from fermenters, downstream processing equipment, and wastewater treatment to be alleviated, lowering salt-dependence in the ectoine synthesis process is of great significance in the moderately halotolerant Halomonas hydrothermalis Y2. RESULTS:In H. hydrothermalis Y2, the σ70- and σ38-control...

journal_title：Biotechnology letters

authors： Li S,Shang Y,Zhao Q,Liu Y,Dong X,Wang W,Yang C

更新日期：2021-01-27 00:00:00

abstract：:The astaxanthin biosynthesis gene cluster in Paracoccus haeundaensis consists of six genes: crtW, crtZ, crtY, crtI, crtB, and crtE contain 726, 486, 1158, 1503, 912, and 879 base pairs, respectively. Individual carotenoid biosynthesis genes of P. haeundaensis have now been expressed in E. coli and each gene product ha...

journal_title：Biotechnology letters

authors： Lee JH,Kim YT

更新日期：2006-08-01 00:00:00

abstract：:Recombinant human interleukin-1 receptor antagonist (rHuIL-1ra) was produced in E. coli as an inclusion body. rHuIL-1ra was purified to Over 98% purity by anion exchange chromatography after on-column refolding. The optimized processes produced more than 2 g pure refolded rHuIL-1ra per 1 l culture, corresponding to a ...

journal_title：Biotechnology letters

authors： Tan H,Dan G,Gong H,Cao L

更新日期：2005-08-01 00:00:00

abstract：:Two new strains of Escherichia coli B were engineered for the production of lactate with no detectable chiral impurity. All chiral impurities were eliminated by deleting the synthase gene (msgA) that converts dihydroxyacetone-phosphate to methylglyoxal, a precursor for both L: (+)- and D: (-)-lactate. Strain TG113 con...

journal_title：Biotechnology letters

authors： Grabar TB,Zhou S,Shanmugam KT,Yomano LP,Ingram LO

更新日期：2006-10-01 00:00:00

abstract：:Biodesulfurization (BDS) in a bioreactor packed with a catalytic bed of silica containing immobilized Rhodococcus rhodochrous was studied. Various bed lengths and support particle sizes were evaluated for BDS of dibenzothiophene (DBT) and gas oil. The sulfur-containing substrates were introduced separately into the bi...

journal_title：Biotechnology letters

authors： Alejandro Dinamarca M,Orellana L,Aguirre J,Baeza P,Espinoza G,Canales C,Ojeda J

更新日期：2014-08-01 00:00:00

abstract：OBJECTIVES:To exploit cold-inducible biochemical processes beneficial for foreign mRNA transcription, translation and storage, as well as protein product stability, during Agrobacterium-mediated transient expression. RESULTS:The efficiency of three different 5'-regulatory sequences to achieve transient expression of t...

journal_title：Biotechnology letters

authors： Gerasymenko IM,Sheludko YV

更新日期：2017-07-01 00:00:00

abstract：:Hydrophobin-fused domain III of dengue envelope proteins serotypes 1 and 2 were expressed in Rachiplusia nu larvae and purified by aqueous two-phase system. This biotechnological approach of hydrophobin-fused proteins, which allowed obtaining 97.7 µg/larva of fusion protein DomIII serotype 1 and 61.4 µg/larva of fusio...

journal_title：Biotechnology letters

authors： Cerezo J,Targovnik AM,Smith ME,González Maglio D,Luppo VC,Morales MA,Miranda MV,Rodríguez Talou J

更新日期：2020-03-01 00:00:00

abstract：:Chlorella protothecoides, a lutein-producing microalga, was grown aerobically in a mineral medium prepared with 70% (v/v) deuterated water. HPLC/atmospheric pressure chemical ionization-mass spectrometry (HPLC/APCI-MS) analysis revealed approximately 58% replacement of hydrogen by deuterium atoms as indicated by the m...

journal_title：Biotechnology letters

authors： Bhosale P,Serban B,Bernstein PS

更新日期：2006-09-01 00:00:00

abstract：:Cell division of the wild type strain Corynebacterium (formerly Brevibacterium) ammoniagenes ATCC 6872 which requires 1 microM Mn2+ for balanced growth was inhibited by addition of 20 mM hydroxyurea (HU) or 10 mM p-methoxyphenol (MP) to a Mn2+-supplemented fermentation medium at an appropriate time. Scanning electron ...

journal_title：Biotechnology letters

authors： Abbouni B,Elhariry HM,Auling G

更新日期：2003-01-01 00:00:00

abstract：:Interactions between ectomycorrhizal fungi (Suillus laricinus, S. tomentosus, Amanita vaginata and Gomphidius viscidus) and the pathogen Rhizoctonia solani in co-culture were studied using both light and scanning electron microscopy. S. laricinus, S. tomentosus and A. vaginata inhibited the growth of the pathogen. Mor...

journal_title：Biotechnology letters

authors： Tang M,Zhang RQ,Chen H,Zhang HH,Tian ZQ

更新日期：2008-10-01 00:00:00

abstract：:miR-21 is highly expressed in a variety of cancers, suggesting that it might play a role in the process of oncogenesis, as supported by it directly causing pre-B cell lymphomas in transgenic mice. Rm21LG transgenic mice for the conditional co-expression of miR-21 and luciferase (Luc) mediated by Cre/lox P system were ...

journal_title：Biotechnology letters

authors： Du T,Jia J,Lin X,Xie R,Li J,Xiao D,Xu K

更新日期：2014-01-01 00:00:00

abstract：:Ochrobactrum anthropi cleaved the delta-(D-alpha-aminoadipoyl)-side chain from delta-(D-alpha-aminoadipoyl)-7-amino-4-methylcoumarin, a beta-lactamase-resistant cephalosporin C analogue. In whole cell conversions up to 1 nkat g(-1) dry cell wt were achieved. O. anthropi possesses also gamma-D-glutamyltranspeptidase ac...

journal_title：Biotechnology letters

authors： Patett F,Fischer L

更新日期：2005-12-01 00:00:00