Abstract:
:The astaxanthin biosynthesis gene cluster in Paracoccus haeundaensis consists of six genes: crtW, crtZ, crtY, crtI, crtB, and crtE contain 726, 486, 1158, 1503, 912, and 879 base pairs, respectively. Individual carotenoid biosynthesis genes of P. haeundaensis have now been expressed in E. coli and each gene product has been purified to homogeneity. Their molecular characteristics, including enzymatic activities, are reported here.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Lee JH,Kim YTdoi
10.1007/s10529-006-9072-0subject
Has Abstractpub_date
2006-08-01 00:00:00pages
1167-73issue
15eissn
0141-5492issn
1573-6776journal_volume
28pub_type
杂志文章abstract::Salt stress is considered one of the main abiotic factors to limit crop growth and productivity by affecting morpho-physiological and biochemical processes. Genetically, a number of salt tolerant Brassica varieties have been developed and introduced, but breeding of such varieties is time consuming. Therefore, current...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-018-2570-z
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVES:Catalytic efficiency of a nitrogen-doped, mesoporous carbon aerogel cathode catalyst was investigated in a two-chambered microbial fuel cell (MFC) applying graphite felt as base material for cathode and anode, utilizing peptone as carbon source. RESULTS:This mesoporous carbon aerogel containing catalyst lay...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2338-x
更新日期:2017-07-01 00:00:00
abstract::Angiogenin, a potent angiogenic factor, was cloned and expressed by Escherichia coli and then purified with gel filtration chromatography. Approximately 90% pure angiogenin was obtained to generate a monoclonal antibody. Using western immunoblotting and ELISA, we confirmed that monoclonal antibody C46 secreted from hy...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9651-3
更新日期:2008-06-01 00:00:00
abstract::Several alkyl-substituted methoxysilanes were evaluated as potential activity and stability enhancing agents for biomimetic silicification of Rhodosporidium toruloides D-amino acid oxidase (RtDAO). When methyl-substituted silanes along with tetramethoxysilane were used as silicic acid precursors for polyallylamine (PA...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0924-5
更新日期:2012-08-01 00:00:00
abstract::A gene-specific, metagenomic PCR method has led to the discovery of a novel esterase subfamily consisting of five homologous members. Sequence analysis of this esterase subfamily, named the ArmEst subfamily, revealed a unique conserved pattern with a significant variable interior sequence flanked by two symmetric and ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1293-4
更新日期:2013-11-01 00:00:00
abstract::Anthocyanic vacuolar inclusions (AVIs) appear as dark red-to-purple spheres of various sizes in vacuoles of grapevine (Vitis vinifera L.) cell suspension culture due to their interaction with anthocyanins. AVIs were purified and the bound anthocyanins extracted and analysed by HPLC from two lines of V. vinifera isolat...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024028603089
更新日期:2003-06-01 00:00:00
abstract::δ-Integration can improve the expression stability and increase the copy number of exogenous genes in Saccharomyces cerevisiae. Generally, δ-integration vectors employ auxotrophic markers, such as LEU2-d, HIS3, TRP1, and URA3, to screen transformants. In this study, two cellulase genes (β-glucosidase and endoglucanase...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1205-7
更新日期:2013-08-01 00:00:00
abstract::The gene encoding a novel short-chain alcohol dehydrogenase in the thermophilic bacterium, Carboxydothermus hydrogenoformans, was identified and overexpressed in Escherichia coli. The enzyme was thermally stable and displayed the highest activity at 70 °C and pH 6.0. It preferred NAD(H) over NADP(H) as a cofactor and ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1082-5
更新日期:2013-03-01 00:00:00
abstract::A new aqueous two phase system comprising polyethylene glycol and xanthan is reported together with its phase diagram. The phase composition of the bottom phase did not vary (PEG 1.6-1.8% w/w; xanthan 0.24-0.28% w/w) while that of the top phase varied significantly (PEG 4-5% w/w, xanthan 0.05-1.37% w/w). Unlike conven...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-4682-5
更新日期:2006-01-01 00:00:00
abstract:OBJECTIVE:A bio-based process is appealing for purification of L-lactic acid, the major enantiomer of polylactic acid syrup, generated by thermochemical processes at the end of life of PLA-based plastics, from its chiral impurity, D-lactic acid, before reuse. RESULTS:Polylactic acid (PLA), a renewable alternative to p...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1924-z
更新日期:2015-12-01 00:00:00
abstract::In Klebsiella pneumoniae, aldehyde dehydrogenases (ALDH) convert 3-hydroxypropionaldehyde (3-HPA) into 3-hydroxypropionic acid (3-HP). Although ALDHs can increase the production of 3-HP in K. pneumoniae, the substrate specificity of ALDH homologues from other microorganisms toward 3-HPA is less documented. Here we rep...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1730-z
更新日期:2015-03-01 00:00:00
abstract:OBJECTIVE:The purpose of the article is to establish a quick enrichment and detection method using immunomagnetic beads and flow cytometry to analyze circulating tumor cells (CTCs) in the peripheral blood. RESULTS:After incubation with CD326-PE and CD45-APC antibodies, more than 60% MCF7 cells in M-Buffer could be det...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03007-8
更新日期:2021-01-01 00:00:00
abstract::We have previously described a model to implant dissociated cells into a cylindrical, vascularized bed in vivo to promote the formation of functional cardiac muscle constructs. We now investigate the cellular organization and the ability of the constructs to generate intra-luminal pressure. Primary cardiac cells were ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9859-2
更新日期:2009-02-01 00:00:00
abstract::Over-expression of the gene, mshA, coding for mycothiol glycosyl transferase improved the robustness of Corynebacterium glutamicum to various stresses. Intracellular mycothiol (MSH) content was increased by 114 % in WT(pXMJ19-mshA) compared to WT(pXMJ19). Survival rates increased by 44, 39, 90, 77, 131, 87, 52, 47, 57...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1501-x
更新日期:2014-07-01 00:00:00
abstract::An efficient method for creating a DNA library is presented in which gene mutagenesis and recombination can be introduced by integrating error-prone PCR with a staggered extension process in one test tube. In this process, less than 15 cycles of error-prone PCR are used to introduce random mutations. After precipitate...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9674-9
更新日期:2008-07-01 00:00:00
abstract::Trichodesmium sp., isolated from the Great Barrier Reef lagoon, was cultured in artificial seawater media containing a range of Fe concentration. Fe additions stimulated growth, N2 fixation, cellular chlorophyll a content, light-saturated chlorophyll a-specific gross photosynthetic capacity (Pmchl a) and the dark resp...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1023068232375
更新日期:2003-04-01 00:00:00
abstract::Most animal cell culture media can be buffered using bicarbonate and high pressure CO(2) in a closed system. However, in an open system, the pH of the culture media increases continuously due to the marked difference in CO(2) pressure between the culture media and the atmosphere. Therefore, it is important to measure ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0341-6
更新日期:2010-11-01 00:00:00
abstract::The rpiB gene, encoding ribose-5-phosphate isomerase (RpiB) from Clostridium thermocellum, was cloned and expressed in Escherichia coli. RpiB converted D-psicose into D-allose but it did not convert D-xylose, L-rhamnose, D-altrose or D-galactose. The production of D-allose by RpiB was maximal at pH 7.5 and 65 degrees ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9393-7
更新日期:2007-09-01 00:00:00
abstract::Biosurfactants could potentially outperform traditional surfactants in many applications whilst being more sustainable to source, manufacture, use and dispose of. However, currently available fermentation production methods are too inefficient to manufacture biosurfactants for these high volume markets. Foaming in an ...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-011-0782-6
更新日期:2012-02-01 00:00:00
abstract::A non-characterized gene, previously proposed as the D-tagatose-3-epimerase gene from Rhodobacter sphaeroides, was cloned and expressed in Escherichia coli. Its molecular mass was estimated to be 64 kDa with two identical subunits. The enzyme specificity was highest with D-fructose and decreased for other substrates i...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-9942-3
更新日期:2009-06-01 00:00:00
abstract:OBJECTIVES:The potential of CRISPR/Cas9 gene editing to repress CyHV-3 was tested in vitro. RESULTS:By targeting two basic target genes necessary for the early transcription of CyHV-3, we show that virus transcription and particle release were significantly decreased by CRISPR/Cas9, as measured by quantitative real-ti...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-2020-0
更新日期:2016-04-01 00:00:00
abstract::Tempe was prepared using mixtures of natural soybean and genetically modified Roundup Ready (RUR) soybean fermented with natural Rhizopus sp. The amount of RUR soybean was quantified using an ELISA plate test. The RUR signal decreased during fermentation. In the control experiments on fermentation of non-RUR soybean, ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025449902523
更新日期:2003-09-01 00:00:00
abstract::KGLP-1, a 31-amino acid glucagon-like peptide-1 (GLP-1) analogue, has a great therapeutic potential for anti-diabetes. In this work, a strategy for expression and purification of functional KGLP-1 peptide has been established. KGLP-1 cDNA was fused with glutathione S-transferase (GST), with an enterokinase cleavage si...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1526-1
更新日期:2014-08-01 00:00:00
abstract:OBJECTIVES:To improve target protein production by manipulating expression levels of alanine racemase in Bacillus licheniformis. RESULTS:The gene of dal was identified to be responsible for alanine racemase function. Based on the selection marker of dal, a food-grade expression system was constructed in B. licheniform...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2359-5
更新日期:2017-09-01 00:00:00
abstract::The use of H(2), He and O(2) during batch fermentation of Saccharomyces cerevisiae BRAS291 increased the final intracellular glycogen contents of the cells from 2-fold to 10-fold compared with a gas-free condition, and this depended on the gas applied. Differently, the intracellular trehalose contents increased from 2...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9533-0
更新日期:2008-02-01 00:00:00
abstract::The expression of secreted proteins in filamentous fungi is down-regulated by a transcriptional feedback mechanism under endoplasmic reticulum stress, termed repression under secretion stress (RESS). To investigate the RESS mechanism, we analyzed the expression of the Taka-amylase A gene (amyB) in Aspergillus oryzae, ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1691-2
更新日期:2015-02-01 00:00:00
abstract:OBJECTIVE:To evaluate the quantity of Spirulina cultured in seawater, salt-tolerant strains were screened out and their growth and antioxidant accumulation were studied in different salt concentrations RESULTS:Salt tolerance of five Spirulina strains were investigated with modified Zarrouk medium (with 200-800 mM NaCl...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2087-2
更新日期:2016-07-01 00:00:00
abstract:OBJECTIVES:Marc-145 cells (monkey embryonic kidney epithelial cells) play a critical role in the biotechnology industry as certain virus host cells. To investigate the expression of enhanced green fluorescent protein (eGFP) gene as a foreign gene in Marc-145 cells, which we developed an approach of foreign gene site-sp...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-02860-x
更新日期:2020-08-01 00:00:00
abstract::A Simplified version of rate equations for enzyme-catalyzed reactions has been developed in which the rate equation is analyzed with two different mechanisms: ordered bi-ter and Ping-Pong bi-bi mechanisms. This procedure is able to develop the rate expressions accurately. Random sequential order mechanism can be effec...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9172-x
更新日期:2006-12-01 00:00:00
abstract:OBJECTIVE:To examine the role of a gene encoding flavin-containing monooxygenase (cFMO) from Corynebacterium glutamicum ATCC13032 when cloned and expressed in Escherichia coli for the production of indigo pigments. RESULTS:The blue pigments produced by recombinant E. coli were identified as indigo and indirubin. The c...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1824-2
更新日期:2015-08-01 00:00:00