Abstract:
:The expression of secreted proteins in filamentous fungi is down-regulated by a transcriptional feedback mechanism under endoplasmic reticulum stress, termed repression under secretion stress (RESS). To investigate the RESS mechanism, we analyzed the expression of the Taka-amylase A gene (amyB) in Aspergillus oryzae, which was depressed under secreted protein stress. We conducted a truncation and deletion analysis of the amyB promoter to identify cis-elements required for RESS. A nucleotide sequence (positions -378 to -291) without any binding sites for the transcriptional activator AmyR, which is involved in amylolytic gene expression, was required for RESS. The octamer sequence TCACGGGC (positions -307 to -300) constituted the core sequence of the upstream activating element essential for amyB down-regulation under secretion stress. Both the inactivation of AmyR and RESS contributed to the down-regulation of amyB expression under ER stress.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Zhou B,Wang C,Wang B,Li X,Xiao J,Pan Ldoi
10.1007/s10529-014-1691-2subject
Has Abstractpub_date
2015-02-01 00:00:00pages
333-41issue
2eissn
0141-5492issn
1573-6776journal_volume
37pub_type
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