Abstract:
OBJECTIVES:To evaluate the effects of the surface modification of 316L stainless steel (SS) by low-temperature plasma nitriding on endothelial cells for stent applications. RESULTS:X-ray diffraction (XRD) confirmed the incorporation of nitrogen into the treated steel. The surface treatment significantly increased SS roughness and hydrophilic characteristics. After 4 h the cells adhered to the nitride surfaces and formed clusters. During the 24 h incubation period, cell viability on the nitrided surface was higher compared to the polished surface. Nitriding reduced late apoptosis of rabbit aorta endothelial cell (RAEC) on the SS surface. CONCLUSION:Low temperature plasma nitriding improved the biocompatible of stainless steel for use in stents.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Braz JKFS,Martins GM,Sabino V,Vitoriano JO,Barboza CAG,Soares AKMC,Rocha HAO,Oliveira MF,Alves Júnior C,Moura CEBdoi
10.1007/s10529-019-02657-7subject
Has Abstractpub_date
2019-05-01 00:00:00pages
503-510issue
4-5eissn
0141-5492issn
1573-6776pii
10.1007/s10529-019-02657-7journal_volume
41pub_type
杂志文章abstract::Efforts to elucidate the relationships between microorganisms and metal corrosion were mainly directed to understanding the formation of biofilm structures grown on corroded surfaces. The emergence of high throughput DNA sequencing techniques has helped in the description of microbial species involved directly and ind...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-019-02789-w
更新日期:2020-03-01 00:00:00
abstract::Dodecanol (1% v/v) and dodecanoic acid (1% w/v) inhibited growth of Yarrowia lipolytica in complex media supplemented with glucose but dodecanedioic acid (1% w/v) was not toxic. Dodecanol-tolerant strains were prepared from the wild type strain H222 as well as the acyl-CoA oxidase deleted (deltaPOX2, POX3, POX5) strai...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000025890.97067.98
更新日期:2004-05-01 00:00:00
abstract::We have cloned a novel lipase gene, lip2, from Aspergillus niger and expressed it in Escherichia coli. Upon purification of the recombinant Lip2 protein, its properties were characterized. In comparison with a previously identified lipase Lip1, both enzymes are acid lipases (optimal pH <6.5), Ca(2+)-dependent and PMSF...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0238-4
更新日期:2010-07-01 00:00:00
abstract::Promoters belong to the most important regulatory domains in genomic sequences. A novel cloning method is described to produce various DNA fragments with the average size about 100 bp, which differ by 1 bp, for screening of the osmotic pressure-inducible promoters from the genome of Escherichia coli. An osmotic pressu...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9583-3
更新日期:2008-04-01 00:00:00
abstract::Enzymatic polymerization can offer metal-free routes to polymer materials that could be used in biomedical applications. To take advantage of the unique properties of ionic liquids (ILs) for enzyme stability, monocationic ionic liquid (MIL) and dicationic ionic liquid (DIL) were used to promote the ring-opening polyme...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1160-3
更新日期:2013-06-01 00:00:00
abstract::The nitrilase from Rhodococcus sp. CCZU10-1 catalyses the hydrolysis of dinitriles to acids without the formation of amides and cyanocarboxylic acids. It was induced by benzonitrile and its analogues (tetrachloroterephthalonitrile > ε-caprolactam > benzonitrile > phenylacetonitrile), and had activity towards aromatic ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1367-3
更新日期:2014-02-01 00:00:00
abstract::Many enzymes used as digestive aids exhibit, at best, moderate stability when incubated under gastrointestinal conditions. A supplemental β-galactosidase administered orally to treat lactose intolerance was conjugated to 40 kDa, branched polyethylene glycol (PEG). PEGylation increased the enzyme's relative activity at...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0474-7
更新日期:2011-03-01 00:00:00
abstract::Two versions of an optimized gene that encodes human papilloma virus type 16 major protein L1 were designed according to the codon usage frequency of Pichia pastoris. Y16 was highly expressed in both P. pastoris and Hansenula polymorpha. M16 expression was as efficient as that of Y16 in P. pastoris, but merely detecta...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1455-z
更新日期:2014-05-01 00:00:00
abstract::Anthocyanic vacuolar inclusions (AVIs) appear as dark red-to-purple spheres of various sizes in vacuoles of grapevine (Vitis vinifera L.) cell suspension culture due to their interaction with anthocyanins. AVIs were purified and the bound anthocyanins extracted and analysed by HPLC from two lines of V. vinifera isolat...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024028603089
更新日期:2003-06-01 00:00:00
abstract::Previously, we constructed a genetically modified luciferase of Photinus pyralis that generates more than 10-fold higher luminescence intensity than the wild-type enzyme. In this study, we demonstrate that this modified luciferase enables us to detect ATP at 10(-18) mol, which is almost equal to the quantity contained...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9643-3
更新日期:2008-06-01 00:00:00
abstract::An acoustic cell settler (ACS) using ultrasound at cells of 3 MHz was used to recycle Saccharomyces cerevisiae in a fermenter. The locations of both the inlet and outlet in the acoustic cell settler, which have a relatively long distance between the transducer and reflector, were optimized. A tilted settler was design...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022345508979
更新日期:2003-02-01 00:00:00
abstract::The genes involved in the biosynthetic pathway of ectoine (2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid) from Bacillus halodurans were cloned as an operon and expressed in E. coli. Analysis of the deduced ectoine biosynthesis cluster amino acid sequence revealed that the ectoine operon contain 2,389 bp, enc...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9688-3
更新日期:2008-08-01 00:00:00
abstract::Genomic DNA was isolated from as little as 2 mg dry biomass of Magnaporthe grisea by microwave treatment within 30 s. The quantity of DNA was good enough for PCR analysis and Dot blot hybridization. This technique can be used for various studies, such as DNA fingerprinting to study the population structure of the phyt...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000003990.27624.04
更新日期:2003-11-01 00:00:00
abstract::Tandem affinity purification (TAP) is a methodology for the isolation of protein complexes from endogenous sources. It involves incorporation of a dual-affinity tag into the protein of interest and introduction of the construct into desired cell lines or organisms. Using the two affinity handles, the protein complex a...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-011-0592-x
更新日期:2011-08-01 00:00:00
abstract::Salinispora arenicola CNS-205 was a first-isolated obligate marine actinomycete. A gene (sare0357), annotated as ''amino acid adenylation domain'' located on the genome of Salinispora arenicola CNS-205, was cloned and characterized. The recombinant target protein Sare0357 was expressed in E. coli. Sare0357 specificall...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1766-8
更新日期:2015-05-01 00:00:00
abstract::Glucosylceramide and galactosylceramide were detected in three Aspergillus species: Aspergillus oryzae, Aspergillus sojae and Aspergillus. awamori, using borate-coated TLC. The cerebrosides from A. oryzae were further purified by ion exchange and iatrobeads column chromatographies with or without borate, and determine...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1631-1
更新日期:2014-12-01 00:00:00
abstract::The rpiB gene, encoding ribose-5-phosphate isomerase (RpiB) from Clostridium thermocellum, was cloned and expressed in Escherichia coli. RpiB converted D-psicose into D-allose but it did not convert D-xylose, L-rhamnose, D-altrose or D-galactose. The production of D-allose by RpiB was maximal at pH 7.5 and 65 degrees ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9393-7
更新日期:2007-09-01 00:00:00
abstract::Deer antlers are the only mammalian appendages capable of repeated rounds of regeneration. Every year, deer antlers are shed and regrown from blastema into large branched structures of cartilage and bone. Little is known about the genes involved in antler development particularly during the later stages of ossificatio...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0841-z
更新日期:2012-05-01 00:00:00
abstract::The open reading frame of the Δ(6)-desaturase gene was isolated from Mortierella alpina W15 and the gene was cloned into a pPIC3.5K vector. The vector was transformed into Pichia pastoris GS115 and expression was induced with methanol. The Δ(6)-desaturase expressed in P. pastoris GS115 catalyzed the conversion of lino...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0650-4
更新日期:2011-10-01 00:00:00
abstract::Ethanol, added as a de-emulsifier to separate oil and biocatalyst (or bacterial cells) from a three-phase (oil/biocatalyst/aqueous phase) emulsion, formed in diesel biodesulfurization employing Gordonia nitida, improved oil recovery by centrifugation from about 50% in its absence to almost 100% at 3% (v/v). The biocat...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1021750719337
更新日期:2003-01-01 00:00:00
abstract::Organophosphorus hydrolase (OPH) hydrolyzes organophosphorus esters. We constructed the yeast-displayed OPH using Flo1p anchor system. In this system, the N-terminal region of the protein was fused to Flo1p and the fusion protein was displayed on the cell surface. Hydrolytic reactions with paraoxon were carried out du...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0204-1
更新日期:2010-05-01 00:00:00
abstract::The production of biodiesel by transesterification employing acid or base catalyst has been industrially accepted for its high conversion and reaction rates. Downstream processing costs and environmental problems associated with biodiesel production and byproducts recovery have led to the search for alternative produc...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-010-0275-z
更新日期:2010-08-01 00:00:00
abstract::The developmental ability among embryos produced by three different techniques were examined: there were no significant differences in the developmental rate in porcine embryos produced by in vitro fertilization (IVF) and first generation of somatic cell nucleus transfer (SCNT), but the developmental rate dropped shar...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1467-8
更新日期:2014-06-01 00:00:00
abstract::Chloroperoxidase from Caldariomyces fumago catalyses the oxidation of 1,2-dihydronaphthalene to (1R,2R)-(+)-dihydroxytetrahydronaphthalene in homogenous citrate buffer/ionic liquid mixtures, using t-butyl hydroperoxide as O2 donor. It tolerates up to 30 (v/v) 1,3-dimethylimidazolium methylsulfate or 1-butyl-3-methylim...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-5087-6
更新日期:2004-12-01 00:00:00
abstract::Antibody drug conjugates (ADCs) have emerged as a viable option in targeted delivery of highly potent cytotoxic drugs in treatment of solid tumors. At the time of writing, only two ADCs have received regulatory approval with >40 others in clinical development. The first generation ADCs suffered from a lack of specific...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-016-2160-x
更新日期:2016-10-01 00:00:00
abstract::The gene encoding Schwanniomyces occidentalis alpha-amylase (AMY) was introduced into the chromosomal delta sequences of an industrial strain of Saccharomyces cerevisiae. To obtain a strain suitable for commercial use, an delta-integrative cassette devoid of bacterial DNA sequences was constructed that contains the AM...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1026281627466
更新日期:2003-11-01 00:00:00
abstract::The protective effects of polymer additives, including a group of viscosity-enhancing polymer poly-γ-glutamic acid (γPGA; 10, 50, and 500 kDa) and surface-active polymer Pluronic F68, on Chinese hamster ovary cells against damage due to shear stress were investigated in shake-flask cultures. The level of protection wa...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0982-8
更新日期:2012-10-01 00:00:00
abstract::To control the genetic quality during the whole process of tissue culture of the traditional Chinese medicinal plant, Saussurea involucrate Kar. et Kir., DNA polymorphisms and genetic variations were investigated using randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers. The gene...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-9984-6
更新日期:2009-08-01 00:00:00
abstract::A computational search was carried out to identify additional binding sites for the manganese response regulator, ManR, in the genome of Anabaena sp. PCC 7120. This approach predicted ManR binding sites: the promoter regions of the genes of all3575-alr3576 and the gene of alr5134 from Anabaena sp. PCC 7120. Electropho...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/B:BILE.0000045639.26579.6f
更新日期:2004-09-01 00:00:00
abstract::Microbial consortia obtained from soil samples of gasoline-polluted sites were individually enriched with pentane, hexane, isooctane and toluene. Cometabolism with methyl tert-butyl ether, (MTBE), gave maximum degradation rates of 49, 12, 32 and 0 mg g(-1)protein h(-1), respectively. MTBE was fully degraded even when ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000015424.04776.55
更新日期:2004-02-01 00:00:00