Abstract：
:The developmental ability among embryos produced by three different techniques were examined: there were no significant differences in the developmental rate in porcine embryos produced by in vitro fertilization (IVF) and first generation of somatic cell nucleus transfer (SCNT), but the developmental rate dropped sharply at the 2- to four-cell stage in recloned (second generation of SCNT) embryos. In most recloned embryos, Oct4 and Klf4 were under-expressed at all stages, whereas Sox2 and Nanog were over-expressed at the two-cell stage. In contrast, Nanog was absent in IVF and SCNT embryos at the two-cell stage. The recloned embryos were treated with valproic acid to enhance developmental capacity and this led to an increase in the rate of blastocyst formation and total cell number compared with the findings for untreated recloned embryos (29.8 vs. 12.4 %, 39 vs. 25, respectively, p < 0.05).
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Huang Y,Xie W,Yao C,Han Y,Tan G,Zhou Y,Zhu J,Pang D,Li Z,Tang Xdoi
10.1007/s10529-014-1467-8subject
Has Abstractpub_date
2014-06-01 00:00:00pages
1161-9issue
6eissn
0141-5492issn
1573-6776journal_volume
36pub_type
杂志文章abstract：:Avian myeloblastosis virus reverse transcriptase (AMV RT) is a heterodimer consisting of a 63 kDa α-subunit and a 95 kDa β subunit. Moloney murine leukaemia virus reverse transcriptase (MMLV RT) is a 75 kDa monomer. These two RTs are the most extensively used for conversion of RNA to DNA. We previously developed sever...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-012-0904-9
更新日期：2012-07-01 00:00:00
abstract：:Recombinant coagulation factor VII (FVII) is used as a potential therapeutic intervention in hemophilia patients who produce antibodies against the coagulation factors. Mammalian cell lines provide low levels of expression, however, the Spodoptera frugiperda Sf9 cell line and baculovirus expression system are powerful...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-010-0227-7
更新日期：2010-06-01 00:00:00
abstract：:There is a need for a deeper understanding of the biochemical events affecting embryonic stem (ES) cell culture by analyzing the expansion of mouse ES cells in terms of both cell growth and metabolic kinetics. The influence of the initial cell density on cell expansion was assessed. Concomitantly, the biochemical prof...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-009-0108-0
更新日期：2010-01-01 00:00:00
abstract：:The production of biodiesel by transesterification employing acid or base catalyst has been industrially accepted for its high conversion and reaction rates. Downstream processing costs and environmental problems associated with biodiesel production and byproducts recovery have led to the search for alternative produc...
journal_title：Biotechnology letters
pub_type： 杂志文章,评审
doi：10.1007/s10529-010-0275-z
更新日期：2010-08-01 00:00:00
abstract：:Genomic DNA was isolated from as little as 2 mg dry biomass of Magnaporthe grisea by microwave treatment within 30 s. The quantity of DNA was good enough for PCR analysis and Dot blot hybridization. This technique can be used for various studies, such as DNA fingerprinting to study the population structure of the phyt...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1023/b:bile.0000003990.27624.04
更新日期：2003-11-01 00:00:00
abstract：:An aqueous-organic biphasic system was established and used with whole cells of Oenococcus oeni to reduce 2-octanone to (R)-2-octanol. The conversion reached 99% when the Tris/borate buffer was increased from 50 mM to 300 mM in the aqueous phase. In addition, the conversion increased as the log P value of the organic ...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-006-9062-2
更新日期：2006-07-01 00:00:00
abstract：:A novel glucose-sensing molecule was created based on galactose/glucose-binding protein (GGBP). GGBP mutants at Asp14, a residue interacting with the 4th hydroxyl group of the sugar molecule, were constructed by mutagenesis to improve the ligand specificity of GGBP. The autofluorescence-based analysis of the binding a...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-008-9712-7
更新日期：2008-08-01 00:00:00
abstract：OBJECTIVE:To investigate the aerotolerance of Lactobacillus rhamnosus hsryfm 1301 and its influencing factors. RESULTS:The growth rate of L. rhamnosus hsryfm 1301 weakened noticeably when the concentration of supplemented H2O2 reached 1 mM, and only 2% of all L. rhamnosus hsryfm 1301 cells survived in MRS broth supple...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-018-2523-6
更新日期：2018-04-01 00:00:00
abstract：:The lipase-catalyzed reaction is useful to obtain sugar esters with chemically defined structures and will contribute to the synthesis of sugar-based compounds by a chemo-enzymatic pathway. The synthesis of sugar esters in nonaqueous media has been attempted for a quarter century. To facilitate the reactions, they hav...
journal_title：Biotechnology letters
pub_type： 杂志文章,评审
doi：10.1007/s10529-011-0663-z
更新日期：2011-10-01 00:00:00
abstract：:Excretion of 1,3-propanediol (1,3-PD) by K. pneumoniae was compared in ammonium- and phosphate-limited chemostat cultures running with an excess of glycerol. 59 and 43% catabolic flux were directed to 1,3-PD in ammonia-limited cultures and phosphate-limited cultures at dilution rate of 0.1 h(-1), respectively. Ammonia...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-009-0150-y
更新日期：2010-02-01 00:00:00
abstract：:A gene encoding a gamma-butyrolactone autoregulator receptor was cloned in to E. coli from Streptomyces ambofaciens producing spiramycin, a macrolide antibiotic used in both veterinary medicine and human medicine. A 714-bp intact receptor gene (saaR) was obtained by PCR and genomic Southern hybridization with the 100-...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-007-9613-1
更新日期：2008-05-01 00:00:00
abstract：:Several alkyl-substituted methoxysilanes were evaluated as potential activity and stability enhancing agents for biomimetic silicification of Rhodosporidium toruloides D-amino acid oxidase (RtDAO). When methyl-substituted silanes along with tetramethoxysilane were used as silicic acid precursors for polyallylamine (PA...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-012-0924-5
更新日期：2012-08-01 00:00:00
abstract：:Ultraviolet-B (UV-B) radiation has significantly negative effect on cell survival rate (P < 0.01) and positive effect on astaxanthin accumulation (P < 0.01) of Haematococcus pluvialis. H. pluvialis accumulated 3.2 mg/g of astaxanthin when being exposed to 5 W/m(2) of UV-B for 60 min prior to 72 h of high light treatme...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-010-0371-0
更新日期：2010-12-01 00:00:00
abstract：:The genetics governing the morphological switch from round or ovoid cells to filamentous growth in Saccharomyces cerevisiae has received significant interest in relation to sensing and signaling pathways as well as the control of cell processes including budding, elongation and adhesion. Little is known about the envi...
journal_title：Biotechnology letters
pub_type： 杂志文章,评审
doi：10.1007/s10529-008-9681-x
更新日期：2008-07-01 00:00:00
abstract：:A new technique for the direct production of recombinant baculovirus in the silkworm larvae is described. To assess the utility of this method, a combination of Bombyx mori nucleopolyhedroviral genome, transfer vector and Lipofectin was co-injected directly into newly ecdysed fifth instar, silkworm larvae. The recombi...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-006-9215-3
更新日期：2007-01-01 00:00:00
abstract：:Salt stress is considered one of the main abiotic factors to limit crop growth and productivity by affecting morpho-physiological and biochemical processes. Genetically, a number of salt tolerant Brassica varieties have been developed and introduced, but breeding of such varieties is time consuming. Therefore, current...
journal_title：Biotechnology letters
pub_type： 杂志文章,评审
doi：10.1007/s10529-018-2570-z
更新日期：2018-08-01 00:00:00
abstract：:A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amy...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-005-5794-7
更新日期：2005-06-01 00:00:00
abstract：OBJECTIVE:To make the previously developed biosynthesis of L-2-aminobutyric acid (L-ABA) more suitable for the industrial-scale production. RESULTS:A recyclable biotransformation system was developed based on immobilized enzyme technology. The conversion yield of L-threonine (at 90 g l(-1)) reached 99.9 % and the theo...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-015-1957-3
更新日期：2016-01-01 00:00:00
abstract：:A sigma 70-like gene, sigA, has been identified from Corynebacterium ammoniagenes. The sigA gene encodes a polypeptide of 467 amino acids with a calculated molecular mass of 52036 Da. The deduced amino acid sequence preserves the common motifs of the primary sigma factors and shows very high similarity to those of Sig...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1023/b:bile.0000021962.16204.08
更新日期：2004-04-01 00:00:00
abstract：:Many enzymes used as digestive aids exhibit, at best, moderate stability when incubated under gastrointestinal conditions. A supplemental β-galactosidase administered orally to treat lactose intolerance was conjugated to 40 kDa, branched polyethylene glycol (PEG). PEGylation increased the enzyme's relative activity at...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-010-0474-7
更新日期：2011-03-01 00:00:00
abstract：:Coupling lactate fermentation with cell growth was investigated in shake-flask and bioreactor cultivation systems by increasing aeration to improve lactate productivity in Escherichia coli CICIM B0013-070 (ackA pta pps pflB dld poxB adhE frdA). In shake-flasks, cells reached 1 g dry wt/l then, cultivated at 100 rpm an...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-012-0883-x
更新日期：2012-06-01 00:00:00
abstract：OBJECTIVE:Heterotrophic manganese (Mn)-oxidizing microorganisms responsible for biogenic manganese oxide (Bio-MnOx) production are fastidious. Their enrichment is not easily accomplished by merely adding a soluble organic substrate to non-sterile mixed cultures. The objective of this study was to evaluate polycaprolact...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-021-03088-z
更新日期：2021-01-26 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-007-9463-x
更新日期：2007-11-01 00:00:00
abstract：:Characterization of cry gene contents can help to predict the insecticidal activities of Bacillus thuringiensis isolates and in the searching of new cry genes. PCR-Single-strand conformation polymorphism (SSCP) profiling and sequencing of the highly variable cry gene regions were used to characterize cry gene content ...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-006-9224-2
更新日期：2007-02-01 00:00:00
abstract：:The ability of Aspergillus strains to utilize thin stillage to produce malic acid was compared. The highest malic acid was produced by Aspergillus niger ATCC 9142 at 17 g l(-1). Biomass production from thin stillage was similar with all strains but ATCC 10577 was the highest at 19 g l(-1). The highest malic acid yield...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-011-0720-7
更新日期：2011-12-01 00:00:00
abstract：:The lipase of Pseudomonas cepacia was immobilized onto electrospun polyacrylonitrile (PAN) fibers and used for the conversion of (S)-glycidol with vinyl n-butyrate to glycidyl n-butyrate in isooctane. The rate of reaction with the adsorbed lipase was 23-fold higher than the initial material. After 10 recyclings, the i...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-010-0279-8
更新日期：2010-08-01 00:00:00
abstract：:Cell-free extracts of Streptomyces sp. DRS I converted ampicillin to cephalexin, presumably due to the activity of the enzyme, expandase. The extract was fractionated and characterized by colorimetric and chromatographic measurements coupled with disc-agar diffusion bioassay against an ampicillin-resistant, cephalexin...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-009-9973-9
更新日期：2009-07-01 00:00:00
abstract：:Among single-site mutations of L-arabinose isomerase derived from Geobacillus thermodenitrificans, two mutants were produced having the lowest and highest activities of D-tagatose production. Site-directed mutagenesis at these sites showed that the aromatic ring at amino acid 164 and the size of amino acid 475 were im...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-005-5327-4
更新日期：2006-02-01 00:00:00
abstract：OBJECTIVE:Microalgae gained interest for potential use as biodiesel producers, since they synthesize and accumulate significant quantities of lipids. The aim of this work was to isolate indigenous microalgae strains from Greek habitats, study their physicochemical growth conditions and finally select the best ones with...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-019-02658-6
更新日期：2019-05-01 00:00:00
abstract：OBJECTIVES:To examine the synergistic antibacterial activity of violacein and silver nanoparticles (AgNPs) against ATCC bacteria, Staphylococcus aureus, Escherichia coli and two bacteria isolated from bovine mastitis. METHODS:Violacein from Chromobacterium violaceum and biogenic AgNPs from Fusarium oxysporum were eval...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-019-02745-8
更新日期：2019-12-01 00:00:00
