Characterization of the highly variable cry gene regions of Bacillus thuringiensis strain ly4a3 by PCR-SSCP profiling and sequencing.


:Characterization of cry gene contents can help to predict the insecticidal activities of Bacillus thuringiensis isolates and in the searching of new cry genes. PCR-Single-strand conformation polymorphism (SSCP) profiling and sequencing of the highly variable cry gene regions were used to characterize cry gene content of B. thuringiensis strain ly4a3. The highly variable regions with about 1100 bp in sizes were amplified using a degenerate primer pair for cry genes, OL2(d) and OL5(r). A library of the PCR product was constructed, and all white colonies were subjected to PCR using another degenerate primer pair for cry genes, OL3(d) and OL5(r), with products about 250 bp in sizes. Two different profiles were observed based on SSCP profiling for the PCR products. The cry genes in the two corresponding colonies were sequenced and their deduced amino acids showed high identities to Cry1Ab (84.5% approximately 98.4%) and Cry1I (88.78% approximately 98.4%), respectively. This method allows the quick characterization of cry gene content of B. thuringiensis isolates and the detection of new cry genes.


Biotechnol Lett


Biotechnology letters


Lin Y,Fang G,Peng K




Has Abstract


2007-02-01 00:00:00












  • Assessment of genetic stability in tissue-cultured products and seedlings of Saussurea involucrata by RAPD and ISSR markers.

    abstract::To control the genetic quality during the whole process of tissue culture of the traditional Chinese medicinal plant, Saussurea involucrate Kar. et Kir., DNA polymorphisms and genetic variations were investigated using randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers. The gene...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Yuan XF,Dai ZH,Wang XD,Zhao B

    更新日期:2009-08-01 00:00:00

  • High-level production of α-amylase by manipulating the expression of alanine racamase in Bacillus licheniformis.

    abstract:OBJECTIVES:To improve target protein production by manipulating expression levels of alanine racemase in Bacillus licheniformis. RESULTS:The gene of dal was identified to be responsible for alanine racemase function. Based on the selection marker of dal, a food-grade expression system was constructed in B. licheniform...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: He P,Zhang Z,Cai D,Chen Y,Wang H,Wei X,Li S,Chen S

    更新日期:2017-09-01 00:00:00

  • Well-defined differentiation of hESC-derived hemangioblasts by embryoid body formation without enzymatic treatment.

    abstract::Human hemangioblasts exist only during the early embryonic developmental stage thereby limiting the adult cellular source from which to obtain such cells for study. To overcome this, hemangioblast studies have focused on utilizing human embryonic stem cell (hESC) derivatives but current methods are cell-line dependent...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Gil CH,Lee JH,Seo J,Park SJ,Park Z,Kim J,Jung AR,Lee WY,Kim JS,Moon SH,Lee HT,Chung HM

    更新日期:2015-06-01 00:00:00

  • Autodisplay of an avidin with biotin-binding activity on the surface of Escherichia coli.

    abstract:OBJECTIVES:To display a recombinant avidin fused to the autotransporter ShdA to bind biotinylated molecules on the surface of Escherichia coli. RESULTS:Two chimeric protein constructs containing avidin fused to the autotransporter ShdA were expressed on the surface of Escherichia coli DH5α. One fusion protein containe...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Pardavé-Alejandre HD,Alvarado-Yaah JE,Pompa-Mera EN,Muñoz-Medina JE,Sárquiz-Martínez B,Santacruz-Tinoco CE,Manning-Cela RG,Ortíz-Navarrete V,López-Macías C,González-Bonilla CR

    更新日期:2018-03-01 00:00:00

  • Involvement of polyamines in plant response to abiotic stress.

    abstract::Environmental stresses are the major cause of crop loss worldwide. Polyamines are involved in plant stress responses. However, the precise role(s) of polyamine metabolism in these processes remain ill-defined. Transgenic approaches demonstrate that polyamines play essential roles in stress tolerance and open up the po...

    journal_title:Biotechnology letters

    pub_type: 杂志文章,评审


    authors: Alcázar R,Marco F,Cuevas JC,Patron M,Ferrando A,Carrasco P,Tiburcio AF,Altabella T

    更新日期:2006-12-01 00:00:00

  • Screening and identification of a specific peptide binding to breast cancer cells from a phage-displayed peptide library.

    abstract:OBJECTIVES:Breast cancer is a popular fatal malignant tumor for women with high of rates incidence and mortality. Development of the new approaches for breast cancer targeted diagnosis and chemotherapy is emergently needed by the current clinical practice, the important first step is finding a breast cancer specificall...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Jin H,Gao X,Xiao L,He H,Cheng S,Zhang C,Hou Y,Song F,Su X,Gao Q,Lu Z,Yang R,Song X,Yang J,Duan W,Hou Y

    更新日期:2021-01-01 00:00:00

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    abstract::There has been an increasing interest in applying biotechnology in formulating and characterising new and innovative drug delivery methods, e.g., drug-loaded biodegradable microneedles within the area of transdermal delivery technology. Recently, microneedles have been proposed for use in pain management, e.g., post-o...

    journal_title:Biotechnology letters

    pub_type: 杂志文章,评审


    authors: Nayak A,Das DB

    更新日期:2013-09-01 00:00:00

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    abstract::Alkyl glucosides were synthesized by the reaction of Leuconostoc mesenteroides dextransucrase with sucrose and various alcohols. Alkyl alpha-D-glucosides were obtained with a yield of 30% (mol/mol) with primary alcohols, but secondary alcohols or tertiary alcohols gave yields below 5%. The optimal yield was 50% using ...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Kim YM,Kim BH,Ahn JS,Kim GE,Jin SD,Nguyen TH,Kim D

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  • Novel biotechnology approaches in colorectal cancer diagnosis and therapy.

    abstract::With ever-increasing molecular information about colorectal cancer (CRC), there is an expectation to detect more sensitive and specific molecular markers for new advanced diagnostic methods that can surpass the limitations of current screening tests. Moreover, enhanced molecular pathology knowledge about cancer has le...

    journal_title:Biotechnology letters

    pub_type: 杂志文章,评审


    authors: Kavousipour S,Khademi F,Zamani M,Vakili B,Mokarram P

    更新日期:2017-06-01 00:00:00

  • Augmenting the expression of accD and rbcL genes using optimized iron concentration to achieve higher biomass and biodiesel in Chlorella vulgaris.

    abstract::Chlorella vulgaris is a form of microalgae commonly employed as a biological source of oil for biodiesel production. Major algal cultivation strategies are focused on stimulating growth rate and lipid content. In the present study, the algal growth media was supplemented with iron (III) chloride (FeCl3), as a stimulat...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Khamoushi A,Tafakori V,Zahed MA,Gayglou SE,Angaji SA

    更新日期:2020-12-01 00:00:00

  • Functional expression of the astaxanthin biosynthesis genes from a marine bacterium, Paracoccus haeundaensis.

    abstract::The astaxanthin biosynthesis gene cluster in Paracoccus haeundaensis consists of six genes: crtW, crtZ, crtY, crtI, crtB, and crtE contain 726, 486, 1158, 1503, 912, and 879 base pairs, respectively. Individual carotenoid biosynthesis genes of P. haeundaensis have now been expressed in E. coli and each gene product ha...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Lee JH,Kim YT

    更新日期:2006-08-01 00:00:00

  • Optimization of covalent immobilization of pectinase on sodium alginate support.

    abstract::Pectinase was immobilized on a sodium alginate support using glutaraldehyde and retained 66% activity. The optimal pH for activity shifted from 3.0 to 3.5 after immobilization; however, the optimum temperature remained unchanged at 40 degrees C. The immobilized enzyme also had a higher thermal stability and reusabilit...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Li T,Wang N,Li S,Zhao Q,Guo M,Zhang C

    更新日期:2007-09-01 00:00:00

  • Molecular cloning and characterization of the α-chain gene of goose immunoglobulin heavy chain.

    abstract::A novel gene encoding the α-chain of goose immunoglobulin heavy-chain (Igα) was cloned by reverse transcription-PCR. The cDNA had 1,760 bp and encompassed a partial V-D-J region of the heavy chain, a constant region (Cα) and 3'-untranslated region of α-chain. The Cα gene contains four constant region domains (CH1-CH4)...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Guo Y,Gao M,Luo X,Sheng Q,Ma B,Wang J

    更新日期:2014-04-01 00:00:00

  • Timing of bifidobacterium administration influences the development of allergy to ovalbumin in mice.

    abstract::C3H/HeJ mice were sensitized with ovalbumin (OVA) and choleratoxin (CT) for 5 weeks, and then Bifidobacterium bifidum BGN4 was administered continuously for 7 weeks, starting 2 weeks before (pre-treatment group) and 2 weeks after (post-treatment group) the initial sensitization. After sensitization, the OVA-induced (s...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Kim H,Lee SY,Ji GE

    更新日期:2005-09-01 00:00:00

  • Prevention of osteoporosis in ovariectomized mice by electroporational gene transfer of parathyroid hormone.

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    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Liu B,Tang J,Ji J,Gu J

    更新日期:2003-12-01 00:00:00

  • Recombinant expression of sericin-cecropin fusion protein and its functional activity.

    abstract:OBJECTIVE:Silk sericin is a natural polymer with potential utility in biomedical and biotechnological applications. Recombinantly expressed sericin ensures a source of pure protein with no contamination and with multiple properties when expressed as a fusion protein. Hence, the present paper aims to recombinantly expre...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Thomas DS,Manoharan C,Rasalkar S,Mishra RK,Gopalapillai R

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    abstract::A coupled in silico thermodynamic and probabilistic metabolic control analysis methodology was verified by applying it to the glycerol biosynthetic pathway in Saccharomyces cerevisiae. The methodology allows predictions even when detailed knowledge of the enzyme kinetics is lacking. In a metabolic steady state, we fou...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Birkenmeier M,Mack M,Röder T

    更新日期:2015-02-01 00:00:00

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    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Brown AS,Calcott MJ,Collins VM,Owen JG,Ackerley DF

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  • Structural peculiarities of linear megaplasmid, pLMA1, from Micrococcus luteus interfere with pyrosequencing reads assembly.

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    authors: Wagenknecht M,Dib JR,Thürmer A,Daniel R,Farías ME,Meinhardt F

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  • Characterization of decrystallized chitosan and its application in biosorption of textile dyes.

    abstract::Decrystallized chitosan was produced from shrimp shells with a low degree of crystallinity (10%) and a high anionic dye binding capacity. Raw, mixed dye wastewater from a textile factory was efficiently decolorized using decrystallized chitosan that was more efficient than using normal chitosan and activated carbon. D...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Trung TS,Ng CH,Stevens WF

    更新日期:2003-07-01 00:00:00

  • Synthesis, expression and purification of a type of chlorotoxin-like peptide from the scorpion, Buthus martensii Karsch, and its acute toxicity analysis.

    abstract::A gene, rBmK Cta, encoding a chlorotoxin-like peptide from the scorpion, Buthus martensii Karsch, was synthesized according to the sequence optimized for codon usage in Escherichia coli and was expressed in E. coli BL21 (DE3) using a pExSecI expression system in which the IgG-binding domain-ZZ of protein A is fused to...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Fu YJ,Yin LT,Wang W,Chai BF,Liang AH

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  • Pluripotent-related gene expression analyses in single porcine recloned embryo.

    abstract::The developmental ability among embryos produced by three different techniques were examined: there were no significant differences in the developmental rate in porcine embryos produced by in vitro fertilization (IVF) and first generation of somatic cell nucleus transfer (SCNT), but the developmental rate dropped shar...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Huang Y,Xie W,Yao C,Han Y,Tan G,Zhou Y,Zhu J,Pang D,Li Z,Tang X

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  • Poly-γ-glutamic acid enhances the growth and viability of Chinese hamster ovary cells in serum-free medium.

    abstract::The protective effects of polymer additives, including a group of viscosity-enhancing polymer poly-γ-glutamic acid (γPGA; 10, 50, and 500 kDa) and surface-active polymer Pluronic F68, on Chinese hamster ovary cells against damage due to shear stress were investigated in shake-flask cultures. The level of protection wa...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Chun BH,Lee YK,Chung N

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    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Takada M,Nomura N,Okada H,Nakajima-Kambe T,Nakahara T,Uchiyama H

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  • Significant differences in integration sites of Moloney murine leukemia virus/Moloney murine sarcoma virus retroviral vector carrying recombinant coagulation factor IX in two human cell lines.

    abstract::Ligation-mediated-PCR was performed followed by the mapping of 177 and 150 integration sites from HepG2 and Hek293 transduced with chimera vector carrying recombinant human Factor IX (rhFIX) cDNA, respectively. The sequences were analyzed for chromosome preference, CpG, transcription start site (TSS), repetitive eleme...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Castilho-Fernandes A,Fontes AM,Abraham KJ,de Freitas MC,da Rosa NG,Picanço-Castro V,de Sousa Russo-Carbolante EM,Covas DT

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  • Production of biologically active IgG hinge-tag soluble epidermal growth factor receptors (ErbB).

    abstract::The extracellular domains (ECD) of epidermal growth factor receptors, ErbB1, 2, 3 and 4, were designed as soluble dimeric forms. Each ECD was fused to a short hinge region derived from IgG, such that the stable dimer could be formed with disulfide bridges. This hinge-tagged design minimized the molecular weight to app...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Otani T,Hashizume T,Nagaoka T,Fukuda T,Tang CK,Salomon DS,Seno M

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  • A simple protocol for isolation of fungal DNA.

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    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Tendulkar SR,Gupta A,Chattoo BB

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    journal_title:Biotechnology letters

    pub_type: 杂志文章


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    journal_title:Biotechnology letters

    pub_type: 杂志文章


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    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Lee KS,Kim BY,Je YH,Woo SD,Sohn HD,Jin BR

    更新日期:2007-01-01 00:00:00