Abstract:
:Brevibacterium epidermis DSM 20659 is a halotolerant Gram-positive bacterium which can synthesize the osmolyte, ectoine, but prefers to take it up from its environment. The present study revealed that B. epidermis is equipped with at least one transport system for ectoine, with a maximal transport velocity of 15.7 +/- 4.3 nmol/g CDW.min. The transport requires energy (ATP) and is completely inhibited by the proton uncoupler, CCCP. The ectoine uptake system is constitutively expressed at a basal level of activity and its activity is immediately 10-fold increased by hyper-osmotic stress. Initial uptake rates are not influenced by the intensity of the hyper-osmotic shock but the duration of the increased activity of the uptake system could be directly related to the osmotic strength of the assay solution. Competition assays indicate that betaine, but not proline, is also transported by the ectoine uptake system.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Onraedt A,De Mey M,Walcarius B,Soetaert W,Vandamme EJdoi
10.1007/s10529-006-9149-9subject
Has Abstractpub_date
2006-11-01 00:00:00pages
1741-7issue
21eissn
0141-5492issn
1573-6776journal_volume
28pub_type
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pub_type: 杂志文章
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doi:10.1023/b:bile.0000025881.95596.23
更新日期:2004-05-01 00:00:00
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pub_type: 信件
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更新日期:2014-06-01 00:00:00