Abstract:
OBJECTIVE:To develop a mouse artificial chromosome (MAC) carrying the mouse Xist gene (X-inactive specific transcript; Xist-MAC) as a systematic in vitro approach for investigating Xist RNA-mediated chromosome inactivation. RESULTS:Ectopic expression of the Xist gene in CHO cells led to the accumulation of Xist RNA in cis on the MAC. In addition, the introduction of Xist-MAC to embryonic stem cells from male mice via microcell-mediated chromosome transfer resulted in the accumulation of Xist RNA in cis on the MAC. Chromosomal inactivation was observed in the differentiated state. Moreover, this phenomenon was accompanied by the epigenetic modification of H3K27 trimethylation. CONCLUSIONS:We successfully generated a novel chromosome inactivation model, Xist-MAC, which will provide a valuable tool for the screening and functional analysis of X chromosome inactivation-related genes and proteins.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Inaoka D,Sunamura N,Ohira T,Nakayama Y,Kugoh Hdoi
10.1007/s10529-020-02826-zsubject
Has Abstractpub_date
2020-05-01 00:00:00pages
697-705issue
5eissn
0141-5492issn
1573-6776pii
10.1007/s10529-020-02826-zjournal_volume
42pub_type
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journal_title:Biotechnology letters
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2008-02-01 00:00:00