Abstract:
:The transfer of the IncP-7 carbazole degradative plasmid pCAR1 from Pseudomonas putida SM1443 (derived from strain KT2440) into bacteria of river water samples was monitored using a reporter gene encoding red fluorescent protein (RFP). The number of transconjugants drastically increased in the presence of carbazole, and most appeared to belong to the genus Pseudomonas. The results suggest that the presence of carbazole benefits the appearance of transconjugants belonging to the genus Pseudomonas. Intriguingly, we also detected the transfer of pCAR1 into non-Pseudomonas, Stenotrophomonas-like bacteria.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Shintani M,Fukushima N,Tezuka M,Yamane H,Nojiri Hdoi
10.1007/s10529-007-9519-ysubject
Has Abstractpub_date
2008-01-01 00:00:00pages
117-22issue
1eissn
0141-5492issn
1573-6776journal_volume
30pub_type
杂志文章abstract::A brief historical perspective on metagenomics is given followed by a discussion of the rapid progress in this field largely defined by transition to the next generation sequencing technologies. Problems and challenges connected to this transition are also addressed. The review focuses on recent literature describing ...
journal_title:Biotechnology letters
pub_type: 历史文章,杂志文章,评审
doi:10.1007/s10529-010-0306-9
更新日期:2010-10-01 00:00:00
abstract:OBJECTIVES:Sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum is one of the major fungal diseases of canola. To develop resistance against this fungal disease, the chit42 from Trichoderma atroviride with chitin-binding domain and polygalacturonase-inhibiting protein 2 (PG1P2) of Phaseolus vulgaris were co-ex...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2058-7
更新日期:2016-06-01 00:00:00
abstract::Feruloylated mono- and di-acylglycerols were synthesized in a two step reaction: ethyl ferulate was first transesterified with glycerol and then this was esterified with oleic acid. The yield of the combined feruloylated mono- and di-acylglycerols in the second reaction reached 96% when esterification of 0.3 g transes...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9486-3
更新日期:2007-12-01 00:00:00
abstract::Genetically-coded, fluorescence resonance energy transfer (FRET) biosensors are widely used to study molecular events from single cells to whole organisms. They are unique among biosensors because of their spontaneous fluorescence and targeting specificity to both organelles and tissues. In this review, we discuss the...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-006-9193-5
更新日期:2006-12-01 00:00:00
abstract::Mucor circinelloides and Mortierella isabellina hydroxylated dehydroabietic acid (DehA). DehA was converted regio- and stereo-selectively by whole cells of Mr. circinelloides to give 2alpha-hydroxydehydroabietic acid in a 75% molar conversion yield (11 mM from 14.7 mM DehA) after 72 h in the cultivation medium contain...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3224-5
更新日期:2005-09-01 00:00:00
abstract::Nitrogenous derivatives of the two orange pigments from Monascus sp. with anti-melanogenic activities were prepared using fermentation and chemical synthesis. The pigments were produced in a 5 l jar fermentor. A total of 33 derivatives were synthesized via incorporation of L-amino acids and amines into the pigments. T...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1518-1
更新日期:2014-08-01 00:00:00
abstract::This review concerns basic research on cellulases and cellulose-specific carbohydrate-binding modules (CBMs). As a background, glycosyl hydrolases are also briefly reviewed. The nomenclature of cellulases and CBMs is discussed. The main cellulase-producing organisms and their cellulases are described. Synergy, enantio...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-004-4579-8
更新日期:2004-11-01 00:00:00
abstract::The exploded bast, branch and stem of cotton stalk were degraded by alkalophilic Bacillus NT-19, with weight losses of 24%, 20% and 14%, respectively, after 14 d. Compared with a white-rot fungus (Phanerochaete chrysosporium), Bacillus NT- 19 preferentially degraded the non-cellulose components of cotton stem. The rel...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1026083827968
更新日期:2003-10-01 00:00:00
abstract::An efficient method for creating a DNA library is presented in which gene mutagenesis and recombination can be introduced by integrating error-prone PCR with a staggered extension process in one test tube. In this process, less than 15 cycles of error-prone PCR are used to introduce random mutations. After precipitate...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9674-9
更新日期:2008-07-01 00:00:00
abstract::The hybridoma cell line, HB-8696, produces a monoclonal antibody, 520C9 (mouse IgG(1)) that recognizes the breast cancer oncoprotein, c-erbB2. The effect of perfusion rate (volume of fresh feed/working volume of reactor/day) on cell growth and mAb production was investigated but perfusion at a constant rate and at an ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1058-5
更新日期:2013-02-01 00:00:00
abstract::Human beta-amyloid (Abeta) is believed to be one of the main components of Alzheimer's disease, so reduction of Abeta is considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for Abeta with tandem repeats. Integration of the human Abeta gene into the...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9759-5
更新日期:2008-10-01 00:00:00
abstract::Continuous production of fructooligosaccharides (FOS) by Aureobasidium pullulans immobilized on calcium alginate beads with a packed bed was investigated at a plant scale reactor. Optimum conditions were with 770 g sucrose/l, being fed at 200 l/h at 50°C which gave a productivity of 180 g FOS/l h. Initial activity was...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0606-8
更新日期:2011-08-01 00:00:00
abstract:OBJECTIVES:To provide a simple method to make a stable ΔF508-CFTR-expressing T84 cell line that can be used as an efficient screening model system for ΔF508-CFTR rescue. RESULTS:CFTR knockout cell lines were generated by Cas9 with a single-guide RNA (sgRNA) targeting exon 1 of the CFTR genome, which produced indels th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2190-4
更新日期:2016-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9013-y
更新日期:2006-06-01 00:00:00
abstract::Cell division of the wild type strain Corynebacterium (formerly Brevibacterium) ammoniagenes ATCC 6872 which requires 1 microM Mn2+ for balanced growth was inhibited by addition of 20 mM hydroxyurea (HU) or 10 mM p-methoxyphenol (MP) to a Mn2+-supplemented fermentation medium at an appropriate time. Scanning electron ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1021919404066
更新日期:2003-01-01 00:00:00
abstract::General methods for identification of usual carotenoids including C18-HPLC retention time and spectroscopic methods, such as absorption spectra, mass spectra, NMR spectra, are briefly summarized. ...
journal_title:Biotechnology letters
pub_type: 信件
doi:10.1007/s10529-014-1479-4
更新日期:2014-06-01 00:00:00
abstract:OBJECTIVES:To identify a new member of serine proteases from Deinagkistrodon acutus via phage display technique and appraise its biocatalytic activities. RESULTS:A novel thrombin-like enzyme gene was cloned by screening the phage display library of D. acutus venom gland. The gene has a 783 bp ORF encoding 260 amino ac...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2441-z
更新日期:2018-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0443-1
更新日期:2011-02-01 00:00:00
abstract::A microtiter plate-based assay was developed to evaluate the ability of lipases to perform transesterifications when employed in different organic solvents. A 4-nitrophenol assay was carried out employing seven different lipase formulations and two fatty acid methyl esters with different chain lengths in a total of si...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1721-0
更新日期:2015-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9898-8
更新日期:2009-04-01 00:00:00
abstract::Interactions between ectomycorrhizal fungi (Suillus laricinus, S. tomentosus, Amanita vaginata and Gomphidius viscidus) and the pathogen Rhizoctonia solani in co-culture were studied using both light and scanning electron microscopy. S. laricinus, S. tomentosus and A. vaginata inhibited the growth of the pathogen. Mor...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9760-z
更新日期:2008-10-01 00:00:00
abstract::A novel method to examine the morphology and structure of fungal hyphae in solid pastes used for the production of meat alternative product is presented. A sample of fermentation broth was fluorescently stained with Calcofluor White and added back to the broth, mixed and then a paste made using ultra-filtration. Fibre...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022393116596
更新日期:2003-02-01 00:00:00
abstract::Three biological activities of 12 soy isoflavones were investigated in terms of the structure-activity relationship. Seven molecular descriptors and one absorption predictor were significantly related to the inhibition of cytochrome P4501 activity in HepG2 cells by soy isoflavones, yielding water solubility [correlati...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-8457-9
更新日期:2005-08-01 00:00:00
abstract::We prepared ATP photosynthetic vesicles from inside-out membranes of Escherichia coli cells that express delta-rhodopsin (a novel light-driven H(+) transporter) and TF(0)F(1)-ATP synthase (a thermo-stable ATP synthase). These vesicles showed light-dependent ATP synthesis. Furthermore, coupling the ATP photosynthetic v...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0544-5
更新日期:2011-06-01 00:00:00
abstract:OBJECTIVES:To optimize the production of active inclusion bodies (IBs) containing human D-amino acid oxidase (hDAAO) in Escherichia coli. RESULTS:The optimized initial codon region combined with the coexpressed rare tRNAs, fusion of each of the N-terminal partners including cellulose-binding module, thioredoxin, gluta...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2413-3
更新日期:2017-11-01 00:00:00
abstract::A morphological parameter of polygonal index was defined as the ratio of cell adhesion area versus the square of the major cell axis, and cells that had an adhesion area larger than 4000 mum(2) and a polygonal index larger than 0.3 were considered large polygonal cells. Cell morphology tended to change from fibroblast...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9683-8
更新日期:2008-07-01 00:00:00
abstract::To understand the mechanism of phosphate accumulation, a gene encoding polyphosphate kinase (PPK) was cloned from the genomic library of Serratia marcescens by Southern hybridization. From the nucleotide sequence of a 4 kb DNA fragment, an open reading frame of 2063 nucleotides was identified encoding a protein of 686...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022386514487
更新日期:2003-02-01 00:00:00
abstract::Keratinase from Pseudomonas aeruginosa KS-1 was expressed constitutively as an extracellular protein in Escherichia coli with high specific activity of 3.7 kU/mg. It was purified fourfold as a 33 kDa monomeric protein by Q-Sepharose ion exchange chromatography with a recovery of 95%. It is a serine protease with optim...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0361-2
更新日期:2010-12-01 00:00:00
abstract::Ligation-mediated-PCR was performed followed by the mapping of 177 and 150 integration sites from HepG2 and Hek293 transduced with chimera vector carrying recombinant human Factor IX (rhFIX) cDNA, respectively. The sequences were analyzed for chromosome preference, CpG, transcription start site (TSS), repetitive eleme...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1764-2
更新日期:2015-05-01 00:00:00
abstract:PURPOSE OF WORK:The biosynthetic pathway of a new antitumor compound, haloroquinone, is elucidated to facilitate metabolic regulation for product accumulation and modification to produce new bioactive structural analogues of the compound. The biosynthetic origin of a novel promising protein kinase B inh...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1019-z
更新日期:2012-11-01 00:00:00