Abstract:
:A novel method to examine the morphology and structure of fungal hyphae in solid pastes used for the production of meat alternative product is presented. A sample of fermentation broth was fluorescently stained with Calcofluor White and added back to the broth, mixed and then a paste made using ultra-filtration. Fibre visualisation was by fluorescence microscopy and quantification by manual image analysis. This method enables the determination of fibre length and orientation within the paste. Imaging of the hypha fibre paste proved that its structure was 'isotropic', i.e. that fibres were randomly oriented in all directions. Processing of the paste altered the orientation of the fibres, the method was able to identify and quantify the changes in fibre position.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Miri T,Cox PW,Fryer PJdoi
10.1023/a:1022393116596keywords:
subject
Has Abstractpub_date
2003-02-01 00:00:00pages
295-300issue
4eissn
0141-5492issn
1573-6776journal_volume
25pub_type
杂志文章abstract:OBJECTIVES:Regenerative medicine approaches using reprogrammed or transdifferentiated cells require efficient single cell expression profiling to analyze culture homogeneity for quality control and recipients' safety. RESULTS:While antigen-antibody based systems have been developed for several proteins, probing at the...
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journal_title:Biotechnology letters
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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doi:10.1007/s10529-013-1321-4
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pub_type: 杂志文章
doi:10.1007/s10529-013-1405-1
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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doi:10.1007/s10529-013-1195-5
更新日期:2013-08-01 00:00:00
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doi:10.1007/s10529-020-03040-7
更新日期:2021-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-009-0034-1
更新日期:2009-09-01 00:00:00
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doi:10.1007/s10529-015-1786-4
更新日期:2015-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2123-2
更新日期:2016-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s10529-005-0938-3
更新日期:2005-09-01 00:00:00
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doi:10.1007/s10529-019-02767-2
更新日期:2020-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s10529-017-2394-2
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更新日期:2008-12-01 00:00:00
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更新日期:2006-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2012-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2015-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1058-5
更新日期:2013-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9149-9
更新日期:2006-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-010-0275-z
更新日期:2010-08-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1123-0
更新日期:2013-04-01 00:00:00