Abstract:
:An index [kv: average isolation rate of viable protoplast (number/ml min)] was established to evaluate the optimal conditions for protoplast isolation from cultured plant cells. The optimal conditions for protoplasts isolation from Nicotiana tabacum BY2 cultured cells could be determined on the basis of the kv [31.7 x 10(3) (number/ml min)]. The colony-forming efficiency of the protoplasts was about 46%. The optimal conditions for protoplasts isolation from Catharanthus roseus [kv = 38.1 x 10(3) (number/ml min)] and Wasabia japonica [kv = 14.2 x 10(3) (number/ml min)] cultured cells could also be determined. Furthermore, a method for rapid regenerating cell wall of protoplast in liquid culture using alginate gel containing locust bean gum was developed.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Aoyagi Hdoi
10.1007/s10529-006-9140-5subject
Has Abstractpub_date
2006-10-01 00:00:00pages
1687-94issue
20eissn
0141-5492issn
1573-6776journal_volume
28pub_type
杂志文章abstract::Avian myeloblastosis virus reverse transcriptase (AMV RT) is a heterodimer consisting of a 63 kDa α-subunit and a 95 kDa β subunit. Moloney murine leukaemia virus reverse transcriptase (MMLV RT) is a 75 kDa monomer. These two RTs are the most extensively used for conversion of RNA to DNA. We previously developed sever...
journal_title:Biotechnology letters
pub_type: 杂志文章
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journal_title:Biotechnology letters
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2014-05-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000019556.79703.4b
更新日期:2004-03-01 00:00:00
abstract::Anthocyanic vacuolar inclusions (AVIs) appear as dark red-to-purple spheres of various sizes in vacuoles of grapevine (Vitis vinifera L.) cell suspension culture due to their interaction with anthocyanins. AVIs were purified and the bound anthocyanins extracted and analysed by HPLC from two lines of V. vinifera isolat...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024028603089
更新日期:2003-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000030040.13828.d7
更新日期:2004-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1735-7
更新日期:2015-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1857-6
更新日期:2015-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9769-3
更新日期:2008-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2507-6
更新日期:2018-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022890119847
更新日期:2003-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025049825720
更新日期:2003-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0227-7
更新日期:2010-06-01 00:00:00
abstract::Salinispora arenicola CNS-205 was a first-isolated obligate marine actinomycete. A gene (sare0357), annotated as ''amino acid adenylation domain'' located on the genome of Salinispora arenicola CNS-205, was cloned and characterized. The recombinant target protein Sare0357 was expressed in E. coli. Sare0357 specificall...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1766-8
更新日期:2015-05-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025464918453
更新日期:2003-09-01 00:00:00
abstract::The use of H(2), He and O(2) during batch fermentation of Saccharomyces cerevisiae BRAS291 increased the final intracellular glycogen contents of the cells from 2-fold to 10-fold compared with a gas-free condition, and this depended on the gas applied. Differently, the intracellular trehalose contents increased from 2...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9533-0
更新日期:2008-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03040-7
更新日期:2021-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2348-8
更新日期:2017-08-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2723-8
更新日期:2005-11-01 00:00:00
abstract::A coupled in silico thermodynamic and probabilistic metabolic control analysis methodology was verified by applying it to the glycerol biosynthetic pathway in Saccharomyces cerevisiae. The methodology allows predictions even when detailed knowledge of the enzyme kinetics is lacking. In a metabolic steady state, we fou...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1675-2
更新日期:2015-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0650-4
更新日期:2011-10-01 00:00:00
abstract::Aptamers against inactive Vibrio alginolyticus were selected from an 82-nt ssDNA random library by systematic evolution of ligands by exponential enrichment. After 15 rounds of selection, the final pool of aptamers was highly specific for inactivated V. alginolyticus and had a dissociation constant of 27.5 ± 9.2 nM. U...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1154-1
更新日期:2013-06-01 00:00:00
abstract::Pectinase was immobilized on a sodium alginate support using glutaraldehyde and retained 66% activity. The optimal pH for activity shifted from 3.0 to 3.5 after immobilization; however, the optimum temperature remained unchanged at 40 degrees C. The immobilized enzyme also had a higher thermal stability and reusabilit...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9409-3
更新日期:2007-09-01 00:00:00
abstract::An alginate lyase gene, algA, encoding a new poly β-D-mannuronate (polyM)-specific alginate lyase AlgA, was cloned from Pseudomonas sp. E03. The recombinant AlgA with (His)6-tag, consisting of 364 amino acids (40.4 kDa),was purified using Ni-NTA Sepharose. The purified lyase had maximal activity (222 EU/mg) at pH 8 an...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1685-0
更新日期:2015-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0504-5
更新日期:2011-04-01 00:00:00
abstract:OBJECTIVES:The aim of the study is to generate a spherical three-dimensional (3D) aggregate of hepatocyte-like cells (HLCs) differentiated from human embryonic stem cells and to investigate the effect of the 3D environment on hepatic maturation and drug metabolism. RESULTS:Quantitative real-time PCR analysis indicated...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2528-1
更新日期:2018-05-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-013-1217-3
更新日期:2013-09-01 00:00:00
abstract::Chlorella protothecoides, a lutein-producing microalga, was grown aerobically in a mineral medium prepared with 70% (v/v) deuterated water. HPLC/atmospheric pressure chemical ionization-mass spectrometry (HPLC/APCI-MS) analysis revealed approximately 58% replacement of hydrogen by deuterium atoms as indicated by the m...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9105-8
更新日期:2006-09-01 00:00:00
abstract::A strain of Serratia sp. showed intracellular electron-transparent inclusion bodies when incubated in the presence of citrate and glycerol 2-phosphate without nitrogen source following pre-growth under carbon-limitation in continuous culture. About 1.3 mmol citrate were consumed per 450 mg biomass, giving a calculated...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9561-9
更新日期:2008-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03026-5
更新日期:2021-02-01 00:00:00