Establishment and optimization of a high-throughput mimic perfusion model in ambr® 15.

abstract：OBJECTIVE:To evaluate the therapeutic potential of human umbilical cord blood mesenchymal stem cells (hUCBMSCs) on promoting erectile function in a rat model of bilateral cavernous nerve (CN) crush injury. RESULTS:Fifty male Sprague-Dawley rats were randomly assigned to sham + PBS group (n = 10), BCNI (bilateral caver...

journal_title：Biotechnology letters

authors： Zhu JQ,Lu HK,Cui ZQ,Wang YC,Li YH,Zhao W,Fu Q,Xu YM,Xu Y,Song LJ

更新日期：2015-07-01 00:00:00

abstract：:The genes involved in the biosynthetic pathway of ectoine (2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid) from Bacillus halodurans were cloned as an operon and expressed in E. coli. Analysis of the deduced ectoine biosynthesis cluster amino acid sequence revealed that the ectoine operon contain 2,389 bp, enc...

journal_title：Biotechnology letters

authors： Anbu Rajan L,Joseph TC,Thampuran N,James R,Ashok Kumar K,Viswanathan C,Bansal KC

更新日期：2008-08-01 00:00:00

abstract：:Fluctuating light intensity had a more significant impact on growth of gametophytes of transgenic Laminaria japonica in a 2500 ml bubble-column bioreactor than constant light intensity. A fluctuating light intensity between 10 and 110 microE m(-2) s(-1), with a photoperiod of 14 h:10 h light:dark, was the best regime ...

journal_title：Biotechnology letters

authors： Gao J,Zhang Y,Zhang W,Wu S,Qin S,Zhang W,Yu X

更新日期：2005-09-01 00:00:00

abstract：:Glycine soja is a species of soybean that survives in adverse environments including high salt and drought conditions. We constructed a cDNA library from G. soja seedlings treated with NaCl and isolated a glutathione S-transferase gene (GsGST: GQ265911) from the library. The cDNA encoding GsGST contains an open readin...

journal_title：Biotechnology letters

authors： Ji W,Zhu Y,Li Y,Yang L,Zhao X,Cai H,Bai X

更新日期：2010-08-01 00:00:00

abstract：OBJECTIVES:This short commentary examines the factors that led to Food and Drug Administration's approval of the first plant-derived biologic. RESULTS:In 2012, the first plant-derived protein pharmaceutical (biologic) was approved for commercial use in humans. The product, a recombinant form of human β-glucocerebrosid...

journal_title：Biotechnology letters

authors： Mor TS

更新日期：2015-11-01 00:00:00

abstract：:The stability (half-life, t(½)) of the large catalase (CAT) isolated from Aspergillus terreus was decreased under acidic conditions (maximum t(½) approximately 8.5 months at pH ≤ 6) versus alkaline conditions (t(½) approximately 15 months at pH 8-12). Acidic conditions induce the dissociation of haem from CAT, as reve...

journal_title：Biotechnology letters

authors： Vatsyayan P,Goswami P

更新日期：2011-02-01 00:00:00

abstract：:Laccase was produced by Coriolopsis rigida using barley bran as substrate in solid-state fermentation (SSF) and also by submerged fermentation (SmF). The best results were obtained in SSF with twice the amount of laccase production. Laccase could be produced from repeated batch cultures of SSF over 30 days. The laccas...

journal_title：Biotechnology letters

authors： Gómez J,Rodríguez Solar D,Pazos M,Sanromán MA

更新日期：2006-07-01 00:00:00

abstract：:Extracts of Salvia species are used in traditional medicine to treat various diseases. The economic importance of this genus has increased in recent years due to evidence that some of its secondary metabolites have valuable pharmaceutical and nutraceutical properties.The bioactivity of sage extracts is mainly due to t...

journal_title：Biotechnology letters

authors： Marchev A,Haas C,Schulz S,Georgiev V,Steingroewer J,Bley T,Pavlov A

更新日期：2014-02-01 00:00:00

abstract：:Tributyl phosphate (TBP) is widely used in nuclear fuel processing and other waste generating chemical industries. Although TBP is bacteriostatic, some microbes are resistant to it and may degrade it. Under dark aerobiosis, purple non-sulfur photosynthetic bacteria degraded up to 0.6 mM TBP, initially present at 2 mM,...

journal_title：Biotechnology letters

authors： Berne C,Allainmat B,Garcia D

更新日期：2005-04-01 00:00:00

abstract：OBJECTIVE:To evaluate the role and the molecular mechanism of miR-30d in non-small cell lung cancer (NSCLC). RESULTS:qRT-PCR was used to detect miR-30d expression in NSCLC tissues and cell lines. miR-30d was frequently down-regulated in NSCLC and its expression was associated with clinicopathological features of NSCLC...

journal_title：Biotechnology letters

authors： Wu Y,Zhang J,Hou S,Cheng Z,Yuan M

更新日期：2017-12-01 00:00:00

abstract：:Using the optimal concentrations of octanoic acid (0.75 mM) and ethyl mercaptan (2 mM), as the most effective sulfur donor, Pseudomonas reptilivora produced 74 microg lipoic acid per dry cell weight at pH 7.5 and 30 degrees C in a fermenter over 9 h. The dry cell weight was 13.9 g l(-1). ...

journal_title：Biotechnology letters

authors： Ji JH,Yu IS,Kim HJ,Oh DK

更新日期：2008-10-01 00:00:00

abstract：:Serratia marcescens SS-1 and its SpnR-defective isogenic mutant, SMdeltaR, produced an extracellular surfactant able to decrease surface tension of water from 72 to 37 dyne cm(-1) (SMdeltaR strain) and to 45 dyne cm(-1) (SS-1 strain). The biosurfactant also emulsified kerosene and diesel with a maximum emulsion index ...

journal_title：Biotechnology letters

authors： Wei YH,Lai HC,Chen SY,Yeh MS,Chang JS

更新日期：2004-05-01 00:00:00

abstract：:The gene encoding a novel short-chain alcohol dehydrogenase in the thermophilic bacterium, Carboxydothermus hydrogenoformans, was identified and overexpressed in Escherichia coli. The enzyme was thermally stable and displayed the highest activity at 70 °C and pH 6.0. It preferred NAD(H) over NADP(H) as a cofactor and ...

journal_title：Biotechnology letters

authors： Zhou S,Zhang SC,Lai DY,Zhang SL,Chen ZM

更新日期：2013-03-01 00:00:00

abstract：:A mutant ofS. chrysomallus with a D-glucose isomerase inducible by D-glucose or its catabolites was characterized. In contrast to the wild-type strain it showed a decreased catabolite repression by D-glucose of D-xylose consumption and a constitutive pentose phosphate pathway as well. A hypothesis concerning altered i...

journal_title：Biotechnology letters

authors： Hänel F,Roth M,Neigenfind M,Gräfe U,Bormann EJ

更新日期：1987-12-01 00:00:00

abstract：:Coupling lactate fermentation with cell growth was investigated in shake-flask and bioreactor cultivation systems by increasing aeration to improve lactate productivity in Escherichia coli CICIM B0013-070 (ackA pta pps pflB dld poxB adhE frdA). In shake-flasks, cells reached 1 g dry wt/l then, cultivated at 100 rpm an...

journal_title：Biotechnology letters

authors： Zhou L,Tian KM,Niu DD,Shen W,Shi GY,Singh S,Wang ZX

更新日期：2012-06-01 00:00:00

abstract：:Brevibacterium epidermis DSM 20659 is a halotolerant Gram-positive bacterium which can synthesize the osmolyte, ectoine, but prefers to take it up from its environment. The present study revealed that B. epidermis is equipped with at least one transport system for ectoine, with a maximal transport velocity of 15.7 +/-...

journal_title：Biotechnology letters

authors： Onraedt A,De Mey M,Walcarius B,Soetaert W,Vandamme EJ

更新日期：2006-11-01 00:00:00

abstract：:Some recombinant proteins expressed by baculovirus expression vector systems (BEVS) aggregate because the BEVS can produce large amounts of protein late during infection, when post-translational modification and protein quality control mechanisms are inactive. For expression during earlier stages than that driven by t...

journal_title：Biotechnology letters

authors： Ishiyama S,Ikeda M

更新日期：2010-11-01 00:00:00

abstract：:Peroxisome proliferator-activated receptors (PPARs) alpha and gamma are key regulators of lipid homeostasis and insulin resistance. In this study we show that a novel compound, 3-{4-[2-(5-methyl-2-phenyl-oxazol-4-yl)-ethoxy]-phenyl}- 2-[2-(2-nitro-phenoxy)-acetyl amino]-propionic acid (O325H), is an agonist with dual ...

journal_title：Biotechnology letters

authors： Xu C,Wang LL,Liu HY,Ruan CM,Zhou XB,Cao YL,Li S

更新日期：2006-06-01 00:00:00

abstract：:Laccases play an important role in the biological break down of lignin and have great potential in the deconstruction of lignocellulosic feedstocks. We examined 16 laccases, both commercially prepared and crude extracts, for their ability to oxidize veratryl alcohol in the presence of various solvents and mediators. S...

journal_title：Biotechnology letters

authors： Larson TM,Anderson AM,Rich JO

更新日期：2013-02-01 00:00:00

abstract：:An aqueous-organic biphasic system was established and used with whole cells of Oenococcus oeni to reduce 2-octanone to (R)-2-octanol. The conversion reached 99% when the Tris/borate buffer was increased from 50 mM to 300 mM in the aqueous phase. In addition, the conversion increased as the log P value of the organic ...

journal_title：Biotechnology letters

authors： Hu J,Xu Y

更新日期：2006-07-01 00:00:00

abstract：:16,17-Epoxysterol plays an important role in pharmaceutical steroid synthesis. To investigate the potential application of cytochrome P450 for epoxysterol synthesis, an approach to the epoxidation of 16,17-epoxysterol, based on directed evolution of cytochrome P450 BM-3, was developed. This comprised random gene mutag...

journal_title：Biotechnology letters

authors： Jiang D,Tu R,Bai P,Wang Q

更新日期：2013-10-01 00:00:00

abstract：:Electroporation of Litopenaeus schmitti embryos was used to transfer the pE300tiGH15 plasmid that contains the tilapia growth hormone gene (tiGH) complexed with a nuclear localization signal peptide into the zygotes. The gene construct was detected in 35 (36%) of the 98 larvae screened by PCR and Southern blot analyse...

journal_title：Biotechnology letters

authors： Arenal A,Pimentel R,Pimentel E,Martín L,Santiesteban D,Franco R,Aleström P

更新日期：2008-05-01 00:00:00

abstract：OBJECTIVES:To develop a colorimetric assay for ATP based on the blue-pigment synthesising non-ribosomal peptide synthetase (NRPS) BpsA, and to demonstrate its utility in defining the substrate specificity of other NRPS enzymes. RESULTS:BpsA is able to convert two molecules of L-glutamine into the readily-detected blue...

journal_title：Biotechnology letters

authors： Brown AS,Calcott MJ,Collins VM,Owen JG,Ackerley DF

更新日期：2020-12-01 00:00:00

abstract：:Ma-pyrG was cloned from Monascus aurantiacus AS3.4384 using degenerate PCR with primers designed with an algorithm called CODEHOP, and its complete sequence was obtained by a PCR-based strategy for screening a Monascus fosmid library. Ma-pyrG encodes orotidine-5'-phosphate decarboxylase (OMPdecase), a 283-aminoacid pr...

journal_title：Biotechnology letters

authors： Wang BH,Xu Y,Li YP

更新日期：2010-11-01 00:00:00

abstract：:Although resistance of microorganisms to Hg(II) salts has been widely investigated and resistant strains have been reported from many eubacterial genera, there are few reports of mercuric ion resistance in extremophilic microorganisms. Moderately thermophilic mercury resistant bacteria were selected by growth at 62 de...

journal_title：Biotechnology letters

authors： Glendinning KJ,Macaskie LE,Brown NL

更新日期：2005-11-01 00:00:00

abstract：:A coupled in silico thermodynamic and probabilistic metabolic control analysis methodology was verified by applying it to the glycerol biosynthetic pathway in Saccharomyces cerevisiae. The methodology allows predictions even when detailed knowledge of the enzyme kinetics is lacking. In a metabolic steady state, we fou...

journal_title：Biotechnology letters

authors： Birkenmeier M,Mack M,Röder T

更新日期：2015-02-01 00:00:00

abstract：:Amberlite XAD 2 resin enhanced the asymmetric reduction of ethyl 4-chloroacetoacetate (ECA) to S-4-chloro-3-hydroxybutyric acid ethyl ester as catalyzed by Saccharomyces cerevisiae. The absorbed ECA was released slowly to the solution during the reaction so that the substrate inhibition and the spontaneous chemical hy...

journal_title：Biotechnology letters

authors： Houng JY,Liau JS

更新日期：2003-01-01 00:00:00

abstract：:The genetics governing the morphological switch from round or ovoid cells to filamentous growth in Saccharomyces cerevisiae has received significant interest in relation to sensing and signaling pathways as well as the control of cell processes including budding, elongation and adhesion. Little is known about the envi...

journal_title：Biotechnology letters

authors： Ceccato-Antonini SR

更新日期：2008-07-01 00:00:00

abstract：:Nitrogenous derivatives of the two orange pigments from Monascus sp. with anti-melanogenic activities were prepared using fermentation and chemical synthesis. The pigments were produced in a 5 l jar fermentor. A total of 33 derivatives were synthesized via incorporation of L-amino acids and amines into the pigments. T...

journal_title：Biotechnology letters

authors： Jo D,Choe D,Nam K,Shin CS

更新日期：2014-08-01 00:00:00

abstract：OBJECTIVE:The growth characteristics and intracellular hemin concentrations of Lactococcus lactis grown under different culture pH and aeration conditions were examined to investigate the effect of culture pH on the respiration efficiency of L. lactis NZ9000 (pZN8148). RESULTS:Cell biomass and biomass yield of L. lact...

journal_title：Biotechnology letters

authors： Shi W,Li Y,Gao X,Fu R

更新日期：2016-03-01 00:00:00