Abstract:
OBJECTIVE:The growth characteristics and intracellular hemin concentrations of Lactococcus lactis grown under different culture pH and aeration conditions were examined to investigate the effect of culture pH on the respiration efficiency of L. lactis NZ9000 (pZN8148). RESULTS:Cell biomass and biomass yield of L. lactis grown with 4 μg hemin/ml and O2 were higher than those without aeration when the culture pH was controlled at 5-6.5. The culture pH affected the respiratory efficiency in the following order of pH: 5 > 5.5 > 6 > 6.5; the lag phase increased as the culture pH decreased. Hemin accumulation was sensitive to culture pH. Among the four pH conditions, pH 5.5 was optimal for hemin accumulation in the cells. The highest intracellular hemin level in L. lactis resting cells incubated at different pH saline levels (5-6.5) was at pH 5.5. CONCLUSION:The respiration efficiency of L. lactis under respiration-permissive conditions increases markedly as the culture pH decreases. These results may help develop high cell-density L. lactis cultures. Thus, this microorganism may be used for industrial applications.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Shi W,Li Y,Gao X,Fu Rdoi
10.1007/s10529-015-1999-6subject
Has Abstractpub_date
2016-03-01 00:00:00pages
495-501issue
3eissn
0141-5492issn
1573-6776pii
10.1007/s10529-015-1999-6journal_volume
38pub_type
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