Abstract:
OBJECTIVES:To find a novel host for the production of 4-vinylphenol (4VPh) by screening Streptomyces species. RESULTS:The conversion of p-coumaric acid (pHCA) to 4VPh in Streptomyces mobaraense was evaluated using a medium containing pHCA. S. mobaraense readily assimilated pHCA after 24 h of cultivation to produce 4VPh. A phenolic acid decarboxylase, derived from S. mobaraense (SmPAD), was purified following heterologous expression in Escherichia coli. SmPAD was evaluated under various conditions, and the enzyme's kcat/Km value was 0.54 mM (-1) s(-1). Using intergenetic conjugation, a gene from Rhodobacter sphaeroides encoding a tyrosine ammonia lyase, which catalyzes the conversion of L-tyrosine to p-coumaric acid, was introduced into S. mobaraense. The resulting S. mobaraense transformant produced 273 mg 4VPh l(-1) from 10 g glucose l(-1). CONCLUSION:A novel strain suitable for the production of 4VPh and potentially other aromatic compounds was isolated.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Fujiwara R,Noda S,Kawai Y,Tanaka T,Kondo Adoi
10.1007/s10529-016-2126-zsubject
Has Abstractpub_date
2016-09-01 00:00:00pages
1543-9issue
9eissn
0141-5492issn
1573-6776pii
10.1007/s10529-016-2126-zjournal_volume
38pub_type
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