Abstract:
OBJECTIVE:To identify and characterize a novel bacterial pyranose 2-oxidase (P2Ox) and investigate its potential use in lignin degradation applications. RESULTS:A new bacterial P2Ox (PaP2Ox) enzyme was identified in the lignocellulolytic bacterium Pantoea ananatis Sd-1. The PaP2Ox open reading frame was cloned, and the encoded protein was heterologously expressed in an Escherichia coli expression system. Unlike another reported bacterial P2Ox enzyme, the purified PaP2Ox exhibits a homotetrameric spatial conformation that is similar to fungal P2Oxs, with each subunit having a molecular mass of 65 kDa. The recombinant PaP2Ox exhibits maximum activity at 50 °C and pH 6.5 with D-glucose as its preferred substrate. In addition, this enzyme was shown to work in combination with bacterial laccase in lignin degradation. CONCLUSIONS:The bacterial enzyme PaP2Ox has potential use in ligninolytic systems and shows promising value in industrial biotechnological applications.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Zhang K,Huang M,Ma J,Liu Z,Zeng J,Liu X,Xu T,Wang X,Liu Y,Bu Z,Zhu Ydoi
10.1007/s10529-018-2538-zsubject
Has Abstractpub_date
2018-05-01 00:00:00pages
871-880issue
5eissn
0141-5492issn
1573-6776pii
10.1007/s10529-018-2538-zjournal_volume
40pub_type
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