Activation of Pyrococcus furiosus alkaline phosphatase by divalent metal ions.

abstract：:Alginate oligosaccharide (AOS) was reported to possess antioxidant and free radical scavenging activities, but the specific effects and mechanisms remain unclear. We investigated the effects of AOS on H2O2-induced oxidative stress and apoptosis in human umbilical vein endothelial cells (HUVECs) and the associated mech...

journal_title：Biotechnology letters

authors： Zhao J,Han Y,Wang Z,Zhang R,Wang G,Mao Y

更新日期：2020-12-01 00:00:00

abstract：:The ion-exchange chromatography behavior of recombinant glucose dehydrogenase harboring pyrroloquinoline quinone (PQQGDH) was modified to greatly simplify its purification. The surface charge of PQQGDH was engineered by either fusing a three-arginine tail to the C-terminus of PQQGDH (PQQGDH+Arg3) or by substituting th...

journal_title：Biotechnology letters

authors： Koh H,Igarashi S,Sode K

更新日期：2003-10-01 00:00:00

abstract：:beta-Galactosidase isolated from Aspergillus oryzae was immobilized in lens-shaped polyvinylalcohol capsules (with activity 25 U g(-1)) giving 32% of its original activity. Immobilization did not change the pH optimum (4.5) of lactose hydrolysis. The relative enzyme activity during product inhibition testing was, in a...

journal_title：Biotechnology letters

authors： Grosová Z,Rosenberg M,Rebros M,Sipocz M,Sedlácková B

更新日期：2008-04-01 00:00:00

abstract：:Nitrite at 2 mM did not affect the growth of Botryococcus braunii and served as the sole nitrogen source giving a minimum biomass doubling time of 4.2 d, which was equal to that of the culture using 4 mM nitrate as nitrogen source. With nitrite at 4 mM, after a lag phase of about 10 d, the alga grew quickly, reaching ...

journal_title：Biotechnology letters

authors： Yang S,Wang J,Cong W,Cai Z,Ouyang F

更新日期：2004-02-01 00:00:00

abstract：:Glycolipid biosurfactants produced by bacteria and yeasts provide significant opportunities to replace chemical surfactants with sustainable biologically produced alternatives in bulk commercial products such as laundry detergents and surface cleaners. Sophorolipids are already available in sufficient yield to make th...

journal_title：Biotechnology letters

authors： Marchant R,Banat IM

更新日期：2012-09-01 00:00:00

abstract：:A sigma 70-like gene, sigA, has been identified from Corynebacterium ammoniagenes. The sigA gene encodes a polypeptide of 467 amino acids with a calculated molecular mass of 52036 Da. The deduced amino acid sequence preserves the common motifs of the primary sigma factors and shows very high similarity to those of Sig...

journal_title：Biotechnology letters

authors： Lee BR,Kim JH

更新日期：2004-04-01 00:00:00

abstract：:In Klebsiella pneumoniae, aldehyde dehydrogenases (ALDH) convert 3-hydroxypropionaldehyde (3-HPA) into 3-hydroxypropionic acid (3-HP). Although ALDHs can increase the production of 3-HP in K. pneumoniae, the substrate specificity of ALDH homologues from other microorganisms toward 3-HPA is less documented. Here we rep...

journal_title：Biotechnology letters

authors： Su M,Li Y,Ge X,Tian P

更新日期：2015-03-01 00:00:00

abstract：:Fed-batch cultivation of Azotobacter vinelandii 21was optimized for biosurfactant production. Optimization of feed-rate time profile and concentration of nutrient medium components in feeding solution is based on a hybrid mathematical model consisting of mass-balance equations for biomass, biosurfactant, volume of cul...

journal_title：Biotechnology letters

authors： Levisauskas D,Galvanauskas V,Zunda G,Grigiskis S

更新日期：2004-07-01 00:00:00

abstract：OBJECTIVES:To exploit cold-inducible biochemical processes beneficial for foreign mRNA transcription, translation and storage, as well as protein product stability, during Agrobacterium-mediated transient expression. RESULTS:The efficiency of three different 5'-regulatory sequences to achieve transient expression of t...

journal_title：Biotechnology letters

authors： Gerasymenko IM,Sheludko YV

更新日期：2017-07-01 00:00:00

abstract：OBJECTIVES:To optimize the production of active inclusion bodies (IBs) containing human D-amino acid oxidase (hDAAO) in Escherichia coli. RESULTS:The optimized initial codon region combined with the coexpressed rare tRNAs, fusion of each of the N-terminal partners including cellulose-binding module, thioredoxin, gluta...

journal_title：Biotechnology letters

authors： Wang W,Sun J,Xiao W,Jiang L,Wang R,Fan J

更新日期：2017-11-01 00:00:00

abstract：:The Rho1 protein is a GTPase that participates in cell wall biogenesis. We analyzed the transcript levels of laccase genes (lccl, lcc2, lcc3, lcc4, lcc5, and lcc9), and a xylanase gene (xyl3) in Fusarium oxysporum f. sp. lycopersici strain 4287 (wild type) and two mutant strains; rhol::hyg that lacks a functional Rho1...

journal_title：Biotechnology letters

authors： Reyes-Medina MA,Macías-Sánchez KL

更新日期：2015-03-01 00:00:00

abstract：:For most cultivated mammalian cells, glutamine is an essential medium component. However, glutamine consumption results in the production of ammonia, a cytotoxic byproduct. Here we investigated the effect of glutamine reduction on recombinant protein production and ammonia accumulation in transiently transfected CHO a...

journal_title：Biotechnology letters

authors： Rajendra Y,Kiseljak D,Baldi L,Hacker DL,Wurm FM

更新日期：2012-04-01 00:00:00

abstract：:Classical swine fever (CSF) is one of the most important viral diseases of swine worldwide. Although live or attenuated virus vaccines have been used to control CSFV, it is difficult to distinguish vaccinated pigs from infected pigs; this leads to restrictions on import and export. Subunit vaccines based on the CSFV E...

journal_title：Biotechnology letters

authors： Park Y,Lee S,Kang H,Park M,Min K,Kim NH,Gu S,Kim JK,An DJ,Choe S,Sohn EJ

更新日期：2020-07-01 00:00:00

abstract：OBJECTIVES:The promoter of HpMET3, encoding an ATP sulfurylase, was evaluated for its potential as a repressible promoter to downregulate the expression of target genes in the thermotolerant, methylotrophic yeast Hansenula polymorpha. RESULTS:The expression of lacZ under the control of the 0.6 kb HpMET3 promoter was e...

journal_title：Biotechnology letters

authors： Yoo SJ,Chung SY,Lee DJ,Kim H,Cheon SA,Kang HA

更新日期：2015-11-01 00:00:00

abstract：:Chloroperoxidase from Caldariomyces fumago catalyses the oxidation of 1,2-dihydronaphthalene to (1R,2R)-(+)-dihydroxytetrahydronaphthalene in homogenous citrate buffer/ionic liquid mixtures, using t-butyl hydroperoxide as O2 donor. It tolerates up to 30 (v/v) 1,3-dimethylimidazolium methylsulfate or 1-butyl-3-methylim...

journal_title：Biotechnology letters

authors： Sanfilippo C,D'Antona N,Nicolosi G

更新日期：2004-12-01 00:00:00

abstract：:Washed cells (62 mg) of Kitasatospora sp. GF12 in 4 ml buffer (pH 7) catalyzed the regioselective hydroxylation of 60 mM 1,3-adamantanediol [1,3-ad(OH)(2)] to 30.9 mM 1,3,5-adamantanetriol [1,3,5-ad(OH)(3)] over 120 h at 24 °C. Glycerol at 400 mM was added to the reaction mixture to recycle the intracellular NADH/NADP...

journal_title：Biotechnology letters

authors： Mitsukura K,Yamanaka N,Yoshida T,Nagasawa T

更新日期：2012-09-01 00:00:00

abstract：:A gene, rBmK Cta, encoding a chlorotoxin-like peptide from the scorpion, Buthus martensii Karsch, was synthesized according to the sequence optimized for codon usage in Escherichia coli and was expressed in E. coli BL21 (DE3) using a pExSecI expression system in which the IgG-binding domain-ZZ of protein A is fused to...

journal_title：Biotechnology letters

authors： Fu YJ,Yin LT,Wang W,Chai BF,Liang AH

更新日期：2005-10-01 00:00:00

abstract：OBJECTIVES:The purpose of this study was to develop a facile and efficient method to enhance the stability and activity of lactoperoxidase (LPO) by using its immobilization on graphene oxide nanosheets (GO-NS). METHODS:Following the LPO purification from bovine whey, it was immobilized onto functionalized GO-NS using ...

journal_title：Biotechnology letters

authors： Shariat SZAS,Borzouee F,Mofid MR,Varshosaz J

更新日期：2018-10-01 00:00:00

abstract：:A novel glucose-sensing molecule was created based on galactose/glucose-binding protein (GGBP). GGBP mutants at Asp14, a residue interacting with the 4th hydroxyl group of the sugar molecule, were constructed by mutagenesis to improve the ligand specificity of GGBP. The autofluorescence-based analysis of the binding a...

journal_title：Biotechnology letters

authors： Sakaguchi-Mikami A,Taneoka A,Yamoto R,Ferri S,Sode K

更新日期：2008-08-01 00:00:00

abstract：:Glucosylceramide and galactosylceramide were detected in three Aspergillus species: Aspergillus oryzae, Aspergillus sojae and Aspergillus. awamori, using borate-coated TLC. The cerebrosides from A. oryzae were further purified by ion exchange and iatrobeads column chromatographies with or without borate, and determine...

journal_title：Biotechnology letters

authors： Tani Y,Amaishi Y,Funatsu T,Ito M,Itonori S,Hata Y,Ashida H,Yamamoto K

更新日期：2014-12-01 00:00:00

abstract：OBJECTIVES:Sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum is one of the major fungal diseases of canola. To develop resistance against this fungal disease, the chit42 from Trichoderma atroviride with chitin-binding domain and polygalacturonase-inhibiting protein 2 (PG1P2) of Phaseolus vulgaris were co-ex...

journal_title：Biotechnology letters

authors： Ziaei M,Motallebi M,Zamani MR,Panjeh NZ

更新日期：2016-06-01 00:00:00

abstract：OBJECTIVES:The aim of the study is to generate a spherical three-dimensional (3D) aggregate of hepatocyte-like cells (HLCs) differentiated from human embryonic stem cells and to investigate the effect of the 3D environment on hepatic maturation and drug metabolism. RESULTS:Quantitative real-time PCR analysis indicated...

journal_title：Biotechnology letters

authors： Choi YJ,Kim H,Kim JW,Yoon S,Park HJ

更新日期：2018-05-01 00:00:00

abstract：:Polyhydroxyalkanoate (PHA) beads, recombinantly produced in Escherichia coli, were functionalized to display lipase B from Candida antarctica as translational protein fusion. The respective beads were characterized in respect to protein content, functionality, long term storage capacity and re-usability. The direct fu...

journal_title：Biotechnology letters

authors： Jahns AC,Rehm BH

更新日期：2015-04-01 00:00:00

abstract：OBJECTIVE:A bio-based process is appealing for purification of L-lactic acid, the major enantiomer of polylactic acid syrup, generated by thermochemical processes at the end of life of PLA-based plastics, from its chiral impurity, D-lactic acid, before reuse. RESULTS:Polylactic acid (PLA), a renewable alternative to p...

journal_title：Biotechnology letters

authors： Chauliac D,Pullammanappallil PC,Ingram LO,Shanmugam KT

更新日期：2015-12-01 00:00:00

abstract：:Fusion expression is a common practice for recombinant protein production. Some fusion tags confer solubility on the target protein whereas others provide affinity handles that facilitate purification. However, the tag usually needs to be removed from the final product, which involves using expensive proteases or haza...

journal_title：Biotechnology letters

authors： Li Y

更新日期：2011-05-01 00:00:00

abstract：:Recombinant coagulation factor VII (FVII) is used as a potential therapeutic intervention in hemophilia patients who produce antibodies against the coagulation factors. Mammalian cell lines provide low levels of expression, however, the Spodoptera frugiperda Sf9 cell line and baculovirus expression system are powerful...

journal_title：Biotechnology letters

authors： Masroori N,Halabian R,Mohammadipour M,Roushandeh AM,Rouhbakhsh M,Najafabadi AJ,Fathabad ME,Salimi M,Shokrgozar MA,Roudkenar MH

更新日期：2010-06-01 00:00:00

abstract：:An automated sequential injection analysis (SIA) system using stop-flow technique was developed to determine methanol concentration by means of the enzymatic reactions of alcohol oxidase and peroxidase. Its application as an on-line device for monitoring Pichia pastoris fermentations producing an heterologous protein ...

journal_title：Biotechnology letters

authors： Surribas A,Cos O,Montesinos JL,Valero F

更新日期：2003-11-01 00:00:00

abstract：:The maltose-binding protein (MBP), which possesses a large number of exposed hydrophobic zones, can be used as a link for the immobilization of growth factors. The amount of immobilized MBP-vascular endothelial growth factors (VEGFs) for polystyrene surface was increased with respect to increasing protein, showing 101...

journal_title：Biotechnology letters

authors： Han M,Park IS,Kim SH,Kim BS,Kim SH

更新日期：2009-11-01 00:00:00

abstract：OBJECTIVE:The dye-decolorizing peroxidase from Bacillus amyloliquefaciens, BaDyP, was identified to be an efficient catalyst for the degradation of phenolic β-ether lignin model dimer guaiacylglycerol-β-guaiacyl ether (GGE) and dyes. RESULTS:Efeb gene encoding BaDyP from B. amyloliquefaciens MN-13 consisted of 1257 bp...

journal_title：Biotechnology letters

authors： Yang J,Gao T,Zhang Y,Wang S,Li H,Li S,Wang S

更新日期：2019-09-01 00:00:00

abstract：:A plasmid, pBGFP, carrying green fluorescent protein (gfp) and benomyl-resistance genes was constructed and transformed into Metarhizium anisopliae. The transformants grew normally and GFP fluorescence was detected. No change was found in virulence for the transformants. Fluorescence was detected in hyphae from the ha...

journal_title：Biotechnology letters

authors： Cao Y,Peng G,He Z,Wang Z,Yin Y,Xia Y

更新日期：2007-06-01 00:00:00