Abstract：
:Alginate oligosaccharide (AOS) was reported to possess antioxidant and free radical scavenging activities, but the specific effects and mechanisms remain unclear. We investigated the effects of AOS on H2O2-induced oxidative stress and apoptosis in human umbilical vein endothelial cells (HUVECs) and the associated mechanisms. HUVECs were treated with 100-800 µM hydrogen peroxide (H2O2) for various periods (12, 24, and 36 h) to establish an in vitro oxidative stress and apoptosis HUVEC model. AOS protects HUVEC cells against oxidative stress-induced apoptosis by decreasing the expression levels of caspase 3 and Bax, and increasing Bcl-2 expression. Microarray assay, real-time PCR and western blot results revealed that AOS was able to effectively suppress H2O2-induced apoptosis via regulated integrin-α/FAK/PI3K pathway by influencing the expression of integrin-α, FAK, PI3K, PTEN, P21, and CDK2. In conclusion, our study suggests that AOS can protect endothelial cells against oxidative stress injury caused by H2O2, providing novel alternative strategies to prevent atherosclerosis in the future.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Zhao J,Han Y,Wang Z,Zhang R,Wang G,Mao Ydoi
10.1007/s10529-020-03010-zsubject
Has Abstractpub_date
2020-12-01 00:00:00pages
2749-2758issue
12eissn
0141-5492issn
1573-6776pii
10.1007/s10529-020-03010-zjournal_volume
42pub_type
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doi：10.1007/s10529-018-2566-8
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pub_type： 杂志文章
doi：10.1007/s10529-008-9757-7
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更新日期：2008-09-01 00:00:00
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更新日期：2012-10-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1007/s10529-009-9920-9
更新日期：2009-05-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
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更新日期：2006-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2011-03-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-011-0544-5
更新日期：2011-06-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-006-9105-8
更新日期：2006-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2012-07-01 00:00:00
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pub_type： 杂志文章
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更新日期：2020-07-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-007-9616-y
更新日期：2008-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s10529-004-6934-1
更新日期：2005-01-01 00:00:00
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doi：10.1023/b:bile.0000004389.53388.d0
更新日期：2003-12-01 00:00:00
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更新日期：2010-07-01 00:00:00
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journal_title：Biotechnology letters
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更新日期：2005-04-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-011-0720-7
更新日期：2011-12-01 00:00:00
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更新日期：2018-01-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章,评审
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更新日期：2011-10-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-018-2538-z
更新日期：2018-05-01 00:00:00
abstract：:Hydrophobin-fused domain III of dengue envelope proteins serotypes 1 and 2 were expressed in Rachiplusia nu larvae and purified by aqueous two-phase system. This biotechnological approach of hydrophobin-fused proteins, which allowed obtaining 97.7 µg/larva of fusion protein DomIII serotype 1 and 61.4 µg/larva of fusio...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-019-02767-2
更新日期：2020-03-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-015-2000-4
更新日期：2016-03-01 00:00:00
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journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-016-2051-1
更新日期：2016-05-01 00:00:00
abstract：OBJECTIVES:To investigate the effect of full-length fragment of DNA topoisomerase I gene (TOP1) matrix attachment regions (MARs) originating from the human genome on transgene expression in Chinese hamster ovary (CHO) cells and explore the underlying mechanisms. RESULTS:Results showed that TOP1 MAR cannot only enhance...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-019-02673-7
更新日期：2019-07-01 00:00:00
abstract：:The current study aimed to assess the inhibitory effect of a DNA aptamer (Apt17) which targeted Salmonella invasion proteinA (SipA). The effect of Apt17, on biofilm formation by two Salmonella enteritidis strains, was tested either separately or in combination with ampicillin at different Sub MIC concentrations. Maxim...
journal_title：Biotechnology letters
pub_type： 杂志文章
doi：10.1007/s10529-020-02920-2
更新日期：2020-10-01 00:00:00
