Abstract:
:Rhizopus arrhizus, strain DAR 36017, produced L(+)-lactic acid in a simultaneous saccharification and fermentation process using starch waste effluents. Lactic acid at 19.5-44.3 g l(-1) with a yield of 0.85-0.96 g g(-1) was produced in 40 h using 20-60 g starch l(-1). Supplementation of nitrogen source may be unnecessary if potato or corn starch waste effluent was used as a production medium.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Jin B,Huang LP,Lant Pdoi
10.1023/b:bile.0000004389.53388.d0keywords:
subject
Has Abstractpub_date
2003-12-01 00:00:00pages
1983-7issue
23eissn
0141-5492issn
1573-6776journal_volume
25pub_type
杂志文章abstract:OBJECTIVES:To improve heterologous proteins production, we constructed a maltose-inducible expression system in Bacillus subtilis. RESULTS:An expression system based on the promoter for maltose utilization constructed in B. subtilis. Successively, to improve the performance of the P malA -derived system, mutagenesis w...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2357-7
更新日期:2017-08-01 00:00:00
abstract::An on-line device, ORP (oxidation-reduction potential)-stat, was used to control glucose-feeding for enhancing xylitol conversion from D-xylose during an oxygen-limited fermentation by Candida tropicalis. The fermentation was carried out in a 5 l jar fermenter. After glucose in the medium was depleted, a switching to ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:
更新日期:2003-12-01 00:00:00
abstract::A coupled in silico thermodynamic and probabilistic metabolic control analysis methodology was verified by applying it to the glycerol biosynthetic pathway in Saccharomyces cerevisiae. The methodology allows predictions even when detailed knowledge of the enzyme kinetics is lacking. In a metabolic steady state, we fou...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1675-2
更新日期:2015-02-01 00:00:00
abstract::Ethanol, added as a de-emulsifier to separate oil and biocatalyst (or bacterial cells) from a three-phase (oil/biocatalyst/aqueous phase) emulsion, formed in diesel biodesulfurization employing Gordonia nitida, improved oil recovery by centrifugation from about 50% in its absence to almost 100% at 3% (v/v). The biocat...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1021750719337
更新日期:2003-01-01 00:00:00
abstract::Gene therapy is based on the principle of the genetic manipulation of DNA or RNA for treating and preventing human diseases. The clustered regularly interspaced short palindromic repeats/CRISPR associated nuclease9 (CRISPR/Cas9) system, derived from the acquired immune system in bacteria and archaea, has provided a ne...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-018-2555-y
更新日期:2018-06-01 00:00:00
abstract::Recombinant human interleukin-1 receptor antagonist (rHuIL-1ra) was produced in E. coli as an inclusion body. rHuIL-1ra was purified to Over 98% purity by anion exchange chromatography after on-column refolding. The optimized processes produced more than 2 g pure refolded rHuIL-1ra per 1 l culture, corresponding to a ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-8655-5
更新日期:2005-08-01 00:00:00
abstract::In order to treat the kitchen wastes and produce hydrogen, anaerobic fermentation technology was used in this experiment. The results showed that the fermentation type changed from mixed acid fermentation to ethanol fermentation in a continuous stirred tank reactor (CSTR) 22 days after start-up. The maximum efficiency...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0024-3
更新日期:2009-09-01 00:00:00
abstract::Malic enzyme (ME) catalyses the oxidative decarboxylation of L-malate to pyruvate and provides NADPH for intracellular metabolism, such as fatty acid synthesis. Here, the mitochondrial ME (mME) gene from Mortierella alpina was homologously over-expressed. Compared with controls, fungal arachidonic acid (ARA; 20:4 n-6)...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1546-x
更新日期:2014-09-01 00:00:00
abstract::Growth of Ruta graveolens shoots was induced when Bacillus sp. cell lysates were added to the culture medium. Elicitation of coumarin by this lysate was also very effective; the concentrations of isopimpinelin, xanthotoxin and bergapten increased to 610, 2120 and 1460 microg g(-1) dry wt, respectively. It also had a s...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9559-3
更新日期:2008-03-01 00:00:00
abstract::Cotton fabric samples were treated with a formulation of a total crude Trichoderma reesei cellulase in a two-step procedure. In the first step, samples were treated at a low liquor ratio by padding through the enzyme formulation at 21 degrees C and 55 degrees C with a wet pickup of 100% and batched for 12 h. The sampl...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9569-1
更新日期:2008-03-01 00:00:00
abstract:OBJECTIVES:To improve the production of α-ketoglutaric acid (α-KG) from L-glutamate by whole-cell biocatalysis. RESULTS:A novel and highly active L-glutamate oxidase, SmlGOX, from Streptomyces mobaraensis was overexpressed and purified. The recombinant SmlGOX was approx. 64 kDa by SDS-PAGE. SmlGOX had a maximal activi...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2314-5
更新日期:2017-06-01 00:00:00
abstract:OBJECTIVES:The purpose of this study was to develop a facile and efficient method to enhance the stability and activity of lactoperoxidase (LPO) by using its immobilization on graphene oxide nanosheets (GO-NS). METHODS:Following the LPO purification from bovine whey, it was immobilized onto functionalized GO-NS using ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2583-7
更新日期:2018-10-01 00:00:00
abstract::The lipase of Pseudomonas cepacia was immobilized onto electrospun polyacrylonitrile (PAN) fibers and used for the conversion of (S)-glycidol with vinyl n-butyrate to glycidyl n-butyrate in isooctane. The rate of reaction with the adsorbed lipase was 23-fold higher than the initial material. After 10 recyclings, the i...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0279-8
更新日期:2010-08-01 00:00:00
abstract::Two genes, MGFc (40) and MGFc (24), encoding different E peptides of mechano-growth factor (MGF), were obtained by a four-step PCR strategy and subcloned into pRSETC and pGEX-6p-1. Recombinant MGFc(40) protein (4 mg l(-1)) was expressed and purified by affinity chromatog...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0762-x
更新日期:2012-02-01 00:00:00
abstract:OBJECTIVE:This study aimed to investigate the inhibitory effect of berberine hydrochloride (BH) on Candida albicans (C.albicans) ATCC10231 biofilm formation. RESULTS:This paper found a positive correlation between the concentration of BH and its inhibitory effect on the cellular activity of early biofilms because we f...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-02938-6
更新日期:2020-11-01 00:00:00
abstract:OBJECTIVES:To develop a rapid, dual-parameter, plate-based screening process to improve production and secretion rate of glucose oxidase simultaneously in Aspergillus niger. RESULTS:A morphology engineering based on CaCO3 was implemented, where the yield of GOD by A. niger was increased by up to 50%. Analysis of extra...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2442-y
更新日期:2018-01-01 00:00:00
abstract::A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amy...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5794-7
更新日期:2005-06-01 00:00:00
abstract:OBJECTIVES:Developing an effective HIV vaccine that stimulates the humoral and cellular immune responses is still challenging because of the diversity of HIV-1 virus, polymorphism of human HLA and lack of a suitable delivery system. RESULTS:Using bioinformatics tools, we designed a DNA construct encoding multiple epit...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-019-02734-x
更新日期:2019-11-01 00:00:00
abstract::An optimal pooling system, called Accurate and Fast Target Screening, has been developed for high-throughput identifying the rare marker-free transformants. This system can identify targets between 10- and 100-fold more efficiently than analysis of individual samples. By calculating the efficiency for different propor...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9129-0
更新日期:2006-10-01 00:00:00
abstract::Classical swine fever (CSF) is one of the most important viral diseases of swine worldwide. Although live or attenuated virus vaccines have been used to control CSFV, it is difficult to distinguish vaccinated pigs from infected pigs; this leads to restrictions on import and export. Subunit vaccines based on the CSFV E...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-02892-3
更新日期:2020-07-01 00:00:00
abstract::The effects of glycerol and the oxygen transfer rate on the xylitol production rate by a xylitol dehydrogenase gene (XYL2)-disrupted mutant of Candida tropicalis were investigated. The mutant produced xylitol near the almost yield of 100% from D: -xylose using glycerol as a co-substrate for cell growth and NADPH regen...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9068-9
更新日期:2006-08-01 00:00:00
abstract:OBJECTIVES:To acquire a thermostable xylanase, that is suitable for xylooligosaccharide production from pretreated corncobs, the metagenomic method was used to obtain the gene from an uncultured environmental microorganism. RESULTS:A thermostable xylanase-encoding gene (xyn10CD18) was cloned directly from the metageno...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1857-6
更新日期:2015-09-01 00:00:00
abstract::The ability of grape skins to catalyze in vitro conversion of p-coumaric acid to the more potent antioxidant caffeic acid was studied. Addition of different concentrations of p-coumaric to red grape skins (Cabernet Sauvignon) resulted in formation of caffeic acid. This caffeic acid formation (Y) correlated positively ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0103-5
更新日期:2009-12-01 00:00:00
abstract:OBJECTIVE:Silk sericin is a natural polymer with potential utility in biomedical and biotechnological applications. Recombinantly expressed sericin ensures a source of pure protein with no contamination and with multiple properties when expressed as a fusion protein. Hence, the present paper aims to recombinantly expre...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-02911-3
更新日期:2020-09-01 00:00:00
abstract::The biomacromolecule, gelatin, has increasingly been used in biomedicine-beyond its traditional use in food and cosmetics. The appealing advantages of gelatin, such as its cell-adhesive structure, low cost, off-the-shelf availability, high biocompatibility, biodegradability and low immunogenicity, among others, have m...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-015-1907-0
更新日期:2015-11-01 00:00:00
abstract::For most cultivated mammalian cells, glutamine is an essential medium component. However, glutamine consumption results in the production of ammonia, a cytotoxic byproduct. Here we investigated the effect of glutamine reduction on recombinant protein production and ammonia accumulation in transiently transfected CHO a...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0809-z
更新日期:2012-04-01 00:00:00
abstract::Polyhydroxyalkanoate (PHA) beads, recombinantly produced in Escherichia coli, were functionalized to display lipase B from Candida antarctica as translational protein fusion. The respective beads were characterized in respect to protein content, functionality, long term storage capacity and re-usability. The direct fu...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1735-7
更新日期:2015-04-01 00:00:00
abstract::Neural cortical cells, isolated from prenatal rat cerebra, were grown on surface-modified poly(lactic-co-glycolic acid, 65:35) (PLGA) films coated with poly-D: -lysine (PDL) with either laminin (LN), fibronectin (FN) or collagen (CN). Immunocytochemistry showed that the isolated cells were highly immunopositive for bo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-6585-2
更新日期:2005-01-01 00:00:00
abstract::To elucidate the functional significance of heat-shock protein beta 1 (HSPB1) in androgen-mediated myogenesis of bovine cells, we conducted 'loss and gain of function of HSPB1' assays by siRNA inhibition and gene overexpression. siRNA inhibition of HSPB1 expression reduced the expression of desmin (a myogenic marker) ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1489-2
更新日期:2014-06-01 00:00:00
abstract::A gene-specific, metagenomic PCR method has led to the discovery of a novel esterase subfamily consisting of five homologous members. Sequence analysis of this esterase subfamily, named the ArmEst subfamily, revealed a unique conserved pattern with a significant variable interior sequence flanked by two symmetric and ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1293-4
更新日期:2013-11-01 00:00:00