Enhanced neurite outgrowth of rat neural cortical cells on surface-modified films of poly(lactic-co-glycolic acid).

abstract：:The protective effects of polymer additives, including a group of viscosity-enhancing polymer poly-γ-glutamic acid (γPGA; 10, 50, and 500 kDa) and surface-active polymer Pluronic F68, on Chinese hamster ovary cells against damage due to shear stress were investigated in shake-flask cultures. The level of protection wa...

journal_title：Biotechnology letters

authors： Chun BH,Lee YK,Chung N

更新日期：2012-10-01 00:00:00

abstract：:A novel epoxide hydrolase (EHase) from polycyclic aromatic hydrocarbon (PAH)-degrading bacteria was identified and characterized. EHase activity was identified in four strains of PAH-degrading bacteria isolated from commercial gasoline and oil-contaminated sediment based on their growth on styrene oxide and its deriva...

journal_title：Biotechnology letters

authors： Woo JH,Kwon TH,Kim JT,Kim CG,Lee EY

更新日期：2013-04-01 00:00:00

abstract：OBJECTIVE:The purpose of the article is to establish a quick enrichment and detection method using immunomagnetic beads and flow cytometry to analyze circulating tumor cells (CTCs) in the peripheral blood. RESULTS:After incubation with CD326-PE and CD45-APC antibodies, more than 60% MCF7 cells in M-Buffer could be det...

journal_title：Biotechnology letters

authors： Hu L,Chen X,Chen M,Fang J,Nie J,Dai H

更新日期：2021-01-01 00:00:00

abstract：:3,4-Dihydroxybenzoate decarboxylase in Enterobacter cloacae P241 was induced by adding 3,4-dihydroxybenzoic acid, 3-hydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid or 4-acetamidobenzoic acid to the culture medium. After stabilizing the enzyme activity by adding 5 mM dithiothreitol and 20 mM Na(2)S(2)O(3) to a cell-f...

journal_title：Biotechnology letters

authors： Yoshida T,Inami Y,Matsui T,Nagasawa T

更新日期：2010-05-01 00:00:00

abstract：:Dodecanol (1% v/v) and dodecanoic acid (1% w/v) inhibited growth of Yarrowia lipolytica in complex media supplemented with glucose but dodecanedioic acid (1% w/v) was not toxic. Dodecanol-tolerant strains were prepared from the wild type strain H222 as well as the acyl-CoA oxidase deleted (deltaPOX2, POX3, POX5) strai...

journal_title：Biotechnology letters

authors： Smit MS,Mokgoro MM,Setati E,Nicaud JM

更新日期：2004-05-01 00:00:00

abstract：:An acoustic cell settler (ACS) using ultrasound at cells of 3 MHz was used to recycle Saccharomyces cerevisiae in a fermenter. The locations of both the inlet and outlet in the acoustic cell settler, which have a relatively long distance between the transducer and reflector, were optimized. A tilted settler was design...

journal_title：Biotechnology letters

authors： Hwang SH,Koo YM

更新日期：2003-02-01 00:00:00

abstract：:Classical swine fever (CSF) is one of the most important viral diseases of swine worldwide. Although live or attenuated virus vaccines have been used to control CSFV, it is difficult to distinguish vaccinated pigs from infected pigs; this leads to restrictions on import and export. Subunit vaccines based on the CSFV E...

journal_title：Biotechnology letters

authors： Park Y,Lee S,Kang H,Park M,Min K,Kim NH,Gu S,Kim JK,An DJ,Choe S,Sohn EJ

更新日期：2020-07-01 00:00:00

abstract：OBJECTIVES:Establish a complete and efficient method for the preparation of cis-5-hydroxy-L-pipecolic acids (cis-5HPA), including biotransformation and isomers separation and purification. RESULTS:For non-heme Fe(II)/α-KG-dependent dioxygenases, α-ketoglutarate (α-KG) has great influence on the stability of Fe(II) ion...

journal_title：Biotechnology letters

authors： Lu F,Chen J,Ye H,Wu H,Sha F,Huang F,Cao F,Wei P

更新日期：2020-12-01 00:00:00

abstract：:Hydrophobin-fused domain III of dengue envelope proteins serotypes 1 and 2 were expressed in Rachiplusia nu larvae and purified by aqueous two-phase system. This biotechnological approach of hydrophobin-fused proteins, which allowed obtaining 97.7 µg/larva of fusion protein DomIII serotype 1 and 61.4 µg/larva of fusio...

journal_title：Biotechnology letters

authors： Cerezo J,Targovnik AM,Smith ME,González Maglio D,Luppo VC,Morales MA,Miranda MV,Rodríguez Talou J

更新日期：2020-03-01 00:00:00

abstract：OBJECTIVES:To prepare (R)-phenyl-1,2-ethanediol ((R)-PED) with high enantiomeric excess (ee p) and yield from racemic styrene oxide (rac-SO) at high concentration by bi-enzymatic catalysis. RESULTS:The bi-enzymatic catalysis was designed for enantioconvergent hydrolysis of rac-SO by a pair of novel epoxide hydrolases ...

journal_title：Biotechnology letters

authors： Wang R,Hu D,Zong X,Li J,Ding L,Wu M,Li J

更新日期：2017-12-01 00:00:00

abstract：:To develop novel antibiotic peptides useful as therapeutic drugs, a number of analogues were designed to increase the hydrophobic helix region either by Trp-substitution or net positive charge increase by Lys-substitution, from HP(2-9)-ME(1-12). The antibiotic activities of these peptides were evaluated using bacteria...

journal_title：Biotechnology letters

authors： Park Y,Kim HN,Park SN,Jang SH,Choi CH,Lim HT,Hahm KS

更新日期：2004-03-01 00:00:00

abstract：:Fed-batch cultivation of Azotobacter vinelandii 21was optimized for biosurfactant production. Optimization of feed-rate time profile and concentration of nutrient medium components in feeding solution is based on a hybrid mathematical model consisting of mass-balance equations for biomass, biosurfactant, volume of cul...

journal_title：Biotechnology letters

authors： Levisauskas D,Galvanauskas V,Zunda G,Grigiskis S

更新日期：2004-07-01 00:00:00

abstract：:Kluyveromyces marxianus was grown in submerged culture in a complex medium with several potential inducers of lipolytic activity (triacylglycerols, fatty acids). The highest extracellular lipolytic enzyme production (about 80 U ml(-1) in 3 d) was obtained when the medium was supplemented with 2 g urea l(-1) plus 5 g t...

journal_title：Biotechnology letters

authors： Deive FJ,Costas M,Longo MA

更新日期：2003-09-01 00:00:00

abstract：:Mucor circinelloides and Mortierella isabellina hydroxylated dehydroabietic acid (DehA). DehA was converted regio- and stereo-selectively by whole cells of Mr. circinelloides to give 2alpha-hydroxydehydroabietic acid in a 75% molar conversion yield (11 mM from 14.7 mM DehA) after 72 h in the cultivation medium contain...

journal_title：Biotechnology letters

authors： Mitsukura K,Imoto T,Nagaoka H,Yoshida T,Nagasawa T

更新日期：2005-09-01 00:00:00

abstract：:L-Lactic acid was produced from raw cassava starch, by simultaneous enzyme production, starch saccharification and fermentation in a circulating loop bioreactor with Aspergillus awamori and Lactococcus lactis spp. lactis immobilized in loofa sponge. A. awamori was immobilized directly in cylindrical loofa sponge while...

journal_title：Biotechnology letters

authors： Roble ND,Ogbonna JC,Tanaka H

更新日期：2003-07-01 00:00:00

abstract：OBJECTIVES:Interactions of cells with their neighbors and influences by the surrounding extracellular matrix (ECM) is reflected in a cells transcriptome and proteome. In tissues comprised of heterogeneous cell populations or cells depending on ECM signalling cues such as those of the intervertebral disc (IVD), this inf...

journal_title：Biotechnology letters

authors： Li K,Varden L,Henderson A,Lufkin T,Kraus P

更新日期：2021-01-01 00:00:00

abstract：OBJECTIVES:To exploit cold-inducible biochemical processes beneficial for foreign mRNA transcription, translation and storage, as well as protein product stability, during Agrobacterium-mediated transient expression. RESULTS:The efficiency of three different 5'-regulatory sequences to achieve transient expression of t...

journal_title：Biotechnology letters

authors： Gerasymenko IM,Sheludko YV

更新日期：2017-07-01 00:00:00

abstract：:Pleurotus eryngii was transformed using a polyethylene glycol-mediated method. A plasmid, pEPUGH, containing a reporter gene (enhanced green fluorescent protein gene, egfp) and a positive selectable marker gene (hygromycin phosphotransferase gene, hph) was constructed. The fused egfp-hph gene was placed under the cont...

journal_title：Biotechnology letters

authors： Yin Y,Liu Y,Jin H,Wang S,Zhao S,Geng X,Li M,Xu F

更新日期：2012-10-01 00:00:00

abstract：:A novel glucose-sensing molecule was created based on galactose/glucose-binding protein (GGBP). GGBP mutants at Asp14, a residue interacting with the 4th hydroxyl group of the sugar molecule, were constructed by mutagenesis to improve the ligand specificity of GGBP. The autofluorescence-based analysis of the binding a...

journal_title：Biotechnology letters

authors： Sakaguchi-Mikami A,Taneoka A,Yamoto R,Ferri S,Sode K

更新日期：2008-08-01 00:00:00

abstract：:Over-expression of the gene, mshA, coding for mycothiol glycosyl transferase improved the robustness of Corynebacterium glutamicum to various stresses. Intracellular mycothiol (MSH) content was increased by 114 % in WT(pXMJ19-mshA) compared to WT(pXMJ19). Survival rates increased by 44, 39, 90, 77, 131, 87, 52, 47, 57...

journal_title：Biotechnology letters

authors： Liu YB,Chen C,Chaudhry MT,Si MR,Zhang L,Wang Y,Shen XH

更新日期：2014-07-01 00:00:00

abstract：OBJECTIVES:Breast cancer is a popular fatal malignant tumor for women with high of rates incidence and mortality. Development of the new approaches for breast cancer targeted diagnosis and chemotherapy is emergently needed by the current clinical practice, the important first step is finding a breast cancer specificall...

journal_title：Biotechnology letters

authors： Jin H,Gao X,Xiao L,He H,Cheng S,Zhang C,Hou Y,Song F,Su X,Gao Q,Lu Z,Yang R,Song X,Yang J,Duan W,Hou Y

更新日期：2021-01-01 00:00:00

abstract：:Cotton fabric samples were treated with a formulation of a total crude Trichoderma reesei cellulase in a two-step procedure. In the first step, samples were treated at a low liquor ratio by padding through the enzyme formulation at 21 degrees C and 55 degrees C with a wet pickup of 100% and batched for 12 h. The sampl...

journal_title：Biotechnology letters

authors： Schimper CB,Ibanescu C,Keckeis R,Bechtold T

更新日期：2008-03-01 00:00:00

abstract：:Promoters belong to the most important regulatory domains in genomic sequences. A novel cloning method is described to produce various DNA fragments with the average size about 100 bp, which differ by 1 bp, for screening of the osmotic pressure-inducible promoters from the genome of Escherichia coli. An osmotic pressu...

journal_title：Biotechnology letters

authors： Wang Z,Jin L,Wegrzyn G,Wegrzyn A

更新日期：2008-04-01 00:00:00

abstract：:Using the optimal concentrations of octanoic acid (0.75 mM) and ethyl mercaptan (2 mM), as the most effective sulfur donor, Pseudomonas reptilivora produced 74 microg lipoic acid per dry cell weight at pH 7.5 and 30 degrees C in a fermenter over 9 h. The dry cell weight was 13.9 g l(-1). ...

journal_title：Biotechnology letters

authors： Ji JH,Yu IS,Kim HJ,Oh DK

更新日期：2008-10-01 00:00:00

abstract：:Monascus aurantiacus produces high amounts of citrinin which is a mycotoxin with nephrotoxic activity. Six putative citrinin biosynthesis genes have been discovered in M. purpureus and at least 10 genes are responsible for its biosynthesis. However, the sequence of citrinin pathway gene cluster in M. aurantiacus has n...

journal_title：Biotechnology letters

authors： Li YP,Xu Y,Huang ZB

更新日期：2012-01-01 00:00:00

abstract：:The effects of glycerol and the oxygen transfer rate on the xylitol production rate by a xylitol dehydrogenase gene (XYL2)-disrupted mutant of Candida tropicalis were investigated. The mutant produced xylitol near the almost yield of 100% from D: -xylose using glycerol as a co-substrate for cell growth and NADPH regen...

journal_title：Biotechnology letters

authors： Ko BS,Rhee CH,Kim JH

更新日期：2006-08-01 00:00:00

abstract：:Extracts of Salvia species are used in traditional medicine to treat various diseases. The economic importance of this genus has increased in recent years due to evidence that some of its secondary metabolites have valuable pharmaceutical and nutraceutical properties.The bioactivity of sage extracts is mainly due to t...

journal_title：Biotechnology letters

authors： Marchev A,Haas C,Schulz S,Georgiev V,Steingroewer J,Bley T,Pavlov A

更新日期：2014-02-01 00:00:00

abstract：:The effects of a water-miscible ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF(4)]), on both thermodynamics and kinetic mechanism of the horseradish peroxidase (HRP)-catalyzed oxidation of guaiacol (2-methoxyphenol) by H(2)O(2) were investigated. The ionic liquid stabilized the ground state of ...

journal_title：Biotechnology letters

authors： Hong ES,Kwon OY,Ryu K

更新日期：2008-03-01 00:00:00

abstract：:Chlorella protothecoides, a lutein-producing microalga, was grown aerobically in a mineral medium prepared with 70% (v/v) deuterated water. HPLC/atmospheric pressure chemical ionization-mass spectrometry (HPLC/APCI-MS) analysis revealed approximately 58% replacement of hydrogen by deuterium atoms as indicated by the m...

journal_title：Biotechnology letters

authors： Bhosale P,Serban B,Bernstein PS

更新日期：2006-09-01 00:00:00

abstract：:Cell division of the wild type strain Corynebacterium (formerly Brevibacterium) ammoniagenes ATCC 6872 which requires 1 microM Mn2+ for balanced growth was inhibited by addition of 20 mM hydroxyurea (HU) or 10 mM p-methoxyphenol (MP) to a Mn2+-supplemented fermentation medium at an appropriate time. Scanning electron ...

journal_title：Biotechnology letters

authors： Abbouni B,Elhariry HM,Auling G

更新日期：2003-01-01 00:00:00