Enrichment and detection of circulating tumor cells by immunomagnetic beads and flow cytometry.

abstract：:Coupling lactate fermentation with cell growth was investigated in shake-flask and bioreactor cultivation systems by increasing aeration to improve lactate productivity in Escherichia coli CICIM B0013-070 (ackA pta pps pflB dld poxB adhE frdA). In shake-flasks, cells reached 1 g dry wt/l then, cultivated at 100 rpm an...

journal_title：Biotechnology letters

authors： Zhou L,Tian KM,Niu DD,Shen W,Shi GY,Singh S,Wang ZX

更新日期：2012-06-01 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) is a heterodimer containing 66 kDa p66 and 51 kDa p51 subunits. We previously showed that HIV-1 group M (HIV-1 M) RT and HIV-1 group O (HIV-1 O) RT have higher affinities for dTTP and template-primer (T/P) than Moloney murine leukemia virus RT, whi...

journal_title：Biotechnology letters

authors： Nishimura K,Shinomura M,Konishi A,Yasukawa K

更新日期：2013-12-01 00:00:00

abstract：:Nitrite at 2 mM did not affect the growth of Botryococcus braunii and served as the sole nitrogen source giving a minimum biomass doubling time of 4.2 d, which was equal to that of the culture using 4 mM nitrate as nitrogen source. With nitrite at 4 mM, after a lag phase of about 10 d, the alga grew quickly, reaching ...

journal_title：Biotechnology letters

authors： Yang S,Wang J,Cong W,Cai Z,Ouyang F

更新日期：2004-02-01 00:00:00

abstract：:miR-21 is highly expressed in a variety of cancers, suggesting that it might play a role in the process of oncogenesis, as supported by it directly causing pre-B cell lymphomas in transgenic mice. Rm21LG transgenic mice for the conditional co-expression of miR-21 and luciferase (Luc) mediated by Cre/lox P system were ...

journal_title：Biotechnology letters

authors： Du T,Jia J,Lin X,Xie R,Li J,Xiao D,Xu K

更新日期：2014-01-01 00:00:00

abstract：:1,3-Propanediol production by Klebsiella pneumoniae was studied in batch cultures under N2 flow and four airflow systems. Different byproducts were formed under different aeration conditions. An anaerobic/aerobic combined fed-batch culture was developed giving 70 g 1,3-propanediol l(-1) and 16 g 2,3-butanediol l(-1) w...

journal_title：Biotechnology letters

authors： Cheng KK,Liu DH,Sun Y,Liu WB

更新日期：2004-06-01 00:00:00

abstract：OBJECTIVE:To evaluate the quantity of Spirulina cultured in seawater, salt-tolerant strains were screened out and their growth and antioxidant accumulation were studied in different salt concentrations RESULTS:Salt tolerance of five Spirulina strains were investigated with modified Zarrouk medium (with 200-800 mM NaCl...

journal_title：Biotechnology letters

authors： Liu C,Li LJ,Wu CY,Guo KN,Li JH

更新日期：2016-07-01 00:00:00

abstract：OBJECTIVES:To investigated the potential of a novel dendrosomal nanoformulation of curcumin (DNC) in blocking radiation-induced changes in irradiated human umbilical vein endothelial cells (HUVECs), and their adhesion to human THP-1 monocytoid cells. RESULTS:Co60 gamma rays reduced viability, raised the expression of ...

journal_title：Biotechnology letters

authors： Soltani B,Bodaghabadi N,Mahpour G,Ghaemi N,Sadeghizadeh M

更新日期：2016-12-01 00:00:00

abstract：:The substrate specificity of alpha-chymotrypsin and other serine proteases, trypsin, elastase, proteinase K and subtilisin, towards hydrolysis of various polyesters was examined using poly(L-lactide) (PLA), poly(beta-hydroxybutyrate) (PHB), poly(ethylene succinate) (PES), poly(ethylene adipate) (PEA), poly(butylene su...

journal_title：Biotechnology letters

authors： Lim HA,Raku T,Tokiwa Y

更新日期：2005-04-01 00:00:00

abstract：:The proteome of rumen epithelial tissue was analysed by SDS-PAGE coupled with LC-MS/MS. 813 non-redundant proteins were identified of which 7.4 % featured membrane-spanning domains and 15.4 % harboured a signal peptide. According to the gene ontology annotation, the most abundant proteins exhibited binding activities ...

journal_title：Biotechnology letters

authors： Yang Y,Wang J,Yuan T,Bu D,Yang J,Sun P

更新日期：2013-11-01 00:00:00

abstract：:To understand how molecular damage under harsh environmental conditions can be controlled, we investigated the properties of ATPase activity of the chaperonin molecular machinery from the hyperthermophilic archaeon Pyrococcus furiosus (PfCPN). PfCPN ATPase activity depended on K(+) and Mg(2+) and its optimal pH was 7....

journal_title：Biotechnology letters

authors： Chen HY,Tan XL,Lu J,Zhang CX,Zhang Y,Yang SL

更新日期：2009-11-01 00:00:00

abstract：:To explore the feasibility of larch (Larix decidua Mill.) embryogenic cell culture as alternative plant expression system, protein stability in fresh and conditioned medium was characterized in this study and compared to tobacco BY2 suspension culture. Fresh and conditioned media were spiked with 1 microg human IgG an...

journal_title：Biotechnology letters

authors： Calinski A,Classen B,Zoglauer K,Boehm R

更新日期：2009-05-01 00:00:00

abstract：OBJECTIVES:To identify and characterize a novel antimicrobial peptide, catesbeianin-1. RESULTS:Catesbeianin-1 is 25 amino acids long and is α-helical, cationic and amphipathic. It had antimicrobial activity against Gram-positive and Gram-negative bacteria. It was resistant against trypsin and pepsin. Catesbeianin-1 ex...

journal_title：Biotechnology letters

authors： Xu H,Zhang Y,Feng X,Tie K,Cao Y,Han W

更新日期：2017-06-01 00:00:00

abstract：:A chip was developed to store DNA for medical research. The optional restriction site fixed on the chip can randomly ligate with whole human genomic DNA treated by the corresponding restriction enzyme. PCR can then use the chip as template DNA. Moreover, a chip fixing two restriction sites (e.g. EcoRI and HindIII) sho...

journal_title：Biotechnology letters

authors： Hanaoka T,Takai O,Takahashi K,Tsugane S

更新日期：2003-03-01 00:00:00

abstract：:Pancreatic islet transplantation is used to treat diabetes mellitus that has minimal complications and avoids hypoglycemic shock. Conformal microencapsulation of pancreatic islets improves their function by blocking immunogenic molecules while protecting fragile islets. However, production of empty alginate capsules d...

journal_title：Biotechnology letters

authors： Shin S,Yoo YJ

更新日期：2013-12-01 00:00:00

abstract：:Leymus chinensis is an important grassland perennial grass. However, its drought tolerance requires to be improved. LEA (late embryogenesis abundant) genes are believed to confer resistance to drought and water deficiency. Using Agrobacterium-mediated transformation, a wheat LEA gene, TaLEA(3), was integrated into L. ...

journal_title：Biotechnology letters

authors： Wang L,Li X,Chen S,Liu G

更新日期：2009-02-01 00:00:00

abstract：:The use of microRNAs (miRNAs) for improving the efficiency of recombinant protein production by CHO cells is gaining considerable interest for their ability to regulate entire molecular networks. Differential miRNA expression profiling and large-scale transient screening have been the prerequisite for the selection of...

journal_title：Biotechnology letters

authors： Kelly PS,Gallagher C,Clynes M,Barron N

更新日期：2015-04-01 00:00:00

abstract：:KGLP-1, a 31-amino acid glucagon-like peptide-1 (GLP-1) analogue, has a great therapeutic potential for anti-diabetes. In this work, a strategy for expression and purification of functional KGLP-1 peptide has been established. KGLP-1 cDNA was fused with glutathione S-transferase (GST), with an enterokinase cleavage si...

journal_title：Biotechnology letters

authors： Liu Y,Ren L,Ge L,Cui Q,Cao X,Hou Y,Bai F,Bai G

更新日期：2014-08-01 00:00:00

abstract：:The open reading frame of the Δ(6)-desaturase gene was isolated from Mortierella alpina W15 and the gene was cloned into a pPIC3.5K vector. The vector was transformed into Pichia pastoris GS115 and expression was induced with methanol. The Δ(6)-desaturase expressed in P. pastoris GS115 catalyzed the conversion of lino...

journal_title：Biotechnology letters

authors： Liu J,Li D,Yin Y,Wang H,Li M,Yu L

更新日期：2011-10-01 00:00:00

abstract：OBJECTIVES:To improve heterologous proteins production, we constructed a maltose-inducible expression system in Bacillus subtilis. RESULTS:An expression system based on the promoter for maltose utilization constructed in B. subtilis. Successively, to improve the performance of the P malA -derived system, mutagenesis w...

journal_title：Biotechnology letters

authors： Yue J,Fu G,Zhang D,Wen J

更新日期：2017-08-01 00:00:00

abstract：:δ-Integration can improve the expression stability and increase the copy number of exogenous genes in Saccharomyces cerevisiae. Generally, δ-integration vectors employ auxotrophic markers, such as LEU2-d, HIS3, TRP1, and URA3, to screen transformants. In this study, two cellulase genes (β-glucosidase and endoglucanase...

journal_title：Biotechnology letters

authors： Liu L,Liu C,Zou S,Yang H,Hong J,Ma Y,Zhang M

更新日期：2013-08-01 00:00:00

abstract：:The penicillin G amidase (PGA) from Alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in Escherichia coli. The corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter....

journal_title：Biotechnology letters

authors： Deak PM,Lutz-Wahl S,Bothe H,Fischer L

更新日期：2003-03-01 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) assay system was employed for detecting Bacillus anthracis spores in pure cultures as well as in various simulated powder samples. The specificity of the designed LAMP primer sets was validated by assaying 13 B. anthracis strains and 33 non-B. anthracis species. The dete...

journal_title：Biotechnology letters

authors： Qiao YM,Guo YC,Zhang XE,Zhou YF,Zhang ZP,Wei HP,Yang RF,Wang DB

更新日期：2007-12-01 00:00:00

abstract：OBJECTIVE:The effect of epicatechin-3-gallate (ECG), a polyphenol that is present in green tea, on doxorubicin (DOX) cytotoxicity in H9C2 cardiomyocytes and its underlying mechanisms were investigated. RESULTS:Pretreatment with ECG (20 and 30 μM) significantly increased DOX-induced apoptosis to 16-18% in H9C2 cardiomy...

journal_title：Biotechnology letters

authors： Cai Y,Hong HQ,Zhao L,Cai YP,Qin Y

更新日期：2015-10-01 00:00:00

abstract：:A strain of Serratia sp. showed intracellular electron-transparent inclusion bodies when incubated in the presence of citrate and glycerol 2-phosphate without nitrogen source following pre-growth under carbon-limitation in continuous culture. About 1.3 mmol citrate were consumed per 450 mg biomass, giving a calculated...

journal_title：Biotechnology letters

authors： Lugg H,Sammons RL,Marquis PM,Hewitt CJ,Yong P,Paterson-Beedle M,Redwood MD,Stamboulis A,Kashani M,Jenkins M,Macaskie LE

更新日期：2008-03-01 00:00:00

abstract：:A single-compartmented microbial fuel cell composed of a graphite felt anode modified with Neutral Red (NR-anode) and a porous Fe(II)-carbon cathode (FeC-cathode) were compared for electricity generation from Microbacterium sp. and Pseudomonas sp. under identical conditions. Pseudomonas sp. was more than four times th...

journal_title：Biotechnology letters

authors： Lee SW,Jeon BY,Park DH

更新日期：2010-04-01 00:00:00

abstract：:An integrative vector was constructed for inserting heterologous genes within a non-functional open reading frame (ORF) on the chromosome of Streptococcus thermophilus. The vector, pINTRS, contained a temperature sensitive origin of replication and an erythromycin resistance gene for initial selection in S. thermophil...

journal_title：Biotechnology letters

authors： Renye JA Jr,Somkuti GA

更新日期：2009-05-01 00:00:00

abstract：:N(omega)-2,2,4,6,7-Pentamethyldihydrobenzofuran-5-sulfonyl (N(omega)-Pbf)-protected oligoarginine was directly conjugated to poly(amino acid) derivatives modified with a long alkyl chain. The final concentration of conjugated peptides was easily controlled by the feed ratio of oligoarginine to polymer backbone and a f...

journal_title：Biotechnology letters

authors： Yang SR,Kim SB,Joe CO,Kim JD

更新日期：2005-07-01 00:00:00

abstract：:Batch fermentation by Streptomyces pristinaespiralis with the addition of adsorbent resins was used to increase the production of pristinamycin. In consideration of the adsorption capacity and the desorption ability, a polymeric resin, JD-1, was finally selected. The maximum production of pristinamycin in Erlenmeyer f...

journal_title：Biotechnology letters

authors： Jia B,Jin ZH,Lei YL,Mei LH,Li NH

更新日期：2006-11-01 00:00:00

abstract：:We truncated the VP2 protein of infectious bursal disease virus into five fragments: V1-5. All fragments were displayed on the inner membrane of the Escherichia coli periplasm. After disruption of the outer membrane, spheroplasts that had anchored with the VP2 fragment were incubated with an anti-VP2 polyclonal antibo...

journal_title：Biotechnology letters

authors： Guo M,Xu LM,Zhou B,Yin JC,Ye XL,Ren GP,Li DS

更新日期：2014-03-01 00:00:00

abstract：:beta-Galactosidase isolated from Aspergillus oryzae was immobilized in lens-shaped polyvinylalcohol capsules (with activity 25 U g(-1)) giving 32% of its original activity. Immobilization did not change the pH optimum (4.5) of lactose hydrolysis. The relative enzyme activity during product inhibition testing was, in a...

journal_title：Biotechnology letters

authors： Grosová Z,Rosenberg M,Rebros M,Sipocz M,Sedlácková B

更新日期：2008-04-01 00:00:00