Abstract:
:beta-Galactosidase isolated from Aspergillus oryzae was immobilized in lens-shaped polyvinylalcohol capsules (with activity 25 U g(-1)) giving 32% of its original activity. Immobilization did not change the pH optimum (4.5) of lactose hydrolysis. The relative enzyme activity during product inhibition testing was, in average, 10% higher for immobilized enzyme. No decrease of activity was observed after 35 repeated batch runs and during 530 h of continuous hydrolysis of lactose (10%, w/v) at 45 degrees C. The immobilized enzyme was stable for 14 months without any change of activity during the storage at 4 degrees C and pH 4.5.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Grosová Z,Rosenberg M,Rebros M,Sipocz M,Sedlácková Bdoi
10.1007/s10529-007-9606-0subject
Has Abstractpub_date
2008-04-01 00:00:00pages
763-7issue
4eissn
0141-5492issn
1573-6776journal_volume
30pub_type
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