Foam mitigation and exploitation in biosurfactant production.

abstract：:Image analysis was used to measure the effect of Tween 40 on the morphology of Trichoderma harzianum. The percentage of pellets was maximal (93%) with Tween at 0.2 ml l(-1), whereas the maximal proportion of dispersed mycelia (40%) was with Tween at 1.6 ml l(-1). The particle median diameter decreased from 2 to 0.5 mm...

journal_title：Biotechnology letters

authors： Lucatero S,Galindo E,Larralde-Corona CP

更新日期：2004-01-01 00:00:00

abstract：:A dicistronic expression vector was constructed for Chinese hamster ovary (CHO) cells that produce both selectable marker-DHFR (dihydrofolate reductase) gene and recombinant antibody cDNA from a single primary transcript via differential splicing. The vector was derived from a pDHL vector and contained the human const...

journal_title：Biotechnology letters

authors： Xiong KH,Liang QC,Xiong H,Zou CX,Gao GD,Zhao ZW,Zhang H

更新日期：2005-11-01 00:00:00

abstract：:Salinispora arenicola CNS-205 was a first-isolated obligate marine actinomycete. A gene (sare0357), annotated as ''amino acid adenylation domain'' located on the genome of Salinispora arenicola CNS-205, was cloned and characterized. The recombinant target protein Sare0357 was expressed in E. coli. Sare0357 specificall...

journal_title：Biotechnology letters

authors： Shu X,Ma Y,Lu C,Lei M,Liu Y,Liu K,Xiong G,Xia S,Zhao Z,Luo W,Fu Q,Qi C

更新日期：2015-05-01 00:00:00

abstract：:Monascus aurantiacus produces high amounts of citrinin which is a mycotoxin with nephrotoxic activity. Six putative citrinin biosynthesis genes have been discovered in M. purpureus and at least 10 genes are responsible for its biosynthesis. However, the sequence of citrinin pathway gene cluster in M. aurantiacus has n...

journal_title：Biotechnology letters

authors： Li YP,Xu Y,Huang ZB

更新日期：2012-01-01 00:00:00

abstract：:At the core of a biomanufacturing process for recombinant proteins is the production cell line. It influences the productivity and product quality. Its characteristics also dictate process development, as the process is optimized to complement the producing cell to achieve the target productivity and quality. Advances...

journal_title：Biotechnology letters

authors： Le H,Vishwanathan N,Jacob NM,Gadgil M,Hu WS

更新日期：2015-08-01 00:00:00

abstract：:To understand how molecular damage under harsh environmental conditions can be controlled, we investigated the properties of ATPase activity of the chaperonin molecular machinery from the hyperthermophilic archaeon Pyrococcus furiosus (PfCPN). PfCPN ATPase activity depended on K(+) and Mg(2+) and its optimal pH was 7....

journal_title：Biotechnology letters

authors： Chen HY,Tan XL,Lu J,Zhang CX,Zhang Y,Yang SL

更新日期：2009-11-01 00:00:00

abstract：:The genetic relationship among commercial cultivars of Citrus limon (lemon) was analysed by inter-simple sequence repeats (ISSR) and flow cytometry techniques. Two cultivars with a close germplasm were distinguished by screening 10 SSR primers and by measuring DNA content of prestained nuclei. ...

journal_title：Biotechnology letters

authors： Capparelli R,Viscardi M,Amoroso MG,Blaiotta G,Bianco M

更新日期：2004-08-01 00:00:00

abstract：:Many enzymes used as digestive aids exhibit, at best, moderate stability when incubated under gastrointestinal conditions. A supplemental β-galactosidase administered orally to treat lactose intolerance was conjugated to 40 kDa, branched polyethylene glycol (PEG). PEGylation increased the enzyme's relative activity at...

journal_title：Biotechnology letters

authors： Turner KM,Pasut G,Veronese FM,Boyce A,Walsh G

更新日期：2011-03-01 00:00:00

abstract：:Glucosylceramide and galactosylceramide were detected in three Aspergillus species: Aspergillus oryzae, Aspergillus sojae and Aspergillus. awamori, using borate-coated TLC. The cerebrosides from A. oryzae were further purified by ion exchange and iatrobeads column chromatographies with or without borate, and determine...

journal_title：Biotechnology letters

authors： Tani Y,Amaishi Y,Funatsu T,Ito M,Itonori S,Hata Y,Ashida H,Yamamoto K

更新日期：2014-12-01 00:00:00

abstract：:A Simplified version of rate equations for enzyme-catalyzed reactions has been developed in which the rate equation is analyzed with two different mechanisms: ordered bi-ter and Ping-Pong bi-bi mechanisms. This procedure is able to develop the rate expressions accurately. Random sequential order mechanism can be effec...

journal_title：Biotechnology letters

authors： Thilakavathi M,Basak T,Panda T

更新日期：2006-12-01 00:00:00

abstract：:A novel glucose-sensing molecule was created based on galactose/glucose-binding protein (GGBP). GGBP mutants at Asp14, a residue interacting with the 4th hydroxyl group of the sugar molecule, were constructed by mutagenesis to improve the ligand specificity of GGBP. The autofluorescence-based analysis of the binding a...

journal_title：Biotechnology letters

authors： Sakaguchi-Mikami A,Taneoka A,Yamoto R,Ferri S,Sode K

更新日期：2008-08-01 00:00:00

abstract：OBJECTIVE:Silk sericin is a natural polymer with potential utility in biomedical and biotechnological applications. Recombinantly expressed sericin ensures a source of pure protein with no contamination and with multiple properties when expressed as a fusion protein. Hence, the present paper aims to recombinantly expre...

journal_title：Biotechnology letters

authors： Thomas DS,Manoharan C,Rasalkar S,Mishra RK,Gopalapillai R

更新日期：2020-09-01 00:00:00

abstract：:A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min(-1) (mg powder)(-1) in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4 degrees C. Solid-state buffers (bases and their HCl salts) suspended in the organic medium d...

journal_title：Biotechnology letters

authors： Theppakorn T,Kanasawud P,Halling PJ

更新日期：2004-01-01 00:00:00

abstract：:Growth of Ruta graveolens shoots was induced when Bacillus sp. cell lysates were added to the culture medium. Elicitation of coumarin by this lysate was also very effective; the concentrations of isopimpinelin, xanthotoxin and bergapten increased to 610, 2120 and 1460 microg g(-1) dry wt, respectively. It also had a s...

journal_title：Biotechnology letters

authors： Orlita A,Sidwa-Gorycka M,Malinski E,Czerwicka M,Kumirska J,Golebiowski M,Lojkowska E,Stepnowski P

更新日期：2008-03-01 00:00:00

abstract：:Angiogenin, a potent angiogenic factor, was cloned and expressed by Escherichia coli and then purified with gel filtration chromatography. Approximately 90% pure angiogenin was obtained to generate a monoclonal antibody. Using western immunoblotting and ELISA, we confirmed that monoclonal antibody C46 secreted from hy...

journal_title：Biotechnology letters

authors： Wu J,Zhang HB,Wang J,Yang TC,Xian J,Yang CH,Lai HW

更新日期：2008-06-01 00:00:00

abstract：:The thermostablility and enzymatic activity of 1,3-1,4-β-glucanase (BglA) from Bacillus amyloliquefaciens was improved by modifying five (out of 12) ε-amino groups in lysine residues with nitrous acid. The optimal modification condition for BglA was determined as 30 mM nitrous acid at, 40 °C for 30 min. The optimally-...

journal_title：Biotechnology letters

authors： Niu C,Zhu L,Wang J,Li Q

更新日期：2014-12-01 00:00:00

abstract：:Deacetoxy/deacetylcephalosporin C synthase (acDAOC/DACS) from Acremonium chrysogenum is a bifunctional enzyme that catalyzes both the ring-expansion of penicillin N to deacetoxycephalosporin C and the hydroxylation of the latter to deacetylcephalosporin C. The R308 residue located in close proximity to the C-terminus ...

journal_title：Biotechnology letters

authors： Wu XB,Tian XY,Ji JJ,Wu WB,Fan KQ,Yang KQ

更新日期：2011-04-01 00:00:00

abstract：OBJECTIVES:To design and construct a novel bio-anode electrode based on the oxidation of glutamic acid to produce 2-oxoglutarate, generating two electrons from NADH. RESULTS:Efficient enzyme reaction and electron transfer were observed owing to immobilization of the two enzymes using a mixed self-assembled monolayer. ...

journal_title：Biotechnology letters

authors： Sakamoto H,Komatsu T,Yamasaki K,Satomura T,Suye SI

更新日期：2017-02-01 00:00:00

abstract：OBJECTIVE:In this study, we aimed to maximize glutathione (GSH) production by a metabolically engineered Yarrowia lipolytica strain using a small-scale optimization approach. RESULTS:A three levels four factorial Box-Behnken Design was used to assess the effect of pH, inulin extract, yeast extract and ammonium sulfate...

journal_title：Biotechnology letters

authors： Do DTH,Fickers P,Ben Tahar I

更新日期：2021-02-01 00:00:00

abstract：:The lipase of Pseudomonas cepacia was immobilized onto electrospun polyacrylonitrile (PAN) fibers and used for the conversion of (S)-glycidol with vinyl n-butyrate to glycidyl n-butyrate in isooctane. The rate of reaction with the adsorbed lipase was 23-fold higher than the initial material. After 10 recyclings, the i...

journal_title：Biotechnology letters

authors： Sakai S,Liu Y,Yamaguchi T,Watanabe R,Kawabe M,Kawakami K

更新日期：2010-08-01 00:00:00

abstract：:A strain of Pseudomonas stutzeri ZWLR2-1 was isolated from soil contaminated with chloronitrobenzenes and identified by 16S rDNA sequencing. This bacterium released chloride and nitrite into the medium when grown on 0.5 mM 2-chloronitrobenzene. PCR amplification and DNA sequencing revealed a DNA fragment encoding a po...

journal_title：Biotechnology letters

authors： Liu H,Wang SJ,Zhou NY

更新日期：2005-02-01 00:00:00

abstract：:Electro-acupuncture (EA) at Fenglong acupoint (ST40) can lower the levels of serum cholesterol and triacylglycerols. To study the hepatic genes responsible for the cholesterol-lowering effect of EA, suppression subtractive hybridization combined with the switch mechanism at the 5'-end of RNA template cDNA synthesis an...

journal_title：Biotechnology letters

authors： Li L,Tan GH,Zhang YZ

更新日期：2012-07-01 00:00:00

abstract：:Brevibacterium linens forms hydrolytic enzymes which can be used to accelerate the ripening of cheese without causing bitterness. B. linens ATCC 9172 was grown to a high cell density (50 g dry wt l-1 after 60 h) in a mineral medium containing lactic acid, soy-peptone and ammonium sulphate by applying a continuous feed...

journal_title：Biotechnology letters

authors： Adamitsch BF,Karner F,Hampel WA

更新日期：2003-05-01 00:00:00

abstract：:We proposed a novel combined gene therapy of human vascular endothelial growth factor 165 gene (hVEGF165) and human bone morphogenetic protein 2 gene (hBMP2) for bone regeneration by lentivirus-mediated co-transfection of both genes into rat bone marrow-derived mesenchymal stromal cells (MSCs). Both genes were success...

journal_title：Biotechnology letters

authors： Jiang J,Fan CY,Zeng BF

更新日期：2008-02-01 00:00:00

abstract：:Two partial amphiploid lines, B113 (32 plants) and B21 (13 plants), derived from a wheat-Psathyrostachys huashanica intergeneric cross were characterized by Giemsa C-banding and SDS-PAGE and evaluated for stripe rust resistance. All 15 partial amphiploid plants were aneuploids with either 50 (8 plants), 51 (6 plants) ...

journal_title：Biotechnology letters

authors： Wang Y,Xie Q,Yu K,Poysa V,Lin L,Kang H,Fan X,Sha L,Zhang H,Zhou Y

更新日期：2011-06-01 00:00:00

abstract：:Cell-free extracts of Streptomyces sp. DRS I converted ampicillin to cephalexin, presumably due to the activity of the enzyme, expandase. The extract was fractionated and characterized by colorimetric and chromatographic measurements coupled with disc-agar diffusion bioassay against an ampicillin-resistant, cephalexin...

journal_title：Biotechnology letters

authors： Thakur D,Roy MK,Bora TC

更新日期：2009-07-01 00:00:00

abstract：:An endoxylanase gene, xynA, was cloned from Bacillus pumilus ARA and expressed in Escherichia coli. The open reading frame of the xynA gene was 687 bp encoding a signal peptide and a mature xylanase with a molecular mass of 23 kDa. The enzyme was categorized as a glycosyl hydrolase family 11 member based on the sequen...

journal_title：Biotechnology letters

authors： Qu W,Shao W

更新日期：2011-07-01 00:00:00

abstract：:Biotransformation of 1,3-dichloro-2-propanol (DCP) to epichlorohydrin (ECH) by the whole cells of recombinant Escherichia coli expressing halohydrin dehalogenase was limited by product inhibition. To solve this problem and improve the ECH yield, a biotransformation strategy using resin-based in situ product removal (I...

journal_title：Biotechnology letters

authors： Zou SP,Du EH,Hu ZC,Zheng YG

更新日期：2013-06-01 00:00:00

abstract：:A single-compartmented microbial fuel cell composed of a graphite felt anode modified with Neutral Red (NR-anode) and a porous Fe(II)-carbon cathode (FeC-cathode) were compared for electricity generation from Microbacterium sp. and Pseudomonas sp. under identical conditions. Pseudomonas sp. was more than four times th...

journal_title：Biotechnology letters

authors： Lee SW,Jeon BY,Park DH

更新日期：2010-04-01 00:00:00

abstract：:A hybrid baculovirus, a hybrid of the Autographa californica nuclear polyhedrosis virus and the Bombyx mori nuclear polyhedrosis virus, was used for the large-scale production of bovine interleukin-21 (IL-21) in silkworms. A recombinant hybrid baculovirus containing the full length of the cDNA of bovine interleukin-21...

journal_title：Biotechnology letters

authors： Muneta Y,Nagaya H,Minagawa Y,Enomoto C,Matsumoto S,Mori Y

更新日期：2004-09-01 00:00:00