Simplified approach for developing the rate expressions for enzyme-catalyzed reactions.

abstract：OBJECTIVES:To characterize a novel endoglucanase, Celal_2753, from the psychrophilic bacterium Cellulophaga algicola IC166(T). RESULTS:Celal_2753 was purified to homogeneity with a yield of 81 % and with a molecular weight of 40 kDa on SDS-PAGE. It had maximum hydrolytic activity towards carboxymethyl cellulose at 40 ...

journal_title：Biotechnology letters

authors： Wang Y,Yu W,Han F

更新日期：2016-02-01 00:00:00

abstract：OBJECTIVE:Myelin oligodendrocyte glycoprotein (MOG) is one of the major autoantigens in multiple sclerosis (MS), therefore selective depletion of autoreactive lymphocytes exposing MOG-specific B cell receptors (BCRs) would be beneficial in terms of MS treatment. RESULTS:Using E. coli we generated an efficient protocol...

journal_title：Biotechnology letters

authors： Stepanov A,Belyy A,Kasheverov I,Rybinets A,Dronina M,Dyachenko I,Murashev A,Knorre V,Sakharov D,Ponomarenko N,Tsetlin V,Tonevitsky A,Deyev S,Belogurov A Jr,Gabibov A

更新日期：2016-07-01 00:00:00

abstract：OBJECTIVES:To investigate gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8, a potential high-ethanol producer, in response to various stresses during high-temperature ethanol fermentation using sweet sorghum juice (SSJ) under optimal conditions. RESULTS:The maximal ethanol c...

journal_title：Biotechnology letters

authors： Techaparin A,Thanonkeo P,Klanrit P

更新日期：2017-10-01 00:00:00

abstract：OBJECTIVE:To examine the role of a gene encoding flavin-containing monooxygenase (cFMO) from Corynebacterium glutamicum ATCC13032 when cloned and expressed in Escherichia coli for the production of indigo pigments. RESULTS:The blue pigments produced by recombinant E. coli were identified as indigo and indirubin. The c...

journal_title：Biotechnology letters

authors： Ameria SP,Jung HS,Kim HS,Han SS,Kim HS,Lee JH

更新日期：2015-08-01 00:00:00

abstract：:Electro-acupuncture (EA) at Fenglong acupoint (ST40) can lower the levels of serum cholesterol and triacylglycerols. To study the hepatic genes responsible for the cholesterol-lowering effect of EA, suppression subtractive hybridization combined with the switch mechanism at the 5'-end of RNA template cDNA synthesis an...

journal_title：Biotechnology letters

authors： Li L,Tan GH,Zhang YZ

更新日期：2012-07-01 00:00:00

abstract：:β-Xylosidase from Thermoanaerobacterium thermosaccharolyticum was purified by His-trap affinity chromatography giving a specific activity of 5.15 U mg(-1). From gel-filtration chromatography, the purified enzyme was a tetramer with a total molecular mass of 245 kDa. Maximal enzyme activity using o-nitrophenyl(NP)-β-D-...

journal_title：Biotechnology letters

authors： Shin KC,Seo MJ,Oh DK

更新日期：2014-11-01 00:00:00

abstract：:The effects of glycerol and the oxygen transfer rate on the xylitol production rate by a xylitol dehydrogenase gene (XYL2)-disrupted mutant of Candida tropicalis were investigated. The mutant produced xylitol near the almost yield of 100% from D: -xylose using glycerol as a co-substrate for cell growth and NADPH regen...

journal_title：Biotechnology letters

authors： Ko BS,Rhee CH,Kim JH

更新日期：2006-08-01 00:00:00

abstract：:Two partial amphiploid lines, B113 (32 plants) and B21 (13 plants), derived from a wheat-Psathyrostachys huashanica intergeneric cross were characterized by Giemsa C-banding and SDS-PAGE and evaluated for stripe rust resistance. All 15 partial amphiploid plants were aneuploids with either 50 (8 plants), 51 (6 plants) ...

journal_title：Biotechnology letters

authors： Wang Y,Xie Q,Yu K,Poysa V,Lin L,Kang H,Fan X,Sha L,Zhang H,Zhou Y

更新日期：2011-06-01 00:00:00

abstract：:Methods for the preparation of carrier-free insoluble enzymes are reviewed. The technology of cross-linked enzyme aggregates has now been applied to a range of synthetically useful activities. Fusion proteins are also gaining momentum because they allow a relatively selective aggregation or even a specific self-assemb...

journal_title：Biotechnology letters

authors： Roessl U,Nahálka J,Nidetzky B

更新日期：2010-03-01 00:00:00

abstract：OBJECTIVES:To develop a colorimetric assay for ATP based on the blue-pigment synthesising non-ribosomal peptide synthetase (NRPS) BpsA, and to demonstrate its utility in defining the substrate specificity of other NRPS enzymes. RESULTS:BpsA is able to convert two molecules of L-glutamine into the readily-detected blue...

journal_title：Biotechnology letters

authors： Brown AS,Calcott MJ,Collins VM,Owen JG,Ackerley DF

更新日期：2020-12-01 00:00:00

abstract：:Over-expression of the gene, mshA, coding for mycothiol glycosyl transferase improved the robustness of Corynebacterium glutamicum to various stresses. Intracellular mycothiol (MSH) content was increased by 114 % in WT(pXMJ19-mshA) compared to WT(pXMJ19). Survival rates increased by 44, 39, 90, 77, 131, 87, 52, 47, 57...

journal_title：Biotechnology letters

authors： Liu YB,Chen C,Chaudhry MT,Si MR,Zhang L,Wang Y,Shen XH

更新日期：2014-07-01 00:00:00

abstract：:Error-prone PCR was used to create more thermoactive and/or thermostable variants of thermoalkalophilic lipases. A variant of the α6 helix (lid domain), with an 189E to V substitution at residue 189, lost its thermostability but exhibited higher activity than that of its wild-type predecessor (r03Lip). Site-saturation...

journal_title：Biotechnology letters

authors： Shih TW,Pan TM

更新日期：2011-09-01 00:00:00

abstract：:We assessed the whole genome amplification strategy, known as multiple displacement amplification (MDA), for use with the TaqMan genotyping platform for DNA samples derived from two case-control studies nested in the Nurses' Health Study and the Physicians' Health Study. Our objectives were to (1) quantify DNA yield f...

journal_title：Biotechnology letters

authors： Tranah GJ,Lescault PJ,Hunter DJ,De Vivo I

更新日期：2003-07-01 00:00:00

abstract：:To understand the mechanism of phosphate accumulation, a gene encoding polyphosphate kinase (PPK) was cloned from the genomic library of Serratia marcescens by Southern hybridization. From the nucleotide sequence of a 4 kb DNA fragment, an open reading frame of 2063 nucleotides was identified encoding a protein of 686...

journal_title：Biotechnology letters

authors： Lee SJ,Song OR,Lee YC,Choi YL

更新日期：2003-02-01 00:00:00

abstract：:The inhibition of protein-tyrosine phosphatase 1B (PTP1B) is a potential target for treatment of type 2 diabetes. Vanadium and zinc metal coordinated complexes have insulin-enhancing activities, and while vanadium compounds inhibit PTP1B, little is known on the mode of action of zinc compounds. In this study we develo...

journal_title：Biotechnology letters

authors： Seale AP,de Jesus LA,Kim SY,Choi YH,Lim HB,Hwang CS,Kim YS

更新日期：2005-02-01 00:00:00

abstract：:Electroporation of Litopenaeus schmitti embryos was used to transfer the pE300tiGH15 plasmid that contains the tilapia growth hormone gene (tiGH) complexed with a nuclear localization signal peptide into the zygotes. The gene construct was detected in 35 (36%) of the 98 larvae screened by PCR and Southern blot analyse...

journal_title：Biotechnology letters

authors： Arenal A,Pimentel R,Pimentel E,Martín L,Santiesteban D,Franco R,Aleström P

更新日期：2008-05-01 00:00:00

abstract：:A sigma 70-like gene, sigA, has been identified from Corynebacterium ammoniagenes. The sigA gene encodes a polypeptide of 467 amino acids with a calculated molecular mass of 52036 Da. The deduced amino acid sequence preserves the common motifs of the primary sigma factors and shows very high similarity to those of Sig...

journal_title：Biotechnology letters

authors： Lee BR,Kim JH

更新日期：2004-04-01 00:00:00

abstract：:In order to treat the kitchen wastes and produce hydrogen, anaerobic fermentation technology was used in this experiment. The results showed that the fermentation type changed from mixed acid fermentation to ethanol fermentation in a continuous stirred tank reactor (CSTR) 22 days after start-up. The maximum efficiency...

journal_title：Biotechnology letters

authors： Shi Y,Zhao XT,Cao P,Hu Y,Zhang L,Jia Y,Lu Z

更新日期：2009-09-01 00:00:00

abstract：OBJECTIVES:To display a recombinant avidin fused to the autotransporter ShdA to bind biotinylated molecules on the surface of Escherichia coli. RESULTS:Two chimeric protein constructs containing avidin fused to the autotransporter ShdA were expressed on the surface of Escherichia coli DH5α. One fusion protein containe...

journal_title：Biotechnology letters

authors： Pardavé-Alejandre HD,Alvarado-Yaah JE,Pompa-Mera EN,Muñoz-Medina JE,Sárquiz-Martínez B,Santacruz-Tinoco CE,Manning-Cela RG,Ortíz-Navarrete V,López-Macías C,González-Bonilla CR

更新日期：2018-03-01 00:00:00

abstract：OBJECTIVES:To establish an automated high-throughput mimic perfusion scale-down model (SDM) in ambr® 15 system. RESULTS:An optimized SDM for mimic perfusion was developed in ambr® 15 system. Cell retention in ambr® 15 was realized by sedimentation and supernatant removal with a retention rate > 95%. Although the SDM c...

journal_title：Biotechnology letters

authors： Jin L,Wang ZS,Cao Y,Sun RQ,Zhou H,Cao RY

更新日期：2021-02-01 00:00:00

abstract：OBJECTIVES:To investigate the effects of operational process conditions on expression of MHC class II protein from a stable Drosophila S2 cell line. RESULTS:When the Drosophila S2 cells were grown in vented orbitally shaken TubeSpin bioreactor 600 containers, cell growth was improved three-fold and the yield of recomb...

journal_title：Biotechnology letters

authors： Shen X,Dojcinovic D,Baldi L,Hacker DL,Luescher IF,Wurm FM

更新日期：2018-01-01 00:00:00

abstract：:The ability of Aspergillus strains to utilize thin stillage to produce malic acid was compared. The highest malic acid was produced by Aspergillus niger ATCC 9142 at 17 g l(-1). Biomass production from thin stillage was similar with all strains but ATCC 10577 was the highest at 19 g l(-1). The highest malic acid yield...

journal_title：Biotechnology letters

authors： West TP

更新日期：2011-12-01 00:00:00

abstract：:The use of H(2), He and O(2) during batch fermentation of Saccharomyces cerevisiae BRAS291 increased the final intracellular glycogen contents of the cells from 2-fold to 10-fold compared with a gas-free condition, and this depended on the gas applied. Differently, the intracellular trehalose contents increased from 2...

journal_title：Biotechnology letters

authors： Pham TH,Mauvais G,Vergoignan C,De Coninck J,Cachon R,Feron G

更新日期：2008-02-01 00:00:00

abstract：:Metabolic regulation in Escherichia coli was studied in terms of the changes in the expression of the global regulatory genes rpoD, rpoS, soxRS, cra, fadR, iclR and arcA at three different growth phases, in batch culture. The expression of rpoS and several rpoS-dependent metabolic pathway genes, such as tktB, talA, fu...

journal_title：Biotechnology letters

authors： Rahman M,Hasan MR,Shimizu K

更新日期：2008-05-01 00:00:00

abstract：OBJECTIVES:The action modes of an endocellulase, EGA, and its domains (CD9 and CBM3) during enzymatic treatment of cotton fabrics were investigated. RESULTS:EGA, CD9 and CBM3 had the binding capacity to cellulose substrates, of which the filter paper was the substrate with the strongest binding capacity. Analyses of s...

journal_title：Biotechnology letters

authors： Yu M,Qiu Y,Chen W,Zhao F,Shao J

更新日期：2015-08-01 00:00:00

abstract：:Human granulocyte-macrophage colony stimulating factor (hGM-CSF) is used clinically to treat leucopenia typically caused by cancer chemotherapy or radiotherapy. This study used multiple strategies to obtain very high expression levels of OsrhGM-CSF (14 microg/seed) in rice endosperm. Electron micrographs of immunogold...

journal_title：Biotechnology letters

authors： Ning T,Xie T,Qiu Q,Yang W,Zhou S,Zhou L,Zheng C,Zhu Y,Yang D

更新日期：2008-09-01 00:00:00

abstract：:Image analysis was used to measure the effect of Tween 40 on the morphology of Trichoderma harzianum. The percentage of pellets was maximal (93%) with Tween at 0.2 ml l(-1), whereas the maximal proportion of dispersed mycelia (40%) was with Tween at 1.6 ml l(-1). The particle median diameter decreased from 2 to 0.5 mm...

journal_title：Biotechnology letters

authors： Lucatero S,Galindo E,Larralde-Corona CP

更新日期：2004-01-01 00:00:00

abstract：:Dibutyl phthalate (DBP) was more efficiently degraded by cutinase compared to yeast esterase; i.e. almost 80% of initial DBP (500 mg l(-1)) was decomposed within 7.5 h, and nearly 50% of the degraded DBP disappeared within the initial 30 min. The toxicity of the final DBP degradation products were investigated using v...

journal_title：Biotechnology letters

authors： Kim YH,Lee J

更新日期：2005-05-01 00:00:00

abstract：:Environmental factors, such as drought, salinity, extreme temperature, ozone poisoning, metal toxicity etc., significantly affect crops. To study these factors and to design a possible remedy, biological experimental data concerning these crops requires the quantification of gene expression and comparative analyses at...

journal_title：Biotechnology letters

authors： Gul A,Ahad A,Akhtar S,Ahmad Z,Rashid B,Husnain T

更新日期：2016-04-01 00:00:00

abstract：:We truncated the VP2 protein of infectious bursal disease virus into five fragments: V1-5. All fragments were displayed on the inner membrane of the Escherichia coli periplasm. After disruption of the outer membrane, spheroplasts that had anchored with the VP2 fragment were incubated with an anti-VP2 polyclonal antibo...

journal_title：Biotechnology letters

authors： Guo M,Xu LM,Zhou B,Yin JC,Ye XL,Ren GP,Li DS

更新日期：2014-03-01 00:00:00