Abstract:
:To understand the mechanism of phosphate accumulation, a gene encoding polyphosphate kinase (PPK) was cloned from the genomic library of Serratia marcescens by Southern hybridization. From the nucleotide sequence of a 4 kb DNA fragment, an open reading frame of 2063 nucleotides was identified encoding a protein of 686 amino acids with molecular mass of 70 kDa. The potential CRP binding site and pho box sequence were found upstream of the putative promoter in the regulatory region. The expression of PPK resulted in the formation of inclusion bodies and the product was active at low temperature. The E. coli strain harboring plasmid pSPK5 with ppk gene increased enzyme activity of polyphosphate kinase, resulting in increased accumulation of polyphosphate in E. coli.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Lee SJ,Song OR,Lee YC,Choi YLdoi
10.1023/a:1022386514487keywords:
subject
Has Abstractpub_date
2003-02-01 00:00:00pages
191-7issue
3eissn
0141-5492issn
1573-6776journal_volume
25pub_type
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