Abstract:
:A 4 kb BamHI-HindIlI fragment, corresponding to the cry2A operon of Bacillus thuringiensis subsp. kurstaki strain BNS3, was cloned. The sequencing of the corresponding cry2Aa-type gene, termed crybns3-4, revealed an open reading frame of 1902 bp, encoding a protein of 633 amino-acid residues. Both nucleotide and amino-acid sequences similarity analysis revealed that crybns3-4 is a new cry2Aa-type gene which has several differences from the reported cry2Aa-type genes. The transfer of the cloned operon to an acrystalliferous mutant of BNS3, revealed an expression of the new cry2Aa-type gene and a production of parasporal crystal inclusions in the transformants.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Tounsi S,Jaoua Sdoi
10.1023/a:1025016221891keywords:
subject
Has Abstractpub_date
2003-08-01 00:00:00pages
1219-23issue
15eissn
0141-5492issn
1573-6776journal_volume
25pub_type
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journal_title:Biotechnology letters
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journal_title:Biotechnology letters
pub_type: 杂志文章
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journal_title:Biotechnology letters
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更新日期:2016-12-01 00:00:00