Abstract:
OBJECTIVES:The objective of this study was to discover unknown differentially expressed genes (DEGs) associated with bronchopulmonary dysplasia (BPD), analyze their functions and enriched signaling pathways, and identify hub genes correlating with BPD incidence and evolvement. RESULTS:Of 1289 DEGs identified, 568 were downregulated and 721 were upregulated. The DEGs were mainly associated with oxidative stress, angiogenesis, extracellular matrix, inflammation, cell cycle, and protein binding. Eight DEGs were identified as hub genes, including C-X-C motif chemokine ligand 5 (Cxcl5), connective tissue growth factor (Ctgf), interleukin 6 (IL6), matrix metallopeptidase 9 (Mmp9), mitogen-activated protein kinase 14 (Mapk14), platelet and endothelial cell adhesion molecule 1 (Pecam1), TIMP metallopeptidase inhibitor 1 (Timp1), and TIMP metallopeptidase inhibitor 2 (Timp2). IL6 mRNA and protein expression levels were significantly increased in the peripheral blood of neonates with BPD. CONCLUSIONS:Hence, BPD involves complex biological changes. Our findings indicate that inflammation and angiogenesis may play major roles in BPD pathogenesis and that IL6 has the potential to serve as a biomarker for early BPD diagnosis.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Yan W,Jiang M,Zheng Jdoi
10.1007/s10529-020-02986-ysubject
Has Abstractpub_date
2020-12-01 00:00:00pages
2569-2580issue
12eissn
0141-5492issn
1573-6776pii
10.1007/s10529-020-02986-yjournal_volume
42pub_type
杂志文章abstract:OBJECTIVES:Breast cancer is a popular fatal malignant tumor for women with high of rates incidence and mortality. Development of the new approaches for breast cancer targeted diagnosis and chemotherapy is emergently needed by the current clinical practice, the important first step is finding a breast cancer specificall...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03044-3
更新日期:2021-01-01 00:00:00
abstract::Endophytic colonization in rice was induced using rhizobia. Dehusked seeds of rice hybrid, CORH2, were used as explants for induction of calli. MS medium was modified with 2,4-D (2.5 mg l(-1)) and kinetin (0.2 mg l(-1)) for callus induction. Well-developed calli were inoculated with Azorhizobium caulinodans strains OR...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9693-6
更新日期:2008-08-01 00:00:00
abstract:PURPOSE OF WORK:To apply a fluorescent dye as an alternative technique to evaluate the survival of potentially probiotic lactobacilli to bile acids (BA) as first step in the design of probiotic functional foods. The use of lactic acid bacteria (LAB) in the functional food design depends on their ability to survive in t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0564-1
更新日期:2011-07-01 00:00:00
abstract::A 4 kb BamHI-HindIlI fragment, corresponding to the cry2A operon of Bacillus thuringiensis subsp. kurstaki strain BNS3, was cloned. The sequencing of the corresponding cry2Aa-type gene, termed crybns3-4, revealed an open reading frame of 1902 bp, encoding a protein of 633 amino-acid residues. Both nucleotide and amino...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025016221891
更新日期:2003-08-01 00:00:00
abstract::A novel two-step fermentation process was developed to enhance arachidonic acid (ARA) production by Mortierella alpina ME-1 in a 5 l fermentor. Agitation speed and aeration rate were adjusted from 180 to 40 rpm and from 0.6 to 1 vvm, respectively, after 5 days cultivation, to decrease physical damage to the mycelia an...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9661-1
更新日期:2008-06-01 00:00:00
abstract::Fourteen yeast strains were screened for production of 2-phenylethanol from L-phenylalanine with molasses as carbon source. Up to 1 g 2-phenylethanol l-1 was obtained. Using oleyl alcohol as a second phase for in situ product removal to enhance the production of 2-phenylethanol increased the yield to about 3 g 2-pheny...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022890119847
更新日期:2003-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2413-3
更新日期:2017-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1263-x
更新日期:2013-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/B:BILE.0000045629.57791.5a
更新日期:2004-09-01 00:00:00
abstract::1,3-Propanediol production by Klebsiella pneumoniae was studied in batch cultures under N2 flow and four airflow systems. Different byproducts were formed under different aeration conditions. An anaerobic/aerobic combined fed-batch culture was developed giving 70 g 1,3-propanediol l(-1) and 16 g 2,3-butanediol l(-1) w...
journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2004-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022626627231
更新日期:2003-03-01 00:00:00
abstract::Rhizopus arrhizus, strain DAR 36017, produced L(+)-lactic acid in a simultaneous saccharification and fermentation process using starch waste effluents. Lactic acid at 19.5-44.3 g l(-1) with a yield of 0.85-0.96 g g(-1) was produced in 40 h using 20-60 g starch l(-1). Supplementation of nitrogen source may be unnecess...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000004389.53388.d0
更新日期:2003-12-01 00:00:00
abstract::The astaxanthin biosynthesis gene cluster in Paracoccus haeundaensis consists of six genes: crtW, crtZ, crtY, crtI, crtB, and crtE contain 726, 486, 1158, 1503, 912, and 879 base pairs, respectively. Individual carotenoid biosynthesis genes of P. haeundaensis have now been expressed in E. coli and each gene product ha...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9072-0
更新日期:2006-08-01 00:00:00
abstract::An endo-beta-(1-->3),(1-->4)-glucanase gene (bglBC1) from Bacillus circulans ATCC21367 was modified by substituting its native promoter with a strong promoter, BJ27X, to increase expression of the gene when cloned into B. subtilis RM125 and B. megaterium ATCC14945. A 771-bp endo-beta-(1-->3),(1-->4)-glucanase open rea...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-4581-1
更新日期:2004-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2196-y
更新日期:2016-12-01 00:00:00
abstract::A gene, rBmK Cta, encoding a chlorotoxin-like peptide from the scorpion, Buthus martensii Karsch, was synthesized according to the sequence optimized for codon usage in Escherichia coli and was expressed in E. coli BL21 (DE3) using a pExSecI expression system in which the IgG-binding domain-ZZ of protein A is fused to...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2514-2
更新日期:2005-10-01 00:00:00
abstract::Pancreatic islet transplantation is used to treat diabetes mellitus that has minimal complications and avoids hypoglycemic shock. Conformal microencapsulation of pancreatic islets improves their function by blocking immunogenic molecules while protecting fragile islets. However, production of empty alginate capsules d...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1300-9
更新日期:2013-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024192131343
更新日期:2003-07-01 00:00:00
abstract::A recombinant plasmid, pGMF, containing a gamma-glutamylcysteine synthetase gene (GSH-I) from Saccharomyces cerevisiae, was constructed with a copper-resistance gene as the selection marker and was introduced into S. cerevisiae YSF-31. The glutathione content of the recombinant strain was 1.5-fold (13.1 mg g dry cells...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000018261.14427.e8
更新日期:2004-03-01 00:00:00
abstract::Flavonoids have been proposed to play diverse roles in plant growth and development, including defense, symbiosis, pollen development and male fertility, polar auxin transport, and protection against ultraviolet radiation. Recently, a new role in cell cycle regulation has emerged. Genetic alteration of glucuronide met...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-005-1521-7
更新日期:2005-03-01 00:00:00
abstract::An integrative vector was constructed for inserting heterologous genes within a non-functional open reading frame (ORF) on the chromosome of Streptococcus thermophilus. The vector, pINTRS, contained a temperature sensitive origin of replication and an erythromycin resistance gene for initial selection in S. thermophil...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-9931-6
更新日期:2009-05-01 00:00:00
abstract::Acetic acid is by-product from fermentation processes for producing succinic acid using Mannheimia succiniciproducens . To obtain pure succinic acid from the final fermentation broth, acetic acid was selectively removed based on the different extractability of succinic acid and acetic acid with pH using tri-n-octylami...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/B:BILE.0000045656.00138.93
更新日期:2004-10-01 00:00:00
abstract::The proteome of rumen epithelial tissue was analysed by SDS-PAGE coupled with LC-MS/MS. 813 non-redundant proteins were identified of which 7.4 % featured membrane-spanning domains and 15.4 % harboured a signal peptide. According to the gene ontology annotation, the most abundant proteins exhibited binding activities ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1291-6
更新日期:2013-11-01 00:00:00
abstract::The extracellular domains (ECD) of epidermal growth factor receptors, ErbB1, 2, 3 and 4, were designed as soluble dimeric forms. Each ECD was fused to a short hinge region derived from IgG, such that the stable dimer could be formed with disulfide bridges. This hinge-tagged design minimized the molecular weight to app...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0160-9
更新日期:2010-03-01 00:00:00
abstract::Fluctuating light intensity had a more significant impact on growth of gametophytes of transgenic Laminaria japonica in a 2500 ml bubble-column bioreactor than constant light intensity. A fluctuating light intensity between 10 and 110 microE m(-2) s(-1), with a photoperiod of 14 h:10 h light:dark, was the best regime ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-0938-3
更新日期:2005-09-01 00:00:00
abstract:OBJECTIVE:A bio-based process is appealing for purification of L-lactic acid, the major enantiomer of polylactic acid syrup, generated by thermochemical processes at the end of life of PLA-based plastics, from its chiral impurity, D-lactic acid, before reuse. RESULTS:Polylactic acid (PLA), a renewable alternative to p...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1924-z
更新日期:2015-12-01 00:00:00
abstract::In order to treat the kitchen wastes and produce hydrogen, anaerobic fermentation technology was used in this experiment. The results showed that the fermentation type changed from mixed acid fermentation to ethanol fermentation in a continuous stirred tank reactor (CSTR) 22 days after start-up. The maximum efficiency...
journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00
abstract::Chlorella protothecoides, a lutein-producing microalga, was grown aerobically in a mineral medium prepared with 70% (v/v) deuterated water. HPLC/atmospheric pressure chemical ionization-mass spectrometry (HPLC/APCI-MS) analysis revealed approximately 58% replacement of hydrogen by deuterium atoms as indicated by the m...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9105-8
更新日期:2006-09-01 00:00:00
abstract::The baculovirus expression vector system (BEVS) is used extensively for the production of proteins from exogenous cDNAs. However, BEVS is not ideal for pharmaceutical production of glycoproteins owing to the properties of the N-glycans in the expressed products and that insect cells lack several of the enzymes require...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-8107-2
更新日期:2005-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0544-5
更新日期:2011-06-01 00:00:00