L-Lactic acid production from raw cassava starch in a circulating loop bioreactor with cells immobilized in loofa (Luffa cylindrica).

abstract：OBJECTIVES:To investigate the effect of full-length fragment of DNA topoisomerase I gene (TOP1) matrix attachment regions (MARs) originating from the human genome on transgene expression in Chinese hamster ovary (CHO) cells and explore the underlying mechanisms. RESULTS:Results showed that TOP1 MAR cannot only enhance...

journal_title：Biotechnology letters

authors： Jia YL,Guo X,Wang XC,Wang TY

更新日期：2019-07-01 00:00:00

abstract：:Classical purification of the glycoprotein equine chorionic gonadotropin (eCG) from serum includes pH fractionation with metaphosphoric acid, two ethanol precipitation steps as well as dialysis followed by fixed-bed chromatography. A simplified process requiring only 1/3 of the solvent and improving the yield from 53 ...

journal_title：Biotechnology letters

authors： Müller C,Wagner K,Frankenfeld K,Franzreb M

更新日期：2011-05-01 00:00:00

abstract：:Organophosphorus hydrolase (OPH) hydrolyzes organophosphorus esters. We constructed the yeast-displayed OPH using Flo1p anchor system. In this system, the N-terminal region of the protein was fused to Flo1p and the fusion protein was displayed on the cell surface. Hydrolytic reactions with paraoxon were carried out du...

journal_title：Biotechnology letters

authors： Fukuda T,Tsuchiyama K,Makishima H,Takayama K,Mulchandani A,Kuroda K,Ueda M,Suye S

更新日期：2010-05-01 00:00:00

abstract：OBJECTIVE:Silk sericin is a natural polymer with potential utility in biomedical and biotechnological applications. Recombinantly expressed sericin ensures a source of pure protein with no contamination and with multiple properties when expressed as a fusion protein. Hence, the present paper aims to recombinantly expre...

journal_title：Biotechnology letters

authors： Thomas DS,Manoharan C,Rasalkar S,Mishra RK,Gopalapillai R

更新日期：2020-09-01 00:00:00

abstract：:Heterologous expression in Escherichia coli often leads to production of the expressed proteins as insoluble and inactive inclusion bodies. The general strategy for protein recovery includes isolation and washing of inclusion bodies, solubilization of aggregated protein and refolding of solubilized protein. The proces...

journal_title：Biotechnology letters

authors： Ferreira S,Sousa F,Queiroz JA,Domingues FC

更新日期：2005-08-01 00:00:00

abstract：:The Rho1 protein is a GTPase that participates in cell wall biogenesis. We analyzed the transcript levels of laccase genes (lccl, lcc2, lcc3, lcc4, lcc5, and lcc9), and a xylanase gene (xyl3) in Fusarium oxysporum f. sp. lycopersici strain 4287 (wild type) and two mutant strains; rhol::hyg that lacks a functional Rho1...

journal_title：Biotechnology letters

authors： Reyes-Medina MA,Macías-Sánchez KL

更新日期：2015-03-01 00:00:00

abstract：:An extracellular chitinase from Streptomyces halstedii AJ-7, a broad spectrum antifungal biocontrol agent, was characterized and purified. The apparent molecular weight of the purified protein was 55 kDa, Km value and Vmax of the protein for colloidal chitin were 3.2 mg ml(-1) and 118 micromol h(-1), respectively. The...

journal_title：Biotechnology letters

authors： Joo GJ

更新日期：2005-10-01 00:00:00

abstract：:Electroporation of Litopenaeus schmitti embryos was used to transfer the pE300tiGH15 plasmid that contains the tilapia growth hormone gene (tiGH) complexed with a nuclear localization signal peptide into the zygotes. The gene construct was detected in 35 (36%) of the 98 larvae screened by PCR and Southern blot analyse...

journal_title：Biotechnology letters

authors： Arenal A,Pimentel R,Pimentel E,Martín L,Santiesteban D,Franco R,Aleström P

更新日期：2008-05-01 00:00:00

abstract：:Avian myeloblastosis virus reverse transcriptase (AMV RT) is a heterodimer consisting of a 63 kDa α-subunit and a 95 kDa β subunit. Moloney murine leukaemia virus reverse transcriptase (MMLV RT) is a 75 kDa monomer. These two RTs are the most extensively used for conversion of RNA to DNA. We previously developed sever...

journal_title：Biotechnology letters

authors： Konishi A,Yasukawa K,Inouye K

更新日期：2012-07-01 00:00:00

abstract：:Image analysis was used to measure the effect of Tween 40 on the morphology of Trichoderma harzianum. The percentage of pellets was maximal (93%) with Tween at 0.2 ml l(-1), whereas the maximal proportion of dispersed mycelia (40%) was with Tween at 1.6 ml l(-1). The particle median diameter decreased from 2 to 0.5 mm...

journal_title：Biotechnology letters

authors： Lucatero S,Galindo E,Larralde-Corona CP

更新日期：2004-01-01 00:00:00

abstract：:A new technique for the direct production of recombinant baculovirus in the silkworm larvae is described. To assess the utility of this method, a combination of Bombyx mori nucleopolyhedroviral genome, transfer vector and Lipofectin was co-injected directly into newly ecdysed fifth instar, silkworm larvae. The recombi...

journal_title：Biotechnology letters

authors： Lee KS,Kim BY,Je YH,Woo SD,Sohn HD,Jin BR

更新日期：2007-01-01 00:00:00

abstract：:Bioreactors are essential in tissue engineering, not only because they provide an in vitro environment mimicking in vivo conditions for the growth of tissue substitutes, but also because they enable systematic studies of the responses of living tissues to various mechanical and biochemical cues. The basic principles o...

journal_title：Biotechnology letters

authors： Chen HC,Hu YC

更新日期：2006-09-01 00:00:00

abstract：:A Simplified version of rate equations for enzyme-catalyzed reactions has been developed in which the rate equation is analyzed with two different mechanisms: ordered bi-ter and Ping-Pong bi-bi mechanisms. This procedure is able to develop the rate expressions accurately. Random sequential order mechanism can be effec...

journal_title：Biotechnology letters

authors： Thilakavathi M,Basak T,Panda T

更新日期：2006-12-01 00:00:00

abstract：OBJECTIVE:In this study, we aimed to maximize glutathione (GSH) production by a metabolically engineered Yarrowia lipolytica strain using a small-scale optimization approach. RESULTS:A three levels four factorial Box-Behnken Design was used to assess the effect of pH, inulin extract, yeast extract and ammonium sulfate...

journal_title：Biotechnology letters

authors： Do DTH,Fickers P,Ben Tahar I

更新日期：2021-02-01 00:00:00

abstract：OBJECTIVES:Establish a complete and efficient method for the preparation of cis-5-hydroxy-L-pipecolic acids (cis-5HPA), including biotransformation and isomers separation and purification. RESULTS:For non-heme Fe(II)/α-KG-dependent dioxygenases, α-ketoglutarate (α-KG) has great influence on the stability of Fe(II) ion...

journal_title：Biotechnology letters

authors： Lu F,Chen J,Ye H,Wu H,Sha F,Huang F,Cao F,Wei P

更新日期：2020-12-01 00:00:00

abstract：:5-Aminolevulinic acid (ALA) is an intermediate in the biosynthesis of tetrapyrroles. Its current production is expensive. We have developed a low-cost medium for Propionibacterium acidipropionici to produce extracellular ALA. When grown at 35 °C on a medium containing 3 % (w/v) food-grade sodium lactate supplemented w...

journal_title：Biotechnology letters

authors： Sonhom R,Thepsithar C,Jongsareejit B

更新日期：2012-09-01 00:00:00

abstract：:Lentiviral vectors have been used for gene transfer into the liver but their ability to efficiently transduce quiescent hepatocytes remains controversial. Lentivirus-mediated gene transfer is more efficient in cycling cells. We determine the effect of H-IL6 in the lentiviral transduction. The lentiviral vector was use...

journal_title：Biotechnology letters

authors： Picanço-Castro V,Fontes AM,Heinz S,Tonn T,Covas DT

更新日期：2008-02-01 00:00:00

abstract：:Clostridium spp. produce n-butanol in the acetone/butanol/ethanol process. For sustainable industrial scale butanol production, a number of obstacles need to be addressed including choice of feedstock, the low product yield, toxicity to production strain, multiple-end products and downstream processing of alcohol mixt...

journal_title：Biotechnology letters

authors： Jurgens G,Survase S,Berezina O,Sklavounos E,Linnekoski J,Kurkijärvi A,Väkevä M,van Heiningen A,Granström T

更新日期：2012-08-01 00:00:00

abstract：:The effects of a water-miscible ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF(4)]), on both thermodynamics and kinetic mechanism of the horseradish peroxidase (HRP)-catalyzed oxidation of guaiacol (2-methoxyphenol) by H(2)O(2) were investigated. The ionic liquid stabilized the ground state of ...

journal_title：Biotechnology letters

authors： Hong ES,Kwon OY,Ryu K

更新日期：2008-03-01 00:00:00

abstract：:In this study, 3D culture system for human adipose-derived stem cell (hASC) using a BioLevitator as the bioreactor for microcarrier-based cultures was established. During the culturing period, hASCs preferred to grow in crevices between microcarriers and a high viability was maintained even when reaching confluency. A...

journal_title：Biotechnology letters

authors： Lin CY,Huang CH,Wu YK,Cheng NC,Yu J

更新日期：2014-07-01 00:00:00

abstract：:Environmental stresses are the major cause of crop loss worldwide. Polyamines are involved in plant stress responses. However, the precise role(s) of polyamine metabolism in these processes remain ill-defined. Transgenic approaches demonstrate that polyamines play essential roles in stress tolerance and open up the po...

journal_title：Biotechnology letters

authors： Alcázar R,Marco F,Cuevas JC,Patron M,Ferrando A,Carrasco P,Tiburcio AF,Altabella T

更新日期：2006-12-01 00:00:00

abstract：OBJECTIVE:To investigate the aerotolerance of Lactobacillus rhamnosus hsryfm 1301 and its influencing factors. RESULTS:The growth rate of L. rhamnosus hsryfm 1301 weakened noticeably when the concentration of supplemented H2O2 reached 1 mM, and only 2% of all L. rhamnosus hsryfm 1301 cells survived in MRS broth supple...

journal_title：Biotechnology letters

authors： Zhang C,Lu J,Yang D,Chen X,Huang Y,Gu R

更新日期：2018-04-01 00:00:00

abstract：PURPOSE OF WORK:To apply a fluorescent dye as an alternative technique to evaluate the survival of potentially probiotic lactobacilli to bile acids (BA) as first step in the design of probiotic functional foods. The use of lactic acid bacteria (LAB) in the functional food design depends on their ability to survive in t...

journal_title：Biotechnology letters

authors： Bustos AY,Raya R,Bru E,de Valdez GF,Taranto MP

更新日期：2011-07-01 00:00:00

abstract：:Microgravity can influence cell growth and function. A transfected Sp2/0 myeloma cell line P3A2 producing a human IgG1 anti-TNFa monoclonal antibody was cultivated in static culture, spinner flasks and simulated microgravity using a rotating wall vessel bioreactor. Microgravity significantly decreased cell growth (fro...

journal_title：Biotechnology letters

authors： Foster LJ,Catzel D,Atwa S,Zarka M,Mahler SM

更新日期：2003-08-01 00:00:00

abstract：:Genomic DNA was isolated from as little as 2 mg dry biomass of Magnaporthe grisea by microwave treatment within 30 s. The quantity of DNA was good enough for PCR analysis and Dot blot hybridization. This technique can be used for various studies, such as DNA fingerprinting to study the population structure of the phyt...

journal_title：Biotechnology letters

authors： Tendulkar SR,Gupta A,Chattoo BB

更新日期：2003-11-01 00:00:00

abstract：OBJECTIVES:This short commentary examines the factors that led to Food and Drug Administration's approval of the first plant-derived biologic. RESULTS:In 2012, the first plant-derived protein pharmaceutical (biologic) was approved for commercial use in humans. The product, a recombinant form of human β-glucocerebrosid...

journal_title：Biotechnology letters

authors： Mor TS

更新日期：2015-11-01 00:00:00

abstract：:The old Annurca apple cultivar (Malus domestica), particularly appreciated for its peculiar flavor and crispy flesh, was studied in order to preserve its ancient germplasm. Twelve clones of Annurca were analyzed using random amplified polymorphic DNA (RAPD) and simple-sequence repeat (SSR) markers. Two out of 30 RAPD ...

journal_title：Biotechnology letters

authors： Iannaccone M,Palumbo D,Ventimiglia I,Patocchi A,Spigno P,Capparelli R

更新日期：2007-02-01 00:00:00

abstract：:Since T cells express diverse sex steroid hormone receptors, they might be a good model to evaluate the effects of sex steroid hormones on immune modulation. Porcine testicular extract contains several sex steroid hormones and may be useful to study the effects of sex steroid hormones during T cell activation. We have...

journal_title：Biotechnology letters

authors： Lee HH,Lee SJ,Kim S,Jeong S,Na M,Lee DM,Cheon YP,Lee KH,Choi I,Chun T

更新日期：2012-07-01 00:00:00

abstract：OBJECTIVES:To investigate mycothiol peroxidase (MPx) of Corynebacterium glutamicum that is a novel CysGPx family peroxidase using both the mycoredoxin and thioredoxin reducing systems as proton donors for peroxide detoxification and may be involved in the relief of acid stress. RESULTS:A Δmpx mutant exhibited signific...

journal_title：Biotechnology letters

authors： Wang T,Gao F,Kang Y,Zhao C,Su T,Li M,Si M,Shen X

更新日期：2016-07-01 00:00:00

abstract：:The basic reactions of the clostridial 1-butanol biosynthesis pathway can be regarded to be the inverted reactions of the fatty acid β-oxidation pathway. A pathway for the biosynthesis of fuels and chemicals was recently engineered by combining enzymes from both aerobic and anaerobic fatty acid β-oxidation as well as ...

journal_title：Biotechnology letters

authors： Gulevich AY,Skorokhodova AY,Sukhozhenko AV,Shakulov RS,Debabov VG

更新日期：2012-03-01 00:00:00