Abstract:
:Heterologous expression in Escherichia coli often leads to production of the expressed proteins as insoluble and inactive inclusion bodies. The general strategy for protein recovery includes isolation and washing of inclusion bodies, solubilization of aggregated protein and refolding of solubilized protein. The process of refolding, as well as the other steps involved in inclusion body recovery, must be optimized according to the characteristics of each protein. For the development of reliable and inexpensive serodiagnostic tests, the antigenic domain 1 (AD-1) of human cytomegalovirus glycoprotein B was expressed in E. coli and a process was developed to increase recovery of the fusion protein containing AD-1. A comparison of disruption methods and different conditions involved in recovery of this fusion protein from inclusion bodies is presented. The developed method gives a high yield of the fusion protein with a purity sufficient for use in diagnostic tests.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Ferreira S,Sousa F,Queiroz JA,Domingues FCdoi
10.1007/s10529-005-0024-xkeywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
1241-5issue
16eissn
0141-5492issn
1573-6776journal_volume
27pub_type
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