Responses of the antioxidative and biotransformation enzymes in the aquatic fungus Mucor hiemalis exposed to cyanotoxins.

Abstract:

OBJECTIVES:To investigate antioxidative and biotransformation enzyme responses in Mucor hiemalis towards cyanotoxins considering its use in mycoremediation applications. RESULTS:Catalase (CAT), glutathione reductase (GR), and glutathione peroxidase (GPx) in M. hiemalis maintained their activities at all tested microcystin-LR (MC-LR) exposure concentrations. Cytosolic glutathione S-transferase (GST) activity decreased with exposure to 100 µg MC-LR l-1 while microsomal GST remained constant. Cylindrospermopsin (CYN) at 100 µg l-1 led to an increase in CAT activity and inhibition of GR, as well as to a concentration-dependent GPx inhibition. Microsomal GST was inhibited at all concentrations tested. β-N-methylamino-L-alanine (BMAA) inhibited GR activity in a concentration-dependent manner, however, CAT, GPx, and GST remained unaffected. CONCLUSIONS:M. hiemalis showed enhanced oxidative stress tolerance and intact biotransformation enzyme activity towards MC-LR and BMAA in comparison to CYN, confirming its applicability in bioreactor technology in terms of viability and survival in their presence.

journal_name

Biotechnol Lett

journal_title

Biotechnology letters

authors

Balsano E,Esterhuizen-Londt M,Hoque E,Lima SP

doi

10.1007/s10529-017-2348-8

subject

Has Abstract

pub_date

2017-08-01 00:00:00

pages

1201-1209

issue

8

eissn

0141-5492

issn

1573-6776

pii

10.1007/s10529-017-2348-8

journal_volume

39

pub_type

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