Abstract:
OBJECTIVES:To investigate antioxidative and biotransformation enzyme responses in Mucor hiemalis towards cyanotoxins considering its use in mycoremediation applications. RESULTS:Catalase (CAT), glutathione reductase (GR), and glutathione peroxidase (GPx) in M. hiemalis maintained their activities at all tested microcystin-LR (MC-LR) exposure concentrations. Cytosolic glutathione S-transferase (GST) activity decreased with exposure to 100 µg MC-LR l-1 while microsomal GST remained constant. Cylindrospermopsin (CYN) at 100 µg l-1 led to an increase in CAT activity and inhibition of GR, as well as to a concentration-dependent GPx inhibition. Microsomal GST was inhibited at all concentrations tested. β-N-methylamino-L-alanine (BMAA) inhibited GR activity in a concentration-dependent manner, however, CAT, GPx, and GST remained unaffected. CONCLUSIONS:M. hiemalis showed enhanced oxidative stress tolerance and intact biotransformation enzyme activity towards MC-LR and BMAA in comparison to CYN, confirming its applicability in bioreactor technology in terms of viability and survival in their presence.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Balsano E,Esterhuizen-Londt M,Hoque E,Lima SPdoi
10.1007/s10529-017-2348-8subject
Has Abstractpub_date
2017-08-01 00:00:00pages
1201-1209issue
8eissn
0141-5492issn
1573-6776pii
10.1007/s10529-017-2348-8journal_volume
39pub_type
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