Responses of the antioxidative and biotransformation enzymes in the aquatic fungus Mucor hiemalis exposed to cyanotoxins.


OBJECTIVES:To investigate antioxidative and biotransformation enzyme responses in Mucor hiemalis towards cyanotoxins considering its use in mycoremediation applications. RESULTS:Catalase (CAT), glutathione reductase (GR), and glutathione peroxidase (GPx) in M. hiemalis maintained their activities at all tested microcystin-LR (MC-LR) exposure concentrations. Cytosolic glutathione S-transferase (GST) activity decreased with exposure to 100 µg MC-LR l-1 while microsomal GST remained constant. Cylindrospermopsin (CYN) at 100 µg l-1 led to an increase in CAT activity and inhibition of GR, as well as to a concentration-dependent GPx inhibition. Microsomal GST was inhibited at all concentrations tested. β-N-methylamino-L-alanine (BMAA) inhibited GR activity in a concentration-dependent manner, however, CAT, GPx, and GST remained unaffected. CONCLUSIONS:M. hiemalis showed enhanced oxidative stress tolerance and intact biotransformation enzyme activity towards MC-LR and BMAA in comparison to CYN, confirming its applicability in bioreactor technology in terms of viability and survival in their presence.


Biotechnol Lett


Biotechnology letters


Balsano E,Esterhuizen-Londt M,Hoque E,Lima SP




Has Abstract


2017-08-01 00:00:00














  • Fed-batch versus batch cultures of Yarrowia lipolytica for γ-decalactone production from methyl ricinoleate.

    abstract::Constant medium feeding rate and intermittent fed-batch fermentation strategies were investigated aiming to increase the yields of γ-decalactone production by Yarrowia lipolytica, using methyl ricinoleate as substrate and ricinoleic acid source. The accumulation of another compound, 3-hydroxy-γ-decalactone, was also a...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Gomes N,Teixeira JA,Belo I

    更新日期:2012-04-01 00:00:00

  • Anthocyanic vacuolar inclusions (AVIs) selectively bind acylated anthocyanins in Vitis vinifera L. (grapevine) suspension culture.

    abstract::Anthocyanic vacuolar inclusions (AVIs) appear as dark red-to-purple spheres of various sizes in vacuoles of grapevine (Vitis vinifera L.) cell suspension culture due to their interaction with anthocyanins. AVIs were purified and the bound anthocyanins extracted and analysed by HPLC from two lines of V. vinifera isolat...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Conn S,Zhang W,Franco C

    更新日期:2003-06-01 00:00:00

  • High-level expression of non-glycosylated and active staphylokinase from Pichia pastoris.

    abstract::Staphylokinase (SAK) is a promising thrombolytic agent for treating blood-clotting disorders. Recombinant SAK (rSAK) was produced after integration of the gene into Pichia pastoris genome. The recombinant Pichia carrying multiple insertions of the SAK gene yielded high-level (approximately 1 g/l) of extracellular glyc...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Apte-Deshpnade A,Mandal G,Soorapaneni S,Prasad B,Kumar J,Padmanabhan S

    更新日期:2009-06-01 00:00:00

  • Discovery of novel inhibitors for human intestinal maltase: virtual screening in a WISDOM environment and in vitro evaluation.

    abstract::Human intestinal maltase (HMA) is an α-glucosidase that hydrolyses α-1,4-linkages from the non-reducing end of malto-oligosaccharides. HMA is an important target to discover of new drugs for the treatment of type 2 diabetes. In this study, 308,307 compounds were virtually screened with HMA using Autodock 3.0.5 in a WI...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Nguyen TT,Ryu HJ,Lee SH,Hwang S,Cha J,Breton V,Kim D

    更新日期:2011-11-01 00:00:00

  • Transgenic tomatoes expressing human beta-amyloid for use as a vaccine against Alzheimer's disease.

    abstract::Human beta-amyloid (Abeta) is believed to be one of the main components of Alzheimer's disease, so reduction of Abeta is considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for Abeta with tandem repeats. Integration of the human Abeta gene into the...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Youm JW,Jeon JH,Kim H,Kim YH,Ko K,Joung H,Kim H

    更新日期:2008-10-01 00:00:00

  • Xylose catabolism by a mutant ofStreptomyces chrysomallus altered in the regulation of D-glucose isomerase.

    abstract::A mutant ofS. chrysomallus with a D-glucose isomerase inducible by D-glucose or its catabolites was characterized. In contrast to the wild-type strain it showed a decreased catabolite repression by D-glucose of D-xylose consumption and a constitutive pentose phosphate pathway as well. A hypothesis concerning altered i...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Hänel F,Roth M,Neigenfind M,Gräfe U,Bormann EJ

    更新日期:1987-12-01 00:00:00

  • Genetically-modified R-ω-transaminase: purification and self-assembly facilitating interaction with substrate droplets.

    abstract:OBJECTIVES:An easy-to-operate method of using R-ω-transaminase has been developed by fusing it to an elastin-like polypeptide and forming a complex with D-amino acid oxidase. RESULTS:R-ω-Transaminase (R-ω-TA) was fused to an elastin-like polypeptide (ELP) through genetic engineering of the enzyme. The enzyme was purif...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Gao Q,Fu Y,Peng Y,Liu W,Feng W

    更新日期:2016-03-01 00:00:00

  • Nanoformulation of curcumin protects HUVEC endothelial cells against ionizing radiation and suppresses their adhesion to monocytes: potential in prevention of radiation-induced atherosclerosis.

    abstract:OBJECTIVES:To investigated the potential of a novel dendrosomal nanoformulation of curcumin (DNC) in blocking radiation-induced changes in irradiated human umbilical vein endothelial cells (HUVECs), and their adhesion to human THP-1 monocytoid cells. RESULTS:Co60 gamma rays reduced viability, raised the expression of ...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Soltani B,Bodaghabadi N,Mahpour G,Ghaemi N,Sadeghizadeh M

    更新日期:2016-12-01 00:00:00

  • Increased expression of OsPT1, a high-affinity phosphate transporter, enhances phosphate acquisition in rice.

    abstract::Most high-affinity phosphate transporter genes (OsPTs) in rice were highly induced in roots when phosphate was depleted. OsPT1, however, was highly expressed in primary roots and leaves regardless of external phosphate concentrations. This finding was confirmed histochemically using transgenic rice plants that express...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Seo HM,Jung Y,Song S,Kim Y,Kwon T,Kim DH,Jeung SJ,Yi YB,Yi G,Nam MH,Nam J

    更新日期:2008-10-01 00:00:00

  • Alginate oligosaccharide protects endothelial cells against oxidative stress injury via integrin-α/FAK/PI3K signaling.

    abstract::Alginate oligosaccharide (AOS) was reported to possess antioxidant and free radical scavenging activities, but the specific effects and mechanisms remain unclear. We investigated the effects of AOS on H2O2-induced oxidative stress and apoptosis in human umbilical vein endothelial cells (HUVECs) and the associated mech...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Zhao J,Han Y,Wang Z,Zhang R,Wang G,Mao Y

    更新日期:2020-12-01 00:00:00

  • Characterization of the hydrolysate and catalytic cavity of α-agarase AgaD.

    abstract:OBJECTIVE:To characterize the hydrolysis product and the substrate binding in the catalytic cavity of α-agarase AgaD. RESULTS:The time course curve showed that AgaD degraded agarose by the endo-type cleavage. AgaD did not degrade agarobiose (A2) and agarotetraose (A4). The minimum-length substrate was agarohexaose (A6...

    journal_title:Biotechnology letters

    pub_type: 杂志文章,评审


    authors: Wang H,Zhang W,Cui Z,Lu Z,Lu X

    更新日期:2020-10-01 00:00:00

  • Therapeutic potential of human umbilical cord blood mesenchymal stem cells on erectile function in rats with cavernous nerve injury.

    abstract:OBJECTIVE:To evaluate the therapeutic potential of human umbilical cord blood mesenchymal stem cells (hUCBMSCs) on promoting erectile function in a rat model of bilateral cavernous nerve (CN) crush injury. RESULTS:Fifty male Sprague-Dawley rats were randomly assigned to sham + PBS group (n = 10), BCNI (bilateral caver...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Zhu JQ,Lu HK,Cui ZQ,Wang YC,Li YH,Zhao W,Fu Q,Xu YM,Xu Y,Song LJ

    更新日期:2015-07-01 00:00:00

  • Degradation of lignin-containing materials by xylanase in biopreparation of cotton.

    abstract::Solubilization of lignin and carbohydrates from the lignin-holocellulose structure of cotton seed-coat fragments was investigated by UV/VIS spectrometry. Xylanase (Pulpzyme HC) pre-treatment partially destroyed the lignocellulosic structure of the seed-coat fragments, producing reducing sugars and soluble lignin in th...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Csiszár E,Losonczi A,Koczka B,Szakács G,Pomlényi A

    更新日期:2006-05-01 00:00:00

  • Nucleotide mutations in purA gene and pur operon promoter discovered in guanosine- and inosine-producing Bacillus subtilis strains.

    abstract::The promoter region of the pur operon, which contains 12 genes for inosine monophosphate biosynthesis from phosphoribosylpyrophosphate, and the purA gene, encoding the adenylosuccinate synthetase, were compared among wild-type and three purine-producing Bacillus subtilis strains. A single nucleotide deletion at positi...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Qian J,Cai X,Chu J,Zhuang Y,Zhang S

    更新日期:2006-06-01 00:00:00

  • Characterization of a novel cry2Aa-type gene from Bacillus thuringiensis subsp. kurstaki.

    abstract::A 4 kb BamHI-HindIlI fragment, corresponding to the cry2A operon of Bacillus thuringiensis subsp. kurstaki strain BNS3, was cloned. The sequencing of the corresponding cry2Aa-type gene, termed crybns3-4, revealed an open reading frame of 1902 bp, encoding a protein of 633 amino-acid residues. Both nucleotide and amino...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Tounsi S,Jaoua S

    更新日期:2003-08-01 00:00:00

  • Induction of endophytic colonization in rice (Oryza sativa L.) tissue culture plants by Azorhizobium caulinodans.

    abstract::Endophytic colonization in rice was induced using rhizobia. Dehusked seeds of rice hybrid, CORH2, were used as explants for induction of calli. MS medium was modified with 2,4-D (2.5 mg l(-1)) and kinetin (0.2 mg l(-1)) for callus induction. Well-developed calli were inoculated with Azorhizobium caulinodans strains OR...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Senthilkumar M,Madhaiyan M,Sundaram SP,Sangeetha H,Kannaiyan S

    更新日期:2008-08-01 00:00:00

  • Selective control of cyanobacteria by surfactin-containing culture broth of Bacillus subtilis C1.

    abstract::Of several types of chemical surfactants and biosurfactants, only the culture broth of Bacillus subtilis C1 containing surfactin at 10 mg l(-1) completely inhibited the growth of Microcystis aeruginosa, a bloom-forming cyanobacterium in highly eutrophic lakes. The broth with 10 mg surfactin l(-1) also removed 85% of t...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Ahn CY,Joung SH,Jeon JW,Kim HS,Yoon BD,Oh HM

    更新日期:2003-07-01 00:00:00

  • Hydrogen bio-production through anaerobic microorganism fermentation using kitchen wastes as substrate.

    abstract::In order to treat the kitchen wastes and produce hydrogen, anaerobic fermentation technology was used in this experiment. The results showed that the fermentation type changed from mixed acid fermentation to ethanol fermentation in a continuous stirred tank reactor (CSTR) 22 days after start-up. The maximum efficiency...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Shi Y,Zhao XT,Cao P,Hu Y,Zhang L,Jia Y,Lu Z

    更新日期:2009-09-01 00:00:00

  • Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells.

    abstract::The exposure of CHO DG44 cells to an osmotic shock, after DNA uptake, results in a cellular volume decrease of approx. 55%. Repetitive osmotic shocks targeted different sub-populations of cells as was demonstrated using two different fluorescent reporter genes. Also the exposure of a calcium phosphate-DNA coprecipitat...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Grosjean F,Bertschinger M,Hacker DL,Wurm FM

    更新日期:2006-11-01 00:00:00

  • Overexpression of a soybean O-acetylserine (thiol) lyase-encoding gene GmOASTL4 in tobacco increases cysteine levels and enhances tolerance to cadmium stress.

    abstract::O-Acetylserine (thiol) lyase (OASTL, EC catalyzes the final step in cysteine biosynthesis. The soybean GmOASTL4 gene was overexpressed in tobacco. Transgenic plants showed markedly increased accumulation of transcripts and higher cysteine content compared with the wild-type. Upon exposure to cadmium stress, ...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Ning H,Zhang C,Yao Y,Yu D

    更新日期:2010-04-01 00:00:00

  • Extending the linker region increases the activity of the Bacillus subtilis cellulase CelI15.

    abstract:OBJECTIVES:To investigate the effect of the linker region (LR) on the enzymatic activity, stability, and flexibility of Bacillus subtilis cellulase CelI15, six mutants were constructed that contained increasing numbers of the LR. RESULTS:The CelI15 mutant with three copies of the LR (approx. 57 amino acids) showed the...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Li P,Zhou Y,Li Q,Zhang C,Sun Z,Tian L,Weng H

    更新日期:2016-09-01 00:00:00

  • Over-expression of recombinant human phospholipid scramblase 1 in E. coli and its purification from inclusion bodies.

    abstract::Human phospholipid scramblase 1 (hPLSCR1) scrambles plasma membrane phospholipids during cell activation, blood coagulation and apoptosis. It was over-expressed in E. coli with a histidine tag and purified from the inclusion bodies (*30 mg/l culture broth) under denaturing conditions using 8 M urea. The denatured hPLS...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Sahu SK,Gopala Krishna A,Gummadi SN

    更新日期:2008-12-01 00:00:00

  • Effects of 5-azacytidine-induced DNA demethylation on polyketide synthase gene expression in larvae of sea urchin Strongylocentrotus intermedius.

    abstract:OBJECTIVE:To investigate the role of cytosine methylation in regulation of polyketide compounds biosynthesis in larvae of Strongylocentrotus intermedius. RESULTS:Treatment of S. intermedius larvae with 100 and 200 µM 5-azacytidine (5A) as a DNA demethylating agent significantly increased the amounts of spinochrome D a...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Tyunin AP,Ageenko NV,Kiselev KV

    更新日期:2016-12-01 00:00:00

  • Chemoporation using saponins or cholates: an alternative method for transformation of bacterial cells.

    abstract::A new method for fast transformation of competent bacterial cells has been developed. The transformation is induced with cholic acid analogues or saponins which cause reversible disruption of the bacterial membrane. This method shortens the time of transformation without significant loss of transformation efficiency i...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Ravnikar M,Irman A,Radić N,Lunder M,Strukelj B

    更新日期:2009-12-01 00:00:00

  • High cell density cultivation of Brevibacterium linens and formation of proteinases and lipase.

    abstract::Brevibacterium linens forms hydrolytic enzymes which can be used to accelerate the ripening of cheese without causing bitterness. B. linens ATCC 9172 was grown to a high cell density (50 g dry wt l-1 after 60 h) in a mineral medium containing lactic acid, soy-peptone and ammonium sulphate by applying a continuous feed...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Adamitsch BF,Karner F,Hampel WA

    更新日期:2003-05-01 00:00:00

  • 3D in vitro co-culture models based on normal cells and tumor spheroids formed by cyclic RGD-peptide induced cell self-assembly.

    abstract:OBJECTIVES:To design novel 3D in vitro co-culture models based on the RGD-peptide-induced cell self-assembly technique. RESULTS:Multicellular spheroids from M-3 murine melanoma cells and L-929 murine fibroblasts were obtained directly from monolayer culture by addition of culture medium containing cyclic RGD-peptide. ...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Akasov R,Gileva A,Zaytseva-Zotova D,Burov S,Chevalot I,Guedon E,Markvicheva E

    更新日期:2017-01-01 00:00:00

  • Anti-prelog reduction of prochiral carbonyl compounds by Oenococcus oeni in a biphasic system.

    abstract::An aqueous-organic biphasic system was established and used with whole cells of Oenococcus oeni to reduce 2-octanone to (R)-2-octanol. The conversion reached 99% when the Tris/borate buffer was increased from 50 mM to 300 mM in the aqueous phase. In addition, the conversion increased as the log P value of the organic ...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Hu J,Xu Y

    更新日期:2006-07-01 00:00:00

  • Unexpected reversal of the regioselectivity in Thermomyces lanuginosus lipase-catalyzed acylation of floxuridine.

    abstract::Unexpected inversion of the 3':5'-regioselectivity was observed in the enzymatic methacryloylation, crotonylation and cinnamoylation of floxuridine (1.5:1, 2.3:1 and 4.4:1, respectively), where Thermomyces lanuginosus lipase preferentially catalyzed the acylation of 3'-hydroxyl rather than that of 5'-hydroxyl group. T...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Li N,Zong MH,Ma D

    更新日期:2009-08-01 00:00:00

  • Enhanced production of recombinant nattokinase in Bacillus subtilis by the elimination of limiting factors.

    abstract::By systematic investigation, glutamate and a mixture of metal ions were identified as factors limiting the production of nattokinase in Bacillus subtilis. Consequently, in medium supplemented with these materials, the recombinant strain secreted 4 times more nattokinase (260 mg l(-1)) than when grown in the unsuppleme...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Chen PT,Chao YP

    更新日期:2006-10-01 00:00:00

  • Biosynthetic origin of the carbon skeleton of a novel anti-tumor compound, haloroquinone, from a marine-derived fungus, Halorosellinia sp.

    abstract:PURPOSE OF WORK:The biosynthetic pathway of a new antitumor compound, haloroquinone, is elucidated to facilitate metabolic regulation for product accumulation and modification to produce new bioactive structural analogues of the compound. The biosynthetic origin of a novel promising protein kinase B inh...

    journal_title:Biotechnology letters

    pub_type: 杂志文章


    authors: Niu C,Cai M,Zhang Y,Zhou X

    更新日期:2012-11-01 00:00:00