Application of fluorescent techniques to evaluate the survival of probiotic lactobacilli to bile acid.

abstract：:The effects of the sequential application of methyl jasmonate (MeJa), salicylic acid (SA) and yeast extract (YE) to Argemone mexicana cell cultures were compared to either the sole application of each elicitor, or to the three-partite mixture. The highest sanguinarine accumulation occurred using the sequential treatme...

journal_title：Biotechnology letters

authors： Trujillo-Villanueva K,Rubio-Piña J,Monforte-González M,Ramírez-Benítez E,Vázquez-Flota F

更新日期：2012-02-01 00:00:00

abstract：:Serratia marcescens SS-1 and its SpnR-defective isogenic mutant, SMdeltaR, produced an extracellular surfactant able to decrease surface tension of water from 72 to 37 dyne cm(-1) (SMdeltaR strain) and to 45 dyne cm(-1) (SS-1 strain). The biosurfactant also emulsified kerosene and diesel with a maximum emulsion index ...

journal_title：Biotechnology letters

authors： Wei YH,Lai HC,Chen SY,Yeh MS,Chang JS

更新日期：2004-05-01 00:00:00

abstract：:An extracellular esterase, EstK, was purified from the psychrotrophic bacterium Pseudomonas mandelii grown at 25°C. Prior to harvest, cells were treated with 0.2 M MgCl2 to precipitate lipopolysaccharides in the outer membranes, which otherwise form aggregates with the secreted enzymes. EstK was purified to homogeneit...

journal_title：Biotechnology letters

authors： Hong S,Lee C,Jang SH

更新日期：2012-06-01 00:00:00

abstract：:Fourteen yeast strains were screened for production of 2-phenylethanol from L-phenylalanine with molasses as carbon source. Up to 1 g 2-phenylethanol l-1 was obtained. Using oleyl alcohol as a second phase for in situ product removal to enhance the production of 2-phenylethanol increased the yield to about 3 g 2-pheny...

journal_title：Biotechnology letters

authors： Etschmann MM,Sell D,Schrader J

更新日期：2003-04-01 00:00:00

abstract：:Unexpected inversion of the 3':5'-regioselectivity was observed in the enzymatic methacryloylation, crotonylation and cinnamoylation of floxuridine (1.5:1, 2.3:1 and 4.4:1, respectively), where Thermomyces lanuginosus lipase preferentially catalyzed the acylation of 3'-hydroxyl rather than that of 5'-hydroxyl group. T...

journal_title：Biotechnology letters

authors： Li N,Zong MH,Ma D

更新日期：2009-08-01 00:00:00

abstract：:An endoxylanase gene, xynA, was cloned from Bacillus pumilus ARA and expressed in Escherichia coli. The open reading frame of the xynA gene was 687 bp encoding a signal peptide and a mature xylanase with a molecular mass of 23 kDa. The enzyme was categorized as a glycosyl hydrolase family 11 member based on the sequen...

journal_title：Biotechnology letters

authors： Qu W,Shao W

更新日期：2011-07-01 00:00:00

abstract：:Fusion expression is a common practice for recombinant protein production. Some fusion tags confer solubility on the target protein whereas others provide affinity handles that facilitate purification. However, the tag usually needs to be removed from the final product, which involves using expensive proteases or haza...

journal_title：Biotechnology letters

authors： Li Y

更新日期：2011-05-01 00:00:00

abstract：OBJECTIVES:To improve the efficiency, reproducibility and consistency of the PEI-based transfection method that is often used in preparation of recombinant lentiviral or retroviral vectors. RESULTS:The contributions to transfection efficiency of multi-factors including concentration of PEI or DNA, dilution buffer for ...

journal_title：Biotechnology letters

authors： Yang S,Shi H,Chu X,Zhou X,Sun P

更新日期：2016-09-01 00:00:00

abstract：:The extracellular domains (ECD) of epidermal growth factor receptors, ErbB1, 2, 3 and 4, were designed as soluble dimeric forms. Each ECD was fused to a short hinge region derived from IgG, such that the stable dimer could be formed with disulfide bridges. This hinge-tagged design minimized the molecular weight to app...

journal_title：Biotechnology letters

authors： Otani T,Hashizume T,Nagaoka T,Fukuda T,Tang CK,Salomon DS,Seno M

更新日期：2010-03-01 00:00:00

abstract：:Tandem affinity purification (TAP) is a methodology for the isolation of protein complexes from endogenous sources. It involves incorporation of a dual-affinity tag into the protein of interest and introduction of the construct into desired cell lines or organisms. Using the two affinity handles, the protein complex a...

journal_title：Biotechnology letters

authors： Li Y

更新日期：2011-08-01 00:00:00

abstract：:Fungal diseases damage crop plants and affect agricultural production. Transgenic plants have been produced by inserting antifungal genes to confer resistance against fungal pathogens. Genes of fungal cell wall-degrading enzymes, such as chitinase and glucanase, are frequently used to produce fungal-resistant transgen...

journal_title：Biotechnology letters

authors： Ceasar SA,Ignacimuthu S

更新日期：2012-06-01 00:00:00

abstract：OBJECTIVE:To investigate the role of cytosine methylation in regulation of polyketide compounds biosynthesis in larvae of Strongylocentrotus intermedius. RESULTS:Treatment of S. intermedius larvae with 100 and 200 µM 5-azacytidine (5A) as a DNA demethylating agent significantly increased the amounts of spinochrome D a...

journal_title：Biotechnology letters

authors： Tyunin AP,Ageenko NV,Kiselev KV

更新日期：2016-12-01 00:00:00

abstract：:Biodesulfurization (BDS) in a bioreactor packed with a catalytic bed of silica containing immobilized Rhodococcus rhodochrous was studied. Various bed lengths and support particle sizes were evaluated for BDS of dibenzothiophene (DBT) and gas oil. The sulfur-containing substrates were introduced separately into the bi...

journal_title：Biotechnology letters

authors： Alejandro Dinamarca M,Orellana L,Aguirre J,Baeza P,Espinoza G,Canales C,Ojeda J

更新日期：2014-08-01 00:00:00

abstract：:Three biological activities of 12 soy isoflavones were investigated in terms of the structure-activity relationship. Seven molecular descriptors and one absorption predictor were significantly related to the inhibition of cytochrome P4501 activity in HepG2 cells by soy isoflavones, yielding water solubility [correlati...

journal_title：Biotechnology letters

authors： Chun HS,Chang HJ,Choi EH,Kim HJ,Ku KH

更新日期：2005-08-01 00:00:00

abstract：:High concentrations of 2,4,6-trinitrotoluene (TNT) and related nitroaromatic compounds are commonly found in soil and groundwater at former explosive plants. The bacterium, Raoultella terrigena strain HB, isolated from a contaminated site, converts TNT into the corresponding amino products. Radio-HPLC analysis with [(...

journal_title：Biotechnology letters

authors： Claus H,Perret N,Bausinger T,Fels G,Preuss J,König H

更新日期：2007-03-01 00:00:00

abstract：:The transfer of the IncP-7 carbazole degradative plasmid pCAR1 from Pseudomonas putida SM1443 (derived from strain KT2440) into bacteria of river water samples was monitored using a reporter gene encoding red fluorescent protein (RFP). The number of transconjugants drastically increased in the presence of carbazole, a...

journal_title：Biotechnology letters

authors： Shintani M,Fukushima N,Tezuka M,Yamane H,Nojiri H

更新日期：2008-01-01 00:00:00

abstract：OBJECTIVES:To investigated the potential of a novel dendrosomal nanoformulation of curcumin (DNC) in blocking radiation-induced changes in irradiated human umbilical vein endothelial cells (HUVECs), and their adhesion to human THP-1 monocytoid cells. RESULTS:Co60 gamma rays reduced viability, raised the expression of ...

journal_title：Biotechnology letters

authors： Soltani B,Bodaghabadi N,Mahpour G,Ghaemi N,Sadeghizadeh M

更新日期：2016-12-01 00:00:00

abstract：OBJECTIVES:Interactions of cells with their neighbors and influences by the surrounding extracellular matrix (ECM) is reflected in a cells transcriptome and proteome. In tissues comprised of heterogeneous cell populations or cells depending on ECM signalling cues such as those of the intervertebral disc (IVD), this inf...

journal_title：Biotechnology letters

authors： Li K,Varden L,Henderson A,Lufkin T,Kraus P

更新日期：2021-01-01 00:00:00

abstract：:Image analysis was used to measure the effect of Tween 40 on the morphology of Trichoderma harzianum. The percentage of pellets was maximal (93%) with Tween at 0.2 ml l(-1), whereas the maximal proportion of dispersed mycelia (40%) was with Tween at 1.6 ml l(-1). The particle median diameter decreased from 2 to 0.5 mm...

journal_title：Biotechnology letters

authors： Lucatero S,Galindo E,Larralde-Corona CP

更新日期：2004-01-01 00:00:00

abstract：:Our current knowledge of protein unfolding is overwhelmingly related to reversible denaturation. However, to engineer thermostable enzymes for industrial applications and medical diagnostics, it is necessary to consider irreversible denaturation processes and/or the entire quaternary structure. In this study we have u...

journal_title：Biotechnology letters

authors： Kaneko H,Minagawa H,Shimada J

更新日期：2005-11-01 00:00:00

abstract：:A gene, rBmK Cta, encoding a chlorotoxin-like peptide from the scorpion, Buthus martensii Karsch, was synthesized according to the sequence optimized for codon usage in Escherichia coli and was expressed in E. coli BL21 (DE3) using a pExSecI expression system in which the IgG-binding domain-ZZ of protein A is fused to...

journal_title：Biotechnology letters

authors： Fu YJ,Yin LT,Wang W,Chai BF,Liang AH

更新日期：2005-10-01 00:00:00

abstract：:Mucor circinelloides and Mortierella isabellina hydroxylated dehydroabietic acid (DehA). DehA was converted regio- and stereo-selectively by whole cells of Mr. circinelloides to give 2alpha-hydroxydehydroabietic acid in a 75% molar conversion yield (11 mM from 14.7 mM DehA) after 72 h in the cultivation medium contain...

journal_title：Biotechnology letters

authors： Mitsukura K,Imoto T,Nagaoka H,Yoshida T,Nagasawa T

更新日期：2005-09-01 00:00:00

abstract：:Environmental stresses are the major cause of crop loss worldwide. Polyamines are involved in plant stress responses. However, the precise role(s) of polyamine metabolism in these processes remain ill-defined. Transgenic approaches demonstrate that polyamines play essential roles in stress tolerance and open up the po...

journal_title：Biotechnology letters

authors： Alcázar R,Marco F,Cuevas JC,Patron M,Ferrando A,Carrasco P,Tiburcio AF,Altabella T

更新日期：2006-12-01 00:00:00

abstract：:Lymphotactin-XCL1 is a chemokine produced mainly by activated CD8+ T-cells and directs migration of CD4+ and CD8+ lymphocytes and natural killer (NK) cells. We expressed human lymphotactin (LTN) by the lactic-acid bacterium Lactococcus lactis. Biological activity of LTN was confirmed by chemo-attraction of human T-cel...

journal_title：Biotechnology letters

authors： Zavala-Flores LM,Villatoro-Hernandez J,Gamez-Escobedo A,Franco-Molina M,Rangel-Colmenero BR,Villanueva-Olivo A,Gutierrez-Puente Y,de Oca-Luna RM,Valdés-Flores J,Saucedo-Cardenas O

更新日期：2009-02-01 00:00:00

abstract：:The white-rot fungus Trametes versicolor grown in submerged culture produced two laccase isoenzymes, LacI and LacII. Addition of insoluble lignocellulosic materials into the culture medium increased the total laccase activity. The proportion of laccase isoenzymes also changed depending on the lignocellulosic material ...

journal_title：Biotechnology letters

authors： Moldes D,Lorenzo M,Sanromán MA

更新日期：2004-02-01 00:00:00

abstract：OBJECTIVES:To design novel 3D in vitro co-culture models based on the RGD-peptide-induced cell self-assembly technique. RESULTS:Multicellular spheroids from M-3 murine melanoma cells and L-929 murine fibroblasts were obtained directly from monolayer culture by addition of culture medium containing cyclic RGD-peptide. ...

journal_title：Biotechnology letters

authors： Akasov R,Gileva A,Zaytseva-Zotova D,Burov S,Chevalot I,Guedon E,Markvicheva E

更新日期：2017-01-01 00:00:00

abstract：OBJECTIVES:The purpose of this study was to develop a facile and efficient method to enhance the stability and activity of lactoperoxidase (LPO) by using its immobilization on graphene oxide nanosheets (GO-NS). METHODS:Following the LPO purification from bovine whey, it was immobilized onto functionalized GO-NS using ...

journal_title：Biotechnology letters

authors： Shariat SZAS,Borzouee F,Mofid MR,Varshosaz J

更新日期：2018-10-01 00:00:00

abstract：:5-Aminolevulinic acid (ALA) is an intermediate in the biosynthesis of tetrapyrroles. Its current production is expensive. We have developed a low-cost medium for Propionibacterium acidipropionici to produce extracellular ALA. When grown at 35 °C on a medium containing 3 % (w/v) food-grade sodium lactate supplemented w...

journal_title：Biotechnology letters

authors： Sonhom R,Thepsithar C,Jongsareejit B

更新日期：2012-09-01 00:00:00

abstract：:Continuous production of fructooligosaccharides (FOS) by Aureobasidium pullulans immobilized on calcium alginate beads with a packed bed was investigated at a plant scale reactor. Optimum conditions were with 770 g sucrose/l, being fed at 200 l/h at 50°C which gave a productivity of 180 g FOS/l h. Initial activity was...

journal_title：Biotechnology letters

authors： Jung KH,Bang SH,Oh TK,Park HJ

更新日期：2011-08-01 00:00:00

abstract：:Tempe was prepared using mixtures of natural soybean and genetically modified Roundup Ready (RUR) soybean fermented with natural Rhizopus sp. The amount of RUR soybean was quantified using an ELISA plate test. The RUR signal decreased during fermentation. In the control experiments on fermentation of non-RUR soybean, ...

journal_title：Biotechnology letters

authors： Prakoso B,Nitisinprasert S,Stevens WF

更新日期：2003-09-01 00:00:00