Production of biologically active human lymphotactin (XCL1) by Lactococcus lactis.

abstract：:A single-compartmented microbial fuel cell composed of a graphite felt anode modified with Neutral Red (NR-anode) and a porous Fe(II)-carbon cathode (FeC-cathode) were compared for electricity generation from Microbacterium sp. and Pseudomonas sp. under identical conditions. Pseudomonas sp. was more than four times th...

journal_title：Biotechnology letters

authors： Lee SW,Jeon BY,Park DH

更新日期：2010-04-01 00:00:00

abstract：:Ramoplanin is a lipopeptide antibiotic active against multi-drug-resistant, Gram-positive pathogens. Structurally, it contains a di-mannose moiety attached to the peptide core at Hpg(11). The biosynthetic gene cluster of ramoplanin has already been reported and the assembly of the depsipeptide has been elucidated but ...

journal_title：Biotechnology letters

authors： Chen JS,Wang YX,Shao L,Pan HX,Li JA,Lin HM,Dong XJ,Chen DJ

更新日期：2013-09-01 00:00:00

abstract：:A fully contained and efficient heterologous protein production system was designed using Brassica rapa rapa (turnip) hairy roots. Two expression cassettes containing a cauliflower mosaic virus (CaMV) 35S promoter with a duplicated enhancer region, an Arabidopsis thaliana sequence encoding a signal peptide and the CaM...

journal_title：Biotechnology letters

authors： Huet Y,Ekouna JP,Caron A,Mezreb K,Boitel-Conti M,Guerineau F

更新日期：2014-01-01 00:00:00

abstract：:Growth of Streptococcus zooepidemicus in a 10 l bioreactor with 50 g sucrose/l and 10 g casein hydrolysate/l gave 5-6 g hyaluronic acid/l after 24-28 h. Purification of hyaluronic acid gave a recovery of 65% with the final material having an Mr of approximately 4 x 10(6) Da with less than 0.1% protein. ...

journal_title：Biotechnology letters

authors： Rangaswamy V,Jain D

更新日期：2008-03-01 00:00:00

abstract：:Trichodesmium sp., isolated from the Great Barrier Reef lagoon, was cultured in artificial seawater media containing a range of Fe concentration. Fe additions stimulated growth, N2 fixation, cellular chlorophyll a content, light-saturated chlorophyll a-specific gross photosynthetic capacity (Pmchl a) and the dark resp...

journal_title：Biotechnology letters

authors： Fu FX,Bell PR

更新日期：2003-04-01 00:00:00

abstract：:In vitro biocatalysis with cytochrome P450 BM-3 was investigated aiming for the substitution of the expensive natural cofactor NADPH by electrochemistry. The monooxygenase was immobilized on electrodes by entrapment in polypyrrole as a conductive polymer for electrochemically wiring the enzyme. Electropolymerization o...

journal_title：Biotechnology letters

authors： Holtmann D,Mangold KM,Schrader J

更新日期：2009-05-01 00:00:00

abstract：:Synthesis of long DNA fragments is often associated with mutations and requires multiple DNA manipulation steps. A novel DNA synthesis method, referred to as patch oligodeoxynucleotide synthesis (POS) to assembly long DNA fragments is presented here. This method involves connection of two types of oligodeoxynucleotide...

journal_title：Biotechnology letters

authors： Yang G,Wang S,Wei H,Ping J,Liu J,Xu L,Zhang W

更新日期：2012-04-01 00:00:00

abstract：:The aim of this study was to characterize the growth of the fungus Leucoagaricus gongylophorus LEU18496, isolated from the fungus garden of the nest of leaf cutter ants Atta mexicana. The fungus garden was cultivated in an artificial laboratory nest and the fungus further grown in submerged (SmC) and solid state (SSC)...

journal_title：Biotechnology letters

authors： Maya-Yescas ME,Revah S,Le Borgne S,Valenzuela J,Palacios-González E,Terrés-Rojas E,Vigueras-Ramírez G

更新日期：2021-01-02 00:00:00

abstract：OBJECTIVES:To rationally identify targets for enhancing adenosine production, transcription level of genes involved in adenosine synthesis of Bacillus subtilis XGL was detected during the fermentation process, complemented with metabolite pool analysis. RESULTS:PurR-regulated genes (pur operon and purA) and prs were d...

journal_title：Biotechnology letters

authors： Zhang C,Du S,Liu Y,Xie X,Xu Q,Chen N

更新日期：2015-07-01 00:00:00

abstract：:General methods for identification of usual carotenoids including C18-HPLC retention time and spectroscopic methods, such as absorption spectra, mass spectra, NMR spectra, are briefly summarized. ...

journal_title：Biotechnology letters

authors： Takaichi S

更新日期：2014-06-01 00:00:00

abstract：:The culture broth of Streptomyces halstedii AJ-7 suppressed the growth of Phytophthora capsici, which causes phytophthora blight in red-peppers, with less than 1% survival of the pathogen after 12 h of treatment. The low molecular fraction (< or = 10 kDa) of the culture broth retained anti-fungal activity against P. c...

journal_title：Biotechnology letters

authors： Joo GJ

更新日期：2005-02-01 00:00:00

abstract：:Aptamers against inactive Vibrio alginolyticus were selected from an 82-nt ssDNA random library by systematic evolution of ligands by exponential enrichment. After 15 rounds of selection, the final pool of aptamers was highly specific for inactivated V. alginolyticus and had a dissociation constant of 27.5 ± 9.2 nM. U...

journal_title：Biotechnology letters

authors： Tang X,Zheng J,Yan Q,Li Z,Li Y

更新日期：2013-06-01 00:00:00

abstract：:Washed cells (62 mg) of Kitasatospora sp. GF12 in 4 ml buffer (pH 7) catalyzed the regioselective hydroxylation of 60 mM 1,3-adamantanediol [1,3-ad(OH)(2)] to 30.9 mM 1,3,5-adamantanetriol [1,3,5-ad(OH)(3)] over 120 h at 24 °C. Glycerol at 400 mM was added to the reaction mixture to recycle the intracellular NADH/NADP...

journal_title：Biotechnology letters

authors： Mitsukura K,Yamanaka N,Yoshida T,Nagasawa T

更新日期：2012-09-01 00:00:00

abstract：:Two home, blood-glucose monitoring meters, OneTouch Ultra and Ascensia Contour, were used to determine the glucose concentration during fermentations of Trichoderma reesei in both flasks and bioreactors. The results, when compared to those given by the 3,5-dinitrosalicylic acid reducing sugar assay, HPLC and YSI 2700 ...

journal_title：Biotechnology letters

authors： Choy V,Patel N,Thibault J

更新日期：2007-07-01 00:00:00

abstract：:Batch fermentation by Streptomyces pristinaespiralis with the addition of adsorbent resins was used to increase the production of pristinamycin. In consideration of the adsorption capacity and the desorption ability, a polymeric resin, JD-1, was finally selected. The maximum production of pristinamycin in Erlenmeyer f...

journal_title：Biotechnology letters

authors： Jia B,Jin ZH,Lei YL,Mei LH,Li NH

更新日期：2006-11-01 00:00:00

abstract：:The combined analysis of peptide mass fingerprinting and 2-DE/MS using the induced and selected protein spots following growth of Pseudomonas sp. DU102 on benzoate or p-hydroxybenzoate revealed not only alpha- and beta-subunits of protocatechuate 3,4-dioxygenase but also catechol 1,2-dioxygenase responsible for ortho-...

journal_title：Biotechnology letters

authors： Kim SI,Park SH,Kim JW,Leem SH,Shin DJ,Kim SH,Lee DH,Kahng HY

更新日期：2007-10-01 00:00:00

abstract：:The production of a low-temperature alkalophilic lipase from Acinetobacter johnsonii was improved using genome shuffling. The starting populations, obtained by UV irradiation and diethyl sulfate mutagenesis, were subjected to recursive protoplast fusion. The optimal conditions for protoplast formation and regeneration...

journal_title：Biotechnology letters

authors： Wang H,Zhang J,Wang X,Qi W,Dai Y

更新日期：2012-01-01 00:00:00

abstract：:5-Aminolevulinic acid (ALA) is an intermediate in the biosynthesis of tetrapyrroles. Its current production is expensive. We have developed a low-cost medium for Propionibacterium acidipropionici to produce extracellular ALA. When grown at 35 °C on a medium containing 3 % (w/v) food-grade sodium lactate supplemented w...

journal_title：Biotechnology letters

authors： Sonhom R,Thepsithar C,Jongsareejit B

更新日期：2012-09-01 00:00:00

abstract：:We truncated the VP2 protein of infectious bursal disease virus into five fragments: V1-5. All fragments were displayed on the inner membrane of the Escherichia coli periplasm. After disruption of the outer membrane, spheroplasts that had anchored with the VP2 fragment were incubated with an anti-VP2 polyclonal antibo...

journal_title：Biotechnology letters

authors： Guo M,Xu LM,Zhou B,Yin JC,Ye XL,Ren GP,Li DS

更新日期：2014-03-01 00:00:00

abstract：:Dibutyl phthalate (DBP) was more efficiently degraded by cutinase compared to yeast esterase; i.e. almost 80% of initial DBP (500 mg l(-1)) was decomposed within 7.5 h, and nearly 50% of the degraded DBP disappeared within the initial 30 min. The toxicity of the final DBP degradation products were investigated using v...

journal_title：Biotechnology letters

authors： Kim YH,Lee J

更新日期：2005-05-01 00:00:00

abstract：:Microbial desulfurization is potentially an alternative process to chemical desulfurization of fossil fuels and their refined products. The dibenzothiophene desulfurizing system of Rhodococcus erythropolis includes DszD which is an NADH-dependent FMN oxidoreductase with 192 residues that is responsible for supplying r...

journal_title：Biotechnology letters

authors： Kamali N,Tavallaie M,Bambai B,Karkhane AA,Miri M

更新日期：2010-07-01 00:00:00

abstract：:An acoustic cell settler (ACS) using ultrasound at cells of 3 MHz was used to recycle Saccharomyces cerevisiae in a fermenter. The locations of both the inlet and outlet in the acoustic cell settler, which have a relatively long distance between the transducer and reflector, were optimized. A tilted settler was design...

journal_title：Biotechnology letters

authors： Hwang SH,Koo YM

更新日期：2003-02-01 00:00:00

abstract：:The industrial-scale production of phenylalanine ammonia-lyase (PAL) mainly uses strains of Rhodotorula. However, the PAL gene from Rhodotorula has not been cloned. Here, the full-length gene of PAL from Rhodotorula glutinis was isolated. It was 2,121 bp, encoding a polypeptide with 706 amino acids and a calculated MW...

journal_title：Biotechnology letters

authors： Zhu L,Cui W,Fang Y,Liu Y,Gao X,Zhou Z

更新日期：2013-05-01 00:00:00

abstract：:A chip was developed to store DNA for medical research. The optional restriction site fixed on the chip can randomly ligate with whole human genomic DNA treated by the corresponding restriction enzyme. PCR can then use the chip as template DNA. Moreover, a chip fixing two restriction sites (e.g. EcoRI and HindIII) sho...

journal_title：Biotechnology letters

authors： Hanaoka T,Takai O,Takahashi K,Tsugane S

更新日期：2003-03-01 00:00:00

abstract：:A carboxylesterase gene from thermophilic bacterium, Alicyclobacillus tengchongensis, was cloned and expressed in Escherichia coli BL21 (DE3). The gene coded for a 513 amino acid protein with a calculated molecular mass of 57.82 kDa. The deduced amino acid sequence had structural features highly conserved among serine...

journal_title：Biotechnology letters

authors： Xie Z,Xu B,Ding J,Liu L,Zhang X,Li J,Huang Z

更新日期：2013-08-01 00:00:00

abstract：OBJECTIVES:To examine the synergistic antibacterial activity of violacein and silver nanoparticles (AgNPs) against ATCC bacteria, Staphylococcus aureus, Escherichia coli and two bacteria isolated from bovine mastitis. METHODS:Violacein from Chromobacterium violaceum and biogenic AgNPs from Fusarium oxysporum were eval...

journal_title：Biotechnology letters

authors： Nakazato G,Gonçalves MC,da Silva das Neves M,Kobayashi RKT,Brocchi M,Durán N

更新日期：2019-12-01 00:00:00

abstract：:Excretion of 1,3-propanediol (1,3-PD) by K. pneumoniae was compared in ammonium- and phosphate-limited chemostat cultures running with an excess of glycerol. 59 and 43% catabolic flux were directed to 1,3-PD in ammonia-limited cultures and phosphate-limited cultures at dilution rate of 0.1 h(-1), respectively. Ammonia...

journal_title：Biotechnology letters

authors： Zheng ZM,Xu YZ,Wang TP,Dong CQ,Yang YP,Liu DH

更新日期：2010-02-01 00:00:00

abstract：:Streptomyces is an attractive host for heterologous protein secretion. To further optimize its expression capacity, better expression vectors will be helpful. Here, based on pSGL1, a high copy number plasmid present in Streptomyces globisporus C-1027, we constructed a series of novel E. coli-Streptomyces shuttle expre...

journal_title：Biotechnology letters

authors： Zhu Y,Wang L,Du Y,Wang S,Yu T,Hong B

更新日期：2011-02-01 00:00:00

abstract：:Some pest management programs employ PCR to identify cry1 genes from Bacillus thuringiensis to predict bacterial toxicity towards different insect pests. However, due to changes on the mode of action of the Cry proteins, new primers had to be designed to detect the new genes. Therefore, an 'in-silico' study of genetic...

journal_title：Biotechnology letters

authors： Davolos CC,Guidelli-Thuler AM,de Abreu IL,Sena JA,Lemos MV

更新日期：2009-12-01 00:00:00

abstract：:Several alkyl-substituted methoxysilanes were evaluated as potential activity and stability enhancing agents for biomimetic silicification of Rhodosporidium toruloides D-amino acid oxidase (RtDAO). When methyl-substituted silanes along with tetramethoxysilane were used as silicic acid precursors for polyallylamine (PA...

journal_title：Biotechnology letters

authors： Kuan IC,Chuang CA,Lee SL,Yu CY

更新日期：2012-08-01 00:00:00