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Alkyl-substituted methoxysilanes enhance the activity and stability of D-amino acid oxidase encapsulated in biomimetic silica.

Abstract：

:Several alkyl-substituted methoxysilanes were evaluated as potential activity and stability enhancing agents for biomimetic silicification of Rhodosporidium toruloides D-amino acid oxidase (RtDAO). When methyl-substituted silanes along with tetramethoxysilane were used as silicic acid precursors for polyallylamine (PAA)--or R5 peptide-catalyzed silicic encapsulation, the RtDAO activity increased with the degree of substitution and the molar ratio up to 15 % of methyl-substituted silanes added. In the presence of 15 mol% trimethylmethoxysilane, the specific activities of encapsulated RtDAO catalyzed by PAA and R5 increased by 1.4- and 4.8-fold, respectively. For PAA-catalyzed encapsulation, a 2.4-fold increase occurred with 30 mol% n-propyltrimethoxysilane; this modification increased the T (m) value by 10 °C and gave a threefold longer half-life in the presence of 10 mM H(2)O(2) as compared to the encapsulation using tetramethoxysilane only.

journal_name

Biotechnol Lett

journal_title

Biotechnology letters

authors

Kuan IC,Chuang CA,Lee SL,Yu CY

doi

10.1007/s10529-012-0924-5

subject

Has Abstract

pub_date

2012-08-01 00:00:00

pages

1493-8

issue

8

eissn

0141-5492

issn

1573-6776

journal_volume

34

pub_type

杂志文章

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