Abstract:
:Microbes employ cell membranes for reducing exogenous stresses. Polyamino acid display on microbial cell surfaces and their effects on microbial chemical stress tolerance were examined. Growth analysis revealed that displays of polyarginine, polyaspartate and polytryptophan substantially enhanced tolerance of Escherichia coli to NaCl. A titration assay indicated that polyarginine and polyaspartate altered cell surface charges, implying tolerance enhancement via ion atmosphere and/or ionic bond network formations for electrostatic ion repulsion. The enhancement by polytryptophan may have arisen from surface hydrophobicity increase for hydrophobic ion exclusion, because of a strong correlation between hydrophobic characters of amino acids and their effects on tolerance enhancement. The display also enhanced tolerance to other salts and/or alcohols in E. coli and to NaCl in Saccharomyces cerevisiae. Thus polyamino acid display has the potential as an approach for conferring chemical stress tolerance on various microbes.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Suzuki H,Ishii J,Kondo A,Yoshida Kdoi
10.1007/s10529-014-1689-9subject
Has Abstractpub_date
2015-02-01 00:00:00pages
429-35issue
2eissn
0141-5492issn
1573-6776journal_volume
37pub_type
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